BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.8 + phase: 0 /pseudo
(879 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 185 7e-47
BQ149324 153 3e-37
BG647708 weakly similar to GP|13786450|gb| putative reverse tran... 105 1e-22
TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative ret... 87 1e-21
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 73 5e-13
BE248682 similar to GP|18568269|gb putative gag-pol polyprotein ... 69 6e-12
BG584442 62 4e-11
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 55 2e-07
BQ148771 40 0.003
TC83341 weakly similar to GP|8978076|dbj|BAA98104.1 gene_id:K14A... 33 0.62
TC89193 weakly similar to GP|21593109|gb|AAM65058.1 MtN3-like pr... 32 0.81
BF647389 32 1.1
AW980456 31 2.4
BM813581 similar to PIR|C86393|C863 hypothetical protein AAF9858... 30 3.1
TC77272 weakly similar to GP|19110917|gb|AAL85347.1 EDS1-like pr... 30 4.0
BQ144224 similar to PIR|T33925|T339 hypothetical protein Y66H1A.... 29 6.9
BG586254 29 9.0
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 185 bits (469), Expect = 7e-47
Identities = 91/219 (41%), Positives = 133/219 (60%)
Frame = -3
Query: 59 AFVSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRF 118
AFV GR+I DN + +I+HY++ K A+K D++K YD I W++LR V+T++ F
Sbjct: 664 AFVPGRAISDNVLITHKILHYLRQSGAKKHVSMAVKTDMTKAYDRIAWNFLREVLTRLGF 485
Query: 119 SDQWVKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKD 178
W+ WIM CV TV+Y L+NG +++P RGLRQGDPLSPYLFI+C E LS L ++
Sbjct: 484 HGIWISWIMECVSTVSYSFLINGGPQGRVLPSRGLRQGDPLSPYLFILCTEVLSGLCQQA 305
Query: 179 EARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQK 238
+G + GVK+ N P I+HLLFADD F ++N + + +I+ Y A G+ +N K
Sbjct: 304 LRKGTLPGVKVARNCPPINHLLFADDTMFFGKSNASSCAILLSIMDKYRAASGRCIN*TK 125
Query: 239 SEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLGLPSMI 277
S I S + + + L + GTGKYLG +++
Sbjct: 124 SAITFSSKTSQAIIDRVKGELKIAKEGGTGKYLGYRNIL 8
>BQ149324
Length = 374
Score = 153 bits (386), Expect = 3e-37
Identities = 81/121 (66%), Positives = 89/121 (72%), Gaps = 1/121 (0%)
Frame = +2
Query: 583 TNSCTSKR*RSYMPYELYTVHDEQ*GCTSFIFFNVQVA*MFGACFLSFLLFLFCYNKIWI 642
TNS TSKR*+S+M Y LYTV+ Q*G T FFN QVA*MFG CF SFLLFLFCYNKIWI
Sbjct: 5 TNSRTSKR*KSHMSYGLYTVYGGQ*GYTFI*FFNAQVA*MFGVCFHSFLLFLFCYNKIWI 184
Query: 643 TRILSSKHYMIYLRTMHLYFVVCCGVYGNKETTKCGMR*LMLQ-FMLSREHKLWSMIDKM 701
RIL SKHYMIYL M LYFVVCCGVY NKETTKCG + Q +++ R S + KM
Sbjct: 185 VRILFSKHYMIYLMKMQLYFVVCCGVYRNKETTKCGNEVIDTQAYVVERAKTYAS*LAKM 364
Query: 702 H 702
H
Sbjct: 365 H 367
>BG647708 weakly similar to GP|13786450|gb| putative reverse transcriptase
{Oryza sativa}, partial (9%)
Length = 708
Score = 105 bits (261), Expect = 1e-22
Identities = 52/120 (43%), Positives = 79/120 (65%)
Frame = +1
Query: 149 PGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLF 208
