BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144503.1 + phase: 0 /pseudo
(718 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81046 similar to GP|17529280|gb|AAL38867.1 putative protein ki... 479 e-135
BE249202 weakly similar to GP|17998578|gb| putative Ste-20 relat... 303 1e-89
TC79505 similar to PIR|T01904|T01904 hypothetical protein T12H20... 280 1e-75
TC84173 similar to PIR|A71407|A71407 probable Ste20-like kinase ... 212 5e-55
TC88974 similar to GP|3819699|emb|CAA08758.1 BnMAP4K alpha2 {Bra... 139 8e-43
TC88790 similar to GP|22136716|gb|AAM91677.1 unknown protein {Ar... 161 9e-40
TC86478 similar to GP|3688193|emb|CAA08995.1 MAP3K alpha 1 prote... 146 2e-35
TC88861 weakly similar to GP|17027283|gb|AAL34137.1 putative pro... 130 2e-30
TC78090 GP|15528441|emb|CAC69138. MAP kinase kinase {Medicago sa... 77 2e-28
TC78699 weakly similar to PIR|G96761|G96761 probable MAP kinase ... 122 4e-28
TC77881 homologue to GP|15528439|emb|CAC69137. MEK map kinase ki... 121 8e-28
TC86297 similar to GP|15912283|gb|AAL08275.1 At1g30270/F12P21_6 ... 121 8e-28
TC76610 similar to PIR|T50802|T50802 serine/threonine protein ki... 117 1e-26
BG454756 homologue to GP|12718822|db NQK1 MAPKK {Nicotiana tabac... 116 3e-26
TC86661 similar to GP|9798599|dbj|BAB11737.1 serine/threonine pr... 116 3e-26
TC77429 similar to GP|15215664|gb|AAK91377.1 AT5g25110/T11H3_120... 115 4e-26
TC77782 similar to GP|13448037|gb|AAK26845.1 SOS2-like protein k... 114 1e-25
TC77210 similar to GP|21954717|gb|AAM83095.1 SOS2-like protein k... 110 2e-24
TC77246 similar to PIR|C71408|C71408 probable protein kinase - A... 108 5e-24
TC83196 similar to GP|7270830|emb|CAB80511.1 protein kinase like... 108 7e-24
>TC81046 similar to GP|17529280|gb|AAL38867.1 putative protein kinase
{Arabidopsis thaliana}, partial (44%)
Length = 921
Score = 479 bits (1233), Expect = e-135
Identities = 224/301 (74%), Positives = 264/301 (87%), Gaps = 1/301 (0%)
Frame = +2
Query: 63 DLNNISREAQTMVLVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEV 122
+L+ I RE QTM L+DHPN+L++HCSF + H+LWVVMPFMSGGSCLHI+K++ P+GF+E
Sbjct: 11 NLDGIRREVQTMSLIDHPNLLRAHCSFTAGHSLWVVMPFMSGGSCLHIMKSSFPEGFDEP 190
Query: 123 VIATVLREVLKGLEYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSR 182
VIATVLREVLK L YLH HGHIHRDVKAGN+L+D+ G+VK+ DFGVSAC+FD+GDRQRSR
Sbjct: 191 VIATVLREVLKALVYLHAHGHIHRDVKAGNILLDANGSVKMADFGVSACMFDTGDRQRSR 370
Query: 183 NTFVGTPCWMAPEVMEQLHGYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNA 242
NTFVGTPCWMAPEVM+QLHGY+FKADIWSFGITALELAHGHAPFSKYPP+KVLLMTLQNA
Sbjct: 371 NTFVGTPCWMAPEVMQQLHGYDFKADIWSFGITALELAHGHAPFSKYPPMKVLLMTLQNA 550
Query: 243 PPGLDYERDKKFSKSFKQMIACCLVKDPSKRPSASKLLKHSFFKQARSSDYITRTLLEGL 302
PPGLDYERDK+FSKSFK+++A CLVKDP KRPS+ KLLKH FFK AR+++Y++RT+L+GL
Sbjct: 551 PPGLDYERDKRFSKSFKELVATCLVKDPKKRPSSEKLLKHHFFKHARATEYLSRTILDGL 730
Query: 303 PALGDRMEILKRKE-DMLAQKKMPDGQMEELSQNEYKRGISGWNFNLEDMKAQASLINDF 361
LGDR LK KE D+L Q K ME+LSQ EY RGIS WNFNLED+K+QA+LI D
Sbjct: 731 APLGDRFRQLKAKEADLLMQNKALYEDMEQLSQKEYIRGISAWNFNLEDLKSQAALIQDE 910
Query: 362 D 362
D
Sbjct: 911 D 913
>BE249202 weakly similar to GP|17998578|gb| putative Ste-20 related kinase
3'-partial {Oryza sativa}, partial (5%)
