BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144540.4 - phase: 0
(1225 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91125 similar to PIR|T47659|T47659 spliceosomal-like protein -... 740 0.0
TC91942 similar to PIR|T47659|T47659 spliceosomal-like protein -... 246 e-107
AW684569 similar to PIR|T47659|T4 spliceosomal-like protein - Ar... 252 7e-67
TC90887 similar to PIR|T47659|T47659 spliceosomal-like protein -... 218 1e-56
BG453065 similar to PIR|T47659|T4 spliceosomal-like protein - Ar... 209 5e-54
AW694651 similar to PIR|T47659|T4 spliceosomal-like protein - Ar... 192 9e-49
AW683255 similar to PIR|T47659|T47 spliceosomal-like protein - A... 98 2e-20
TC89985 similar to GP|18377609|gb|AAL66955.1 putative UV-damaged... 61 2e-09
AW586820 39 0.016
AW692972 similar to PIR|B85068|B85 UV-damaged DNA binding factor... 38 0.021
BI271467 weakly similar to GP|22136758|gb unknown protein {Arabi... 32 1.5
TC77204 similar to GP|21536625|gb|AAM60957.1 RING-H2 zinc finger... 30 4.4
TC78230 similar to PIR|T10561|T10561 hypothetical protein F25E4.... 30 7.5
TC80458 similar to GP|21950739|gb|AAM78582.1 RanGAP1 interacting... 30 7.5
BQ751983 PIR|T29061|T29 probable integral membrane protein - Str... 29 9.8
BE942694 29 9.8
>TC91125 similar to PIR|T47659|T47659 spliceosomal-like protein - Arabidopsis
thaliana, partial (31%)
Length = 1157
Score = 740 bits (1910), Expect = 0.0
Identities = 352/385 (91%), Positives = 368/385 (95%)
Frame = +1
Query: 829 AHAGENKTGSEDQMENGGEDEDNDDSLSDEHYGYPKSESDKWVSCIRVLDPRTGNTTCLL 888
A AGEN T S DQMENGGEDED DD LSDEHYGYPK+E DKW SCIR+LDP+TGNTTCLL
Sbjct: 1 AQAGENGTESADQMENGGEDEDKDDPLSDEHYGYPKAELDKWASCIRILDPKTGNTTCLL 180
Query: 889 ELQENEAAFSICTVNFHDKEYGTLLAVGTAKGLQFTPKRSLTAGFIHIYRFLDDGRSLEL 948
ELQ+NEAAFS+CTVNFHDKEYGTLLAVGTAK LQFTP+RSLTAGFIHIYRFL+DGRSLEL
Sbjct: 181 ELQDNEAAFSVCTVNFHDKEYGTLLAVGTAKALQFTPRRSLTAGFIHIYRFLEDGRSLEL 360
Query: 949 LHKTQVEGVPLALCQFQGRLLAGIGPVLRLYDLGKRRLLRKCENKSFPSSIVSIHAYRDR 1008
LHKTQVEGVPLAL QFQGRLLAGIGPVLRLYDLGKRRLLRKCENK FP++IVSI YRDR
Sbjct: 361 LHKTQVEGVPLALSQFQGRLLAGIGPVLRLYDLGKRRLLRKCENKLFPNTIVSIQTYRDR 540
Query: 1009 IYVGGIQESFHYCKYRRDENQLYIFADDSVPRWLTSSYHIDFDTMAGADKFGNIFFARLP 1068
IYVG IQESFHYCKYRRDENQLYIFADD VPRWLT+SYHIDFDTMAGADKFGNI+F RLP
