BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.7 - phase: 0
(261 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87668 similar to GP|14326469|gb|AAK60280.1 At1g32400/F5D14_22 ... 312 e-143
TC86548 similar to GP|14326469|gb|AAK60280.1 At1g32400/F5D14_22 ... 408 e-114
BF651057 weakly similar to GP|14326469|gb At1g32400/F5D14_22 {Ar... 170 6e-43
TC79470 similar to GP|15450980|gb|AAK96761.1 Unknown protein {Ar... 130 7e-31
TC84843 similar to GP|4567304|gb|AAD23715.1| unknown protein {Ar... 32 0.32
TC79263 similar to GP|6016736|gb|AAF01562.1| unknown protein {Ar... 32 0.32
TC77643 similar to GP|21105742|gb|AAM34770.1 nam-like protein 7 ... 31 0.42
TC90265 weakly similar to GP|9294666|dbj|BAB03015.1 gene_id:F14O... 31 0.55
TC84832 similar to GP|19697391|gb|AAL93029.1 IRE (root hair elon... 28 2.7
TC87117 similar to GP|8894548|emb|CAB95829.1 hypothetical protei... 28 2.7
TC82499 SP|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation f... 28 3.6
TC78482 weakly similar to GP|16209680|gb|AAL14398.1 At1g15230/F9... 28 3.6
TC91293 28 4.6
TC89658 weakly similar to PIR|A84527|A84527 hypothetical protein... 28 4.6
BQ135612 similar to GP|15028978|emb type II keratin E2 {Oncorhyn... 28 4.6
TC80490 similar to PIR|E84825|E84825 probable protein kinase [im... 27 6.1
TC80614 similar to GP|10140653|gb|AAG13489.1 hypothetical protei... 27 6.1
BG582085 27 7.9
>TC87668 similar to GP|14326469|gb|AAK60280.1 At1g32400/F5D14_22
{Arabidopsis thaliana}, partial (79%)
Length = 1553
Score = 312 bits (800), Expect(2) = e-143
Identities = 159/193 (82%), Positives = 161/193 (83%), Gaps = 28/193 (14%)
Frame = +2
Query: 1 MVCCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGD 60
MVCCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGD
Sbjct: 293 MVCCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGD 472
Query: 61 SALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCI----------- 109
SALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCI
Sbjct: 473 SALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIGATTRNGCCLI 652
Query: 110 -----------------AFLFFDKSWKEEIPTDKTGDFDMIYGFLRENWDIVRWVALGIV 152
AFLFFDKSWKEEIPTDKTGDFDMIYGFLRENWDIVRWVALGIV
Sbjct: 653 CYSILVALLILVELGCAAFLFFDKSWKEEIPTDKTGDFDMIYGFLRENWDIVRWVALGIV 832
Query: 153 IFEALLFLLALIV 165
IFEAL F L++
Sbjct: 833 IFEALSFC*PLLL 871
Score = 213 bits (541), Expect(2) = e-143
Identities = 103/103 (100%), Positives = 103/103 (100%)
Frame = +1
Query: 159 FLLALIVRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRNDA 218
FLLALIVRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRNDA
Sbjct: 850 FLLALIVRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRNDA 1029
Query: 219 WSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTEEKSRCTIM 261
WSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTEEKSRCTIM
Sbjct: 1030WSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTEEKSRCTIM 1158
>TC86548 similar to GP|14326469|gb|AAK60280.1 At1g32400/F5D14_22
{Arabidopsis thaliana}, partial (97%)
Length = 1396
Score = 408 bits (1048), Expect = e-114
Identities = 206/288 (71%), Positives = 223/288 (76%), Gaps = 28/288 (9%)
