BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144541.1 - phase: 0
(124 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76395 similar to SP|Q9ATF5|RL1X_CASSA Ribosomal protein L18a. ... 246 2e-66
TC77548 homologue to SP|Q9M9W1|R22B_ARATH 60S ribosomal protein ... 225 4e-60
TC88677 similar to SP|Q9M9W1|R22B_ARATH 60S ribosomal protein L2... 223 2e-59
AI737573 similar to SP|Q9FE58|R22C_ 60S ribosomal protein L22-3.... 104 8e-24
AL375608 homologue to SP|Q9FE58|R22C_ 60S ribosomal protein L22-... 47 4e-09
TC92159 weakly similar to GP|19069568|emb|CAD25953. similarity t... 27 1.6
TC78586 similar to PIR|T01041|T01041 hypothetical protein YUP8H1... 27 2.0
TC78448 similar to SP|Q42546|DPNP_ARATH 3'(2') 5'-bisphosphate n... 26 3.5
TC88548 similar to GP|20196934|gb|AAB86455.2 bZIP family transcr... 26 3.5
TC78116 similar to PIR|F86441|F86441 probable cytochrome P450 [i... 26 4.5
TC81914 similar to GP|10178206|dbj|BAB11630. beta-glucosidase {A... 26 4.5
TC78604 similar to PIR|E84456|E84456 probable acyl-CoA synthetas... 25 5.9
TC86000 phosphoinositide-specific phospholipase C [Medicago trun... 25 7.7
>TC76395 similar to SP|Q9ATF5|RL1X_CASSA Ribosomal protein L18a. [Sweet
chestnut] {Castanea sativa}, complete
Length = 1640
Score = 246 bits (627), Expect = 2e-66
Identities = 124/124 (100%), Positives = 124/124 (100%)
Frame = -3
Query: 1 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 60
MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT
Sbjct: 780 MSRGSAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVT 601
Query: 61 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 120
REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG
Sbjct: 600 REKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEG 421
Query: 121 EEED 124
EEED
Sbjct: 420 EEED 409
>TC77548 homologue to SP|Q9M9W1|R22B_ARATH 60S ribosomal protein L22-2.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (92%)
Length = 606
Score = 225 bits (573), Expect = 4e-60
Identities = 111/118 (94%), Positives = 115/118 (97%)
Frame = +3
Query: 7 AGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKI 66
+G KGKKKG +F IDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSV+VTREKSKI
Sbjct: 36 SGVKGKKKGTSFVIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVTREKSKI 215
Query: 67 TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEED 124
TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDR+IYELRYFNIAENEGEEED
Sbjct: 216 TVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNIYELRYFNIAENEGEEED 389
>TC88677 similar to SP|Q9M9W1|R22B_ARATH 60S ribosomal protein L22-2.
[Mouse-ear cress] {Arabidopsis thaliana}, complete
Length = 613
Score = 223 bits (567), Expect = 2e-59
Identities = 112/125 (89%), Positives = 118/125 (93%), Gaps = 1/125 (0%)
Frame = +3
Query: 1 MSRGSAAGAKGKKKGA-TFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSV 59
M R AAG+KGKKKG TFTIDCAKPVEDKIMDIASLEKFLQERIKVGGK GALGD+V+V
Sbjct: 48 MGRVDAAGSKGKKKGVITFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKPGALGDAVTV 227
Query: 60 TREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENE 119
+REKSKI VTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDR++YELRYFNIAENE
Sbjct: 228 SREKSKIAVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNVYELRYFNIAENE 407
Query: 120 GEEED 124
GEEED
Sbjct: 408 GEEED 422
>AI737573 similar to SP|Q9FE58|R22C_ 60S ribosomal protein L22-3. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (40%)
Length = 471
Score = 104 bits (260), Expect = 8e-24
Identities = 50/50 (100%), Positives = 50/50 (100%)
Frame = +3
Query: 66 ITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNI 115
ITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNI
Sbjct: 321 ITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNI 470
>AL375608 homologue to SP|Q9FE58|R22C_ 60S ribosomal protein L22-3.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (19%)
Length = 329
Score = 47.4 bits (111), Expect(2) = 4e-09
Identities = 28/49 (57%), Positives = 32/49 (65%), Gaps = 6/49 (12%)
Frame = +2
Query: 10 KGKKKGA-TFTIDCAKPVEDKIMD-----IASLEKFLQERIKVGGKAGA 52