Sbjct: 541 IYVGDIQESFHYCKYRRDENQLYIFADDCVPRWLTASYHIDFDTMAGADKFGNIYFVRLP 720
Query: 1069 QDVSDEIEEDPTGGKIKWEQGKLNGAPNKVEEIVQFHVGDVITSLQKASLVPGGGECIVY 1128
QDVSDEIEEDPTGG+IKWEQGKLNGAPNKVEEIVQFHVGDVI+ LQKASL+PGGGECI+Y
Sbjct: 721 QDVSDEIEEDPTGGRIKWEQGKLNGAPNKVEEIVQFHVGDVISCLQKASLIPGGGECILY 900
Query: 1129 GTVMGSVGALHAFTSRDDVDFFSHLEMHMRQDNPPLCGRDHMAYRSAYFPVKDVIDGDLC 1188
GTVMGS+GALHAFTSRDDVDFFSHLEMHMRQDNPPLCGRDHMAYRSAYFPVKDVIDGDLC
Sbjct: 901 GTVMGSIGALHAFTSRDDVDFFSHLEMHMRQDNPPLCGRDHMAYRSAYFPVKDVIDGDLC 1080
Query: 1189 EQFPTLPMDLQRKIADELDRTPGEI 1213
EQFPTLPMDLQRKIADELDRTPGEI
Sbjct: 1081 EQFPTLPMDLQRKIADELDRTPGEI 1155
>TC91942 similar to PIR|T47659|T47659 spliceosomal-like protein -
Arabidopsis thaliana, partial (21%)
Length = 782
Score = 246 bits (627), Expect(4) = e-107
Identities = 117/134 (87%), Positives = 129/134 (95%)
Frame = +2
Query: 137 CEKKKLVYVLNRDSLARLTISSPLEANKSHTIVFSICAVDCGFENPIFAAIELDCSDADQ 196
CEK+KLVYVLNRD+ ARLTISSPLEA+KSHT+VFSIC VDCGFENPIFAAIELD S+AD
Sbjct: 2 CEKQKLVYVLNRDTAARLTISSPLEAHKSHTLVFSICGVDCGFENPIFAAIELDYSEADN 181
Query: 197 DATGVAASQAQKHLIFYELDLGLNHVSRKWSDQVDNGANMLVTVPGGADGPSGVLVCAEN 256
D++G+AA++AQK+L FYELDLGLNHVSRKWSDQVDNGAN+LVTVPGGADGPSGVLVCAEN
Sbjct: 182 DSSGMAAAEAQKNLTFYELDLGLNHVSRKWSDQVDNGANLLVTVPGGADGPSGVLVCAEN 361
Query: 257 FVIYKNQGHPDVRA 270
FVIYKNQGHPDVRA
Sbjct: 362 FVIYKNQGHPDVRA 403
Score = 93.6 bits (231), Expect(4) = e-107
Identities = 52/69 (75%), Positives = 54/69 (77%)
Frame = +3
Query: 271 VIPRRADLPAERGVLIVSAAMHKTKNLKPEEFKLFFLLQTEYGDIFKVTLTDGGGDRVSE 330
VIPRRADLPAERGVLIVSAAMHK K FFLLQTEYGDIFK TL + GDR+SE
Sbjct: 405 VIPRRADLPAERGVLIVSAAMHKLKG------GFFFLLQTEYGDIFKATL-EHEGDRISE 563
Query: 331 LNIKYFDTI 339
L IKYFDTI
Sbjct: 564 LKIKYFDTI 590
Score = 87.0 bits (214), Expect(4) = e-107
Identities = 45/53 (84%), Positives = 47/53 (87%)
Frame = +1
Query: 344 SICVLKSGFLFAASEFGNHALYQFKGIGDDDNDVGASSASLMETEEGFQPVFF 396
S+CVLKSGFLFAASEFGNH LYQFKGIGDDD DV ASSASLMETE+GFQ F
Sbjct: 604 SMCVLKSGFLFAASEFGNHGLYQFKGIGDDD-DV*ASSASLMETEKGFQLCVF 759
Score = 25.8 bits (55), Expect(4) = e-107
Identities = 10/13 (76%), Positives = 11/13 (83%)
Frame = +2
Query: 391 FQPVFFQPRRLKN 403
F VFFQPR+LKN
Sbjct: 743 FSSVFFQPRKLKN 781
>AW684569 similar to PIR|T47659|T4 spliceosomal-like protein - Arabidopsis