Length = 666
Score = 303 bits (776), Expect(2) = 1e-89
Identities = 171/247 (69%), Positives = 171/247 (69%), Gaps = 29/247 (11%)
Frame = +3
Query: 328 QMEELSQNEYKRGISGWNFNLEDMKAQASLINDFDDAMSDISHVSSACSLTNLDAQDKQL 387
QMEELSQNEYKRGISGWNFNLEDMKAQASLINDFDDAMSDISHVSSACSLTNLDAQDKQL
Sbjct: 3 QMEELSQNEYKRGISGWNFNLEDMKAQASLINDFDDAMSDISHVSSACSLTNLDAQDKQL 182
Query: 388 PSSSHSRSQTADM-----------------------------SDDDSSITSSSHEPQTSS 418
PSSSHSRSQTADM SDDDSSITSSSHEPQTSS
Sbjct: 183 PSSSHSRSQTADMEESDEMHNQLASVPEVDSTINDVKTRIEKSDDDSSITSSSHEPQTSS 362
Query: 419 CLDDHVDHSLGDMENVGRAAEVVVATHPPLHRRGCSSSILPEVTLPPIRAESLRNDREYF 478
CLDDHVDHSLGDMENVGRAAEVVVATHPPLHRRGCSSSILPEVTLPPIRAE
Sbjct: 363 CLDDHVDHSLGDMENVGRAAEVVVATHPPLHRRGCSSSILPEVTLPPIRAE--------- 515
Query: 479 KH*CLSNFHHSHSICSDWFFFNDKYSNFNPLIFSNINDSEKLQNLSTNVSSANAILVTHT 538
SEKLQNLSTNVSSANAILVTHT
Sbjct: 516 --------------------------------------SEKLQNLSTNVSSANAILVTHT 581
Query: 539 GDDVLTE 545
GDDVLTE
Sbjct: 582 GDDVLTE 602
Score = 45.8 bits (107), Expect(2) = 1e-89
Identities = 22/24 (91%), Positives = 23/24 (95%)
Frame = +1
Query: 543 LTELPSRASKTSANSDDTDDKAKV 566
L +LPSRASKTSANSDDTDDKAKV
Sbjct: 595 LLKLPSRASKTSANSDDTDDKAKV 666
>TC79505 similar to PIR|T01904|T01904 hypothetical protein T12H20.4 -
Arabidopsis thaliana, partial (32%)
Length = 819
Score = 280 bits (716), Expect = 1e-75
Identities = 129/207 (62%), Positives = 163/207 (78%)
Frame = +2
Query: 15 KKYPIGAEHYQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCDLNNISREAQTM 74
+ Y + Y+L EE+G G SA+V+ A+ + +NE VA+K LD +R N + ++I +EAQ M
Sbjct: 197 RSYSANPQDYKLLEEVGYGASATVYHAVYLPYNEEVAVKSLDLDRCNSNFDDIRKEAQIM 376
Query: 75 VLVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKG 134
L+DH NV+KS+CSFV D LWV+MPFM+ GSCLH++K+A+PDGFEE I ++L+E LK
Sbjct: 377 SLIDHRNVVKSYCSFVVDRKLWVIMPFMAQGSCLHLMKSAYPDGFEEDAIGSILKETLKA 556
Query: 135 LEYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAP 194
L+YLH HGHIHRDVKAGN+L+DS G VKL DFGVSA +FD+GDRQRSRNTFVGTPCW
Sbjct: 557 LDYLHQHGHIHRDVKAGNILLDSNGEVKLSDFGVSASMFDTGDRQRSRNTFVGTPCWDGA 736
Query: 195 EVMEQLHGYNFKADIWSFGITALELAH 221
V++ GYNFKADIW FGITALELAH
Sbjct: 737 GVLQPGTGYNFKADIWVFGITALELAH 817
>TC84173 similar to PIR|A71407|A71407 probable Ste20-like kinase -
Arabidopsis thaliana, partial (41%)
Length = 706
Score = 212 bits (539), Expect = 5e-55
Identities = 103/218 (47%), Positives = 145/218 (66%), Gaps = 17/218 (7%)
Frame = +2
Query: 13 EKKKYPIGAEHYQLYEEIGQGVSASVHRALCVSFNEI-VAIKILDFERDNCDLNNISREA 71
E+ +YP+ + Y++ +EIG G SA V++A+C+ N VAIK +D +R DL+++ REA
Sbjct: 53 ERVQYPLDSSSYKIVDEIGAGNSAVVYKAICIPINSTPVAIKSIDLDRSRPDLDDVRREA 232
Query: 72 QTMVLVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREV 131
+T+ L+ HPN+LK+HCSF D+ LWVVMPFM+GGS I+ + +G E IA +L++
Sbjct: 233 KTLSLLSHPNILKAHCSFTVDNRLWVVMPFMAGGSLQSIISHSFQNGLTEQSIAVILKDT 412
Query: 132 LKGLEYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGD--------RQRSRN 183
L L YLH GH+HRD+K+GN+L+DS G VKL DFGVSA +++S + S N
Sbjct: 413 LNALSYLHGQGHLHRDIKSGNILVDSNGLVKLADFGVSASIYESNNSVGACSSYSSSSSN 592
Query: 184 T--------FVGTPCWMAPEVMEQLHGYNFKADIWSFG 213
+ F GTP WMAPE + +GY+FKADIWSFG
Sbjct: 593 SSSSHIFTDFAGTPYWMAPEGIHSHNGYSFKADIWSFG 706
>TC88974 similar to GP|3819699|emb|CAA08758.1 BnMAP4K alpha2 {Brassica
