BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144484.9 + phase: 0
(177 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76995 similar to GP|15215604|gb|AAK91347.1 AT3g52300/T25B15_70... 360 e-100
TC76994 similar to GP|15215604|gb|AAK91347.1 AT3g52300/T25B15_70... 271 1e-91
AJ499989 similar to GP|15215604|gb| AT3g52300/T25B15_70 {Arabido... 107 2e-24
AJ500899 similar to GP|15215604|gb| AT3g52300/T25B15_70 {Arabido... 104 2e-23
TC85290 similar to GP|7767653|gb|AAF69150.1| F27F5.2 {Arabidopsi... 32 0.13
TC91772 weakly similar to GP|17380998|gb|AAL36311.1 unknown prot... 30 0.38
TC91697 similar to GP|10177356|dbj|BAB10699. contains similarity... 30 0.50
TC86549 similar to GP|7331143|gb|AAF60293.1| chaperonin 21 precu... 29 1.1
TC76713 similar to PIR|S33622|S33622 ADR6 protein - soybean, par... 28 2.5
TC85314 weakly similar to PIR|T45969|T45969 mRNA capping enzyme-... 27 4.2
TC88705 similar to GP|10177834|dbj|BAB11263. gene_id:MCD7.9~unkn... 27 5.5
TC89459 weakly similar to GP|9280317|dbj|BAB01696.1 oxylase-like... 27 5.5
TC83950 26 7.2
CB891165 homologue to PIR|H82068|H820 RNA polymerase-associated ... 26 7.2
AJ499968 weakly similar to GP|23495961|gb| hypothetical protein ... 26 9.4
BQ158031 similar to GP|10441918|gb| unknown {Homo sapiens}, part... 26 9.4
>TC76995 similar to GP|15215604|gb|AAK91347.1 AT3g52300/T25B15_70
{Arabidopsis thaliana}, complete
Length = 913
Score = 360 bits (925), Expect = e-100
Identities = 177/177 (100%), Positives = 177/177 (100%)
Frame = +1
Query: 1 MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPE 60
MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPE
Sbjct: 79 MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPE 258
Query: 61 PIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKE 120
PIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKE
Sbjct: 259 PIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKE 438
Query: 121 SERLEKEIVNVQSLKKRISTMTADEYFAEHPELKKKFDDEIRNDNWGYSHYKEEQNA 177
SERLEKEIVNVQSLKKRISTMTADEYFAEHPELKKKFDDEIRNDNWGYSHYKEEQNA
Sbjct: 439 SERLEKEIVNVQSLKKRISTMTADEYFAEHPELKKKFDDEIRNDNWGYSHYKEEQNA 609
>TC76994 similar to GP|15215604|gb|AAK91347.1 AT3g52300/T25B15_70
{Arabidopsis thaliana}, partial (93%)
Length = 661
Score = 271 bits (693), Expect(2) = 1e-91
Identities = 135/135 (100%), Positives = 135/135 (100%)
Frame = +2
Query: 1 MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPE 60
MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPE
Sbjct: 41 MSGTVKKVTDVAFKAGKKIDWDGMAKLLVTDEARREFFNLRRAFDEVNTQLETKFSQEPE 220
Query: 61 PIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKE 120
PIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKE
Sbjct: 221 PIDWEYYRKGIGTRLVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKE 400
Query: 121 SERLEKEIVNVQSLK 135
SERLEKEIVNVQSLK
Sbjct: 401 SERLEKEIVNVQSLK 445
Score = 81.6 bits (200), Expect(2) = 1e-91
Identities = 35/38 (92%), Positives = 36/38 (94%)
Frame = +1
Query: 140 TMTADEYFAEHPELKKKFDDEIRNDNWGYSHYKEEQNA 177
T+ DEYFAEHPELKKKFDDEIRNDNWGYSHYKEEQNA
Sbjct: 433 TIAEDEYFAEHPELKKKFDDEIRNDNWGYSHYKEEQNA 546
>AJ499989 similar to GP|15215604|gb| AT3g52300/T25B15_70 {Arabidopsis