thaliana, partial (10%)
Length = 522
Score = 252 bits (643), Expect = 7e-67
Identities = 135/174 (77%), Positives = 144/174 (82%), Gaps = 2/174 (1%)
Frame = +2
Query: 588 RHEMSGDVACLDIAPVPKGRLRSRFLAVGSYDKTIRILSLDPDDCMQTLGIQSLSSAPES 647
+HEMSGDVACLDIAPVP+GR RSRFLAVGSYDKTIRILSLDPDDCMQTLGIQSLSSAPES
Sbjct: 2 KHEMSGDVACLDIAPVPEGRQRSRFLAVGSYDKTIRILSLDPDDCMQTLGIQSLSSAPES 181
Query: 648 LLFLEVQASVGGEDGADHPASLFLNAGLQNGVLSRTVVDMVTGLLSDTRSRFLGLKAPKL 707
LLFLEVQASVGGEDGADHPASLFLNAGLQNGVLSRTVVDMVTGLLSD+RSRFLGL+ PKL
Sbjct: 182 LLFLEVQASVGGEDGADHPASLFLNAGLQNGVLSRTVVDMVTGLLSDSRSRFLGLRPPKL 361
Query: 708 FPIIVRGKRAMLCLSSRPWLGYIHQGHFL--LTPLSYETLEYAASFSSDQCFEG 759
FPIIVRGKRAML P L ++H L TP + L F ++G
Sbjct: 362 FPIIVRGKRAMLLPVKSP-LAWLHSPRTLPFNTPFRXKPLNLLPHFXLTNVWKG 520
Score = 40.8 bits (94), Expect = 0.003
Identities = 18/22 (81%), Positives = 19/22 (85%)
Frame = +1
Query: 739 PLSYETLEYAASFSSDQCFEGV 760
PLSY TLE+AASF SDQC EGV
Sbjct: 457 PLSYXTLEFAASFXSDQCVEGV 522
>TC90887 similar to PIR|T47659|T47659 spliceosomal-like protein -
Arabidopsis thaliana, partial (13%)
Length = 710
Score = 218 bits (555), Expect = 1e-56
Identities = 110/110 (100%), Positives = 110/110 (100%)
Frame = +3
Query: 1 MYLYNLTLQRPTGIVCAINGNFSGSDDGITQEIVVARGKVLELLRPDKFGRIQSILSVQV 60
MYLYNLTLQRPTGIVCAINGNFSGSDDGITQEIVVARGKVLELLRPDKFGRIQSILSVQV
Sbjct: 150 MYLYNLTLQRPTGIVCAINGNFSGSDDGITQEIVVARGKVLELLRPDKFGRIQSILSVQV 329
Query: 61 FGTIRSLSQFRLTGAQKDFIVVGSDSGRIVILDYNKQKNVFDKIHQETFG 110
FGTIRSLSQFRLTGAQKDFIVVGSDSGRIVILDYNKQKNVFDKIHQETFG
Sbjct: 330 FGTIRSLSQFRLTGAQKDFIVVGSDSGRIVILDYNKQKNVFDKIHQETFG 479
Score = 143 bits (360), Expect = 5e-34
Identities = 71/79 (89%), Positives = 71/79 (89%)
Frame = +3
Query: 359 FGNHALYQFKGIGDDDNDVGASSASLMETEEGFQPVFFQPRRLKNLVRIDQVESLMPVMD 418
FGNHALYQFKGIGDDDNDVGASSASLMETE GFQPVFFQPRRLKNLVRIDQV SLMPVMD
Sbjct: 474 FGNHALYQFKGIGDDDNDVGASSASLMETEXGFQPVFFQPRRLKNLVRIDQVXSLMPVMD 653
Query: 419 MKVSNLFEEETPQIFTLCG 437
M VSNLFE ETPQI G
Sbjct: 654 MXVSNLFEXETPQILLFXG 710
>BG453065 similar to PIR|T47659|T4 spliceosomal-like protein - Arabidopsis
thaliana, partial (14%)
Length = 562
Score = 209 bits (532), Expect = 5e-54
Identities = 107/116 (92%), Positives = 109/116 (93%), Gaps = 4/116 (3%)
