BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144513.14 + phase: 0
(593 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP212791 NP212791|AJ249611.1|CAB65291.1 pectin methyl-esterase PEF1 456 e-128
TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Cit... 366 e-101
TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precurs... 360 1e-99
TC76734 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11... 346 2e-95
TC76732 homologue to PIR|T06468|T06468 pectinesterase (EC 3.1.1.... 317 1e-86
TC76733 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11... 315 2e-86
TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 ... 308 3e-84
TC77735 similar to GP|14334646|gb|AAK59501.1 putative pectineste... 304 5e-83
TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylestera... 291 6e-79
TC86403 similar to PIR|B86158|B86158 hypothetical protein AAF028... 287 7e-78
TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectineste... 236 2e-62
TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor ... 227 9e-60
BE319906 similar to SP|Q43062|PME_ Pectinesterase PPE8B precurso... 182 3e-50
BI272853 weakly similar to PIR|T04359|T04 pectin methylesterase-... 193 1e-49
BQ138825 similar to PIR|T49922|T49 pectin methylesterase-like pr... 174 7e-44
TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pec... 136 8e-41
AL368659 similar to PIR|B86158|B86 hypothetical protein AAF02886... 148 5e-36
TC89457 weakly similar to SP|Q43062|PME_PRUPE Pectinesterase PPE... 108 6e-24
BI271313 similar to GP|6688852|emb pectin methyl-esterase PEF1 {... 94 5e-23
TC92167 similar to PIR|T05203|T05203 pectinesterase homolog F4I1... 91 1e-18
>NP212791 NP212791|AJ249611.1|CAB65291.1 pectin methyl-esterase PEF1
Length = 1698
Score = 456 bits (1173), Expect = e-128
Identities = 250/587 (42%), Positives = 347/587 (58%), Gaps = 9/587 (1%)
Frame = +1
Query: 5 GDAKRNKRLAIIGVSTFLLVAMVVAVTVNVGFNNKKEPGEETSKESHVSQSVKAVKTLCA 64
G + K+ A++GVS LLVAMV V V++ G + +++H+S S K V LC
Sbjct: 22 GGQGQGKKHALLGVSCILLVAMVGVVAVSL------TKGGDGEQKAHISNSQKNVDMLCQ 183
Query: 65 PTDYKKECEDSLIAHAGNITEPKELIKIAFNITIAKISEGLKKTHLLQEAEKDERTKQAL 124
T +K+ C +L + + K IK A T ++ + + + L QE D TKQA+
Sbjct: 184 STKFKETCHKTL--EKASFSNMKNRIKGALGATEEELRKHINNSALYQELATDSMTKQAM 357
Query: 125 DTCKQVMQLSIDEFQRSLERFSNFDLNSLDRVLTSLKVWLSGAITYQETCLDAFENTTTD 184
+ C +V+ ++D +S+ FD + L +KVWL+G +++Q+TCLD F NT T
Sbjct: 358 EICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDIKVWLTGTLSHQQTCLDGFVNTKTH 537
Query: 185 AGKKMKEVLQTSMHMSSNGLS---IINQLSKTFEEMKQPAGRRLLKESVDGEEDVLGHGG 241
AG+ M +VL+TSM +SSN + +++++ K F + RRLL + DG
Sbjct: 538 AGETMAKVLKTSMELSSNAIDMMDVVSRILKGFHPSQYGVSRRLLSD--DG--------- 684
Query: 242 DFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFTTITEALKHVPKKNLKPFVI 301
+P WV D G R LL G ++A+ VVA+DGSG F T+T+ALK VP N PFVI
Sbjct: 685 ---IPSWVSD--GHRHLL---AGGNVKANAVVAQDGSGQFKTLTDALKTVPPTNAAPFVI 840
Query: 302 YIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTASVAITGDFFVGI 361
Y+K GVYKE V V K M +V IGDG KT+ TG+ N+ DG+ T+KTA+ + G F+
Sbjct: 841 YVKAGVYKETVNVAKEMNYVTVIGDGPTKTKFTGSLNYADGINTYKTATFGVNGANFMAK 1020
Query: 362 GIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCIISGTIDFV 421
IGFEN+AG K QAVALRV +D++IF+ C+MDG+QDTL+ + RQFYRDC ISGTIDFV
Sbjct: 1021DIGFENTAGTSKFQAVALRVTADQAIFHNCQMDGFQDTLFVESQRQFYRDCAISGTIDFV 1200
Query: 422 FGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVRLKN 481
FGD+ V QNC + R P + Q+C+VTA GR ++N + L+ +P V K
Sbjct: 1201FGDAFGVFQNCKLICRVPAKGQKCLVTAGGRDKQNSASALVFLSSHFTGEPALTSVTPK- 1377
Query: 482 KAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGITGTDTCYYGEYNNRGPGSDVK 541
+YL RPWK +S+ + +D+ I M PEGYMP G DTC + EYNN+GPG+D
Sbjct: 1378LSYLGRPWKLYSKVVIMDSTIDAMFAPEGYMPM---VGGAFKDTCTFYEYNNKGPGADTN 1548
Query: 542 QRVKWQGVKTITSEGAASFVPIRFFH------GDDWIRVTRVPYSPG 582
RVKW GVK +TS AA + P +FF D WI + VPYS G
Sbjct: 1549LRVKWHGVKVLTSNVAAEYYPGKFFEIVNATARDTWIVKSGVPYSLG 1689
>TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Citrus
sinensis}, partial (85%)
Length = 1993
Score = 366 bits (939), Expect = e-101
Identities = 229/600 (38%), Positives = 326/600 (54%), Gaps = 18/600 (3%)
Frame = +1
Query: 1 MSGGGDAKRNKRLAIIGVSTFLLVAMVVAVTVNVGFNNKKEPGEETSKES----HVSQSV 56