P +GLRQGDPLSPYLFI+C LS L++++ + ++G+++ + P I+HLLFADD LF
Sbjct: 4 PEKGLRQGDPLSPYLFILCANVLSGLLKREGNKQNLHGIQVARSDPKITHLLFADDSLLF 183
Query: 209 FRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCSRNVLTTVHNNIASILGVQVVIGTG 268
R N+ +A+ + +L +Y+ A GQ VN +KSE+ S+N V N ++ Q+ I TG
Sbjct: 184 ARANLTEAATIMQVLHSYQSASGQLVNFEKSEVSYSQN----VPNQEKEMICQQIAIKTG 351
>TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative retroelement
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 625
Score = 86.7 bits (213), Expect = 4e-17
Identities = 47/97 (48%), Positives = 61/97 (62%)
Frame = +3
Query: 199 LLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCSRNVLTTVHNNIASI 258
LLF R A M+ IL YE G+A++L+KS I+CSRNV + +I I
Sbjct: 279 LLFEGAPLRSLRVEEHHAQIMKNILILYEEDSGKAISLRKS*IYCSRNVPDILKTSITYI 458
Query: 259 LGVQVVIGTGKYLGLPSMIGRSKKSTFSFIKDRIWKK 295
LGVQ ++GT KYLGLPSMIGR + +TFS IK +W+K
Sbjct: 459 LGVQFMLGTCKYLGLPSMIGRDRTTTFSSIKGGVWQK 569
Score = 84.7 bits (208), Expect(2) = 1e-21
Identities = 40/70 (57%), Positives = 51/70 (72%)
Frame = +2
Query: 128 MCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGV 187
MCVE+ +Y+VLVN D + IIP RGL+QGD LSPY+FIICVEGLS L+ + RG+ +G
Sbjct: 101 MCVESNDYYVLVNNDAVDPIIPSRGLQQGDHLSPYIFIICVEGLSFLIPHAKERGDTHGT 280
Query: 188 KICNNAPIIS 197
I AP +S
Sbjct: 281 SI*RGAPPVS 310
Score = 37.4 bits (85), Expect(2) = 1e-21
Identities = 14/32 (43%), Positives = 23/32 (71%)
Frame = +1
Query: 95 LDISKTYDMIDWSYLRGVMTKMRFSDQWVKWI 126
L ISK Y+ +D YL+ +M KM F+++W+ W+
Sbjct: 1 LYISKVYNRVD*DYLKEIMIKMGFNNRWIYWM 96
Score = 30.0 bits (66), Expect = 4.0
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +1
Query: 281 KKSTFSFIKDRIWKKINLWSSKSLSKAGREVLI 313
++ F ++ KKIN WS LSKA REV+I
Sbjct: 526 EQQLFLLLRVECGKKINSWSDICLSKACREVMI 624
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 72.8 bits (177), Expect = 5e-13
Identities = 64/208 (30%), Positives = 91/208 (42%)
Frame = +2
Query: 116 MRFSDQWVKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALV 175
M F W KWI CV T VLVNG + ++ + L Q + Y F
Sbjct: 380 MSFLVLWRKWIKECVSTATTSVLVNGSPTNVLM--KSLVQTQLFTRYSF----------- 520
Query: 176 RKDEARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVN 235
G +N V ++SHL FA+D L N +RA L + G VN
Sbjct: 521 ------GVVNPV-------VVSHLQFANDTLLLETKNWANIRALRAALVIF*AMSGLKVN 661
Query: 236 LQKSEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLGLPSMIGRSKKSTFSFIKDRIWKK 295
KS + C N+ + + AS+L +V YLG+P + S + I +RI +
Sbjct: 662 FHKSGLVCV-NIAPSWLSEAASVLSWKVGKVPFLYLGMPIEGNSRRLSFWEPIVNRIKAR 838
Query: 296 INLWSSKSLSKAGREVLIKFVLQSIPTY 323
+ W+S+ LS GR VL+K VL S+ Y
Sbjct: 839 LTGWNSRFLSFGGRLVLLKSVLTSLSVY 922
>BE248682 similar to GP|18568269|gb putative gag-pol polyprotein {Zea mays},
partial (1%)
Length = 441
