BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144541.4 - phase: 0
(412 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78573 similar to GP|20197309|gb|AAC63626.2 unknown protein {Ar... 590 e-169
TC83851 similar to GP|15027849|gb|AAK76455.1 unknown protein {Ar... 328 2e-90
TC76600 similar to GP|20197309|gb|AAC63626.2 unknown protein {Ar... 242 2e-64
TC91850 similar to GP|22597166|gb|AAN03470.1 RING-H2 finger prot... 40 0.002
TC88854 similar to GP|7767667|gb|AAF69164.1| F27F5.26 {Arabidops... 40 0.002
TC85903 similar to PIR|B84530|B84530 probable RING zinc finger p... 39 0.003
TC78547 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly e... 39 0.003
TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16... 39 0.004
TC78520 similar to PIR|T47637|T47637 hypothetical protein T5N23.... 38 0.006
TC88172 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly e... 38 0.006
TC90788 similar to PIR|F86340|F86340 protein F2D10.34 [imported]... 38 0.008
TC88616 weakly similar to PIR|T48209|T48209 hypothetical protein... 37 0.014
TC91231 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidop... 37 0.014
AJ499045 similar to PIR|F86340|F86 protein F2D10.34 [imported] -... 37 0.014
TC84165 similar to GP|21539497|gb|AAM53301.1 putative protein {A... 37 0.018
BF648985 similar to PIR|T51245|T512 COP1-interacting protein CIP... 36 0.024
BF646214 similar to GP|8843779|dbj| gene_id:MZN1.3~unknown prote... 36 0.031
BF634100 similar to GP|18377636|gb| unknown protein {Arabidopsis... 35 0.041
TC90096 similar to PIR|T47832|T47832 hypothetical protein T2O9.6... 35 0.053
TC79350 similar to PIR|T08944|T08944 hypothetical protein F25O24... 35 0.053
>TC78573 similar to GP|20197309|gb|AAC63626.2 unknown protein {Arabidopsis
thaliana}, partial (31%)
Length = 2016
Score = 590 bits (1522), Expect = e-169
Identities = 283/415 (68%), Positives = 328/415 (78%), Gaps = 7/415 (1%)
Frame = +2
Query: 5 IDDGDGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQC 64
+D+G+ KS SV CSICLE+V+DNGDRSFAKL CGHQFHLDCIGSAFN KGAMQC
Sbjct: 230 VDEGN------KSLGSVSCSICLEVVTDNGDRSFAKLLCGHQFHLDCIGSAFNIKGAMQC 391
Query: 65 PNCRKIEKGQWLYANGSRSYPEFNMEDWTRDDDVYDLSYSEMSLGVHWCPFGNFAQLPSS 124
PNCRKIEKGQWLYANGSRSYPEF+M+DWT D+D+YDLSYSEMS GVHWCPFGN A+LPSS
Sbjct: 392 PNCRKIEKGQWLYANGSRSYPEFSMDDWTHDEDLYDLSYSEMSFGVHWCPFGNMARLPSS 571
Query: 125 FEEREYSPIPYHDAPG--PMFTEHSAVSSGSHPCPYIAYVGPVHPSTSNSGGTVSEVSNF 182
FEE E+S I Y D G F EH+AVSS SHPCPY+AY GP+HPS+SNSGG VSE NF
Sbjct: 572 FEEGEFSSIAYPDIIGQHAAFAEHTAVSSASHPCPYVAYFGPIHPSSSNSGGAVSEAPNF 751
Query: 183 NHWNARPIHGDMSTSYTVPAVVLHYHSWDHHSSHFSSGSSHLGAADQPSVSQSNQRPTR- 241
NHWN + +M SYT PAV LHYHSW+HH+ FS+ SS L ADQPSVS ++QRP +
Sbjct: 752 NHWNGSSVPSEMPASYTFPAVDLHYHSWEHHTPPFSTASSRLVGADQPSVSPASQRPVKG 931
Query: 242 GSEATRSGSYMHPYPVGHSSVARAGNSAASSMIPPYPGSNARARDRVEALQAYYHQQQPP 301
