BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144481.8 + phase: 0 /pseudo
(119 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF631937 similar to GP|10177621|db phytoene dehydrogenase-like {... 33 0.016
TC84181 similar to SP|Q9SJA7|SOX_ARATH Potential sarcosine oxida... 33 0.021
CB892167 similar to GP|18086398|gb AT3g23510/MEE5_5 {Arabidopsis... 32 0.035
TC86349 homologue to SP|P31023|DLDH_PEA Dihydrolipoamide dehydro... 32 0.046
TC85684 homologue to GP|3175990|emb|CAA06731.1 GDP dissociation ... 31 0.078
AW585176 similar to GP|14532474|gb| At1g62600/T3P18_16 {Arabidop... 30 0.10
TC90079 similar to GP|5454204|gb|AAD43619.1| T3P18.18 {Arabidops... 30 0.13
BG648549 similar to PIR|E85359|E853 hypothetical protein AT4g307... 30 0.13
TC77092 squalene monooxygenase 2 [Medicago truncatula] 30 0.13
BF643468 weakly similar to PIR|F96682|F96 hypothetical protein F... 29 0.23
BE322390 similar to GP|17978954|gb At1g12570/T12C24_9 {Arabidops... 28 0.51
TC91882 similar to GP|10176714|dbj|BAB09944. dimethylaniline mon... 28 0.51
TC88260 homologue to GP|15215696|gb|AAK91394.1 AT3g16950/K14A17_... 28 0.66
BG454521 similar to SP|P23618|THI4 Thiazole biosynthetic enzyme ... 28 0.66
BQ750853 similar to GP|13562018|gb fibroin 3 {Plectreurys tristi... 28 0.66
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 28 0.66
TC80236 similar to GP|8953395|emb|CAB96668.1 putative protein {A... 27 1.1
TC86973 similar to GP|15912233|gb|AAL08250.1 At2g41680/T32G6.20 ... 27 1.1
TC76978 homologue to GP|14487954|gb|AAK63815.1 early light induc... 26 1.9
TC83364 similar to GP|13786442|gb|AAK39567.1 putative electron t... 26 1.9
>BF631937 similar to GP|10177621|db phytoene dehydrogenase-like {Arabidopsis
thaliana}, partial (26%)
Length = 688
Score = 33.1 bits (74), Expect = 0.016
Identities = 17/32 (53%), Positives = 20/32 (62%)
Frame = +3
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
K D +I+G G G AAA LA GL V VLE+
Sbjct: 108 KWDALIIGGGHNGLTAAAYLARGGLSVAVLER 203
>TC84181 similar to SP|Q9SJA7|SOX_ARATH Potential sarcosine oxidase (EC
1.5.3.1). [Mouse-ear cress] {Arabidopsis thaliana},
partial (20%)
Length = 761
Score = 32.7 bits (73), Expect = 0.021
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +3
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
DV+IVG+G G A A GLK LVLE+ ++ R S
Sbjct: 57 DVIIVGAGVMGSSTAYYAAKRGLKTLVLEQFDFLHHRGSS 176
>CB892167 similar to GP|18086398|gb AT3g23510/MEE5_5 {Arabidopsis thaliana},
partial (28%)
Length = 817
Score = 32.0 bits (71), Expect = 0.035
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +1
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
V +VG G G V+A VLA G+ V++ EK +Y
Sbjct: 4 VAVVGGGISGLVSAYVLAKGGVNVVLYEKEDY 99
>TC86349 homologue to SP|P31023|DLDH_PEA Dihydrolipoamide dehydrogenase
mitochondrial precursor (EC 1.8.1.4), complete
Length = 1785
Score = 31.6 bits (70), Expect = 0.046
Identities = 16/30 (53%), Positives = 18/30 (59%)
Frame = +3
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
DVVI+G G GG VAA A G K +EK
Sbjct: 168 DVVIIGGGPGGYVAAIKAAQLGFKTTCIEK 257
>TC85684 homologue to GP|3175990|emb|CAA06731.1 GDP dissociation inhibitor
