BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144475.11 - phase: 0 /pseudo
(74 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85396 homologue to GP|6911551|emb|CAB72129.1 heat shock protei... 154 5e-39
TC85392 homologue to PIR|JC4786|JC4786 dnaK-type molecular chape... 153 1e-38
TC85390 homologue to SP|P27322|HS72_LYCES Heat shock cognate 70 ... 153 1e-38
BQ139103 homologue to GP|2655420|gb| heat shock cognate protein ... 152 2e-38
CA859549 similar to GP|20260807|gb Hsp70 protein 1 {Rhizopus sto... 134 5e-33
TC80669 homologue to PIR|S53498|S53498 dnaK-type molecular chape... 133 1e-32
BE321871 GP|20563125|db heat shock cognate protein {Bombyx mori}... 129 2e-31
TC80733 homologue to SP|Q01233|HS70_NEUCR Heat shock 70 kDa prot... 119 2e-28
BG646630 homologue to GP|21479|emb|CAA 70-Kd heat shock protein... 115 2e-27
BQ752417 similar to GP|5597020|dbj ER chaperone BiP {Aspergillus... 108 3e-25
TC80052 similar to PIR|JC4786|JC4786 dnaK-type molecular chapero... 105 4e-24
TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protei... 105 4e-24
TC85394 homologue to GP|2642238|gb|AAB86942.1| endoplasmic retic... 105 4e-24
AW695262 homologue to GP|170386|gb|A glucose-regulated protein 7... 105 4e-24
AW559598 weakly similar to SP|P09189|HS7C Heat shock cognate 70 ... 101 5e-23
TC85397 homologue to GP|170386|gb|AAA99920.1|| glucose-regulated... 92 5e-20
TC85716 homologue to PIR|S19140|S19140 dnaK-type molecular chape... 68 6e-13
TC85718 homologue to SP|Q01899|HS7M_PHAVU Heat shock 70 kDa prot... 68 8e-13
BQ138113 similar to GP|1552344|emb| heat shock protein 70 {Leish... 62 3e-11
TC77585 similar to GP|17473863|gb|AAL38353.1 putative heat-shock... 50 2e-07
>TC85396 homologue to GP|6911551|emb|CAB72129.1 heat shock protein 70
{Cucumis sativus}, complete
Length = 2320
Score = 154 bits (390), Expect = 5e-39
Identities = 74/74 (100%), Positives = 74/74 (100%)
Frame = +2
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 60
MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN
Sbjct: 107 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 286
Query: 61 QVAMNPTNTVFDAK 74
QVAMNPTNTVFDAK
Sbjct: 287 QVAMNPTNTVFDAK 328
>TC85392 homologue to PIR|JC4786|JC4786 dnaK-type molecular chaperone
hsc70-3 - tomato, complete
Length = 2295
Score = 153 bits (387), Expect = 1e-38
Identities = 73/74 (98%), Positives = 74/74 (99%)
Frame = +1
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 60
MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTD+ERLIGDAAKN
Sbjct: 130 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKN 309
Query: 61 QVAMNPTNTVFDAK 74
QVAMNPTNTVFDAK
Sbjct: 310 QVAMNPTNTVFDAK 351
>TC85390 homologue to SP|P27322|HS72_LYCES Heat shock cognate 70 kDa protein
2. [Tomato] {Lycopersicon esculentum}, complete
Length = 2278
Score = 153 bits (387), Expect = 1e-38
Identities = 73/74 (98%), Positives = 74/74 (99%)
Frame = +1
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 60
MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTD+ERLIGDAAKN
Sbjct: 136 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKN 315
Query: 61 QVAMNPTNTVFDAK 74
QVAMNPTNTVFDAK
Sbjct: 316 QVAMNPTNTVFDAK 357
>BQ139103 homologue to GP|2655420|gb| heat shock cognate protein HSC70
{Brassica napus}, partial (29%)
Length = 628