napus}, partial (34%)
Length = 851
Score = 139 bits (351), Expect(2) = 8e-43
Identities = 72/175 (41%), Positives = 109/175 (62%)
Frame = +3
Query: 28 EEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCDLNNISREAQTMVLVDHPNVLKSHC 87
E IGQG V++ N+ VAIK++D E D+++I +E + P + + +
Sbjct: 201 ELIGQGSFGDVYKGFDKELNKEVAIKVIDLEESEDDIDDIQKEISVLSQCRCPYITEYYG 380
Query: 88 SFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHHGHIHRD 147
SF++ LW++M +M+GGS +L++ P +E+ IA +LR++L ++YLH+ G IHRD
Sbjct: 381 SFLNQTKLWIIMEYMAGGSVADLLQSGPP--LDEMSIAYILRDLLHAVDYLHNEGKIHRD 554
Query: 148 VKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVMEQLHG 202
+KA N+L+ G VK+ DFGVSA L + R R TFVGTP WMAPEV++ L G
Sbjct: 555 IKAANILLSENGDVKVADFGVSAQLTRTISR---RKTFVGTPFWMAPEVIQNLKG 710
Score = 53.1 bits (126), Expect(2) = 8e-43
Identities = 25/54 (46%), Positives = 33/54 (60%)
Frame = +1
Query: 194 PEVMEQLHGYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLD 247
P + GYN KADIWS GITA+E+A G A + P++VL + + PP LD
Sbjct: 685 PRLFRTSKGYNEKADIWSLGITAIEMAKGGASLADLHPMRVLFIIPRENPPQLD 846
>TC88790 similar to GP|22136716|gb|AAM91677.1 unknown protein {Arabidopsis
thaliana}, partial (37%)
Length = 1595
Score = 161 bits (407), Expect = 9e-40
Identities = 80/153 (52%), Positives = 109/153 (70%), Gaps = 2/153 (1%)
Frame = +1
Query: 236 LMTLQNAPPGLDYERDKKFSKSFKQMIACCLVKDPSKRPSASKLLKHSFFKQARSSDYIT 295
LMT+QNAPPGLDY+RD+KFSKSFK+M+A CLVKD +KRPS KLLKHSFFKQA+ +
Sbjct: 28 LMTIQNAPPGLDYDRDRKFSKSFKEMVAMCLVKDQTKRPSVDKLLKHSFFKQAKPPELSV 207
Query: 296 RTLLEGLPALGDRMEILKRKE-DMLAQKKMPDGQMEELSQNEYKRGISGWNFNLEDMKAQ 354
+ L LP L R++ L+ K+ L K+MP + E +SQ+EYKRG+S WNF+++D+KAQ
Sbjct: 208 KKLFADLPPLWTRVKALQLKDAAQLTLKRMPSAEQEAISQSEYKRGVSAWNFDVDDLKAQ 387
Query: 355 ASLINDFDDAMSDISHV-SSACSLTNLDAQDKQ 386
ASL+ + DD +++I + +N D D Q
Sbjct: 388 ASLVQEEDDDIAEIKEEDENKFFNSNKDTSDSQ 486
Score = 42.7 bits (99), Expect = 5e-04
Identities = 25/62 (40%), Positives = 35/62 (56%), Gaps = 9/62 (14%)
Frame = +1
Query: 546 LPSRASKTSANS---------DDTDDKAKVPVVQQRGRFKVTSENVDPEKATPSPVLQKS 596
LP+RAS S ++ D DDK+K +VQ +GRF VTSEN+D K P+ + +
Sbjct: 856 LPNRASANSLSAPIKSSGGFRDSPDDKSKANLVQIKGRFSVTSENLDLVKDIPASSVSRR 1035
Query: 597 HS 598
S
Sbjct: 1036SS 1041
>TC86478 similar to GP|3688193|emb|CAA08995.1 MAP3K alpha 1 protein kinase
{Brassica napus}, partial (52%)
Length = 2266
Score = 146 bits (369), Expect = 2e-35
Identities = 88/274 (32%), Positives = 141/274 (51%), Gaps = 7/274 (2%)
Frame = +2
Query: 30 IGQGVSASVHRALCVSFNEIVAIKILDFERDNCD-------LNNISREAQTMVLVDHPNV 82
+G+G V+ ++ AIK + R CD L + +E + + HPN+
Sbjct: 989 LGRGTFGHVYLGFNSENGQMCAIKEV---RVGCDDQNSKECLKQLHQEIDLLNQLSHPNI 1159
Query: 83 LKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHHG 142
++ S + + +L V + ++SGGS +L+ P F+E VI R+++ GL YLH
Sbjct: 1160 VQYLGSELGEESLSVYLEYVSGGSIHKLLQEYGP--FKEPVIQNYTRQIVSGLAYLHGRN 1333
Query: 143 HIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVMEQLHG 202
+HRD+K N+L+D G +KL DFG++ + + S +F G+P WMAPEV+ +G
Sbjct: 1334 TVHRDIKGANILVDPNGEIKLADFGMAKHITSAA----SMLSFKGSPYWMAPEVVMNTNG 1501
Query: 203 YNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLDYERDKKFSKSFKQMI 262
Y+ DIWS G T +E+A P+S+Y + + + + E S K I