{Cicer arietinum}, complete
Length = 1849
Score = 30.8 bits (68), Expect = 0.078
Identities = 13/49 (26%), Positives = 29/49 (58%)
Frame = +2
Query: 59 EIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYF 107
+ D + + + DV+++G+G V + +L+ GLKVL +++ +Y+
Sbjct: 107 DCDFRSKKKKMDEEYDVIVLGTGLKECVLSGLLSVDGLKVLHMDRNDYY 253
>AW585176 similar to GP|14532474|gb| At1g62600/T3P18_16 {Arabidopsis
thaliana}, partial (21%)
Length = 476
Score = 30.4 bits (67), Expect = 0.10
Identities = 19/63 (30%), Positives = 34/63 (53%)
Frame = +3
Query: 42 MNEDDLSLAQSLSNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVL 101
MNE +++ +K ++ + + +L + V +G+G GG VAA L G +V+V
Sbjct: 63 MNEHVHTVSIQFDSKPMKFIMSTATPLLTPR-HVFFIGAGAGGLVAARELRREGHQVVVF 239
Query: 102 EKG 104
E+G
Sbjct: 240 ERG 248
>TC90079 similar to GP|5454204|gb|AAD43619.1| T3P18.18 {Arabidopsis
thaliana}, partial (38%)
Length = 677
Score = 30.0 bits (66), Expect = 0.13
Identities = 14/31 (45%), Positives = 18/31 (57%)
Frame = +3
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
V ++G+G G VAA L G V+V EK N
Sbjct: 21 VAVIGAGVSGMVAAKELQQEGHNVIVFEKNN 113
>BG648549 similar to PIR|E85359|E853 hypothetical protein AT4g30720
[imported] - Arabidopsis thaliana, partial (7%)
Length = 736
Score = 30.0 bits (66), Expect = 0.13
Identities = 16/33 (48%), Positives = 20/33 (60%)
Frame = +2
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKG 104
K + IVGSG G AA VLA G V ++E+G
Sbjct: 629 KPKIAIVGSGPSGLFAALVLAELGADVTLIERG 727
>TC77092 squalene monooxygenase 2 [Medicago truncatula]
Length = 1910
Score = 30.0 bits (66), Expect = 0.13
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +3
Query: 63 DANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
+ S+ L DV+IVG+G G A L G +V ++E+
Sbjct: 273 EIKSDKLNGDADVIIVGAGIAGAALAHTLGKDGRRVHIIER 395
>BF643468 weakly similar to PIR|F96682|F96 hypothetical protein F12P19.2
[imported] - Arabidopsis thaliana, partial (33%)
Length = 655
Score = 29.3 bits (64), Expect = 0.23
Identities = 14/31 (45%), Positives = 18/31 (57%)
Frame = +2
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
V ++G+G G VAA L G V+V EK N
Sbjct: 89 VAVIGAGVSGLVAAKELQQEGHNVVVFEKNN 181
>BE322390 similar to GP|17978954|gb At1g12570/T12C24_9 {Arabidopsis
thaliana}, partial (20%)
Length = 599
Score = 28.1 bits (61), Expect = 0.51
Identities = 19/42 (45%), Positives = 23/42 (54%)
Frame = +3
Query: 63 DANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKG 104
DA S + D +IVG G G AA L S +VLVLE+G
Sbjct: 171 DATSAPIISYYDYIIVGGGTAGCPLAATL-SQNHRVLVLERG 293
>TC91882 similar to GP|10176714|dbj|BAB09944. dimethylaniline
monooxygenase-like protein {Arabidopsis thaliana},
partial (47%)
Length = 800
Score = 28.1 bits (61), Expect = 0.51
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
+V ++G+G G VAA + G KV+VLE+ +
Sbjct: 59 NVCVIGAGPAGLVAAREIRKEGHKVIVLEQNH 154
>TC88260 homologue to GP|15215696|gb|AAK91394.1 AT3g16950/K14A17_7
{Arabidopsis thaliana}, partial (80%)
Length = 2274
Score = 27.7 bits (60), Expect = 0.66
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = +3
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLE 102