++G ++ K+L I +T L+VA +VA+ N+ K + S H S ++
Sbjct: 70 LAGISNSGNKKKLLISLFTTLLIVASIVAIVATTTKNSNKSKNNSIASSSLSLSHHSHAI 249
Query: 57 KAVKTLCAPTDYKKECEDSLIAHAG---NITEPKELIKIAFNITIAKISEGLKKTH-LLQ 112
+K+ C T Y + C ++ + IT K++I ++ NIT + LL
Sbjct: 250 --LKSACTTTLYPELCFSAISSEPNITHKITNHKDVISLSLNITTRAVEHNYFTVEKLLL 423
Query: 113 EAEKDERTKQALDTCKQVMQLSIDEFQRSLERFSNF-DLNSLDRVLTSLKVWLSGAITYQ 171
+R K AL C + + ++DE + + + +L + LK +S AIT Q
Sbjct: 424 RKSLTKREKIALHDCLETIDETLDELKEAQNDLVLYPSKKTLYQHADDLKTLISSAITNQ 603
Query: 172 ETCLDAFENTTTDAGKKMKEVLQTSM----HMSSNGLSIINQLSKT----FEEMKQPAGR 223
TCLD F + DA K++++VLQ HM SN L++ ++ FE+ G
Sbjct: 604 VTCLDGFSHD--DADKEVRKVLQEGQIHVEHMCSNALAMTKNMTDKDIAEFEQTNMVLGS 777
Query: 224 RLLKESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFTT 283
++ ++ E V PEW+ AG R+LL G ++A VVVA DGSGNF T
Sbjct: 778 NKNRKLLEEENGV-------GWPEWIS--AGDRRLLQ---GSTVKADVVVAADGSGNFKT 921
Query: 284 ITEALKHVPKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGV 343
++EA+ P K+ K +VI IK GVYKE VEV K T+++F+GDG T ITG++N +DG
Sbjct: 922 VSEAVAAAPLKSSKRYVIKIKAGVYKENVEVPKKKTNIMFLGDGRTNTIITGSRNVVDGS 1101
Query: 344 GTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAH 403
TF +A+VAI G F+ I F+N+AGP KHQAVALRV +D S FY C + YQDTLY H
Sbjct: 1102TTFHSATVAIVGGNFLARDITFQNTAGPAKHQAVALRVGADLSAFYNCDIIAYQDTLYVH 1281
Query: 404 TMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLII 463
RQF+ +C ISGT+DF+FG+S V QNC R+P Q+ +VTAQGR + NQ TG++I
Sbjct: 1282NNRQFFVNCFISGTVDFIFGNSAVVFQNCDIHARRPNSGQKNMVTAQGRVDPNQNTGIVI 1461
Query: 464 QGGSIVADPKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGITGT 523
Q I A V+ YL RPWK++SRT+F+ + I D+I P G+ W G
Sbjct: 1462QKCRIGATKDLEGVKGNFPTYLGRPWKEYSRTVFMQSSISDVIDPVGWHEWN---GNFAL 1632
Query: 524 DTCYYGEYNNRGPGSDVKQRVKWQGVKTITSEG-AASFVPIRFFHGDDWIRVTRVPYSPG 582
+T Y EY N GPG+ +RV W+G K ITS A S P F G W+ T P+S G
Sbjct: 1633NTLVYREYQNTGPGAGTSKRVTWKGFKVITSAAEAQSSTPGNFIGGSSWLGSTGFPFSLG 1812
>TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precursor (EC
3.1.1.11) (Pectin methylesterase 3) (PE 3)., partial
(93%)
Length = 2088
Score = 360 bits (923), Expect = 1e-99
Identities = 209/580 (36%), Positives = 321/580 (55%), Gaps = 8/580 (1%)
Frame = +2
Query: 8 KRNKRLAIIGVSTFLLVAMVVAVTVNVGFN--NKKEPGEETSKESHVSQSVKAVKTLCAP 65
K KR+ II +S+ +LVA+++A + + N + S + ++K +C
Sbjct: 128 KTRKRITIIIISSIILVAVIIAAVAGILIHKHNTESSSSPNSLPNTELTPATSLKAVCES 307
Query: 66 TDYKKECEDSLIAHA-GNITEPKELIKIAFNITIAKISEGLKKTHLLQEAEKDERTKQAL 124
T Y C S+ + N T+P++L K++ + I ++S+ L T ++A + R K+A+
Sbjct: 308 TQYPNSCFSSISSLPDSNTTDPEQLFKLSLKVAIDELSK-LSLTRFSEKAT-EPRVKKAI 481
Query: 125 DTCKQVMQLSIDEFQRSLERFSNFDLNSLDRVLTSLKVWLSGAITYQETCLDAFENTTTD 184
C V+ S+D S+ + + ++ WLS A+T +TCLDA +
Sbjct: 482 GVCDNVLADSLDRLNDSMSTIVDGGKMLSPAKIRDVETWLSAALTDHDTCLDAVGEVNST 661
Query: 185 AGK----KMKEVLQTSMHMSSNGLSIINQLSKTFEEMKQPAGRRLLKESVDGEEDVLGHG 240
A + +++ +++ S +SN L+I++++ + + R L LG
Sbjct: 662 AARGVIPEIERIMRNSTEFASNSLAIVSKVIRLLSNFEVSNHHRRL----------LG-- 805
Query: 241 GDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFTTITEALKHVPKKNLKPFV 300
E PEW+ R+LL + + VVAKDGSG + TI EALK V KK+L+ FV
Sbjct: 806 ---EFPEWLG--TAERRLLATVVNETVP-DAVVAKDGSGQYKTIGEALKLVKKKSLQRFV 967
Query: 301 IYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTASVAITGDFFVG 360
+Y+K+GVY E +++ K +V+ GDG +T ++G++N+IDG TF+TA+ A+ G F+
Sbjct: 968 VYVKKGVYVENIDLDKNTWNVMIYGDGMTETVVSGSRNYIDGTPTFETATFAVKGKGFIA 1147
Query: 361 IGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCIISGTIDF 420
I F N+AG KHQAVA+R SD+S+FY+C GYQDTLYAH+ RQFYRDC I+GTIDF
Sbjct: 1148KDIQFLNTAGASKHQAVAMRSGSDQSVFYRCSFVGYQDTLYAHSNRQFYRDCDITGTIDF 1327
Query: 421 VFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVRLK 480
+FG++ AV QNC + R+P+ NQ +TAQG+K+ NQ +G++IQ + P L
Sbjct: 1328IFGNAAAVFQNCKIMPRQPMSNQFNTITAQGKKDPNQNSGIVIQKSTFTTLP---GDNLI 1498
Query: 481 NKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGITGTDTCYYGEYNNRGPGSDV 540
YL RPWKDFS TI + + IG + P G++ W A + + Y EY N GPG+DV
Sbjct: 1499APTYLGRPWKDFSTTIIMKSEIGSFLKPVGWISW--VANVEPPSSILYAEYQNTGPGADV 1672
Query: 541 KQRVKWQGVK-TITSEGAASFVPIRFFHGDDWIRVTRVPY 579
RVKW G K + E A F F G +W+ V +
Sbjct: 1673PGRVKWAGYKPALGDEDAIKFTVDSFIQGPEWLPSASVQF 1792