Sbjct: 1502 YSLPVDIWSLGCTLIEMAASKPPWSQYEGVAAIFKIGNSKDMPIIPEH---LSNDAKNFI 1672
Query: 263 ACCLVKDPSKRPSASKLLKHSFFKQARSSDYITR 296
CL +DPS RP+A KLL+H F + ++ TR
Sbjct: 1673 MLCLQRDPSARPTAQKLLEHPFIRDQSATKAATR 1774
>TC88861 weakly similar to GP|17027283|gb|AAL34137.1 putative protein kinase
{Oryza sativa (japonica cultivar-group)}, partial (34%)
Length = 1847
Score = 130 bits (327), Expect = 2e-30
Identities = 86/285 (30%), Positives = 137/285 (47%), Gaps = 10/285 (3%)
Frame = +1
Query: 23 HYQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCD----LNNISREAQTMVLVD 78
H+Q + IG+G SV+ A + A+K +D D+ + + +E + + +
Sbjct: 1021 HWQKGKLIGRGSFGSVYHATNLETGASCALKEVDLVPDDPKSTDCIKQLDQEIRILGQLH 1200
Query: 79 HPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYL 138
HPN+++ + S V L + M ++ GS ++ H E V+ R +L GL YL
Sbjct: 1201 HPNIVEYYGSEVVGDRLCIYMEYVHPGSLQKFMQD-HCGVMTESVVRNFTRHILSGLAYL 1377
Query: 139 HHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVM- 197
H IHRD+K N+L+D+ G VKL DFGVS L + + + G+P WMAPE+M
Sbjct: 1378 HSTKTIHRDIKGANLLVDASGIVKLADFGVSKILTE----KSYELSLKGSPYWMAPELMM 1545
Query: 198 -----EQLHGYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLDYERDK 252
E DIWS G T +E+ G P+S++P + + L +P + K
Sbjct: 1546 AAMKNETNPTVAMAVDIWSLGCTIIEMLTGKPPWSEFPGHQAMFKVLHRSP-----DIPK 1710
Query: 253 KFSKSFKQMIACCLVKDPSKRPSASKLLKHSFFKQARSSDYITRT 297
S + + C ++P+ RPSA+ LL H F + D I +
Sbjct: 1711 TLSPEGQDFLEQCFQRNPADRPSAAVLLTHPFVQNLHEQDVIVHS 1845
>TC78090 GP|15528441|emb|CAC69138. MAP kinase kinase {Medicago sativa subsp.
x varia}, complete
Length = 1677
Score = 77.4 bits (189), Expect(2) = 2e-28
Identities = 47/135 (34%), Positives = 75/135 (54%), Gaps = 7/135 (5%)
Frame = +3
Query: 157 SRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPE-VMEQLHGYNFKADIWSFGIT 215
++ ++L DFGVSA + + + NTF+GT +M+PE + GYN+K+DIWS G+
Sbjct: 825 TQARLRLPDFGVSAIMESTSGQA---NTFIGTYNYMSPERINGSQRGYNYKSDIWSLGLI 995
Query: 216 ALELAHGHAPFS------KYPPLKVLLMTLQNAPPGLDYERDKKFSKSFKQMIACCLVKD 269
LE A G P++ ++ + L+ T+ + PP ++FS F I+ CL KD
Sbjct: 996 LLECAMGRFPYTPPDQSERWESIFELIETIVDKPP--PSAPSEQFSSEFCSFISACLQKD 1169
Query: 270 PSKRPSASKLLKHSF 284
P R SA +L++ F
Sbjct: 1170PGSRLSAQELMELPF 1214
Score = 67.4 bits (163), Expect(2) = 2e-28
Identities = 41/135 (30%), Positives = 68/135 (50%), Gaps = 1/135 (0%)
Frame = +2
Query: 30 IGQGVSASVHRALCVSFNEIVAIKILDFERDNCDLNNISREAQTMVLVDHPNVLKSHCSF 89
+G+G V N+ A+KI+ + I++E + P V+ + SF
Sbjct: 446 VGKGNGGVVQLVQHKWTNQFFALKIIQMNIEESVRKQIAKELKINQAAQCPYVVVCYQSF 625
Query: 90 VSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHHGH-IHRDV 148
+ + +++ +M GGS +LK E ++ + ++VLKGL YLHH H IHRD+
Sbjct: 626 YDNGVISIILEYMDGGSMADLLKKVKT--IPEPYLSAICKQVLKGLIYLHHERHIIHRDL 799
Query: 149 KAGNVLIDSRGAVKL 163
K N+LI+ G VK+
Sbjct: 800 KPSNLLINHTGEVKI 844
>TC78699 weakly similar to PIR|G96761|G96761 probable MAP kinase T9L24.32
[imported] - Arabidopsis thaliana, partial (71%)
Length = 1119
Score = 122 bits (307), Expect = 4e-28
Identities = 89/276 (32%), Positives = 139/276 (50%), Gaps = 10/276 (3%)
Frame = +3
Query: 19 IGAEHYQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCDLNNISREAQTMV-LV 77
I A ++ +G G +V++ + I A+KI ++ D E +
Sbjct: 186 ISAGDFEKLSVLGHGNGGTVYKVRHKLTSIIYALKINHYDSDPTTRRRALTEVNILRRAT 365