Score = 69.3 bits (168), Expect = 6e-12
Identities = 34/91 (37%), Positives = 50/91 (54%)
Frame = +3
Query: 151 RGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFFR 210
RGL+QGDPL+P+LF++ EG+S L++ R G + +SHL +ADD
Sbjct: 24 RGLKQGDPLAPFLFLLVAEGISGLMKNAVNRNLFQGFDVKRGGTRVSHLQYADDTLCIGM 203
Query: 211 TNVDQASKMRAILSTYERAYGQAVNLQKSEI 241
VD ++A+L +E A G VN KS +
Sbjct: 204 PTVDNLWTLKALLQGFEMASGLKVNFHKSSL 296
>BG584442
Length = 775
Score = 61.6 bits (148), Expect(2) = 4e-11
Identities = 33/64 (51%), Positives = 42/64 (65%)
Frame = +1
Query: 294 KKINLWSSKSLSKAGREVLIKFVLQSIPTYFMSLFTHPVSLCDEIERIMNSFWWGHSGGQ 353
KKIN +K LSK EV+IK+ LQSI +Y MS+F S DEIE+IMN+F W H G
Sbjct: 379 KKINF*RNKCLSKVM*EVMIKYALQSISSYVMSIFLLLNSQVDEIEKIMNTFSWVHVGEN 558
Query: 354 SRGL 357
+G+
Sbjct: 559 RKGM 570
Score = 25.0 bits (53), Expect(2) = 4e-11
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = +3
Query: 276 MIGRSKKSTFSFIKDRI 292
M+ S K+TFS+IKD I
Sbjct: 324 MLSTSNKATFSYIKDEI 374
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 54.7 bits (130), Expect = 2e-07
Identities = 27/85 (31%), Positives = 47/85 (54%)
Frame = +3
Query: 59 AFVSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRF 118
AFV GR I DN + + E++ K G +K+D+ K YD +W +++ +M ++ F
Sbjct: 357 AFVKGRVIFDNIILSHELVKSYSRK--GISPRCMVKIDLXKAYDSXEWPFIKHLMLELGF 530
Query: 119 SDQWVKWIMMCVETVNYFVLVNGDV 143
++V W+M + T +Y NGD+
Sbjct: 531 PYKFVNWVMAXLTTASYTFNXNGDL 605
>BQ148771
Length = 680
Score = 40.4 bits (93), Expect = 0.003
Identities = 22/69 (31%), Positives = 37/69 (52%)
Frame = -3
Query: 280 SKKSTFSFIKDRIWKKINLWSSKSLSKAGREVLIKFVLQSIPTYFMSLFTHPVSLCDEIE 339
+KKS FS + ++ + W + LS A R L K V++++P Y M P + +EI+
Sbjct: 618 TKKSRFS-VYYQVHVMLANWKANHLSLARRVTLAKSVIEAVPLYPMMTTIIPKACIEEIQ 442
Query: 340 RIMNSFWWG 348
++ F WG
Sbjct: 441 KLQRKFVWG 415
>TC83341 weakly similar to GP|8978076|dbj|BAA98104.1 gene_id:K14A3.4~unknown
protein {Arabidopsis thaliana}, partial (80%)
Length = 1253
Score = 32.7 bits (73), Expect = 0.62
Identities = 18/40 (45%), Positives = 25/40 (62%)
Frame = -3
Query: 611 SFIFFNVQVA*MFGACFLSFLLFLFCYNKIWITRILSSKH 650
S +FFN+ V +F FL LFLF + K +IT L+S+H
Sbjct: 660 SLVFFNIIV--IFFFLFLFLFLFLFLFLKSFITNPLASRH 547
>TC89193 weakly similar to GP|21593109|gb|AAM65058.1 MtN3-like protein
{Arabidopsis thaliana}, partial (54%)
Length = 1017
Score = 32.3 bits (72), Expect = 0.81
Identities = 15/38 (39%), Positives = 19/38 (49%)
Frame = -3
Query: 626 CFLSFLLFLFCYNKIWITRILSSKHYMIYLRTMHLYFV 663
CF FLLF + I IL HY + L +H+ FV
Sbjct: 892 CFFLFLLFFILHCSFDINNILKRDHYYLQLILIHIIFV 779
>BF647389
Length = 404
Score = 32.0 bits (71), Expect = 1.1
Identities = 30/90 (33%), Positives = 41/90 (45%), Gaps = 1/90 (1%)
Frame = +2
Query: 269 KYLGLPSMIGRSKKSTFSFIKDRIWKKINLWSSKSLSKAGREVLIKFVLQSIPTYFMSLF 328