>TC76734 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11) precursor
- garden pea, complete
Length = 1981
Score = 346 bits (887), Expect = 2e-95
Identities = 192/488 (39%), Positives = 280/488 (57%), Gaps = 2/488 (0%)
Frame = +3
Query: 101 ISEGLKKTHLLQEAEKDERTKQALDTCKQVMQLSIDEFQRSLERFSNFDLNSLDRVLTSL 160
I + + ++++ R + AL+ C+++M LS++ S+ + +++S T
Sbjct: 336 IRKAMDTANVIKRRINSPREENALNVCEKLMNLSMERVWDSVLTLTKDNMDSQQDAHT-- 509
Query: 161 KVWLSGAITYQETCLDAFENTTTDAGKKMKEVLQTSMHMSSNGLSIINQL--SKTFEEMK 218
WLS +T TCLD E T+ M+ +Q + + + L+++ + K +E
Sbjct: 510 --WLSSVLTNHATCLDGLEGTSRAV---MENDIQDLIARARSSLAVLVAVLPPKDHDEF- 671
Query: 219 QPAGRRLLKESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGS 278
+ ES++G+ P WV + R+LL G ++A+VVVAKDGS
Sbjct: 672 -------IDESLNGD-----------FPSWVTSKD--RRLLESSVG-DVKANVVVAKDGS 788
Query: 279 GNFTTITEALKHVPKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKN 338
G F T+ EA+ P K +VIY+K+G+YKE VE+ + T+V+ +GDG T ITG+ N
Sbjct: 789 GKFKTVAEAVASAPNKGTARYVIYVKKGIYKENVEIASSKTNVMLLGDGMDATIITGSLN 968
Query: 339 FIDGVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQD 398
++DG GTF+TA+VA GD+F+ IGF+N+AGP+KHQAVALRV SDRS+ +C++D +QD
Sbjct: 969 YVDGTGTFQTATVAAVGDWFIAQDIGFQNTAGPQKHQAVALRVGSDRSVINRCKIDAFQD 1148
Query: 399 TLYAHTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQP 458
TLYAHT RQFYRD I+GTIDF+FGD+ VLQ C V RKP+ NQ +VTAQGR + NQ
Sbjct: 1149TLYAHTNRQFYRDSFITGTIDFIFGDAAVVLQKCKLVARKPMANQNNMVTAQGRIDPNQN 1328
Query: 459 TGLIIQGGSIVADPKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPA 518
T IQ ++ PV K YL RPWK +SRT+ + + +G I P G+ W A
Sbjct: 1329TATSIQQCDVIPSTDLKPVIGSVKTYLGRPWKKYSRTVVMQSLLGAHIDPTGWAEWDA-A 1505
Query: 519 GITGTDTCYYGEYNNRGPGSDVKQRVKWQGVKTITSEGAASFVPIRFFHGDDWIRVTRVP 578
T YYGEY N GPG+ +RVKW G I + A F + G+ W++ T V
Sbjct: 1506SKDFLQTLYYGEYMNSGPGAGTSKRVKWPGYHIINTAEANKFTVAQLIQGNVWLKNTGVA 1685
Query: 579 YSPGATKT 586
+ G K+
Sbjct: 1686FIAGL*KS 1709
>TC76732 homologue to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11)
precursor - garden pea, complete
Length = 1860
Score = 317 bits (811), Expect = 1e-86
Identities = 182/491 (37%), Positives = 272/491 (55%), Gaps = 2/491 (0%)
Frame = +2
Query: 99 AKISEGLKKTHLLQEAEKDERTKQALDTCKQVMQLSIDEFQRSLERFSNFDLNSLDRVLT 158
A I + + ++++ R + AL+ C+++M LS+D S+ + +++S T
Sbjct: 323 AHIRKAMDTANVIKRRVNSPREEIALNDCEELMDLSMDRVWDSVLTLTKNNIDSQHDAHT 502
Query: 159 SLKVWLSGAITYQETCLDAFENTTTDAGKKMKEVLQTSMH-MSSNGLSIINQLSKTFEEM 217
WLS +T TCLD E ++ + V+++ +H + S S L+ +
Sbjct: 503 ----WLSSVLTNHATCLDGLEGSS-------RVVMESDLHDLISRARS---SLAVLVSVL 640
Query: 218 KQPAGRRLLKESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDG 277
A + E ++G+ P WV + R+LL G ++A+VVVA+DG
Sbjct: 641 PPKANDGFIDEKLNGD-----------FPSWVTSKD--RRLLESSVG-DIKANVVVAQDG 778
Query: 278 SGNFTTITEALKHVPKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNK 337
SG F T+ +A+ P +VIY+K+G YKE +E+ K T+V+ +GDG T ITG+
Sbjct: 779 SGKFKTVAQAVASAPDNGKTRYVIYVKKGTYKENIEIGKKKTNVMLVGDGMDATIITGSL 958
Query: 338 NFIDGVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQ 397
NFIDG TFK+A+VA GD F+ I F+N+AGP+KHQAVALRV +D+S+ +C++D +Q
Sbjct: 959 NFIDGTTTFKSATVAAVGDGFIAQDIRFQNTAGPQKHQAVALRVGADQSVINRCKIDAFQ 1138
Query: 398 DTLYAHTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQ 457
DTLYAH+ RQFYRD I+GT+DF+FG++ V Q RKP+ NQ+ +VTAQGR++ NQ
Sbjct: 1139DTLYAHSNRQFYRDSYITGTVDFIFGNAAVVFQKSKLAARKPMANQKNMVTAQGREDPNQ 1318
Query: 458 PTGLIIQGGSIVADPKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTP 517
T IQ ++ PV+ K YL RPWK +SRT+ L + + I P G+ W
Sbjct: 1319NTATSIQQCDVIPSSDLKPVQGSIKTYLGRPWKKYSRTVVLQSVVDGHIDPAGWAEWDA- 1495
Query: 518 AGITGTDTCYYGEYNNRGPGSDVKQRVKWQGVKTITSEGAAS-FVPIRFFHGDDWIRVTR 576
A T YYGEY N G G+ +RV W G I + AS F + G+ W++ T
Sbjct: 1496ASKDFLQTLYYGEYMNSGAGAGTGKRVTWPGYHIIKNAAEASKFTVTQLIQGNVWLKNTG 1675
Query: 577 VPYSPGATKTP 587
V + G K P
Sbjct: 1676VAFIEGL*KCP 1708
>TC76733 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11) precursor
- garden pea, partial (98%)
Length = 2029
Score = 315 bits (808), Expect = 2e-86
Identities = 181/483 (37%), Positives = 265/483 (54%), Gaps = 1/483 (0%)
Frame = +2
Query: 101 ISEGLKKTHLLQEAEKDERTKQALDTCKQVMQLSIDEFQRSLERFSNFDLNSLDRVLTSL 160