GS+ RSGS+MHP+ VGHSS ARAG+S SSMIPPYPGSNARARDRV+ALQAYY QQ P
Sbjct: 932 GSDVPRSGSFMHPFLVGHSSAARAGSSMGSSMIPPYPGSNARARDRVQALQAYYQPQQHP 1111
Query: 302 NSTTVRPPVASSARRSSGHSRSAPLASLSSPPDQSGSYIYLP----GRNFQEETRLPSHF 357
NSTT+R PV+S RR+S HS S LA +++ PDQSG + +P GRNFQEE LPS F
Sbjct: 1112NSTTMRAPVSSVPRRASSHSGSTQLAPVATSPDQSGGFFLIPSSSSGRNFQEENHLPSRF 1291
Query: 358 HAWERDHLPSSSLNQVGRESSWRAYHQTASGTDPGFRSSSFRLRSDSERTPSQNR 412
HAWERDHLPS SL+Q R+S WR YHQ+A+ +DP RS++FRLR S+R PSQ+R
Sbjct: 1292HAWERDHLPSLSLSQGDRDSGWRPYHQSANRSDPSTRSNNFRLRHGSDRMPSQHR 1456
>TC83851 similar to GP|15027849|gb|AAK76455.1 unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 674
Score = 328 bits (842), Expect = 2e-90
Identities = 176/251 (70%), Positives = 183/251 (72%), Gaps = 6/251 (2%)
Frame = +1
Query: 127 EREYSPIPYHDAPGPMFTEHSAVSSGSHPCPYIAYVGPVHPSTSNSGGTVSEVSNFNHWN 186
EREYSPIPYHDAPGPMFTEHSAVS SEVSNFNHWN
Sbjct: 13 EREYSPIPYHDAPGPMFTEHSAVS--------------------------SEVSNFNHWN 114
Query: 187 ARPIHGDMSTSYTVPAVVLHYHSWDHHSSHFSSGSSHLGAADQPSVSQSNQRPTRGSEAT 246
ARPIHGDMSTSYTVPAVVLHYHSWDHHSSHFSSGSSHLGAADQPSVSQSNQRPTRGSEAT
Sbjct: 115 ARPIHGDMSTSYTVPAVVLHYHSWDHHSSHFSSGSSHLGAADQPSVSQSNQRPTRGSEAT 294
Query: 247 RSGSYMHPYPVGHSSVARAGNSAASSMIPPYPGSNARARDRVEALQAYYHQQQPP----N 302
RSGSYMHPYPVGHSSVARAGNSAASSMIPPYPGSNARAR+RVEALQAYYHQQQP +
Sbjct: 295 RSGSYMHPYPVGHSSVARAGNSAASSMIPPYPGSNARARNRVEALQAYYHQQQPSKFYHS 474
Query: 303 STTVRPPVASSARRSSGHSRSAPLASLSSPPDQSGSYIYLPGRN--FQEETRLPSHFHAW 360
+ T R + G L P Q+ ++ FQEETRLPSH HAW
Sbjct: 475 TATCRFLALEDPAVTVGQ-----LH*HRCPRRQTKVVATFISQDAIFQEETRLPSHXHAW 639
Query: 361 ERDHLPSSSLN 371
ERDHLPSS LN
Sbjct: 640 ERDHLPSSXLN 672
Score = 75.9 bits (185), Expect = 3e-14
Identities = 45/66 (68%), Positives = 46/66 (69%)
Frame = +3
Query: 312 SSARRSSGHSRSAPLASLSSPPDQSGSYIYLPGRNFQEETRLPSHFHAWERDHLPSSSLN 371
SSARRSSGHSRSAPLASLSSPPDQSGSYIYLPGRNF R PS S SL
Sbjct: 492 SSARRSSGHSRSAPLASLSSPPDQSGSYIYLPGRNFSR--RNPS-----------SKSLX 632
Query: 372 QVGRES 377
VG+ S
Sbjct: 633 CVGKRS 650
>TC76600 similar to GP|20197309|gb|AAC63626.2 unknown protein {Arabidopsis
thaliana}, partial (17%)
Length = 505
Score = 242 bits (617), Expect = 2e-64
Identities = 111/111 (100%), Positives = 111/111 (100%)
Frame = +3
Query: 1 MDLNIDDGDGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKG 60
MDLNIDDGDGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKG
Sbjct: 171 MDLNIDDGDGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKG 350
Query: 61 AMQCPNCRKIEKGQWLYANGSRSYPEFNMEDWTRDDDVYDLSYSEMSLGVH 111
AMQCPNCRKIEKGQWLYANGSRSYPEFNMEDWTRDDDVYDLSYSEMSLGVH
Sbjct: 351 AMQCPNCRKIEKGQWLYANGSRSYPEFNMEDWTRDDDVYDLSYSEMSLGVH 503
>TC91850 similar to GP|22597166|gb|AAN03470.1 RING-H2 finger protein
{Glycine max}, partial (48%)
Length = 1054
Score = 39.7 bits (91), Expect = 0.002