thaliana}, partial (29%)
Length = 505
Score = 107 bits (267), Expect = 2e-24
Identities = 53/53 (100%), Positives = 53/53 (100%)
Frame = +3
Query: 79 YKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNV 131
YKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNV
Sbjct: 3 YKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNV 161
>AJ500899 similar to GP|15215604|gb| AT3g52300/T25B15_70 {Arabidopsis
thaliana}, partial (27%)
Length = 502
Score = 104 bits (260), Expect = 2e-23
Identities = 52/52 (100%), Positives = 52/52 (100%)
Frame = +3
Query: 80 KQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNV 131
KQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNV
Sbjct: 3 KQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNV 158
>TC85290 similar to GP|7767653|gb|AAF69150.1| F27F5.2 {Arabidopsis
thaliana}, partial (32%)
Length = 1558
Score = 32.0 bits (71), Expect = 0.13
Identities = 19/67 (28%), Positives = 32/67 (47%)
Frame = +3
Query: 109 ELKEAEEKSLKESERLEKEIVNVQSLKKRISTMTADEYFAEHPELKKKFDDEIRNDNWGY 168
E K EEK+ KE ER EKE + K++ ++ H + + D++ D GY
Sbjct: 741 ERKREEEKAKKEKEREEKEKRKEKEKKEKEREREKEKSKERHKKDESDSDNQDMTDGHGY 920
Query: 169 SHYKEEQ 175
K+++
Sbjct: 921 REEKKKE 941
>TC91772 weakly similar to GP|17380998|gb|AAL36311.1 unknown protein
{Arabidopsis thaliana}, partial (17%)
Length = 1237
Score = 30.4 bits (67), Expect = 0.38
Identities = 22/66 (33%), Positives = 33/66 (49%), Gaps = 2/66 (3%)
Frame = +2
Query: 93 DTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNVQSLKKRISTMTADEYFAEHPE 152
D + KP+ +K + EK L +S +++ V V SLK T T DE+ E P+
Sbjct: 632 DVGSSSLKPEDKKKKTHVKGSSEKGLAKSSAEDEDKVTVSSLKSATKT-TKDEHSEETPK 808
Query: 153 --LKKK 156
LK+K
Sbjct: 809 TTLKRK 826
>TC91697 similar to GP|10177356|dbj|BAB10699. contains similarity to unknown
protein~gb|AAF20227.1~gene_id:K15N18.15 {Arabidopsis
thaliana}, partial (76%)
Length = 862
Score = 30.0 bits (66), Expect = 0.50
Identities = 16/30 (53%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = +2
Query: 108 VELKEAEEKSLKESERLEKEIVNV-QSLKK 136
VELKE EEK+ KE ++L+KE+ + + LKK
Sbjct: 275 VELKEKEEKASKEIKQLKKELSTLTEGLKK 364
>TC86549 similar to GP|7331143|gb|AAF60293.1| chaperonin 21 precursor
{Lycopersicon esculentum}, partial (92%)
Length = 1134
Score = 28.9 bits (63), Expect = 1.1
Identities = 16/33 (48%), Positives = 21/33 (63%), Gaps = 3/33 (9%)
Frame = +1
Query: 88 IPKFVDTVTPQY---KPKFDALLVELKEAEEKS 117
I K V P+Y KP D +LV++KEAEEK+
Sbjct: 220 IVKAATVVAPKYTAIKPLGDRVLVKIKEAEEKT 318
>TC76713 similar to PIR|S33622|S33622 ADR6 protein - soybean, partial (67%)
Length = 1790
Score = 27.7 bits (60), Expect = 2.5
Identities = 20/53 (37%), Positives = 29/53 (53%), Gaps = 1/53 (1%)
Frame = -1
Query: 80 KQHYESIEIPKFVDTVTPQYKPKFDALLVE-LKEAEEKSLKESERLEKEIVNV 131
K H+E I +P V TP P+F L V+ LK+ + ESE L K++ N+
Sbjct: 926 KAHFERINLPGIVAFQTPWGDPQFHHLHVQVLKDLA*Q--WESECLAKQLPNI 774
>TC85314 weakly similar to PIR|T45969|T45969 mRNA capping enzyme-like protein
- Arabidopsis thaliana, partial (16%)
Length = 1179
Score = 26.9 bits (58), Expect = 4.2
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -2
Query: 61 PIDWEYYRKGIGTRLVDMYKQHY 83