Frame = +1
Query: 2 VCCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDS 61
+ CRG WECLLKLLNFILT+TGLA+VGYGIYLLV F++ASD+ +S S D
Sbjct: 193 MACRGCWECLLKLLNFILTITGLAMVGYGIYLLVEFSRASDNSMM-------LSAPSDDQ 351
Query: 62 ALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIA----------- 110
L+Q GRPMLMA+ LSDNIFD LPKAWFIYLFIGVGAVLFV+SCFGCIA
Sbjct: 352 TLVQLGRPMLMALPLSDNIFDNLPKAWFIYLFIGVGAVLFVISCFGCIASMTRNGCCLSC 531
Query: 111 -----------------FLFFDKSWKEEIPTDKTGDFDMIYGFLRENWDIVRWVALGIVI 153
F+FFDKSWKEEIP DKTGDFDM+Y FLRENW IV+WVALGIVI
Sbjct: 532 YSILVVLLILVELGCAAFIFFDKSWKEEIPRDKTGDFDMVYNFLRENWTIVKWVALGIVI 711
Query: 154 FEALLFLLALIVRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRP 213
FEALLF+LALIVRA NRP DYDSD+E INPRQQ RQPLL NR AGP G+PV GTTD R
Sbjct: 712 FEALLFILALIVRAANRPVDYDSDEEFINPRQQARQPLL-NRPAGPAPGVPVTGTTDPRS 888
Query: 214 NRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQANSQPTEEKSRCTIM 261
NRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQ NSQP EE+SRCTIM
Sbjct: 889 NRNDAWSTRMREKYGLDTSEFTYNPSESQRFQQVNSQPAEERSRCTIM 1032
>BF651057 weakly similar to GP|14326469|gb At1g32400/F5D14_22 {Arabidopsis
thaliana}, partial (47%)
Length = 620
Score = 170 bits (430), Expect = 6e-43
Identities = 90/153 (58%), Positives = 101/153 (65%), Gaps = 28/153 (18%)
Frame = +1
Query: 2 VCCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDS 61
+ CRG WECLLKLLNFILT+TGLA+VGYGIYLLV F++ASD+ +S S D
Sbjct: 166 MACRGCWECLLKLLNFILTITGLAMVGYGIYLLVEFSRASDNSMM-------LSAPSDDQ 324
Query: 62 ALIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIA----------- 110
L+Q GRPMLMA+ LSDNIFD LPKAWFIYLFIGVGAVLFV+SCFGCIA
Sbjct: 325 TLVQLGRPMLMALPLSDNIFDNLPKAWFIYLFIGVGAVLFVISCFGCIASMTRNGCCLXC 504
Query: 111 -----------------FLFFDKSWKEEIPTDK 126
F+FFDKSWK EI DK
Sbjct: 505 YSILVVLLILXXLGCAAFIFFDKSWKXEIXRDK 603
>TC79470 similar to GP|15450980|gb|AAK96761.1 Unknown protein {Arabidopsis
thaliana}, partial (47%)
Length = 1041
Score = 130 bits (326), Expect = 7e-31
Identities = 87/261 (33%), Positives = 130/261 (49%), Gaps = 30/261 (11%)
Frame = +1
Query: 3 CCRGFWECLLKLLNFILTVTGLAIVGYGIYLLVAFAKASDDDKPDISPTPDISPVSGDSA 62
CC LLK LNF+ GL+I+ Y I++L + +P P + +S
Sbjct: 106 CCHFSLAFLLKFLNFLQAFVGLSILLYSIWML--------NQWDHFTPQPPLPHLS---- 249
Query: 63 LIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIA------------ 110
P + + +I LP WFIY F+ VG ++ ++ FGCIA
Sbjct: 250 ---LSLPRSFSPQFTSDI--NLPAPWFIYAFMCVGILVCCIAFFGCIAAEIINGCCLCFY 414
Query: 111 ----------------FLFFDKSWKEEIPTDKTGDFDMIYGFLRENWDIVRWVALGIVIF 154
F+ D+ W+E++P D TG I F+ +N DI RWV + +++
Sbjct: 415 TVLITVLVLLEAALVAFIAIDRQWQEDLPVDPTGQLASIRSFIEDNQDISRWVGIAVLVV 594
Query: 155 EALLFLLALIVRAV--NRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQR 212
+AL L+ALI+RA R AD+D D+E + R + R+PLLN+ Q+G SG+ D R
Sbjct: 595 QALSLLVALILRATVSTRRADFD-DEEEYDVRGRSREPLLNH-QSGQTSGI---SKGDIR 759
Query: 213 PNRNDAWSTRMREKYGLDTSE 233
N +D WS+RMREKYGL+ +
Sbjct: 760 GNLSDVWSSRMREKYGLNNGD 822
>TC84843 similar to GP|4567304|gb|AAD23715.1| unknown protein {Arabidopsis
thaliana}, partial (30%)
Length = 909
Score = 31.6 bits (70), Expect = 0.32
