BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.14 + phase: 1 /pseudo
(190 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA990302 weakly similar to GP|10177640|db contains similarity to... 173 4e-44
TC80263 similar to GP|20466540|gb|AAM20587.1 putative protein {A... 149 5e-37
TC81945 weakly similar to GP|10177640|dbj|BAB10787. contains sim... 137 2e-33
TC80262 similar to GP|10176717|dbj|BAB09947. contains similarity... 107 3e-24
AW692409 similar to GP|10176717|db contains similarity to hepara... 94 4e-20
TC82933 similar to GP|20466540|gb|AAM20587.1 putative protein {A... 41 2e-04
CB894342 similar to GP|20197988|gb expressed protein {Arabidopsi... 34 0.030
TC90068 similar to GP|20197988|gb|AAD22321.2 expressed protein {... 33 0.087
BI310284 homologue to GP|2707297|gb|A special lobe-specific silk... 32 0.19
TC88196 similar to GP|18650639|gb|AAL75889.1 AT4g27020/F10M23_36... 29 1.3
BQ137719 28 2.2
TC87762 similar to GP|21537142|gb|AAM61483.1 unknown {Arabidopsi... 27 4.8
TC78065 similar to PIR|T47856|T47856 hypothetical protein T8B10.... 27 4.8
TC86934 similar to PIR|T04630|T04630 synaptobrevin homolog F10N7... 27 6.3
>CA990302 weakly similar to GP|10177640|db contains similarity to
heparanase~gene_id:MGG23.2 {Arabidopsis thaliana},
partial (42%)
Length = 786
Score = 173 bits (438), Expect = 4e-44
Identities = 82/100 (82%), Positives = 89/100 (89%)
Frame = +2
Query: 86 DVVTHHIYNLGPGVDDHITEKILDPTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAW 145
+VVTHHIYNLGPG D HI E+ILDP+ L+GVAG FSSLKNVLQ S T+A+AWVGEAGGA
Sbjct: 2 NVVTHHIYNLGPGGDPHIVERILDPSSLNGVAGIFSSLKNVLQSSRTSAQAWVGEAGGAS 181
Query: 146 NSGHHLVSDAFVNSFWYFDQLGMSATYSTKTYCRQTLIGG 185
SGHHLVSDAFVNSFWY DQLGMSATY T+TYCRQTLIGG
Sbjct: 182 GSGHHLVSDAFVNSFWYLDQLGMSATYGTRTYCRQTLIGG 301
>TC80263 similar to GP|20466540|gb|AAM20587.1 putative protein {Arabidopsis
thaliana}, partial (46%)
Length = 1041
Score = 149 bits (377), Expect = 5e-37
Identities = 73/147 (49%), Positives = 93/147 (62%), Gaps = 1/147 (0%)
Frame = +3
Query: 1 AKSFIRYTVSKNYTIHGWELGNELCGKGIGISISPYQYANDATILRNIVQEVYREVVQKP 60
A FI+YTVSK Y I WELGNEL GKGIG S+ QY D L+ I+ +Y + KP
Sbjct: 600 AYDFIKYTVSKGYKIDAWELGNELSGKGIGASVGVTQYGKDLIKLKRILDVLYEKSRFKP 779
Query: 61 LIIAPGGFFDANWFKKFLNRS-EKLADVVTHHIYNLGPGVDDHITEKILDPTYLDGVAGT 119
++APGGF++ W+ K L S + +V+THH+YNLGPG D H+ KILDP L V
Sbjct: 780 SLVAPGGFYEKEWYDKLLQVSGSGIINVLTHHLYNLGPGSDGHLEGKILDPNRLSKVETI 959
Query: 120 FSSLKNVLQRSSTTAKAWVGEAGGAWN 146
FS+L +Q+ AWVGEAGGA+N
Sbjct: 960 FSNLSETIQKHGPWTSAWVGEAGGAYN 1040
>TC81945 weakly similar to GP|10177640|dbj|BAB10787. contains similarity to
heparanase~gene_id:MGG23.2 {Arabidopsis thaliana},
partial (38%)
Length = 1031
Score = 137 bits (346), Expect = 2e-33
Identities = 62/90 (68%), Positives = 75/90 (82%)
Frame = +1
