
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.10 - phase: 0 /pseudo
(200 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82122 weakly similar to GP|15010776|gb|AAK74047.1 At2g11890/F2... 188 1e-48
AL376110 weakly similar to GP|15010776|gb| At2g11890/F23M2.5 {Ar... 162 8e-41
TC78583 similar to GP|4097585|gb|AAD09518.1| NTGP4 {Nicotiana ta... 28 1.8
BE323735 similar to GP|21592923|gb induced upon wounding stress ... 27 5.3
TC85524 similar to SP|P93508|CRTC_RICCO Calreticulin precursor. ... 27 5.3
BG645999 similar to GP|16930745|gb NADH-ubiquinone oxidoreductas... 27 5.3
TC77464 similar to GP|13272433|gb|AAK17155.1 unknown protein {Ar... 27 6.9
TC87770 similar to GP|14970762|emb|CAC44460. protein tyrosine ph... 26 9.0
>TC82122 weakly similar to GP|15010776|gb|AAK74047.1 At2g11890/F23M2.5
{Arabidopsis thaliana}, partial (26%)
Length = 943
Score = 188 bits (477), Expect = 1e-48
Identities = 94/101 (93%), Positives = 94/101 (93%)
Frame = +1
Query: 1 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 60
MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE
Sbjct: 640 MEVEVKLRLPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAILRLRFYGDDE 819
Query: 61 RCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVDEPGKL 101
RCVVSLKAKAVLVDGVS VE EDLDP IG CVDEPGKL
Sbjct: 820 RCVVSLKAKAVLVDGVSXVEXXXEDLDPXIGXXCVDEPGKL 942
>AL376110 weakly similar to GP|15010776|gb| At2g11890/F23M2.5 {Arabidopsis
thaliana}, partial (37%)
Length = 404
Score = 162 bits (410), Expect = 8e-41
Identities = 78/78 (100%), Positives = 78/78 (100%)
Frame = +2
Query: 123 GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGI 182
GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGI
Sbjct: 2 GFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECESSDPEEAKRILEEFLKENGI 181
Query: 183 DYSYSVASKFAIFRAGKL 200
DYSYSVASKFAIFRAGKL
Sbjct: 182 DYSYSVASKFAIFRAGKL 235
>TC78583 similar to GP|4097585|gb|AAD09518.1| NTGP4 {Nicotiana tabacum},
partial (86%)
Length = 1269
Score = 28.5 bits (62), Expect = 1.8
Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 9/53 (16%)
Frame = +3
Query: 51 LRLRFYGDDERCVVSLKA---------KAVLVDGVSRVEEDEEDLDPKIGRDC 94
+R RF ++E + SL+ V+ G +E+DE+ LD +GR+C
Sbjct: 414 VRSRFSEEEENALRSLQTLFGSKIVDYMIVVFTGGDELEDDEDTLDDYLGREC 572
>BE323735 similar to GP|21592923|gb induced upon wounding stress {Arabidopsis
thaliana}, partial (26%)
Length = 356
Score = 26.9 bits (58), Expect = 5.3
Identities = 14/30 (46%), Positives = 19/30 (62%), Gaps = 4/30 (13%)
Frame = -3
Query: 114 EEFGVVGENGFVGLG----GFKNVRNVYDW 139
E+ G +GENG GLG G+ + NV+DW
Sbjct: 354 EDVGNIGENGEFGLGVI*IGYVTL-NVFDW 268
>TC85524 similar to SP|P93508|CRTC_RICCO Calreticulin precursor. [Castor bean]
{Ricinus communis}, partial (93%)
Length = 1699
Score = 26.9 bits (58), Expect = 5.3
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +1
Query: 130 FKNVRNVYDWKGLK-LEVDETHFDFGTLFEIECESSDPEEAKRILEE 175
FK+ ++Y + LK + ++ GTLF+ + DPE AK++ EE
Sbjct: 991 FKDDPDIYVFPKLKYVGIELWQVKSGTLFDNVVITDDPEYAKQVAEE 1131
>BG645999 similar to GP|16930745|gb NADH-ubiquinone oxidoreductase {Retama
raetam}, partial (84%)
Length = 742
Score = 26.9 bits (58), Expect = 5.3
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +2
Query: 130 FKNVRNVYDWKGLK-LEVDETHFDFGTLFEIECESSDPEEAKRILEE 175
FK+ ++Y + LK + ++ GTLF+ + DPE AK++ EE
Sbjct: 539 FKDDPDIYVFPKLKYVGIELWQVKSGTLFDNVVITDDPEYAKQVAEE 679
>TC77464 similar to GP|13272433|gb|AAK17155.1 unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 1109
Score = 26.6 bits (57), Expect = 6.9
Identities = 15/29 (51%), Positives = 15/29 (51%)
Frame = +1
Query: 111 RVKEEFGVVGENGFVGLGGFKNVRNVYDW 139
RVK G VGE G GG R VYDW
Sbjct: 523 RVKCFNGAVGETGAA*GGGS*RRREVYDW 609
>TC87770 similar to GP|14970762|emb|CAC44460. protein tyrosine phosphatase
{Lycopersicon esculentum}, partial (82%)
Length = 1714
Score = 26.2 bits (56), Expect = 9.0
Identities = 15/37 (40%), Positives = 20/37 (53%)
Frame = +3
Query: 9 LPNADSYHRVTTLLSPFHVITHRQHNLFFDGAGSELS 45
LP + S + TL+SP T R HN+F + S LS
Sbjct: 303 LPRSSSVLQFHTLVSP--PSTARHHNIFLPSSSSSLS 407
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.141 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,548,860
Number of Sequences: 36976
Number of extensions: 73395
Number of successful extensions: 339
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 337
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 339
length of query: 200
length of database: 9,014,727
effective HSP length: 91
effective length of query: 109
effective length of database: 5,649,911
effective search space: 615840299
effective search space used: 615840299
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144477.10