Identities = 16/74 (21%), Positives = 33/74 (43%)
Frame = +3
Query: 183 PRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRNDAWSTRMREKYGLDTSEFTYNPSESQ 242
P + P+++++ P+G G+ Q N WS + + +G+D + + E+
Sbjct: 318 PTDPISDPIVDSKVLPIPAGNLSFGSGAQLKNSVGNWSRLLTDMFGIDAEDCSEEYPENS 497
Query: 243 RFQQANSQPTEEKS 256
+ P E+KS
Sbjct: 498 ENDERRGGPGEQKS 539
>TC79263 similar to GP|6016736|gb|AAF01562.1| unknown protein {Arabidopsis
thaliana}, partial (58%)
Length = 1419
Score = 31.6 bits (70), Expect = 0.32
Identities = 21/71 (29%), Positives = 30/71 (41%)
Frame = +3
Query: 157 LLFLLALIVRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRN 216
+L L+ + VR V P YDS +Q QP + R PP T R R+
Sbjct: 213 ILTLIRVTVRVVIPPYTYDSQQPTYESQQPSYQPYYDARPPPPPQNYGYEPQTSGRVPRH 392
Query: 217 DAWSTRMREKY 227
D + ++ KY
Sbjct: 393 D-YQRKLDRKY 422
>TC77643 similar to GP|21105742|gb|AAM34770.1 nam-like protein 7 {Petunia x
hybrida}, partial (27%)
Length = 1611
Score = 31.2 bits (69), Expect = 0.42
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 109 IAFLFFDKSWKEEIPTD---KTGDFDMIYGFLRENWDIVRWVALGIVIFEALLFLLALIV 165
+ FLFFD + + IPT ++GF R+++D WV E F L + +
Sbjct: 410 LGFLFFDSTIRSSIPTI*GLNQSILLWVFGFDRKSFDFWCWV------IECCYFCLLIEM 571
Query: 166 RAVNRPADYDSDDELIN 182
AV+ A D E+++
Sbjct: 572 GAVSESASADCFSEVMS 622
>TC90265 weakly similar to GP|9294666|dbj|BAB03015.1
gene_id:F14O13.16~pir||T05437~similar to unknown protein
{Arabidopsis thaliana}, partial (6%)
Length = 1373
Score = 30.8 bits (68), Expect = 0.55
Identities = 22/84 (26%), Positives = 39/84 (46%)
Frame = +1
Query: 63 LIQFGRPMLMAVSLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIAFLFFDKSWKEEI 122
+I FGR +++ V L N+ W IY+ + +G ++ VS GC +W E++
Sbjct: 667 MIPFGRYIVLEVILGKNLISIC--TWVIYVVLFLGLIVLKVS--GC--------TWMEDV 810
Query: 123 PTDKTGDFDMIYGFLRENWDIVRW 146
G + L+E+W + W
Sbjct: 811 ----IGGI*LTILMLKEHWRRLTW 870
>TC84832 similar to GP|19697391|gb|AAL93029.1 IRE (root hair elongation)
{Dictyostelium discoideum}, partial (0%)
Length = 660
Score = 28.5 bits (62), Expect = 2.7
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -2
Query: 126 KTGDFDMIYGFLRENWDIVRWVAL 149
KT DF + G L+ NW+ RWV L
Sbjct: 125 KTWDFVTLMGVLKYNWNXERWVWL 54
>TC87117 similar to GP|8894548|emb|CAB95829.1 hypothetical protein {Cicer
arietinum}, partial (72%)
Length = 1662
Score = 28.5 bits (62), Expect = 2.7
Identities = 13/44 (29%), Positives = 20/44 (44%)
Frame = -3
Query: 75 SLSDNIFDKLPKAWFIYLFIGVGAVLFVVSCFGCIAFLFFDKSW 118
S D F L W+++ G A + VV CF + +K+W
Sbjct: 355 STEDTTFSFLSSNWYLFSCAGSAATVVVVDCFCSLMSGGAEKAW 224
>TC82499 SP|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation factor 3
subunit 8 (eIF3 p110) (eIF3c). [Barrel medic], partial
(91%)
Length = 2816
Score = 28.1 bits (61), Expect = 3.6
Identities = 23/70 (32%), Positives = 34/70 (47%), Gaps = 2/70 (2%)
Frame = +1
Query: 173 DYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRP-NRNDA-WSTRMREKYGLD 230
+Y+SDDE+I P Q+R+P P S + + RP + DA W ++ +K L
Sbjct: 808 EYESDDEMIEP-DQLRKP-------EPVSDSETSELGNDRPGDDGDAPWDQKLSKKDRLL 963
Query: 231 TSEFTYNPSE 240
F PSE
Sbjct: 964 EKMFMKKPSE 993