I + +++ R + AL C+Q+M LS++ ++ + + +++S T
Sbjct: 344 IQNAMDTASVIKRRINSPREEIALSDCEQLMDLSMNRIWDTMLKLTKNNIDSQQDAHT-- 517
Query: 161 KVWLSGAITYQETCLDAFENTTTDAGKKMKEVLQTSMHMSSNGLSIINQLSKTFEEMKQP 220
WLS +T TCLD E ++ M+ LQ + + + L++ + Q
Sbjct: 518 --WLSSVLTNHATCLDGLEGSSRVV---MENDLQDLISRARSSLAVFLVV------FPQK 664
Query: 221 AGRRLLKESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGN 280
+ + E++ GE P WV + R+LL G ++A+VVVA+DGSG
Sbjct: 665 DRDQFIDETLIGE-----------FPSWVTSKD--RRLLETAVG-DIKANVVVAQDGSGK 802
Query: 281 FTTITEALKHVPKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFI 340
F T+ EA+ P +VIY+K+G YKE VE+ T+V+ +GDG T ITGN NFI
Sbjct: 803 FKTVAEAVASAPDNGKTKYVIYVKKGTYKENVEIGSKKTNVMLVGDGMDATIITGNLNFI 982
Query: 341 DGVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTL 400
DG TFK+++VA GD F+ I F+N AG KHQAVALRV SD+S+ +CR+D +QDTL
Sbjct: 983 DGTTTFKSSTVAAVGDGFIAQDIWFQNMAGAAKHQAVALRVGSDQSVINRCRIDAFQDTL 1162
Query: 401 YAHTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTG 460
YAH+ RQFYRD +I+GTIDF+FG++ V Q C V RKP+ NQ + TAQGR++ Q TG
Sbjct: 1163YAHSNRQFYRDSVITGTIDFIFGNAAVVFQKCKLVARKPMANQNNMFTAQGREDPGQNTG 1342
Query: 461 LIIQGGSIVADPKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGI 520
IQ + PV K +L RPWK +SRT+ + +++ I P G+ W A
Sbjct: 1343TSIQQCDLTPSSDLKPVVGSIKTFLGRPWKKYSRTVVMQSFLDSHIDPTGWAEWDA-ASK 1519
Query: 521 TGTDTCYYGEYNNRGPGSDVKQRVKWQGVKTI-TSEGAASFVPIRFFHGDDWIRVTRVPY 579
T YYGEY N GPG+ +RV W G I T+ A+ F + G+ W++ T V +
Sbjct: 1520DFLQTLYYGEYLNNGPGAGTAKRVTWPGYHVINTAAEASKFTVAQLIQGNVWLKNTGVAF 1699
Query: 580 SPG 582
+ G
Sbjct: 1700TEG 1708
>TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 {Arabidopsis
thaliana}, partial (79%)
Length = 2442
Score = 308 bits (790), Expect = 3e-84
Identities = 196/532 (36%), Positives = 279/532 (51%), Gaps = 4/532 (0%)
Frame = +3
Query: 55 SVKAVKTLCAPTDYKKECEDSLIAHAGN--ITEPKELIKIAFNITIAKISEGLKKTHLLQ 112
S+ ++K+ C+ T Y + CE L +A N I + K++ I + + +G + TH +
Sbjct: 639 SLSSIKSWCSQTPYPQPCEYYLTNNAFNQTIKSKSDFFKVSLEIAMERAKKGEENTHAVG 818
Query: 113 EAEKDERTKQALDTCKQVMQLSIDEF-QRSLERFSNFDLNSLDRVLTSLKVWLSGAITYQ 171
+ + K A C ++ ++ + Q + + ++ + WLS A+T
Sbjct: 819 PKCRSPQEKAAWADCLELYDFTVQKLSQTKYTKCTQYESQT----------WLSTALTNL 968
Query: 172 ETCLDAFENTTTDAGKKMKEVLQTSMHMSSNGLSIINQLSKTFEEMKQPAGRRLLKESVD 231
ETC + F D G V + + SN ++ + LS T K P + K+
Sbjct: 969 ETCKNGFY----DLG-----VTNYVLPLLSNNVTKL--LSNTLSLNKVPYQQPSYKDG-- 1109
Query: 232 GEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFTTITEALKHV 291
P WV + G RKLL + +A+VVVAKDGSG +TT+ A
Sbjct: 1110 -------------FPTWV--KPGDRKLLQTSSAAS-KANVVVAKDGSGKYTTVKAATDAA 1241
Query: 292 PKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTASV 351
P + + +VIY+K GVY E VE+ +V+ +GDG KT ITG+K+ G TF++A+V
Sbjct: 1242 PSGSGR-YVIYVKAGVYNEQVEIKAK--NVMLVGDGIGKTIITGSKSVGGGTTTFRSATV 1412
Query: 352 AITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRD 411
A TGD F+ I F N+AG HQAVA R SD S+FYKC +GYQDTLY H+ RQFYR+
Sbjct: 1413 AATGDGFIAQDITFRNTAGAANHQAVAFRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRE 1592
Query: 412 CIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVAD 471
C I GT+DF+FG++ VLQNC R P + VTAQGR + NQ TG+II + A
Sbjct: 1593 CNIYGTVDFIFGNAAVVLQNCNIFARNP-PAKTITVTAQGRTDPNQNTGIIIHNSRVSAQ 1769
Query: 472 PKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGITGTDTCYYGEY 531
P + K+YL RPW+ +SRT+F+ T + I P G++PW G DT YY EY
Sbjct: 1770 SDLNPSSV--KSYLGRPWQKYSRTVFMKTVLDGFINPAGWLPWD---GNFALDTLYYAEY 1934
Query: 532 NNRGPGSDVKQRVKWQGVKTITSEGAAS-FVPIRFFHGDDWIRVTRVPYSPG 582
N G GS RV W+G +TS AS F F G+ WI T VP++ G
Sbjct: 1935 ANTGSGSSTSNRVTWKGYHVLTSASQASPFTVGNFIAGNSWIGNTGVPFTSG 2090
>TC77735 similar to GP|14334646|gb|AAK59501.1 putative pectinesterase
{Arabidopsis thaliana}, partial (59%)
Length = 1963
Score = 304 bits (779), Expect = 5e-83
Identities = 184/541 (34%), Positives = 283/541 (52%), Gaps = 21/541 (3%)
Frame = +2
Query: 63 CAPTDYKKECEDSLIAHAGNITEPKELIKIAFNITIAKISEGLKKTHL------LQEAEK 116
C T Y K C L A + ++P K + + K++ L+K + +
Sbjct: 155 CKTTLYPKLCRSMLSAIRSSPSDPYNYGKFSIKQNL-KVARKLEKVFIDFLNRHQSSSSL 331
Query: 117 DERTKQALDTCKQVMQLSIDEFQR---SLERFSNFDLNSLDRVLTSLKVWLSGAITYQET 173
+ AL CK + L++D + L+ S+ +S ++ ++ +LS T T