PID + YRK I + D +QHY
Sbjct: 1091 PIDLDNYRKWIQNEICDATRQHY 1023
>TC88705 similar to GP|10177834|dbj|BAB11263. gene_id:MCD7.9~unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 1685
Score = 26.6 bits (57), Expect = 5.5
Identities = 19/93 (20%), Positives = 44/93 (46%)
Frame = +1
Query: 75 LVDMYKQHYESIEIPKFVDTVTPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNVQSL 134
+V K H E I+ + + + + + K + KEAEEK+ ++ + +E +++
Sbjct: 10 VVKQLKDHKEQIDKAEEKERLQKEKEEK------QKKEAEEKANEKQVKTNEEDTGIENE 171
Query: 135 KKRISTMTADEYFAEHPELKKKFDDEIRNDNWG 167
++ S + + + H +++ K D + D G
Sbjct: 172 AEKHSDIEDNFAASIHEKIEVKEDSPVDQDEAG 270
>TC89459 weakly similar to GP|9280317|dbj|BAB01696.1 oxylase-like protein
{Arabidopsis thaliana}, partial (70%)
Length = 1302
Score = 26.6 bits (57), Expect = 5.5
Identities = 14/52 (26%), Positives = 28/52 (52%)
Frame = +3
Query: 96 TPQYKPKFDALLVELKEAEEKSLKESERLEKEIVNVQSLKKRISTMTADEYF 147
+PQY P+F A+L E+ ++E E+L ++ + +L + +E+F
Sbjct: 507 SPQYPPQFRAIL-------EEYIQEVEKLAYRLLELIALSLGLEAKRFEEFF 641
>TC83950
Length = 688
Score = 26.2 bits (56), Expect = 7.2
Identities = 25/81 (30%), Positives = 36/81 (43%), Gaps = 6/81 (7%)
Frame = +3
Query: 103 FDALLVELKEAEEKSLKESERLEKEIVNVQSLKKRISTMTAD----EYFAEHP--ELKKK 156
F +LL +++ S S L + +IST+ A+ F EHP EL K
Sbjct: 249 FSSLLDSFRQSSFSSKGRSVSLNDTV--------KISTLPAEGDESSRFEEHPFLELHKN 404
Query: 157 FDDEIRNDNWGYSHYKEEQNA 177
D E+ NW SH+K +A
Sbjct: 405 GDFEVDVLNW--SHFKVANSA 461
>CB891165 homologue to PIR|H82068|H820 RNA polymerase-associated protein HepA
VC2506 [imported] - Vibrio cholerae, partial (1%)
Length = 692
Score = 26.2 bits (56), Expect = 7.2
Identities = 14/57 (24%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = -2
Query: 109 ELKEAEEKSLKESERLEKEIVNVQSLKKRIST--MTADEYFAEHPELKKKFDDEIRN 163
+ ++ ++ ++E E++E++I + K I + E H + KKK +DE +N
Sbjct: 406 QFEDEAKRLVEEGEKVEEKIRILVEQKNSIELEKIKLKESMERHEDEKKKLEDEAKN 236
>AJ499968 weakly similar to GP|23495961|gb| hypothetical protein {Plasmodium
falciparum 3D7}, partial (19%)
Length = 342
Score = 25.8 bits (55), Expect = 9.4
Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 3/40 (7%)
Frame = -3
Query: 109 ELKEAEEKSLKESER---LEKEIVNVQSLKKRISTMTADE 145
E KEAEEK+LKE E L++ +++ + ++ S T +E
Sbjct: 127 ERKEAEEKALKEQEEKALLKEGVIDKKEEEEPKSKATPEE 8
>BQ158031 similar to GP|10441918|gb| unknown {Homo sapiens}, partial (3%)
Length = 725
Score = 25.8 bits (55), Expect = 9.4
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = +3
Query: 73 TRLVDMYKQHYESIEIPKFVDTVTPQYKP 101
TR V +YK ++ I I K + V P YKP
Sbjct: 3 TRYVPIYKPIHKPIPIYKPIPKVIPIYKP 89
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.132 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,228,899
Number of Sequences: 36976
Number of extensions: 43240
Number of successful extensions: 213
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 213
length of query: 177
length of database: 9,014,727
effective HSP length: 90
effective length of query: 87
effective length of database: 5,686,887
effective search space: 494759169
effective search space used: 494759169
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144484.9