Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144541.14 - phase: 0 
         (63 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC78385 weakly similar to PIR|T21861|T21861 hypothetical protein...   125  4e-30
TC78386 similar to SP|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprote...   125  4e-30
TC78583 similar to GP|4097585|gb|AAD09518.1| NTGP4 {Nicotiana ta...    28  0.54
TC88814 homologue to PIR|T06447|T06447 GTP-binding protein - gar...    28  0.70
BF647865                                                               27  1.2
TC84868 homologue to GP|18376071|emb|CAD21099. hypothetical prot...    25  4.5
TC82106 similar to PIR|T49191|T49191 hypothetical protein MAA21....    25  5.9
TC88509 similar to PIR|T01826|T01826 microfibril-associated prot...    25  7.7

>TC78385 weakly similar to PIR|T21861|T21861 hypothetical protein F36F2.3 -
           Caenorhabditis elegans, partial (1%)
          Length = 1417

 Score =  125 bits (313), Expect = 4e-30
 Identities = 63/63 (100%), Positives = 63/63 (100%)
 Frame = +1

Query: 1   PNGERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNELLNFLNA 60
           PNGERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNELLNFLNA
Sbjct: 907 PNGERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNELLNFLNA 1086

Query: 61  SFD 63
           SFD
Sbjct: 1087SFD 1095


>TC78386 similar to SP|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprotein
           precursor [Contains: Dentin phosphoprotein (Dentin
           phosphophoryn) (DPP);, partial (2%)
          Length = 726

 Score =  125 bits (313), Expect = 4e-30
 Identities = 63/63 (100%), Positives = 63/63 (100%)
 Frame = +3

Query: 1   PNGERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNELLNFLNA 60
           PNGERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNELLNFLNA
Sbjct: 423 PNGERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNELLNFLNA 602

Query: 61  SFD 63
           SFD
Sbjct: 603 SFD 611


>TC78583 similar to GP|4097585|gb|AAD09518.1| NTGP4 {Nicotiana tabacum},
           partial (86%)
          Length = 1269

 Score = 28.5 bits (62), Expect = 0.54
 Identities = 14/49 (28%), Positives = 31/49 (62%), Gaps = 1/49 (2%)
 Frame = +3

Query: 12  LSKFLGRNKDDGVRRS-AVSGKKILLKLDKTKEDKEAESKRNELLNFLN 59
           L  +LGR   + +++  ++ G + +L  +KTK++K+   +  +LL+F+N
Sbjct: 546 LDDYLGRECPESLKQILSLCGNRCVLFDNKTKDEKKRSGQVQQLLSFVN 692


>TC88814 homologue to PIR|T06447|T06447 GTP-binding protein - garden pea,
            complete
          Length = 1183

 Score = 28.1 bits (61), Expect = 0.70
 Identities = 14/24 (58%), Positives = 15/24 (62%)
 Frame = -3

Query: 36   LKLDKTKEDKEAESKRNELLNFLN 59
            LKLDK    KE  +KRNE   FLN
Sbjct: 1175 LKLDK*IPSKETRTKRNESARFLN 1104


>BF647865 
          Length = 638

 Score = 27.3 bits (59), Expect = 1.2
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +2

Query: 25  RRSAVSGKKILLKLDKTKEDKEAESKRN 52
           +RS V      + LD++ EDKE E KRN
Sbjct: 218 KRSVVGACSPEVSLDESMEDKELEGKRN 301


>TC84868 homologue to GP|18376071|emb|CAD21099. hypothetical protein
           {Neurospora crassa}, partial (0%)
          Length = 288

 Score = 25.4 bits (54), Expect = 4.5
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = -3

Query: 20  KDDGVRRSAVSGKKILLKLDKTKEDK 45
           ++D VRR+   GKK+ L  DK + D+
Sbjct: 202 REDMVRRAIQEGKKLGLHFDKGRADR 125


>TC82106 similar to PIR|T49191|T49191 hypothetical protein MAA21.130 -
           Arabidopsis thaliana, partial (5%)
          Length = 990

 Score = 25.0 bits (53), Expect = 5.9
 Identities = 16/51 (31%), Positives = 26/51 (50%)
 Frame = +2

Query: 3   GERSSSPVQLSKFLGRNKDDGVRRSAVSGKKILLKLDKTKEDKEAESKRNE 53
           GE    PV+ ++     KD+       +G +I     +T EDK+A+ K+NE
Sbjct: 203 GELEPEPVRETELKPAPKDEA------AGSEI----QQTSEDKQAQKKKNE 325


>TC88509 similar to PIR|T01826|T01826 microfibril-associated protein homolog
           T15F16.8 - Arabidopsis thaliana, partial (83%)
          Length = 1306

 Score = 24.6 bits (52), Expect = 7.7
 Identities = 13/47 (27%), Positives = 26/47 (54%), Gaps = 2/47 (4%)
 Frame = +2

Query: 14  KFLGRNKDDGVRRSAVS--GKKILLKLDKTKEDKEAESKRNELLNFL 58
           + L R ++DG+RRS V    +K  L+  + K  K+   +R+ ++  +
Sbjct: 425 RMLWRREEDGLRRSCVREIRRKRFLRKRRRKRRKKRRRRRSLIMRLI 565


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.307    0.129    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,351,539
Number of Sequences: 36976
Number of extensions: 10658
Number of successful extensions: 55
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 55
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 55
length of query: 63
length of database: 9,014,727
effective HSP length: 39
effective length of query: 24
effective length of database: 7,572,663
effective search space: 181743912
effective search space used: 181743912
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 51 (24.3 bits)


Medicago: description of AC144541.14