Sbjct: 332 NHEEVGALVDCKDLNSLNVDYLESISDELKSASSSSSSSDTELVDKIESYLSAVATNHYT 511
Query: 174 CLDAFENTTTDAGKKMKEVLQTSMHMSSNGLSIINQ-LSKTFEEMKQ-----PAGRRLLK 227
C D T ++ + L+ + S L ++ + LSK + K P ++
Sbjct: 512 CYDGLVVTKSNIANALAVPLKDATQFYSVSLGLVTEALSKNMKRNKTRKHGLPNKSFKVR 691
Query: 228 ESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMT--GRKLQAHVVVAKDGSGNFTTIT 285
+ ++ +L + ++L + G L V+V+ G N T+I
Sbjct: 692 QPLEKLIKLLRTKYSCQKTSSNCTSTRTERILKESESHGILLNDFVLVSPYGIANHTSIG 871
Query: 286 EALKHVPKKNLKP----FVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFID 341
+A+ P N KP ++IY++EG Y+EYV V K +++ +GDG T ITGN + ID
Sbjct: 872 DAIAAAPN-NTKPEDGYYLIYVREGYYEEYVIVPKHKNNILLVGDGINNTIITGNHSVID 1048
Query: 342 GVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLY 401
G TF +++ A++G+ F+ + I F N+AGPEKHQAVA+R +D S FY+C +GYQDTLY
Sbjct: 1049GWTTFNSSTFAVSGERFIAVDITFRNTAGPEKHQAVAVRNNADLSTFYRCSFEGYQDTLY 1228
Query: 402 AHTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGL 461
H++RQFYRDC I GT+DF+FG++ V QNC RKPL NQ+ VTAQGR + NQ TG+
Sbjct: 1229VHSVRQFYRDCKIYGTVDFIFGNAAVVFQNCNIYARKPLPNQKNAVTAQGRTDPNQNTGI 1408
Query: 462 IIQGGSIVADPKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGIT 521
IQ +I A +YL RPWK +SRT+++ +YIGD + P G++ W G
Sbjct: 1409SIQNCTIDAAQDLANDLNSTMSYLGRPWKIYSRTVYMQSYIGDFVQPSGWLEWN---GTV 1579
Query: 522 GTDTCYYGEYNNRGPGSDVKQRVKWQGVKTITSEGAASFVPIRFFHGDDWIRVTRVPYSP 581
G DT +YGE+NN GPGS RV+W G + A +F + F G+ W+ T +PY+
Sbjct: 1580GLDTIFYGEFNNYGPGSVTNNRVQWPGHFLLNDTQAWNFTVLNFTLGNTWLPDTDIPYTE 1759
Query: 582 G 582
G
Sbjct: 1760G 1762
>TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylesterase isoform
alpha {Vigna radiata}, partial (94%)
Length = 1655
Score = 291 bits (744), Expect = 6e-79
Identities = 148/319 (46%), Positives = 203/319 (63%)
Frame = +2
Query: 248 WVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFTTITEALKHVPKKNLKPFVIYIKEGV 307
WV + RKLL + + +VVAKDG+GNF TI EA+ P + FVI+IK G
Sbjct: 41 WVSPKD--RKLLQAAVNQT-KFDLVVAKDGTGNFATIGEAIAAAPNSSATRFVIHIKAGA 211
Query: 308 YKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTASVAITGDFFVGIGIGFEN 367
Y E VEV K T+++ +GDG +T + ++N +DG TF++++ A+ GD F+ GI FEN
Sbjct: 212 YFENVEVIKKKTNLMLVGDGIGQTVVKASRNVVDGWTTFQSSTFAVVGDKFIAKGITFEN 391
Query: 368 SAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCIISGTIDFVFGDSIA 427
SAGP KHQAVA+R +D S FY+C YQDTLY H++RQFYR+C + GT+DF+FG++
Sbjct: 392 SAGPSKHQAVAVRNGADFSAFYQCSFVAYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAV 571
Query: 428 VLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVRLKNKAYLAR 487
V QNC RKP Q+ + TAQGR++ NQ TG+ I I A PV+ K+YL R
Sbjct: 572 VFQNCNLYARKPDPKQKNLFTAQGREDPNQNTGISILNCKIAAAADLIPVQSTFKSYLGR 751
Query: 488 PWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGITGTDTCYYGEYNNRGPGSDVKQRVKWQ 547
PWK +SRT++L+++I ++I P G++ W G DT +YGEY NRGPGS+ RV W
Sbjct: 752 PWKKYSRTVYLNSFIDNLIDPPGWLEWN---GTFALDTLFYGEYKNRGPGSNTSARVTWP 922
Query: 548 GVKTITSEGAASFVPIRFF 566
G K IT+ AS +R F
Sbjct: 923 GYKVITNATEASQFTVRQF 979
>TC86403 similar to PIR|B86158|B86158 hypothetical protein AAF02886.1
[imported] - Arabidopsis thaliana, partial (63%)
Length = 1929
Score = 287 bits (735), Expect = 7e-78
Identities = 174/495 (35%), Positives = 256/495 (51%), Gaps = 14/495 (2%)
Frame = +3
Query: 100 KISEGLKKTHLLQEAEKDERTKQALDTCKQVMQLSIDEFQRSLERFSNFDLNSLDRVLTS 159
K S+ + K +L + + +AL C+ + L+ D S + +N + L S
Sbjct: 249 KFSDLINK-YLQRRSTLSTTALRALQDCQSLSDLNFDFLSSSFQT-----VNKTTKFLPS 410
Query: 160 LK-----VWLSGAITYQETCLDAFENTTTD----AGKKMKEVLQTSMHMSSNGLSIINQL 210
L+ LS +T Q+TCLD ++T++ G + T ++ S +
Sbjct: 411 LQGENIQTLLSAILTNQQTCLDGLKDTSSAWSFRNGLTIPLSNDTKLYSVSLAFFTKGWV 590
Query: 211 SKTFEEMKQPAGRRLLKESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAH 270
+ + P + K +G + + E V R + L + G +
Sbjct: 591 NPKTNKTSFPNSKHSNKGFKNGRLPLKMTSKTRAIYESVSRR---KLLQSNQVGEDVVVR 761
Query: 271 --VVVAKDGSGNFTTITEALKHVPKKNLKP---FVIYIKEGVYKEYVEVTKTMTHVVFIG 325
V V++DGSGNFTTI +A+ P K++ F+IY+ GVY+EY+ + K T+++ IG
Sbjct: 762 DIVTVSQDGSGNFTTINDAIAAAPNKSVSSDGYFLIYVTAGVYEEYITIDKKKTYLMMIG 941
Query: 326 DGGRKTRITGNKNFIDGVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDR 385
DG KT ITGN + +DG TF + + A+ G FVG+ + N+AG KHQAVALR +D
Sbjct: 942 DGINKTIITGNHSVVDGWTTFGSPTFAVVGQGFVGVNMTIRNTAGAVKHQAVALRNGADL 1121