>TC78482 weakly similar to GP|16209680|gb|AAL14398.1 At1g15230/F9L1_18
{Arabidopsis thaliana}, partial (38%)
Length = 792
Score = 28.1 bits (61), Expect = 3.6
Identities = 17/45 (37%), Positives = 21/45 (45%), Gaps = 7/45 (15%)
Frame = +3
Query: 188 RQPLLNNRQAGPPSGLPVAGTTDQRPNRND-------AWSTRMRE 225
+QPLL NR+ P +A T RP R +W TR RE
Sbjct: 360 KQPLL*NREYSHPPTKMIASTIQSRPARMCPMSLV*LSWKTRSRE 494
>TC91293
Length = 772
Score = 27.7 bits (60), Expect = 4.6
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +1
Query: 1 MVCCRGFWECLLKL 14
M C RGFW CL++L
Sbjct: 418 MCCIRGFWSCLMQL 459
>TC89658 weakly similar to PIR|A84527|A84527 hypothetical protein At2g15280
[imported] - Arabidopsis thaliana, partial (59%)
Length = 1217
Score = 27.7 bits (60), Expect = 4.6
Identities = 24/91 (26%), Positives = 41/91 (44%), Gaps = 4/91 (4%)
Frame = +1
Query: 107 GCIAFLFFDKSWKEEIPTDKTGDFDMIYGFLRENWDIVRWVALGIVIFEALLFLLALIVR 166
G +A + K+W+ + T + Y F R ++ + +VA I++ +LFL A
Sbjct: 157 GLVADVLLWKNWRGGV-TLLISATTLWYLFERAGYNFLSFVANVILLLVVILFLWAKAAN 333
Query: 167 AVNRP----ADYDSDDELINPRQQVRQPLLN 193
+NRP D + ++ I V Q LN
Sbjct: 334 LLNRPLPPLPDLEISEKTIAKLSDVLQIWLN 426
>BQ135612 similar to GP|15028978|emb type II keratin E2 {Oncorhynchus
mykiss}, partial (8%)
Length = 938
Score = 27.7 bits (60), Expect = 4.6
Identities = 11/34 (32%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = -3
Query: 183 PRQQVRQPLLNNRQAG-PPSGLPVAGTTDQRPNR 215
P+ + P + + G PP G P++G + PNR
Sbjct: 555 PKCLITPPFIKGKHGGAPPGGFPISGGGKKTPNR 454
>TC80490 similar to PIR|E84825|E84825 probable protein kinase [imported] -
Arabidopsis thaliana, partial (7%)
Length = 798
Score = 27.3 bits (59), Expect = 6.1
Identities = 19/77 (24%), Positives = 37/77 (47%)
Frame = +1
Query: 165 VRAVNRPADYDSDDELINPRQQVRQPLLNNRQAGPPSGLPVAGTTDQRPNRNDAWSTRMR 224
+R+ +RP+++ S D + +N + + +A +T P+R+ S+
Sbjct: 457 LRSFSRPSEFTSGDGQFSK---------SNSVSSEEQFVSIASSTS--PSRSRVSSSEFI 603
Query: 225 EKYGLDTSEFTYNPSES 241
YG+++S T N SES
Sbjct: 604 NPYGINSSSQTQNDSES 654
>TC80614 similar to GP|10140653|gb|AAG13489.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (18%)
Length = 742
Score = 27.3 bits (59), Expect = 6.1
Identities = 12/22 (54%), Positives = 15/22 (67%)
Frame = +3
Query: 40 ASDDDKPDISPTPDISPVSGDS 61
+SD + IS TP+ SP SGDS
Sbjct: 222 SSDQNDHSISETPEYSPFSGDS 287
>BG582085
Length = 824
Score = 26.9 bits (58), Expect = 7.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 137 LRENWDIVRWVALGIVIFEALLFLL 161
+R WDI+ WV L IV+ L LL
Sbjct: 484 VRHTWDIIVWVLLIIVLVHFFLTLL 410
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.141 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,715,951
Number of Sequences: 36976
Number of extensions: 124187
Number of successful extensions: 969
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 956
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 961
length of query: 261
length of database: 9,014,727
effective HSP length: 94
effective length of query: 167
effective length of database: 5,538,983
effective search space: 925010161
effective search space used: 925010161
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144516.7