Score = 152 bits (384), Expect = 2e-38
Identities = 73/74 (98%), Positives = 73/74 (98%)
Frame = +2
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 60
MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN
Sbjct: 56 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 235
Query: 61 QVAMNPTNTVFDAK 74
QVAMNP NTVFDAK
Sbjct: 236 QVAMNPINTVFDAK 277
>CA859549 similar to GP|20260807|gb Hsp70 protein 1 {Rhizopus stolonifer},
partial (19%)
Length = 527
Score = 134 bits (338), Expect = 5e-33
Identities = 64/69 (92%), Positives = 67/69 (96%)
Frame = +3
Query: 6 EGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMN 65
EG A+GIDLGTTYSCVGVWQ+DRVEIIANDQGNRTTPSYVAFTD+ERLIGDAAKNQVAMN
Sbjct: 156 EGKAVGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 335
Query: 66 PTNTVFDAK 74
P NTVFDAK
Sbjct: 336 PYNTVFDAK 362
>TC80669 homologue to PIR|S53498|S53498 dnaK-type molecular chaperone
HSP71.2 - garden pea, partial (44%)
Length = 955
Score = 133 bits (335), Expect = 1e-32
Identities = 64/69 (92%), Positives = 66/69 (94%)
Frame = +2
Query: 6 EGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMN 65
EG AIGIDLGTTYSCVGVWQ+DRVEII NDQGNRTTPSYVAFTD+ERLIGDAAKNQVAMN
Sbjct: 110 EGKAIGIDLGTTYSCVGVWQNDRVEIIPNDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 289
Query: 66 PTNTVFDAK 74
P NTVFDAK
Sbjct: 290 PQNTVFDAK 316
>BE321871 GP|20563125|db heat shock cognate protein {Bombyx mori}, partial
(13%)
Length = 346
Score = 129 bits (324), Expect = 2e-31
Identities = 60/69 (86%), Positives = 66/69 (94%)
Frame = +1
Query: 6 EGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMN 65
+ PA+GIDLGTTYSCVGV+QH +VEIIANDQGNRTTPSYVAFTD+ERLIGDAAKNQVAMN
Sbjct: 85 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 264
Query: 66 PTNTVFDAK 74
P NT+FDAK
Sbjct: 265 PNNTIFDAK 291
>TC80733 homologue to SP|Q01233|HS70_NEUCR Heat shock 70 kDa protein
(HSP70). {Neurospora crassa}, partial (54%)
Length = 1258
Score = 119 bits (298), Expect = 2e-28
Identities = 57/66 (86%), Positives = 62/66 (93%)
Frame = +2
Query: 9 AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPTN 68
AIGIDLGTTYSCVG + +D++EIIANDQGNRTTPSYVAF D+ERLIGDAAKNQVAMNP N
Sbjct: 188 AIGIDLGTTYSCVGRYANDKIEIIANDQGNRTTPSYVAFNDTERLIGDAAKNQVAMNPHN 367
Query: 69 TVFDAK 74
TVFDAK
Sbjct: 368 TVFDAK 385
>BG646630 homologue to GP|21479|emb|CAA 70-Kd heat shock protein {Solanum
tuberosum}, partial (90%)
Length = 526
Score = 115 bits (287), Expect(2) = 2e-27
Identities = 54/55 (98%), Positives = 54/55 (98%)
Frame = +3
Query: 17 TYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPTNTVF 71
TYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNP NTVF
Sbjct: 30 TYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVF 194
Score = 22.3 bits (46), Expect(2) = 2e-27
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2
Query: 8 PAIGIDLGT 16
PAIGIDLGT
Sbjct: 2 PAIGIDLGT 28
>BQ752417 similar to GP|5597020|dbj ER chaperone BiP {Aspergillus oryzae},
partial (18%)
Length = 690
Score = 108 bits (271), Expect = 3e-25
Identities = 51/68 (75%), Positives = 56/68 (82%)
Frame = +1
Query: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNP 66
G IGIDLGTTYSCVGV Q +VEI+ NDQGNR TPSYVAFT+ ERL+GDAAKNQ A NP
Sbjct: 322 GTVIGIDLGTTYSCVGVMQGGKVEILVNDQGNRITPSYVAFTEEERLVGDAAKNQAAANP 501
Query: 67 TNTVFDAK 74
NT+FD K