Query: 386 SIFYKCRMDGYQDTLYAHTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQC 445
S FY C +GYQDTLY H++RQFYR+C I GT+DF+FG++ V QNC R P+ Q
Sbjct: 1122STFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAKVVFQNCNLYPRLPMSGQFN 1301
Query: 446 IVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVRLKNKAYLARPWKDFSRTIFLDTYIGDM 505
+TAQGR + NQ TG I +I A YL RPWK++SRT+++ T++ ++
Sbjct: 1302AITAQGRTDPNQDTGTSIHNCTIKATDDLAASNGAVSTYLGRPWKEYSRTVYMQTFMDNV 1481
Query: 506 ITPEGYMPWQTPAGITGTDTCYYGEYNNRGPGSDVKQRVKWQGVKTITSEGAASFVPIRF 565
I G+ W G T YY E+NN GPGS RV WQG I + AA+F F
Sbjct: 1482INVAGWRAWD---GEFALSTLYYAEFNNSGPGSSTDGRVTWQGYHVINATDAANFTVANF 1652
Query: 566 FHGDDWIRVTRVPYS 580
GDDW+ T V Y+
Sbjct: 1653LLGDDWLPQTGVSYT 1697
>TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectinesterase
{Arabidopsis thaliana}, partial (34%)
Length = 691
Score = 236 bits (601), Expect = 2e-62
Identities = 122/237 (51%), Positives = 159/237 (66%)
Frame = +3
Query: 223 RRLLKESVDGEEDVLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFT 282
RRLL S EE P W+ + RKLL K + K +A +VVAKDGSG +
Sbjct: 18 RRLLSLSHQNEE-----------PNWLHHKD--RKLLQKDSDLKKKADIVVAKDGSGKYK 158
Query: 283 TITEALKHVPKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDG 342
TI+ ALKHVP K+ K VIY+K+G+Y E V V KT +V+ IGDG T ++G+ NFIDG
Sbjct: 159 TISAALKHVPNKSDKRTVIYVKKGIYYENVRVEKTKWNVMIIGDGMNVTIVSGSLNFIDG 338
Query: 343 VGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYA 402
TF TA+ A+ G F+ IGF+N+AGP+KHQAVAL +D+++FYKC MD YQDTLYA
Sbjct: 339 TPTFSTATFAVFGRNFIARDIGFKNTAGPQKHQAVALMTSADQAVFYKCSMDAYQDTLYA 518
Query: 403 HTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPT 459
H+ RQFYR+C I GT+DF+FG+S VLQNC + R+P+ QQ +TAQG+ + N T
Sbjct: 519 HSNRQFYRECNIYGTVDFIFGNSAVVLQNCNILPRQPMPGQQNTITAQGKTDPNMNT 689
>TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor (EC
3.1.1.11) (Pectin methylesterase) (PE) (P65). [Alfalfa],
partial (53%)
Length = 732
Score = 227 bits (579), Expect = 9e-60
Identities = 106/209 (50%), Positives = 143/209 (67%)
Frame = +2
Query: 354 TGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCI 413
T F + +GFENSAG KHQAVALRV +D+++FY C+M+GYQDTLY + RQFYRDC
Sbjct: 74 TSAHFTALNVGFENSAGAAKHQAVALRVTADKALFYNCQMNGYQDTLYTQSKRQFYRDCT 253
Query: 414 ISGTIDFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPK 473
I+GTIDFVFGD++ V QNC +VRKP+ NQQC+VTA GR + + + L+ Q +P+
Sbjct: 254 ITGTIDFVFGDAVGVFQNCKLIVRKPMANQQCMVTAGGRTKVDSVSALVFQNCHFTGEPE 433
Query: 474 YYPVRLKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAGITGTDTCYYGEYNN 533
++ K AYL RPW++FS+ + +D+ I + PEGYMPW G +TC Y EYNN
Sbjct: 434 VLTMQPK-IAYLGRPWRNFSKVVIVDSLIDGLFVPEGYMPWM---GNLFKETCTYLEYNN 601
Query: 534 RGPGSDVKQRVKWQGVKTITSEGAASFVP 562
+G G+ +VKW GVKTI++ AA + P
Sbjct: 602 KGAGAATNLKVKWPGVKTISAGEAAKYYP 688
>BE319906 similar to SP|Q43062|PME_ Pectinesterase PPE8B precursor (EC
3.1.1.11) (Pectin methylesterase) (PE). [Peach] {Prunus
persica}, partial (36%)
Length = 589
Score = 182 bits (463), Expect(2) = 3e-50
Identities = 81/162 (50%), Positives = 121/162 (74%)
Frame = +2
Query: 277 GSGNFTTITEALKHVPKKNLKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGN 336
GSG++ + +A+ P+ ++K +VIY+K+GVY E VE+ K +++ IG+G T I+G+
Sbjct: 2 GSGDYAKVMDAVSAAPESSMKRYVIYVKKGVYVENVEIKKKKWNIMLIGEGMDATIISGS 181
Query: 337 KNFIDGVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGY 396
+N++DG TF++A+ A++G F+ I F+N+AG EKHQAVALR SD S+FY+C + GY
Sbjct: 182 RNYVDGSTTFRSATFAVSGRGFIARDISFQNTAGAEKHQAVALRSDSDLSVFYRCGIFGY 361
Query: 397 QDTLYAHTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVRK 438
QD+LY HTMRQFYR+C ISGT+DF+FGD+ AV QNC + ++
Sbjct: 362 QDSLYTHTMRQFYRECKISGTVDFIFGDATAVFQNCQILAKE 487
Score = 34.7 bits (78), Expect(2) = 3e-50
Identities = 16/35 (45%), Positives = 21/35 (59%)
Frame = +3
Query: 437 RKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVAD 471
+K + Q+ VTAQGRK+ NQPTG + AD
Sbjct: 483 KKGMPKQKNTVTAQGRKDPNQPTGFSFHFATFTAD 587
>BI272853 weakly similar to PIR|T04359|T04 pectin methylesterase-like protein
- maize, partial (25%)
Length = 678
Score = 193 bits (491), Expect = 1e-49
Identities = 98/221 (44%), Positives = 133/221 (59%)
Frame = +1