Query: 78 DHPNVLKSHCSFVSDH-NLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLE 136
D NV+K H SF ++ ++M +M GS LK F E ++TV R++L GL
Sbjct: 366 DCTNVVKYHGSFEKPTGDVCILMEYMDSGSLETALKTTGT--FSESKLSTVARDILNGLT 539
Query: 137 YLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEV 196
YLH HRD+K N+L++ + VK+ DFGVS + G + N++VGT +M+PE
Sbjct: 540 YLHARNIAHRDIKPSNILVNIKNEVKIADFGVSKFM---GRTLEACNSYVGTCAYMSPER 710
Query: 197 ME-QLHGYN---FKADIWSFGITALELAHGHAPF---SKYPPLKVLLMTL-QNAPPGLDY 248
+ +++G N F ADIWS G+T EL G+ PF + P L+ + + PP L
Sbjct: 711 FDPEVYGGNYNGFSADIWSLGLTLFELYVGYFPFLQSGQRPDWASLMCAICFSDPPSL-- 884
Query: 249 ERDKKFSKSFKQMIACCLVKDPSKRPSASKLLKHSF 284
+ S F+ + CCL K+ +R SA++LL H F
Sbjct: 885 --PETASSEFRNFVECCLKKESGERWSAAQLLTHPF 986
>TC77881 homologue to GP|15528439|emb|CAC69137. MEK map kinase kinsae
{Medicago sativa subsp. x varia}, complete
Length = 1546
Score = 121 bits (304), Expect = 8e-28
Identities = 85/262 (32%), Positives = 134/262 (50%), Gaps = 7/262 (2%)
Frame = +2
Query: 30 IGQGVSASVHRALCVSFNEIVAIKILDFERDNCDLNNISREAQTMVLVDHPNVLKSHCSF 89
IG G +V++ + A+K++ + I RE Q + VD NV+K H +
Sbjct: 485 IGSGSGGTVYKVVHRINGRAYALKVIYGHHEESVRRQIHREIQILRDVDDVNVVKCHEMY 664
Query: 90 VSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHHGHIHRDVK 149
+ + V++ +M GGS L+ H +E +A V R++L+GL YLH +HRD+K
Sbjct: 665 DHNAEIQVLLEYMDGGS----LEGKHIP--QENQLADVARQILRGLAYLHRRHIVHRDIK 826
Query: 150 AGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPE-VMEQLHGYNFKA- 207
N+LI+SR VK+ DFGV L + D N+ VGT +M+PE + ++ + A
Sbjct: 827 PSNLLINSRKQVKIADFGVGRILNQTMD---PCNSSVGTIAYMSPERINTDINDGQYDAY 997
Query: 208 --DIWSFGITALELAHGHAPFS---KYPPLKVLLMTLQNAPPGLDYERDKKFSKSFKQMI 262
DIWS G++ LE G PF+ + ++ + PP E S F+ +
Sbjct: 998 AGDIWSLGVSILEFYMGRFPFAVGRQGDWASLMCAICMSQPP----EAPTTASPEFRDFV 1165
Query: 263 ACCLVKDPSKRPSASKLLKHSF 284
+ CL +DPS+R +AS+LL H F
Sbjct: 1166SRCLQRDPSRRWTASRLLSHPF 1231
>TC86297 similar to GP|15912283|gb|AAL08275.1 At1g30270/F12P21_6
{Arabidopsis thaliana}, partial (90%)
Length = 2043
Score = 121 bits (304), Expect = 8e-28
Identities = 88/297 (29%), Positives = 146/297 (48%), Gaps = 11/297 (3%)
Frame = +2
Query: 2 KDKDRDKEKETEKKKYPIG---------AEHYQLYEEIGQGVSASVHRALCVSFNEIVAI 52
K K+R+K+K T + + G A Y+L +G+G A V A + + VAI
Sbjct: 356 KKKEREKKKMTSRSSHISGGSSNGGRAKAGKYELGRTLGEGNFAKVKFARHIETGDHVAI 535
Query: 53 KILDFER--DNCDLNNISREAQTMVLVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHI 110
KILD E+ + + I +E TM L+ HPNV++ H + +++VM ++GG
Sbjct: 536 KILDKEKILKHKMIRQIKQEISTMKLIRHPNVIRMHEVIANRSKIFIVMELVTGGELFD- 712
Query: 111 LKAAHPDGFEEVVIATVLREVLKGLEYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSA 170
K A +E ++++ ++Y H G HRD+K N+L+D+ G +K+ DFG+SA
Sbjct: 713 -KIARSGRLKEDEARKYFQQLICAVDYCHSRGVCHRDLKPENLLLDTNGTLKVSDFGLSA 889
Query: 171 CLFDSGDRQRSRNTFVGTPCWMAPEVMEQLHGYNFKADIWSFGITALELAHGHAPFSKYP 230
L +T GTP ++APEV++ AD+WS G+ L G+ PF +
Sbjct: 890 -LPQQVREDGLLHTTCGTPNYVAPEVIQNKGYDGAIADLWSCGVILFVLMAGYLPFEEDN 1066
Query: 231 PLKVLLMTLQNAPPGLDYERDKKFSKSFKQMIACCLVKDPSKRPSASKLLKHSFFKQ 287
L+ L D+ FS S K++I L P R ++++++ +FK+
Sbjct: 1067-----LVALYKKIFKADFTCPPWFSSSAKKLIKRILDPSPITRIKIAEVIENEWFKK 1222