+YLG+P + K +K +I +I SSK LS AG LIK VL + Y+ +F
Sbjct: 41 RYLGVP--LSSKKLYVIQRVK-KIICRIEN*SSKLLSYAGSLQLIKIVLFGVQPYWSQVF 211
Query: 329 THPVSLCDEIERIMNSFW-WGHSGGQSRGL 357
P + I+ F G SG R L
Sbjct: 212 VLP*KVIKLIQTTCRIFL*TGKSGTSKRAL 301
>AW980456
Length = 779
Score = 30.8 bits (68), Expect = 2.4
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = -1
Query: 529 KS*RVLCS*CIPFLCTRIT*YIPFQGTRLLESNLEAQDSSKGKKIYVANL*KLLT 583
K+ +LC C+ FLC + +R L NLEA +S G K+ + ++* L T
Sbjct: 185 KAWEILCKECLSFLCRGTLRFFLPSPSR*LVRNLEA*STS*GSKLSLEDV*GLFT 21
>BM813581 similar to PIR|C86393|C863 hypothetical protein AAF98586.1
[imported] - Arabidopsis thaliana, partial (10%)
Length = 672
Score = 30.4 bits (67), Expect = 3.1
Identities = 19/56 (33%), Positives = 34/56 (59%), Gaps = 7/56 (12%)
Frame = -2
Query: 206 FLFFRTNVDQASKMRAI--LSTYERAYGQA-----VNLQKSEIFCSRNVLTTVHNN 254
F FFRT + + S++R I ++ ++R + ++ K+ +F + NVLTTVHN+
Sbjct: 347 FYFFRT-ISEVSRIRIIRNITNFDRFHLPVFVQLLLSKLKTIVFSANNVLTTVHNS 183
>TC77272 weakly similar to GP|19110917|gb|AAL85347.1 EDS1-like protein
{Nicotiana benthamiana}, partial (51%)
Length = 2168
Score = 30.0 bits (66), Expect = 4.0
Identities = 13/29 (44%), Positives = 18/29 (61%), Gaps = 2/29 (6%)
Frame = -1
Query: 636 CYNKIWITRILSSKHYMIYLR--TMHLYF 662
C N W TR +S KHY++ L +HL+F
Sbjct: 536 CQNSHWTTRRVSCKHYLLLLLHCPLHLFF 450
>BQ144224 similar to PIR|T33925|T339 hypothetical protein Y66H1A.4 -
Caenorhabditis elegans, partial (9%)
Length = 822
Score = 29.3 bits (64), Expect = 6.9
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -2
Query: 344 SFWWGHSGGQSRGLTGFLGINCLCTRMMEV*VSKI 378
S +G G RG+TG GI C+CT V +S +
Sbjct: 725 SLMYGSGVGALRGITGLRGIVCVCTTRRYVRMSGV 621
>BG586254
Length = 499
Score = 28.9 bits (63), Expect = 9.0
Identities = 15/42 (35%), Positives = 20/42 (46%), Gaps = 3/42 (7%)
Frame = -3
Query: 634 LFCYNKIWITRILSSKHYMIY---LRTMHLYFVVCCGVYGNK 672
L +KIW LS+ Y+ Y + T H+Y C G G K
Sbjct: 434 LLTESKIWFLNHLSNVKYLTYN*IINTRHIY*NFCLGYLGEK 309
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.354 0.159 0.546
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,950,004
Number of Sequences: 36976
Number of extensions: 434722
Number of successful extensions: 5604
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1729
Number of HSP's successfully gapped in prelim test: 312
Number of HSP's that attempted gapping in prelim test: 3793
Number of HSP's gapped (non-prelim): 2226
length of query: 879
length of database: 9,014,727
effective HSP length: 105
effective length of query: 774
effective length of database: 5,132,247
effective search space: 3972359178
effective search space used: 3972359178
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.5 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144477.8