Query: 299 FVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTASVAITGDFF 358
+ IY+K GVY EY+ + K +++ GDG KT +TG KN GV T +TA+ A T F
Sbjct: 7 YTIYVKAGVYDEYITIPKDAVNILMYGDGPGKTIVTGRKNGAAGVKTMQTATFANTALGF 186
Query: 359 VGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCIISGTI 418
+G + FEN+AGP HQAVA R Q D S C + GYQDTLY T RQFYR+C+ISGT+
Sbjct: 187 IGKAMTFENTAGPAGHQAVAFRNQGDMSALVGCHILGYQDTLYVQTNRQFYRNCVISGTV 366
Query: 419 DFVFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVR 478
DF+FG S ++Q+ T +VR P NQ +TA G TG++IQG +IV +P R
Sbjct: 367 DFIFGTSATLIQDSTIIVRMPSPNQFNTITADGSYVNKLNTGIVIQGCNIVPKAALFPQR 546
Query: 479 LKNKAYLARPWKDFSRTIFLDTYIGDMITPEGYMPWQTPAG 519
K+YL RPWK ++T+ +++ IG + W P G
Sbjct: 547 FTIKSYLGRPWKVLTKTVVMESTIG*FYSSX----WMDPLG 657
>BQ138825 similar to PIR|T49922|T49 pectin methylesterase-like protein -
Arabidopsis thaliana, partial (36%)
Length = 658
Score = 174 bits (442), Expect = 7e-44
Identities = 97/218 (44%), Positives = 131/218 (59%), Gaps = 11/218 (5%)
Frame = +1
Query: 286 EALKHVPKKNL--KPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFID-G 342
EA+ P + K FVIYIKEGVY+E V V +VVF+GDG KT ITG+ N G
Sbjct: 4 EAVNAAPDNGVDRKRFVIYIKEGVYEETVRVPLKKRNVVFLGDGIGKTVITGSANVGQPG 183
Query: 343 VGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYA 402
+ T+ +A+VA+ GD F+ + EN+AGP+ HQAVA R+ SD S+ C G QDTLYA
Sbjct: 184 MTTYNSATVAVLGDGFMAKDLTIENTAGPDAHQAVAFRLDSDLSVIENCEFLGNQDTLYA 363
Query: 403 HTMRQFYRDCIISGTIDFVFGDSIAVLQNCTFVVR----KPLENQQCIVTAQGRKEKNQP 458
H++RQFY+ C I G +DF+FG+S A+ Q+C +VR KP + + +TA GR + Q
Sbjct: 364 HSLRQFYKSCRIVGNVDFIFGNSAAIFQDCQILVRPRQLKPEKGENNAITAHGRTDPAQS 543
Query: 459 TGLIIQGGSIVADPKYYPVRLKN----KAYLARPWKDF 492
TG + Q I Y + N K YL RPWK++
Sbjct: 544 TGFVFQNCLINGTEDYMALYHSNPKVHKNYLGRPWKEY 657
>TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pectin
methylesterase {Arabidopsis thaliana}, partial (33%)
Length = 1289
Score = 136 bits (343), Expect(2) = 8e-41
Identities = 81/255 (31%), Positives = 128/255 (49%), Gaps = 10/255 (3%)
Frame = +3
Query: 254 GVRKLLNKMTGRKLQAHVVVAKDGSGNFTTITEALKHVPKKNLKPFVIYIKEGVYKEYVE 313
G+ L K K++ + V++DGS F +ITEAL + N++ +I I G Y+E +
Sbjct: 192 GLDPKLKKAESNKVR--LKVSQDGSAQFKSITEALNSIQPYNIRRVIISIAPGYYREKIV 365
Query: 314 VTKTMTHVVFIGDGGRKTRITGNKNFI----DG--VGTFKTASVAITGDFFVGIGIGFEN 367
V KT+ + F+GD ITGN DG + TF +A+VA+ +F+ I I FEN
Sbjct: 366 VPKTLPFITFLGDVRDPPTITGNDTQSVTGSDGAQLRTFNSATVAVNASYFMAININFEN 545
Query: 368 SA----GPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCIISGTIDFVFG 423
+A G + QAVA+R+ +++ FY C G QDTLY H ++ +C I G++DF+ G
Sbjct: 546 TASFPIGSKVEQAVAVRITGNKTAFYNCTFSGVQDTLYDHKGLHYFNNCTIKGSVDFICG 725
Query: 424 DSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVRLKNKA 483
++ + CT + + N +TAQ + +G + ++ D
Sbjct: 726 HGKSLYEGCTI---RSIANNMTSITAQSGSNPSYDSGFSFKNSMVIGD---------GPT 869
Query: 484 YLARPWKDFSRTIFL 498
YL RPW + FL
Sbjct: 870 YLGRPWGKLFTSCFL 914
Score = 49.3 bits (116), Expect(2) = 8e-41
Identities = 23/82 (28%), Positives = 41/82 (49%)
Frame = +1
Query: 491 DFSRTIFLDTYIGDMITPEGYMPWQTPAGITGTDTCYYGEYNNRGPGSDVKQRVKWQGVK 550
++S+ +F TY+ + + P+G+ W YYGEY GPGS+ RV W +
Sbjct: 892 NYSQVVFSYTYMDNSVLPKGWEDWNDTKRYMNA---YYGEYKCSGPGSNTAGRVPW--AR 1056
Query: 551 TITSEGAASFVPIRFFHGDDWI 572
+ + A F+ ++ G+ W+
Sbjct: 1057MLNDKEAQVFIGTQYIDGNTWL 1122
>AL368659 similar to PIR|B86158|B86 hypothetical protein AAF02886.1
[imported] - Arabidopsis thaliana, partial (25%)
Length = 495
Score = 148 bits (374), Expect = 5e-36
Identities = 72/145 (49%), Positives = 97/145 (66%), Gaps = 3/145 (2%)
Frame = +1
Query: 275 KDGSGNFTTITEALKHVPKKNLKP---FVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKT 331
+DGSGNFTTI +A+ P + F+I+I EGVY+EYV + K +++ IG+G +T
Sbjct: 61 QDGSGNFTTINDAIAAAPNNTVASDGYFLIFITEGVYEEYVSIDKKKKYLMMIGEGINQT 240
Query: 332 RITGNKNFIDGVGTFKTASVAITGDFFVGIGIGFENSAGPEKHQAVALRVQSDRSIFYKC 391
ITGN+N DG TF +A+ A+ FV + I F N+AG K+QAVALR +D S FY C
Sbjct: 241 IITGNRNVADGFTTFNSATFAVVAQGFVAVNITFRNTAGAAKNQAVALRSGADMSTFYSC 420
Query: 392 RMDGYQDTLYAHTMRQFYRDCIISG 416
+GYQDTLY H++RQFYR+C I G
Sbjct: 421 SFEGYQDTLYTHSLRQFYRECDIYG 495
>TC89457 weakly similar to SP|Q43062|PME_PRUPE Pectinesterase PPE8B
precursor (EC 3.1.1.11) (Pectin methylesterase) (PE).