+ KK+G TFTIDCAKPVEDKI + +ERIKVGGKAGA
Sbjct: 95 RAKKEGVXTFTIDCAKPVEDKIHGAFVAILVGXTFHNKERIKVGGKAGA 241
Score = 28.1 bits (61), Expect(2) = 4e-09
Identities = 12/14 (85%), Positives = 14/14 (99%)
Frame = +1
Query: 1 MSRGSAAGAKGKKK 14
MSRGSAAGAKGK++
Sbjct: 67 MSRGSAAGAKGKER 108
>TC92159 weakly similar to GP|19069568|emb|CAD25953. similarity to SKT5
PROTEIN {Encephalitozoon cuniculi}, partial (10%)
Length = 959
Score = 27.3 bits (59), Expect = 1.6
Identities = 14/52 (26%), Positives = 25/52 (47%), Gaps = 1/52 (1%)
Frame = +2
Query: 64 SKITVTSDSNFSKRY-LKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFN 114
++I ++ +F K++ L Y + + DWL + SI+ L YFN
Sbjct: 119 NRINEVNEKSFRKKHILDYFKNNNINSQEIYDWLLKHQYSSMNSIFLLGYFN 274
>TC78586 similar to PIR|T01041|T01041 hypothetical protein YUP8H12R.24 -
Arabidopsis thaliana, partial (38%)
Length = 2831
Score = 26.9 bits (58), Expect = 2.0
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +2
Query: 71 DSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNI 115
+ N KR+LK KK+ K HN + W+R+ K + I +++ N+
Sbjct: 521 ERNKGKRWLK---KKWKKHHNQQLWMRLKKILKLKRILKVKSVNL 646
>TC78448 similar to SP|Q42546|DPNP_ARATH 3'(2') 5'-bisphosphate nucleotidase
(EC 3.1.3.7) (3'(2') 5- bisphosphonucleoside 3'(2'),
partial (84%)
Length = 1545
Score = 26.2 bits (56), Expect = 3.5
Identities = 13/32 (40%), Positives = 15/32 (46%)
Frame = -2
Query: 80 KYLTKKYLKKHNVRDWLRVIASNKDRSIYELR 111
K+ +K KH LR I SN DRS R
Sbjct: 407 KWFRRKLSLKHQADQDLRTIISNSDRSFVRFR 312
>TC88548 similar to GP|20196934|gb|AAB86455.2 bZIP family transcription
factor {Arabidopsis thaliana}, partial (34%)
Length = 1570
Score = 26.2 bits (56), Expect = 3.5
Identities = 16/48 (33%), Positives = 25/48 (51%), Gaps = 1/48 (2%)
Frame = -1
Query: 69 TSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKD-RSIYELRYFNI 115
T+ SNF +L LT + WLR+ N++ R++ LR+ NI
Sbjct: 241 TNTSNFLSFHLTLLTLTLFTLRSRSSWLRLQPRNRNQRNLRTLRFNNI 98
>TC78116 similar to PIR|F86441|F86441 probable cytochrome P450 [imported] -
Arabidopsis thaliana, partial (80%)
Length = 1547
Score = 25.8 bits (55), Expect = 4.5
Identities = 13/52 (25%), Positives = 26/52 (50%)
Frame = -2
Query: 31 MDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKYL 82
M + S E+ + ++K + S+ + +S + VTS + +K+ KYL
Sbjct: 1030 MTLGSFERR*KVQVKTAAEVSCPAMSIVIKSSRSCLLVTSSPDANKKVKKYL 875
>TC81914 similar to GP|10178206|dbj|BAB11630. beta-glucosidase {Arabidopsis
thaliana}, partial (53%)
Length = 1259
Score = 25.8 bits (55), Expect = 4.5
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +3
Query: 7 AGAKGKKKGATFTIDCAKPVEDKIMDIASLE 37
A KGK++ TFTI C KIM LE
Sbjct: 807 ANKKGKEQ*FTFTIMCLTQYNKKIMQDQKLE 899
>TC78604 similar to PIR|E84456|E84456 probable acyl-CoA synthetase [imported]
- Arabidopsis thaliana, partial (91%)
Length = 2573
Score = 25.4 bits (54), Expect = 5.9
Identities = 16/57 (28%), Positives = 24/57 (42%)
Frame = +3
Query: 40 LQERIKVGGKAGALGDSVSVTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWL 96
L ++ G LGDS+ + KS I SNF+ + L + H V W+
Sbjct: 1755 LMRKVCAGFILVTLGDSILMGASKSSIGRRILSNFNMENISLLERLRQHSHQVIMWI 1925
>TC86000 phosphoinositide-specific phospholipase C [Medicago truncatula]
Length = 2160
Score = 25.0 bits (53), Expect = 7.7
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +3
Query: 11 GKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVSVTREKSKITVTS 70
GK KG ++C K DK+ ++ E L++ + GK ++ R K T +
Sbjct: 1143 GKPKGGI--VECLKVEPDKVRRLSLSESQLEKAAETYGKEIVRFTQQNILRVYPKGTRIT 1316
Query: 71 DSNFS 75
SN++
Sbjct: 1317 SSNYN 1331
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.312 0.131 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,673,084
Number of Sequences: 36976
Number of extensions: 25240
Number of successful extensions: 130
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of query: 124
length of database: 9,014,727
effective HSP length: 84
effective length of query: 40
effective length of database: 5,908,743
effective search space: 236349720
effective search space used: 236349720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144541.1