D++I+G+G GG AA GLK ++E
Sbjct: 306 DLLIIGAGVGGHGAALHAVEKGLKTAIVE 392
>BG454521 similar to SP|P23618|THI4 Thiazole biosynthetic enzyme
mitochondrial precursor (Stress- inducible protein
sti35)., partial (42%)
Length = 636
Score = 27.7 bits (60), Expect = 0.66
Identities = 14/34 (41%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +3
Query: 72 KCDVVIVGSGCGGGVAAAVLAS-SGLKVLVLEKG 104
+CDV+IVG+G G A L+ +KV ++E+G
Sbjct: 315 ECDVIIVGAGSAGLSCAYELSKHPDVKVAIIEQG 416
>BQ750853 similar to GP|13562018|gb fibroin 3 {Plectreurys tristis}, partial
(0%)
Length = 692
Score = 27.7 bits (60), Expect = 0.66
Identities = 21/71 (29%), Positives = 33/71 (45%), Gaps = 2/71 (2%)
Frame = +3
Query: 32 RPLEKGIVEAMNEDDLSLAQSLSNKGLEIDI--DANSNILKVKCDVVIVGSGCGGGVAAA 89
RP + ED+LS ++ K +D +A + V + V+ S GGG A
Sbjct: 381 RPKPRAAAPLEPEDELSSLPTMPVKTAPLDCVAEAVEVAVAVPAEEVVFWSVGGGGTARV 560
Query: 90 VLASSGLKVLV 100
VLA+ G ++V
Sbjct: 561 VLAAVGRTLVV 593
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 27.7 bits (60), Expect = 0.66
Identities = 15/32 (46%), Positives = 19/32 (58%)
Frame = +2
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
DVVIVG+G G A L G++ LVLE +
Sbjct: 122 DVVIVGAGIAGLATALGLHRLGVRSLVLESSD 217
>TC80236 similar to GP|8953395|emb|CAB96668.1 putative protein {Arabidopsis
thaliana}, partial (89%)
Length = 1378
Score = 26.9 bits (58), Expect = 1.1
Identities = 16/32 (50%), Positives = 18/32 (56%)
Frame = +1
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEK 103
K VIVG GG +A L +G VLVLEK
Sbjct: 10 KPKAVIVGGSIGGISSAHALILAGWDVLVLEK 105
>TC86973 similar to GP|15912233|gb|AAL08250.1 At2g41680/T32G6.20
{Arabidopsis thaliana}, partial (38%)
Length = 923
Score = 26.9 bits (58), Expect = 1.1
Identities = 14/29 (48%), Positives = 18/29 (61%)
Frame = +1
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLE 102
+VVI+GSG G AA A + LK +V E
Sbjct: 241 NVVIIGSGPAGYTAAIYAARANLKPVVFE 327
>TC76978 homologue to GP|14487954|gb|AAK63815.1 early light inducible
protein {Medicago sativa}, complete
Length = 953
Score = 26.2 bits (56), Expect = 1.9
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = -3
Query: 65 NSNILKVKCDVVIVGSGCGGGVAA--AVLASSG 95
+ N + + + V VG GCGGGV A V+ S+G
Sbjct: 396 SENFVLIFGERV*VGCGCGGGVGADVGVIGSTG 298
>TC83364 similar to GP|13786442|gb|AAK39567.1 putative electron transfer
oxidoreductase {Oryza sativa}, partial (11%)
Length = 381
Score = 26.2 bits (56), Expect = 1.9
Identities = 18/41 (43%), Positives = 23/41 (55%), Gaps = 6/41 (14%)
Frame = +2
Query: 71 VKCDVVIVGSGCGGGVAAAVLA------SSGLKVLVLEKGN 105
++ DVVIVG+G G AA L + L V VLEKG+
Sbjct: 188 IEYDVVIVGAGPAGLSAAIRLKQLCRENDTDLSVCVLEKGS 310
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,139,457
Number of Sequences: 36976
Number of extensions: 34796
Number of successful extensions: 153
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of query: 119
length of database: 9,014,727
effective HSP length: 95
effective length of query: 24
effective length of database: 5,502,007
effective search space: 132048168
effective search space used: 132048168
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC144481.8