[Peach] {Prunus persica}, partial (34%)
Length = 1415
Score = 108 bits (270), Expect = 6e-24
Identities = 69/235 (29%), Positives = 117/235 (49%), Gaps = 3/235 (1%)
Frame = +1
Query: 119 RTKQALDTCKQVMQLSIDEFQRSL---ERFSNFDLNSLDRVLTSLKVWLSGAITYQETCL 175
RT A+ C ++ +S+D+ +S+ ++ D NS ++ L+ WLS + Y +TC+
Sbjct: 313 RTSNAVSDCLDLLDMSLDQLNQSISAAQKPKEKD-NSTGKLNCDLRTWLSAVLVYPDTCI 489
Query: 176 DAFENTTTDAGKKMKEVLQTSMHMSSNGLSIINQLSKTFEEMKQPAGRRLLKESVDGEED 235
+ E + + S+GL + L LL E V G +D
Sbjct: 490 EGLEGSIVKG-------------LISSGLDHVMSLVAN-----------LLGEVVSGNDD 597
Query: 236 VLGHGGDFELPEWVDDRAGVRKLLNKMTGRKLQAHVVVAKDGSGNFTTITEALKHVPKKN 295
L D P W+ D N +T A VVA DGSG++ + +A+ P+ +
Sbjct: 598 QLATNKD-RFPSWIRDEDTKLLQANGVT-----ADAVVAADGSGDYAKVMDAVSAAPESS 759
Query: 296 LKPFVIYIKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTAS 350
+K +VIY+K+GVY E VE+ K +++ IG+G T I+G++N++DG TF++A+
Sbjct: 760 MKRYVIYVKKGVYVENVEIKKKKWNIMLIGEGMDATIISGSRNYVDGSTTFRSAT 924
>BI271313 similar to GP|6688852|emb pectin methyl-esterase PEF1 {Medicago
truncatula}, partial (32%)
Length = 645
Score = 93.6 bits (231), Expect(2) = 5e-23
Identities = 53/149 (35%), Positives = 83/149 (55%)
Frame = +1
Query: 5 GDAKRNKRLAIIGVSTFLLVAMVVAVTVNVGFNNKKEPGEETSKESHVSQSVKAVKTLCA 64
G + K+ A++GVS LLVAMV AV V++ G++ + + +S S K V LC
Sbjct: 106 GGQGQGKKYALLGVSCILLVAMVGAVAVSL------TKGDDKEQTAQISNSQKNVDMLCQ 267
Query: 65 PTDYKKECEDSLIAHAGNITEPKELIKIAFNITIAKISEGLKKTHLLQEAEKDERTKQAL 124
T+YK+ C SL + T+ KELIK AF+ T ++ + + + L QE KD TKQA+
Sbjct: 268 STEYKETCHKSL--ENASSTDTKELIKAAFSATAEELRKHINNSALYQELGKDSMTKQAM 441
Query: 125 DTCKQVMQLSIDEFQRSLERFSNFDLNSL 153
+ C +V+ ++D +S+ FD + L
Sbjct: 442 EICNEVLDYAVDGIHKSVGTLDQFDFHKL 528
Score = 32.7 bits (73), Expect(2) = 5e-23
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +3
Query: 169 TYQETCLDAFENTTTDAGKKM 189
++Q+TCLD F NTTT AG+ M
Sbjct: 576 SHQQTCLDGFVNTTTKAGETM 638
>TC92167 similar to PIR|T05203|T05203 pectinesterase homolog F4I10.160 -
Arabidopsis thaliana, partial (11%)
Length = 652
Score = 90.9 bits (224), Expect = 1e-18
Identities = 56/149 (37%), Positives = 83/149 (55%), Gaps = 5/149 (3%)
Frame = +1
Query: 363 IGFENSAGPEKHQAVALRVQSDRSIFYKCRMDGYQDTLYAHTMRQFYRDCIIS-GTIDFV 421
+GF N+AGPE HQAVA RVQ+DR++F CR +G+QDTLY RQF+ + DF+
Sbjct: 214 VGFRNTAGPEGHQAVAARVQADRAVFANCRFEGFQDTLYTVAHRQFFP*LHHNREQFDFI 393
Query: 422 FGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPK----YYPV 477
FGD + + L+NQ + + +++ T I Q I K ++ +
Sbjct: 394 FGDPSPLYSKTASWL---LKNQVSAKVTR*QHKEDWTTNKIQQLFYINVHLKLMTHWFLL 564
Query: 478 RLKNKAYLARPWKDFSRTIFLDTYIGDMI 506
+ K+YL R WK FSRT+ +++ IGD I
Sbjct: 565 KATVKSYLGRAWKQFSRTVVMESDIGDFI 651
Score = 73.9 bits (180), Expect = 2e-13
Identities = 55/191 (28%), Positives = 90/191 (46%), Gaps = 4/191 (2%)
Frame = +3
Query: 303 IKEGVYKEYVEVTKTMTHVVFIGDGGRKTRITGNKNFIDGVGTFKTASVAITGDFFVGIG 362
+K+ E V +T M + GDG +K+ ITG+KNF DGV TAS+ + G+ F+G+
Sbjct: 33 LKKECTDEVVTITDKMKDITIYGDGSQKSIITGSKNFRDGVTNINTASLVVLGEGFLGLA 212
Query: 363 IGFENSAGPEKHQAVALRVQSDRSIFYKCRMDG---YQDTLYAHTMRQFYRDCIISGTID 419
G + + + + + S C++ + +++ T GTI
Sbjct: 213 SGIQKHSWS*RTSSSSS*SPSRSCCV--CQLPF*RLPRHAIHSGT*TILPVAAS*QGTIR 386
Query: 420 F-VFGDSIAVLQNCTFVVRKPLENQQCIVTAQGRKEKNQPTGLIIQGGSIVADPKYYPVR 478
F ++ + QNC VV+KP Q VTAQGR + Q T +++ ++ AD PV+
Sbjct: 387 FHLW*PQPVIFQNCILVVKKPSVGQSNAVTAQGRLDNKQNTAIVLHKCTLKADDALVPVK 566
Query: 479 LKNKAYLARPW 489
+K PW
Sbjct: 567 SHSKKL---PW 590
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,229,193
Number of Sequences: 36976
Number of extensions: 209793
Number of successful extensions: 1038
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 990
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 991
length of query: 593
length of database: 9,014,727
effective HSP length: 102
effective length of query: 491
effective length of database: 5,243,175
effective search space: 2574398925
effective search space used: 2574398925
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144513.14