Frame = +2
Query: 26 DDGITQEIVVARGKVLELLRPDKFGRIQSILSVQVFGTIRSLSQFRLTGAQKDFIVVGSD 85
DDGITQEIVVARGKVLELLRPDKFGRIQSILSVQVFGTIRSLSQFRLTGAQKDFIVVGSD
Sbjct: 2 DDGITQEIVVARGKVLELLRPDKFGRIQSILSVQVFGTIRSLSQFRLTGAQKDFIVVGSD 181
Query: 86 SGRIVILDYNKQKNVFDKIHQETFGKSGCRRIVPGQYLAIDPKG----RAVMIAAC 137
SGRIVILDYNKQKNVFDKIHQETFGKSGCRRIVPGQYLAIDPKG R +M+ C
Sbjct: 182 SGRIVILDYNKQKNVFDKIHQETFGKSGCRRIVPGQYLAIDPKGSGLIRLIMVLIC 349
Score = 169 bits (427), Expect = 8e-42
Identities = 82/84 (97%), Positives = 82/84 (97%)
Frame = +1
Query: 224 RKWSDQVDNGANMLVTVPGGADGPSGVLVCAENFVIYKNQGHPDVRAVIPRRADLPAERG 283
RKWSDQVDNGANMLVTVPGGADGPSGVLVCAENFVIYKNQGHPDVRAVIPRRADLPAERG
Sbjct: 310 RKWSDQVDNGANMLVTVPGGADGPSGVLVCAENFVIYKNQGHPDVRAVIPRRADLPAERG 489
Query: 284 VLIVSAAMHKTKNLKPEEFKLFFL 307
VLIVSAAMHKT NLKPEEFKL FL
Sbjct: 490 VLIVSAAMHKTXNLKPEEFKLXFL 561
>AW694651 similar to PIR|T47659|T4 spliceosomal-like protein - Arabidopsis
thaliana, partial (11%)
Length = 503
Score = 192 bits (487), Expect = 9e-49
Identities = 98/113 (86%), Positives = 105/113 (92%)
Frame = +1
Query: 1 MYLYNLTLQRPTGIVCAINGNFSGSDDGITQEIVVARGKVLELLRPDKFGRIQSILSVQV 60
MYLYNLTLQRPTGI+CAINGNFSG G QEIVVARGK L+LLRPD GRIQ+ILSV+V
Sbjct: 100 MYLYNLTLQRPTGIICAINGNFSG---GKGQEIVVARGKTLDLLRPDDNGRIQTILSVEV 270
Query: 61 FGTIRSLSQFRLTGAQKDFIVVGSDSGRIVILDYNKQKNVFDKIHQETFGKSG 113
FG+IRSL+QFRLTGAQKDFIVVGSDSGRIVILDYNK+KNVFDKIHQETFGKSG
Sbjct: 271 FGSIRSLAQFRLTGAQKDFIVVGSDSGRIVILDYNKEKNVFDKIHQETFGKSG 429
Score = 50.1 bits (118), Expect = 5e-06
Identities = 23/42 (54%), Positives = 28/42 (65%)
Frame = +2
Query: 96 KQKNVFDKIHQETFGKSGCRRIVPGQYLAIDPKGRAVMIAAC 137
K+K K + G CRRIVPG+Y+A+ PKGRAVMI AC
Sbjct: 377 KRKMFLIKFIKRLLGNLVCRRIVPGEYIAVHPKGRAVMIGAC 502
>AW683255 similar to PIR|T47659|T47 spliceosomal-like protein - Arabidopsis
thaliana, partial (3%)
Length = 145
Score = 98.2 bits (243), Expect = 2e-20
Identities = 48/48 (100%), Positives = 48/48 (100%)
Frame = +2
Query: 801 KLLVVIESDQGALTAEEREAARKECFEAAHAGENKTGSEDQMENGGED 848
KLLVVIESDQGALTAEEREAARKECFEAAHAGENKTGSEDQMENGGED
Sbjct: 2 KLLVVIESDQGALTAEEREAARKECFEAAHAGENKTGSEDQMENGGED 145
>TC89985 similar to GP|18377609|gb|AAL66955.1 putative UV-damaged DNA binding
factor {Arabidopsis thaliana}, partial (88%)
Length = 975