>TC76610 similar to PIR|T50802|T50802 serine/threonine protein kinase-like
protein - Arabidopsis thaliana, partial (73%)
Length = 1799
Score = 117 bits (294), Expect = 1e-26
Identities = 79/279 (28%), Positives = 142/279 (50%), Gaps = 5/279 (1%)
Frame = +2
Query: 13 EKKKYPIGAEHYQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCD--LNNISRE 70
+ +K I Y++ + +GQG A V+ ++ NE VAIK++ E+ + + I RE
Sbjct: 293 QHRKRNILFNKYEIGKTLGQGNFAKVYHGRNIATNENVAIKVIKKEKLKKERLMKQIKRE 472
Query: 71 AQTMVLVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLRE 130
M LV HP++++ + +++V+ ++ GG + EE + ++
Sbjct: 473 VSVMRLVRHPHIVELKEVMANKAKVFMVVEYVKGGE---LFAKVAKGKMEENIARKYFQQ 643
Query: 131 VLKGLEYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRN---TFVG 187
++ +++ H G HRD+K N+L+D +K+ DFG+SA D++RS T G
Sbjct: 644 LISAVDFCHSRGVTHRDLKPENLLLDENEDLKVSDFGLSAL----PDQRRSDGMLLTPCG 811
Query: 188 TPCWMAPEVMEQLHGYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLD 247
TP ++APEV++++ KADIWS G+ L G+ PF +++ + + D
Sbjct: 812 TPAYVAPEVLKKIGYDGSKADIWSCGVILFALLCGYLPFQGENVMRIYSKSFK-----AD 976
Query: 248 YERDKKFSKSFKQMIACCLVKDPSKRPSASKLLKHSFFK 286
Y + S K++I+ LV DP KR S ++K +F+
Sbjct: 977 YVLPEWISPGAKKLISNLLVVDPEKRFSIPDIMKDPWFQ 1093
>BG454756 homologue to GP|12718822|db NQK1 MAPKK {Nicotiana tabacum}, partial
(60%)
Length = 648
Score = 116 bits (291), Expect = 3e-26
Identities = 72/210 (34%), Positives = 116/210 (54%), Gaps = 7/210 (3%)
Frame = +2
Query: 80 PNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLH 139
P+V+ + SF ++ + +V+ +M GS + +++ + E +A V ++VL+GL YLH
Sbjct: 41 PHVVVCYHSFYNNGVISLVLEYMDRGSLVDVIRQVNT--ILEPYLAVVCKQVLQGLVYLH 214
Query: 140 HHGH-IHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVME 198
+ H IHRD+K N+L++ +G VK+ DFGVSA L R+TFVGT +M+PE +
Sbjct: 215 NERHVIHRDIKPSNLLVNHKGEVKITDFGVSAML---ASTMGQRDTFVGTYNYMSPERIS 385
Query: 199 QLHGYNFKADIWSFGITALELAHGHAPF------SKYPPLKVLLMTLQNAPPGLDYERDK 252
Y++ DIWS G+ LE A G P+ +P LL + +PP
Sbjct: 386 G-STYDYSCDIWSLGMVVLECAIGRFPYIQSEDQQAWPSFYELLQAIVESPP--PSAPPD 556
Query: 253 KFSKSFKQMIACCLVKDPSKRPSASKLLKH 282
+FS F ++ C+ KDP +R ++ +LL H
Sbjct: 557 QFSPEFCSFVSSCIKKDPRERSTSLELLDH 646
>TC86661 similar to GP|9798599|dbj|BAB11737.1 serine/threonine protein
kinase {Arabidopsis thaliana}, partial (58%)
Length = 1840
Score = 116 bits (291), Expect = 3e-26
Identities = 72/266 (27%), Positives = 137/266 (51%), Gaps = 2/266 (0%)
Frame = +2
Query: 24 YQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCD--LNNISREAQTMVLVDHPN 81
Y+L + +G G A V+ A + VA+K+++ ++ + ++ RE M + HPN
Sbjct: 305 YELGKLLGCGAFAKVYHARNTENGQSVAVKVINKKKISATGLAGHVKREISIMSKLRHPN 484
Query: 82 VLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHH 141
+++ H + ++ VM F GG K A+ F E + + ++++ + Y H H
Sbjct: 485 IVRLHEVLATKTKIYFVMEFAKGGELF--AKIANKGRFSEDLSRRLFQQLISAVGYCHSH 658
Query: 142 GHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVMEQLH 201
G HRD+K N+L+D +G +K+ DFG+SA + + +T GTP ++APE++ +
Sbjct: 659 GVFHRDLKPENLLLDDKGNLKVSDFGLSA-VKEQVRIDGMLHTLCGTPAYVAPEILAKRG 835
Query: 202 GYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLDYERDKKFSKSFKQM 261
K DIWS GI L G+ PF+ P L V+ + + +++ + FS K+
Sbjct: 836 YDGAKVDIWSCGIILFVLVAGYLPFND-PNLMVMYRKIYSG----EFKCPRWFSPDLKRF 1000