Identities = 21/47 (44%), Positives = 26/47 (54%)
Frame = +3
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRK 69
C ICLE D GD+ LQCGH+FH C+ + G CP CR+
Sbjct: 609 CGICLESFMD-GDK-LIHLQCGHKFHSACLDPWIRSCG--DCPYCRR 737
>TC88854 similar to GP|7767667|gb|AAF69164.1| F27F5.26 {Arabidopsis
thaliana}, partial (22%)
Length = 1043
Score = 39.7 bits (91), Expect = 0.002
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = +2
Query: 18 SDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
S++ PC +C E + D L CGH +H+DCI K CP C+
Sbjct: 572 SETEPCCVCQEEFKNEDD--IGSLDCGHDYHIDCIKQWLTHKNI--CPICK 712
>TC85903 similar to PIR|B84530|B84530 probable RING zinc finger protein
[imported] - Arabidopsis thaliana, partial (26%)
Length = 2572
Score = 39.3 bits (90), Expect = 0.003
Identities = 21/52 (40%), Positives = 26/52 (49%)
Frame = +3
Query: 17 SSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
SSD PC IC E D ++ L CGH+FH +CI K CP C+
Sbjct: 2124 SSDLEPCCICQEEYVDG--QNLGLLDCGHEFHSNCITQWLMQKNL--CPICK 2267
Score = 30.4 bits (67), Expect = 1.3
Identities = 38/155 (24%), Positives = 63/155 (40%), Gaps = 8/155 (5%)
Frame = +2
Query: 251 YMHPYPVGHSSVARAGNSAASSMIPPYPGSNARARDRVEALQAYYHQQQPPNSTTVRP-- 308
+MHP+P ++ R S++S Y RD ++ + P +T P
Sbjct: 1304 HMHPFPWNRAANPRVARSSSS-----YSSGERAVRDD-------FNLRIFPRDSTEHPMN 1447
Query: 309 -PVASSARRSSGHSRSAPLASLSS--PPDQSGSYIYLPGRNFQEETRLPSHFHAWERDH- 364
P +S + +S S+ L + PP GS LP+ +W +H
Sbjct: 1448 MPASSGHEPAGWYSSSSNLNNAGGIPPPSWIGS--------SSNVHSLPN--PSWTFNHE 1597
Query: 365 LPSSSLNQVGRESSWRAYH--QTASGTDPGFRSSS 397
+P+ +L +V S W + +ASGT G SS+
Sbjct: 1598 VPTENLQRVSEFSPWSLFPSISSASGTHNGHSSST 1702
>TC78547 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly elicited
protein 132 {Nicotiana tabacum}, partial (41%)
Length = 1648
Score = 39.3 bits (90), Expect = 0.003
Identities = 22/68 (32%), Positives = 34/68 (49%)
Frame = +2
Query: 19 DSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKIEKGQWLYA 78
D + C++CL V + G+++ +C H FHLDCI F + CP CR + +
Sbjct: 614 DGLECAVCLCDVVE-GEKTRLLPKCNHGFHLDCIDMWFQSHST--CPLCRNLVSVESCKP 784
Query: 79 NGSRSYPE 86
N + S E
Sbjct: 785 NSTTSNGE 808
>TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16.190 -
Arabidopsis thaliana, partial (81%)
Length = 1378
Score = 38.9 bits (89), Expect = 0.004
Identities = 21/54 (38%), Positives = 26/54 (47%)
Frame = +2
Query: 15 RKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
+ S D + CS+CLE V N L C HQFH CI +G CP C+
Sbjct: 854 KASDDELTCSVCLEQV--NVGDVLRSLPCLHQFHASCIDPWLRQQGT--CPVCK 1003
>TC78520 similar to PIR|T47637|T47637 hypothetical protein T5N23.140 -
Arabidopsis thaliana, partial (71%)
Length = 2748
Score = 38.1 bits (87), Expect = 0.006
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Frame = +1
Query: 12 TRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAK-GAMQCPNCR 68