Score = 61.2 bits (147), Expect = 2e-09
Identities = 57/251 (22%), Positives = 105/251 (41%), Gaps = 12/251 (4%)
Frame = +3
Query: 982 GKRRLLRKCENKSFPSSIVSIHAYRDRIYVGGIQESFHYCKYRRDENQLYIFADDSVPRW 1041
G R L +C + ++ + D I VG + +S Y+ +E + A D W
Sbjct: 18 GTRELQSECGHHGHILALY-VQTRGDFIVVGDLMKSISLLIYKHEEGAIEERARDYNANW 194
Query: 1042 LTSSYHIDFDTMAGADKFGNIFFARLPQDVSDEIEEDPTGGKIKWEQGKLNGAPNKVEEI 1101
+++ +D D GA+ N+F R K +G + ++E
Sbjct: 195 MSAVEILDDDVYLGAENSFNLFTVR------------------KNSEGATDEERGRLEVA 320
Query: 1102 VQFHVGDVITSLQKASLV-------PGGGECIVYGTVMGSVGALHAFTSRDDVDFFSHLE 1154
++H+G+ I + SLV G +++GT+ G +G + A + F L+
Sbjct: 321 GEYHLGEFINRFRHGSLVMRLPDSDVGQIPTVIFGTINGVIGVI-ASLPHEQYVFLEKLQ 497
Query: 1155 MHMRQDNPPLCGRDHMAYRSAYFPVKDV-----IDGDLCEQFPTLPMDLQRKIADELDRT 1209
++R+ + G H +RS K V +DGDL E F L +I+ ++ +
Sbjct: 498 SNLRKVIKGVGGLSHEQWRSFNNEKKTVEARNFLDGDLIESFLDLKRSKMDEISKAMEVS 677
Query: 1210 PGEILKKLEEV 1220
E+ K++EE+
Sbjct: 678 VEELAKRVEEL 710
>AW586820
Length = 479
Score = 38.5 bits (88), Expect = 0.016
Identities = 18/18 (100%), Positives = 18/18 (100%)
Frame = +2
Query: 1208 RTPGEILKKLEEVRNKII 1225
RTPGEILKKLEEVRNKII
Sbjct: 11 RTPGEILKKLEEVRNKII 64
>AW692972 similar to PIR|B85068|B85 UV-damaged DNA binding factor-like
protein [imported] - Arabidopsis thaliana, partial (7%)
Length = 482
Score = 38.1 bits (87), Expect = 0.021
Identities = 24/79 (30%), Positives = 42/79 (52%)
Frame = +2
Query: 4 YNLTLQRPTGIVCAINGNFSGSDDGITQEIVVARGKVLELLRPDKFGRIQSILSVQVFGT 63
Y +T +PT + + GNF+ D +++A+ +E+ G +QSIL V ++G
Sbjct: 212 YVVTAHKPTNVTHSCVGNFTSPQD---LNLILAKCTRIEIHLLTAQG-LQSILDVPLYGR 379
Query: 64 IRSLSQFRLTGAQKDFIVV 82
I +L FR G +DF+ +
Sbjct: 380 IATLELFRPHGETQDFLFI 436
>BI271467 weakly similar to GP|22136758|gb unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 478
Score = 32.0 bits (71), Expect = 1.5
Identities = 20/71 (28%), Positives = 36/71 (50%)
Frame = +3
Query: 1077 EDPTGGKIKWEQGKLNGAPNKVEEIVQFHVGDVITSLQKASLVPGGGECIVYGTVMGSVG 1136
E P+ ++KWE GK+ + K+ + + F + T A L+ G + +Y +GS
Sbjct: 6 EKPSLVELKWEVGKITLSTKKLPQRLPFTHSMMFTH-DSAWLILAGHDRRIYVVDVGSSK 182
Query: 1137 ALHAFTSRDDV 1147
+H FT R ++
Sbjct: 183 LVHTFTPRREL 215
>TC77204 similar to GP|21536625|gb|AAM60957.1 RING-H2 zinc finger