Query: 262 IACCLVKDPSKRPSASKLLKHSFFKQ 287
++ + +P R + ++L+ +F++
Sbjct: 1001LSRLMDTNPETRITVDEILRDPWFRK 1078
>TC77429 similar to GP|15215664|gb|AAK91377.1 AT5g25110/T11H3_120
{Arabidopsis thaliana}, partial (79%)
Length = 1869
Score = 115 bits (289), Expect = 4e-26
Identities = 78/266 (29%), Positives = 134/266 (50%), Gaps = 2/266 (0%)
Frame = +3
Query: 24 YQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCD--LNNISREAQTMVLVDHPN 81
Y++ +G+G A V+ A +S E VAIK++D ++ + + I RE M LV HPN
Sbjct: 270 YEMGRVLGKGTFAKVYYAKEISTGEGVAIKVVDKDKVKKEGMMEQIKREISVMRLVKHPN 449
Query: 82 VLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHH 141
++ + + VM + GG + + F+E + ++++ ++Y H
Sbjct: 450 IVNLKEVMATKTKILFVMEYARGGE---LFEKVAKGKFKEELARKYFQQLISAVDYCHSR 620
Query: 142 GHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVMEQLH 201
G HRD+K N+L+D +K+ DFG+SA L + + +T GTP ++APEV+ +
Sbjct: 621 GVSHRDLKPENLLLDENENLKVSDFGLSA-LPEHLRQDGLLHTQCGTPAYVAPEVVRKRG 797
Query: 202 GYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLDYERDKKFSKSFKQM 261
FKAD WS G+ L G PF L+++ N +Y+ FS K++
Sbjct: 798 YSGFKADTWSCGVILYALLAGFLPFQHEN-----LISMYNKVFKEEYQFPPWFSPESKRL 962
Query: 262 IACCLVKDPSKRPSASKLLKHSFFKQ 287
I+ LV DP +R + S ++ +F++
Sbjct: 963 ISKILVADPERRITISSIMNVPWFQK 1040
>TC77782 similar to GP|13448037|gb|AAK26845.1 SOS2-like protein kinase PKS6
{Arabidopsis thaliana}, partial (77%)
Length = 1236
Score = 114 bits (286), Expect = 1e-25
Identities = 77/266 (28%), Positives = 131/266 (48%), Gaps = 2/266 (0%)
Frame = +2
Query: 24 YQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFER--DNCDLNNISREAQTMVLVDHPN 81
Y++ + IG+G A V A V + VAIKILD + ++ + RE M +++HPN
Sbjct: 191 YEMGKTIGEGSFAKVKLAKNVENGDYVAIKILDRNHVLKHNMMDQLKREISAMKIINHPN 370
Query: 82 VLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHHH 141
V+K S +++V+ ++GG K A +E + ++++ ++Y H
Sbjct: 371 VIKIFEVMASKTKIYIVLELVNGGELFD--KIATHGRLKEDEARSYFQQLINAVDYCHSR 544
Query: 142 GHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVMEQLH 201
G HRD+K N+L+D+ G +K+ DFG+S S T GTP ++APEV+
Sbjct: 545 GVYHRDLKPENLLLDTNGVLKVSDFGLSTY---SQQEDELLRTACGTPNYVAPEVLNDRG 715
Query: 202 GYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPGLDYERDKKFSKSFKQM 261
+DIWS G+ L G+ PF + P L L + A D++ FS K++
Sbjct: 716 YVGSSSDIWSCGVILFVLMAGYLPFDE-PNLIALYRKIGRA----DFKCPSWFSPEAKKL 880
Query: 262 IACCLVKDPSKRPSASKLLKHSFFKQ 287
+ L +P R ++L+ +F++
Sbjct: 881 LTSILNPNPLTRIKIPEILEDEWFRK 958
>TC77210 similar to GP|21954717|gb|AAM83095.1 SOS2-like protein kinase
{Glycine max}, partial (88%)
Length = 1831
Score = 110 bits (275), Expect = 2e-24
Identities = 79/282 (28%), Positives = 135/282 (47%), Gaps = 3/282 (1%)
Frame = +1
Query: 9 EKETEKKKYPIGAEHYQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFER--DNCDLNN 66
EKE + Y+L +G G A V+ A ++ + VA+K++ E+ +
Sbjct: 310 EKENPNSHSALLDGKYELGRVLGHGTFAKVYHAKNLNTGKNVAMKVVGKEKVIKVGMIEQ 489
Query: 67 ISREAQTMVLVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIAT 126
I RE M +V HPN+++ H S +++ M + GG + + +E V
Sbjct: 490 IKREISVMKMVKHPNIVQLHEVMASKSKIYIAMELVRGGELFNKIVKGR---LKEDVARV 660
Query: 127 VLREVLKGLEYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRS-RNTF 185
++++ +++ H G HRD+K N+L+D G +K+ DFG+ C F RQ +T