Query: 1 AKSFIRYTVSKNYTIHGWELGNELCGKGIGISISPYQYANDATILRNIVQEVYREVVQKP 60
A+SFIRYTV+K YTIHGWE GNELCG GIG S++ QYA+D +LR I+Q+VYR V KP
Sbjct: 760 AESFIRYTVAKKYTIHGWEFGNELCGGGIGTSVAADQYASDVIVLRKIIQDVYRGVKPKP 939
Query: 61 LIIAPGGFFDANWFKKFLNRSEKLADVVTH 90
LIIAPGGFFD NWFK+FLN+S++ +VVTH
Sbjct: 940 LIIAPGGFFDENWFKEFLNKSDESTNVVTH 1029
>TC80262 similar to GP|10176717|dbj|BAB09947. contains similarity to
heparanase~gene_id:MXM12.7 {Arabidopsis thaliana},
partial (34%)
Length = 1195
Score = 107 bits (267), Expect = 3e-24
Identities = 49/86 (56%), Positives = 60/86 (68%)
Frame = +3
Query: 100 DDHITEKILDPTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDAFVNS 159
D H+ KI DP L V FS+L +Q+ AWVGEAGGA+NSG VS+ F+NS
Sbjct: 186 DGHLEGKIFDPNRLSTVETIFSNLSETIQKHGPWTSAWVGEAGGAYNSGGRCVSNTFLNS 365
Query: 160 FWYFDQLGMSATYSTKTYCRQTLIGG 185
FWY DQLG+++TY+TK YCRQTLIGG
Sbjct: 366 FWYLDQLGIASTYNTKVYCRQTLIGG 443
>AW692409 similar to GP|10176717|db contains similarity to
heparanase~gene_id:MXM12.7 {Arabidopsis thaliana},
partial (24%)
Length = 662
Score = 93.6 bits (231), Expect = 4e-20
Identities = 44/76 (57%), Positives = 53/76 (68%)
Frame = +1
Query: 110 PTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDAFVNSFWYFDQLGMS 169
P + V TF +L +Q+ A AWVGEAGGA NSG +S+ FVNSFWY DQLGM+
Sbjct: 1 PNRMIKVETTFKNLSETIQKHGPWASAWVGEAGGAANSGGIGISNTFVNSFWYLDQLGMA 180
Query: 170 ATYSTKTYCRQTLIGG 185
+ Y+TK YCRQTLIGG
Sbjct: 181 SNYNTKVYCRQTLIGG 228
>TC82933 similar to GP|20466540|gb|AAM20587.1 putative protein {Arabidopsis
thaliana}, partial (26%)
Length = 770
Score = 41.2 bits (95), Expect = 2e-04
Identities = 18/28 (64%), Positives = 19/28 (67%)
Frame = +2
Query: 1 AKSFIRYTVSKNYTIHGWELGNELCGKG 28
A FI+YTVSK Y I WE GNEL G G
Sbjct: 626 AYDFIKYTVSKGYKIDAWEFGNELSGTG 709
>CB894342 similar to GP|20197988|gb expressed protein {Arabidopsis thaliana},
partial (64%)
Length = 838
Score = 34.3 bits (77), Expect = 0.030
Identities = 29/101 (28%), Positives = 42/101 (40%), Gaps = 7/101 (6%)
Frame = +3
Query: 91 HIYNLGPGVDDHITEKILDPTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWNSGHH 150
H+ L PGV+ + K+ + G T + L R+ W S HH
Sbjct: 492 HLGQLSPGVEYSLHRKLGVAVFCLGALQTLALLFRPNARNKFRKY---------WKSYHH 644
Query: 151 LVSDA-----FVNSFWYFDQLGMSATYSTKTYC--RQTLIG 184
V + FVN F F+ +G S +Y+ +YC TLIG
Sbjct: 645 FVGYSCVVLGFVNVFQGFEVMGASRSYAKLSYCLGLSTLIG 767
>TC90068 similar to GP|20197988|gb|AAD22321.2 expressed protein {Arabidopsis
thaliana}, partial (88%)
Length = 1352
Score = 32.7 bits (73), Expect = 0.087
Identities = 30/95 (31%), Positives = 43/95 (44%), Gaps = 7/95 (7%)
Frame = +1
Query: 97 PGVDDHITEKILDPTYLDGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDA- 155
PGV+ + K+ + G T + +L R +TT K W S HH V +
Sbjct: 829 PGVEYGLHRKLGIAVFCLGALQTLA----LLFRPNTTNKF-----RKYWKSYHHFVGYSC 981
Query: 156 ----FVNSFWYFDQLGMSATYSTKTYC--RQTLIG 184