protein-like {Arabidopsis thaliana}, partial (30%)
Length = 1218
Score = 30.4 bits (67), Expect = 4.4
Identities = 16/51 (31%), Positives = 30/51 (58%)
Frame = -3
Query: 281 ERGVLIVSAAMHKTKNLKPEEFKLFFLLQTEYGDIFKVTLTDGGGDRVSEL 331
+R V + S ++ +T + + F + ++L +EY I KV++TDG R + L
Sbjct: 448 KRSVSVSSNSIKQTAHSRTS-FPILWILTSEYSRIGKVSITDGSSPRATAL 299
>TC78230 similar to PIR|T10561|T10561 hypothetical protein F25E4.30 -
Arabidopsis thaliana, partial (90%)
Length = 1121
Score = 29.6 bits (65), Expect = 7.5
Identities = 17/37 (45%), Positives = 24/37 (63%)
Frame = +2
Query: 731 HQGHFLLTPLSYETLEYAASFSSDQCFEGVVSVASEA 767
H GHFLLT L +T++ AS S + +V+V+SEA
Sbjct: 461 HLGHFLLTNLLLDTMKKTASESKTE--GRIVNVSSEA 565
>TC80458 similar to GP|21950739|gb|AAM78582.1 RanGAP1 interacting protein
{Arabidopsis thaliana}, partial (50%)
Length = 1536
Score = 29.6 bits (65), Expect = 7.5
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = +1
Query: 1026 DENQLYIFADDSVPRWLTSSYHIDFDTMA 1054
D+ LYIF S + L Y +DF+TMA
Sbjct: 163 DDKVLYIFGGSSKSKTLNDLYSLDFETMA 249
>BQ751983 PIR|T29061|T29 probable integral membrane protein - Streptomyces
coelicolor, partial (4%)
Length = 826
Score = 29.3 bits (64), Expect = 9.8
Identities = 13/42 (30%), Positives = 20/42 (46%)
Frame = +3
Query: 808 SDQGALTAEEREAARKECFEAAHAGENKTGSEDQMENGGEDE 849
S +L+ + + AR C + H N S D +EN ED+
Sbjct: 177 SPSASLSTRDGQLARARCLDRLHREPNTISSPDALENFTEDD 302
>BE942694
Length = 229
Score = 29.3 bits (64), Expect = 9.8
Identities = 11/17 (64%), Positives = 15/17 (87%)
Frame = +3
Query: 393 PVFFQPRRLKNLVRIDQ 409
PVFF P +LKNL+R+D+
Sbjct: 144 PVFFFPLKLKNLIRLDE 194
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.138 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,036,626
Number of Sequences: 36976
Number of extensions: 458182
Number of successful extensions: 2040
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1965
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2018
length of query: 1225
length of database: 9,014,727
effective HSP length: 107
effective length of query: 1118
effective length of database: 5,058,295
effective search space: 5655173810
effective search space used: 5655173810
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)
Medicago: description of AC144540.4