++ S C+ICL + ++ +C H FH CI S N K G CP CR
Sbjct: 328 SKSSSKSSKQTCAICLTKMKQGSGQAIFTAECSHSFHFHCIAS--NVKHGNQVCPVCR 495
>TC88172 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly elicited
protein 132 {Nicotiana tabacum}, partial (26%)
Length = 1445
Score = 38.1 bits (87), Expect = 0.006
Identities = 18/46 (39%), Positives = 26/46 (56%)
Frame = +3
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
CS+CL V + G++ +C H FH+DCI F++ CP CR
Sbjct: 492 CSVCLSEVVE-GEKVRVLPKCNHMFHIDCIDMWFHSHST--CPLCR 620
>TC90788 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana, partial (52%)
Length = 745
Score = 37.7 bits (86), Expect = 0.008
Identities = 21/60 (35%), Positives = 31/60 (51%)
Frame = +2
Query: 11 DTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKI 70
D+ K + C+ICL + GD QCGH FH+ CI + + + CP+CR+I
Sbjct: 299 DSNPGKWLATTECAICLSDFAA-GDEIRVLPQCGHGFHVACIDTWLGSHSS--CPSCRQI 469
>TC88616 weakly similar to PIR|T48209|T48209 hypothetical protein T20L15.150
- Arabidopsis thaliana, partial (27%)
Length = 1148
Score = 37.0 bits (84), Expect = 0.014
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Frame = +2
Query: 17 SSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR-KIE 71
S + V C++CL +V D + C H FH+ CI + CPNCR K+E
Sbjct: 485 SKNIVECAVCLSVVEDE-EMMRLLPNCKHSFHVGCIDKWLASHST--CPNCRTKVE 643
>TC91231 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidopsis
thaliana}, partial (17%)
Length = 728
Score = 37.0 bits (84), Expect = 0.014
Identities = 17/60 (28%), Positives = 24/60 (39%)
Frame = +1
Query: 9 DGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
D H + + C ICL D + +L CGH FH C+ + CP C+
Sbjct: 52 DSPIEHVLADEDAECCICLSAYDDGAE--LRQLPCGHHFHCTCVDKWLHINAT--CPLCK 219
>AJ499045 similar to PIR|F86340|F86 protein F2D10.34 [imported] - Arabidopsis
thaliana, partial (62%)
Length = 692
Score = 37.0 bits (84), Expect = 0.014
Identities = 19/48 (39%), Positives = 26/48 (53%)
Frame = +2
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKI 70
C+ICL + GD QCGH FH+ CI + + CP+CR+I
Sbjct: 371 CAICLSEFTA-GDEIRVLPQCGHGFHVSCIDLWLRSHSS--CPSCRQI 505
>TC84165 similar to GP|21539497|gb|AAM53301.1 putative protein {Arabidopsis
thaliana}, partial (16%)
Length = 792
Score = 36.6 bits (83), Expect = 0.018
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = +3
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAK-GAMQCPNCR 68
C+ICL + + +C H FH CI S N K G CP CR
Sbjct: 60 CAICLNTMKPGNGHAIFTAECSHSFHFHCITS--NVKHGNQICPVCR 194
>BF648985 similar to PIR|T51245|T512 COP1-interacting protein CIP8 [imported]
- Arabidopsis thaliana, partial (15%)
Length = 572
Score = 36.2 bits (82), Expect = 0.024
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = -3
Query: 18 SDSVPCSICLEIVSDNGDRSFAK-LQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