FVN F F+ +G S +Y+ +YC TLIG
Sbjct: 982 VVLGFVNVFQGFEVIGASRSYAKLSYCLGLSTLIG 1086
>BI310284 homologue to GP|2707297|gb|A special lobe-specific silk protein
ssp160 {Chironomus thummi}, partial (1%)
Length = 694
Score = 31.6 bits (70), Expect = 0.19
Identities = 15/35 (42%), Positives = 19/35 (53%), Gaps = 1/35 (2%)
Frame = +3
Query: 59 KPLIIAPGGFFDANWFKKFLN-RSEKLADVVTHHI 92
KP IAPGG + WF K + + DV+TH I
Sbjct: 528 KPSPIAPGGLYQKEWFDKLIRVLGSGVNDVLTHRI 632
>TC88196 similar to GP|18650639|gb|AAL75889.1 AT4g27020/F10M23_360
{Arabidopsis thaliana}, partial (96%)
Length = 1810
Score = 28.9 bits (63), Expect = 1.3
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = +2
Query: 114 DGVAGTFSSLKNVLQRSSTTAKAWVGEAGGAWNSGHHLVSDAFVNSFWYFD 164
+GV+G ++ V R S K WVGE+G G +++ + +W F+
Sbjct: 812 NGVSGAYAGHTAVCLRDSD-GKLWVGESGHENEKGEDIIAMIAWDEWWDFE 961
>BQ137719
Length = 469
Score = 28.1 bits (61), Expect = 2.2
Identities = 12/42 (28%), Positives = 21/42 (49%)
Frame = +2
Query: 34 SPYQYANDATILRNIVQEVYREVVQKPLIIAPGGFFDANWFK 75
SP Y N A+++R + + V +K ++ P G A W +
Sbjct: 206 SPSSYINHASLIRKLCERVKVYPKKKEEMVKPSGAITAKWIE 331
>TC87762 similar to GP|21537142|gb|AAM61483.1 unknown {Arabidopsis
thaliana}, partial (65%)
Length = 1594
Score = 26.9 bits (58), Expect = 4.8
Identities = 9/27 (33%), Positives = 16/27 (58%)
Frame = +3
Query: 137 WVGEAGGAWNSGHHLVSDAFVNSFWYF 163
W + +W+SG H++ F +FW+F
Sbjct: 918 WFRDTNSSWDSGKHVLL-CFSTTFWHF 995
>TC78065 similar to PIR|T47856|T47856 hypothetical protein T8B10.110 -
Arabidopsis thaliana, partial (71%)
Length = 1286
Score = 26.9 bits (58), Expect = 4.8
Identities = 20/63 (31%), Positives = 28/63 (43%), Gaps = 3/63 (4%)
Frame = +1
Query: 65 PGGFFDANW---FKKFLNRSEKLADVVTHHIYNLGPGVDDHITEKILDPTYLDGVAGTFS 121
P G D N +K+ E L+D + + D H TE +L T+ +GV FS
Sbjct: 505 PAGTVDKNVARVYKELPKWEEPLSDTRARYEKIVKDLADKHPTENLLLVTHGEGVGVAFS 684
Query: 122 SLK 124
S K
Sbjct: 685 SFK 693
>TC86934 similar to PIR|T04630|T04630 synaptobrevin homolog F10N7.40 -
Arabidopsis thaliana, complete
Length = 1208
Score = 26.6 bits (57), Expect = 6.3
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +1
Query: 122 SLKNVLQRSSTTAKAWVGEAGGAWNSG 148
SL + + ST + AGGAWNSG
Sbjct: 73 SLSSEPESKSTIQNGYTLRAGGAWNSG 153
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.137 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,172,495
Number of Sequences: 36976
Number of extensions: 76456
Number of successful extensions: 350
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 349
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 349
length of query: 190
length of database: 9,014,727
effective HSP length: 91
effective length of query: 99
effective length of database: 5,649,911
effective search space: 559341189
effective search space used: 559341189
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144477.14