Sbjct: 661 YFQQLISAVDFCHSRGVYHRDLKPENLLLDEDGNLKVSDFGL--CTFSEHLRQDGLLHTT 834
Query: 186 VGTPCWMAPEVMEQLHGYNFKADIWSFGITALELAHGHAPFSKYPPLKVLLMTLQNAPPG 245
GTP +++PEV+ + KADIWS G+ L G PF L+ +
Sbjct: 835 CGTPAYVSPEVIAKKGYDGAKADIWSCGVILYVLLAGFLPFQDDN-----LVAMYKKIYR 999
Query: 246 LDYERDKKFSKSFKQMIACCLVKDPSKRPSASKLLKHSFFKQ 287
D++ FS +++I L +P+ R S SK++ S+FK+
Sbjct: 1000GDFKCPPWFSLEARRLITKLLDPNPNTRISISKIMDSSWFKK 1125
>TC77246 similar to PIR|C71408|C71408 probable protein kinase - Arabidopsis
thaliana, partial (65%)
Length = 1715
Score = 108 bits (271), Expect = 5e-24
Identities = 79/270 (29%), Positives = 132/270 (48%), Gaps = 5/270 (1%)
Frame = +3
Query: 24 YQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFER--DNCDLNNISREAQTMV-LVDHP 80
YQL + +G+G A V++A + VA+K++D + D I RE M L +HP
Sbjct: 87 YQLTKFLGRGNFAKVYQARSLIDGSTVAVKVIDKSKTVDASMEPRIVREIDAMRRLQNHP 266
Query: 81 NVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGLEYLHH 140
N+LK H + +++++ F GG LK A F E + ++++ L + H
Sbjct: 267 NILKIHEVLATKTKIYLIVDFAGGGELF--LKLARRRKFPEALARRYFQQLVSALCFCHR 440
Query: 141 HGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPEVMEQL 200
+G HRD+K N+L+D+ G +K+ DFG+SA + +T GTP + APE++ ++
Sbjct: 441 NGIAHRDLKPQNLLLDAEGNLKVSDFGLSA--LPEQLKNGLLHTACGTPAYTAPEILGRI 614
Query: 201 HGYNFKADIWSFGITALELAHGHAPF--SKYPPLKVLLMTLQNAPPGLDYERDKKFSKSF 258
KAD WS G+ L GH PF S P + + DY+ + SK
Sbjct: 615 GYDGSKADAWSCGVILYVLLAGHLPFDDSNIPAMCKRITR-------RDYQFPEWISKPA 773
Query: 259 KQMIACCLVKDPSKRPSASKLLKHSFFKQA 288
+ +I L +P R + +++FK++
Sbjct: 774 RYLIYQLLDPNPKTRIRLENVFGNNWFKKS 863
>TC83196 similar to GP|7270830|emb|CAB80511.1 protein kinase like protein
{Arabidopsis thaliana}, partial (66%)
Length = 1484
Score = 108 bits (270), Expect = 7e-24
Identities = 74/218 (33%), Positives = 109/218 (49%), Gaps = 1/218 (0%)
Frame = +3
Query: 17 YPIGAEHYQLYEEIGQGVSASVHRALCVSFNEIVAIKILDFERDNCDLNN-ISREAQTMV 75
+ I +H + +I +++ + S + VAIK+L E + ++ ++E M
Sbjct: 852 WEIDPKHLKYGTQIASASYGELYKGIYCS--QEVAIKVLKAEHVSSEMQKEFAQEVYIMR 1025
Query: 76 LVDHPNVLKSHCSFVSDHNLWVVMPFMSGGSCLHILKAAHPDGFEEVVIATVLREVLKGL 135
V H NV++ + L +V FMSGGS L F+ + V +V KG+
Sbjct: 1026 KVRHKNVVQFMGACTQPPRLCIVTEFMSGGSVYDYLHK-QKGFFKFPTVLKVAIDVSKGM 1202
Query: 136 EYLHHHGHIHRDVKAGNVLIDSRGAVKLGDFGVSACLFDSGDRQRSRNTFVGTPCWMAPE 195
YLH H IHRD+KA N+L+D G VK+ DFGV+ SG GT WMAPE
Sbjct: 1203 NYLHQHNIIHRDLKAANLLMDENGVVKVADFGVARVRAQSG----VMTAETGTYRWMAPE 1370
Query: 196 VMEQLHGYNFKADIWSFGITALELAHGHAPFSKYPPLK 233
V+E Y+ KAD++SFG+ EL G P+ PL+
Sbjct: 1371 VIEH-KPYDHKADVFSFGVVLWELLTGKLPYEFLTPLQ 1481
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.135 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,267,646
Number of Sequences: 36976
Number of extensions: 364894
Number of successful extensions: 3122
Number of sequences better than 10.0: 512
Number of HSP's better than 10.0 without gapping: 2648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2769
length of query: 718
length of database: 9,014,727
effective HSP length: 103
effective length of query: 615
effective length of database: 5,206,199
effective search space: 3201812385
effective search space used: 3201812385
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC144503.1