S+ V C++C E++ G AK L CGH++H DCI + + CP CR
Sbjct: 468 SEVVACAVCKEML---GVGXMAKRLPCGHEYHGDCIVPWLSTRNX--CPICR 328
>BF646214 similar to GP|8843779|dbj| gene_id:MZN1.3~unknown protein
{Arabidopsis thaliana}, partial (27%)
Length = 638
Score = 35.8 bits (81), Expect = 0.031
Identities = 24/69 (34%), Positives = 32/69 (45%)
Frame = +2
Query: 23 CSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCRKIEKGQWLYANGSR 82
C ICL D G+ +CGH FHL+CI ++ CP CR ++ N S
Sbjct: 380 CVICLSGFED-GEMGRCLPKCGHGFHLECIDMWLSSHS--NCPICR---ASIVVFENDSS 541
Query: 83 SYPEFNMED 91
S E N +D
Sbjct: 542 SVVENNGDD 568
>BF634100 similar to GP|18377636|gb| unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 376
Score = 35.4 bits (80), Expect = 0.041
Identities = 28/82 (34%), Positives = 35/82 (42%), Gaps = 4/82 (4%)
Frame = +2
Query: 4 NIDDGD---GDTRHRK-SSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAK 59
NID G G + R S + C ICL +N + +L C H FH DC+
Sbjct: 14 NIDGGTVAAGTEKERVISGEDAVCCICLAKYENNDE--LRELPCSHLFHKDCVDKWLKIN 187
Query: 60 GAMQCPNCRKIEKGQWLYANGS 81
CP C K E G+ L GS
Sbjct: 188AL--CPLC-KSEVGEDLTGLGS 244
>TC90096 similar to PIR|T47832|T47832 hypothetical protein T2O9.60 -
Arabidopsis thaliana, partial (13%)
Length = 676
Score = 35.0 bits (79), Expect = 0.053
Identities = 19/52 (36%), Positives = 26/52 (49%)
Frame = +1
Query: 17 SSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQCPNCR 68
S+ V C++C E VS D +L C H +H DCI + + CP CR
Sbjct: 358 SNGVVICAVCKETVSV--DEEAKQLPCDHLYHADCITPWLRIRSS--CPLCR 501
>TC79350 similar to PIR|T08944|T08944 hypothetical protein F25O24.10 -
Arabidopsis thaliana, partial (32%)
Length = 1698
Score = 35.0 bits (79), Expect = 0.053
Identities = 36/122 (29%), Positives = 54/122 (43%), Gaps = 9/122 (7%)
Frame = +3
Query: 17 SSDSVPCSICLEIVSDNGDRSFAKL--QCGHQFHLDCIGSAFNAKGAMQCPNCR-KI--- 70
S + CSICL S D +L +C H FH+DCI + CP CR K+
Sbjct: 456 SKQGLECSICL---SKFEDIEILRLLPKCKHAFHIDCIDHWLEKHSS--CPICRHKVNIE 620
Query: 71 EKGQWLYANGSRSY--PEFNMEDWT-RDDDVYDLSYSEMSLGVHWCPFGNFAQLPSSFEE 127
++ + Y+N R E N+E + R++D S S+G F + + F+E
Sbjct: 621 DQTTFAYSNSLRMLVSEESNIEIFVEREEDNNQHGSSRFSIG---SSFRKIGKATTIFKE 791
Query: 128 RE 129
E
Sbjct: 792 EE 797
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.128 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,524,138
Number of Sequences: 36976
Number of extensions: 264843
Number of successful extensions: 1519
Number of sequences better than 10.0: 131
Number of HSP's better than 10.0 without gapping: 1449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1503
length of query: 412
length of database: 9,014,727
effective HSP length: 98
effective length of query: 314
effective length of database: 5,391,079
effective search space: 1692798806
effective search space used: 1692798806
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144541.4