Sbjct: 502 YNTIFDIK 525
>TC80052 similar to PIR|JC4786|JC4786 dnaK-type molecular chaperone hsc70-3
- tomato, partial (27%)
Length = 667
Score = 105 bits (261), Expect = 4e-24
Identities = 50/74 (67%), Positives = 60/74 (80%)
Frame = +3
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKN 60
M+G+G+ AIGIDLGTTYSCV V ++ ++II ND G RTTPS+VAF DSER+IGDAA N
Sbjct: 81 MSGRGKKVAIGIDLGTTYSCVAVCKNGEIDIIVNDLGKRTTPSFVAFKDSERMIGDAAFN 260
Query: 61 QVAMNPTNTVFDAK 74
A NPTNT+FDAK
Sbjct: 261 IAASNPTNTIFDAK 302
>TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protein 4
precursor (BiP 4) (78 kDa glucose-regulated protein
homolog 4) (GRP 78-4)., partial (94%)
Length = 2265
Score = 105 bits (261), Expect = 4e-24
Identities = 49/68 (72%), Positives = 57/68 (83%)
Frame = +2
Query: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNP 66
G IGIDLGTTYSCVGV+++ VEIIANDQGNR TPS+V+FTD ERLIG+AAKN A+NP
Sbjct: 155 GTVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVSFTDDERLIGEAAKNLAAVNP 334
Query: 67 TNTVFDAK 74
T+FD K
Sbjct: 335 ERTIFDVK 358
>TC85394 homologue to GP|2642238|gb|AAB86942.1| endoplasmic reticulum
HSC70-cognate binding protein precursor {Glycine max},
partial (38%)
Length = 962
Score = 105 bits (261), Expect = 4e-24
Identities = 49/68 (72%), Positives = 57/68 (83%)
Frame = +1
Query: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNP 66
G IGIDLGTTYSCVGV+++ VEIIANDQGNR TPS+V+FTD ERLIG+AAKN A+NP
Sbjct: 289 GTVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVSFTDDERLIGEAAKNLAAVNP 468
Query: 67 TNTVFDAK 74
T+FD K
Sbjct: 469 ERTIFDVK 492
>AW695262 homologue to GP|170386|gb|A glucose-regulated protein 78
{Lycopersicon esculentum}, partial (39%)
Length = 607
Score = 105 bits (261), Expect = 4e-24
Identities = 49/68 (72%), Positives = 57/68 (83%)
Frame = +2
Query: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNP 66
G IGIDLGTTYSCVGV+++ VEIIANDQGNR TPS+V+FTD ERLIG+AAKN A+NP
Sbjct: 128 GTVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVSFTDDERLIGEAAKNLAAVNP 307
Query: 67 TNTVFDAK 74
T+FD K
Sbjct: 308 ERTIFDVK 331
>AW559598 weakly similar to SP|P09189|HS7C Heat shock cognate 70 kDa protein.
[Petunia] {Petunia hybrida}, partial (24%)
Length = 532
Score = 101 bits (252), Expect = 5e-23
Identities = 50/76 (65%), Positives = 61/76 (79%), Gaps = 2/76 (2%)
Frame = +1
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVW--QHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAA 58
MA E A+GIDLGTTYSCV VW + +RVEII ND+G++ TPS+VAFTD +RL+G AA
Sbjct: 31 MANNCECYAVGIDLGTTYSCVAVWLEEKNRVEIIHNDEGSKITPSFVAFTDGQRLVGAAA 210
Query: 59 KNQVAMNPTNTVFDAK 74
K+Q A+NP NTVFDAK
Sbjct: 211 KDQAAINPQNTVFDAK 258
>TC85397 homologue to GP|170386|gb|AAA99920.1|| glucose-regulated protein
78 {Lycopersicon esculentum}, partial (57%)
Length = 648
Score = 91.7 bits (226), Expect = 5e-20
Identities = 42/59 (71%), Positives = 50/59 (84%)
Frame = +1
Query: 16 TTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPTNTVFDAK 74
TTYSCVGV+++ VEIIANDQGNR TPS+V+FTD ERLIG+AAKN A+NP T+FD K
Sbjct: 1 TTYSCVGVYKNGHVEIIANDQGNRITPSWVSFTDDERLIGEAAKNLAAVNPERTIFDVK 177
>TC85716 homologue to PIR|S19140|S19140 dnaK-type molecular chaperone PHSP1
precursor mitochondrial - garden pea, partial (98%)
Length = 2422
Score = 68.2 bits (165), Expect = 6e-13
Identities = 35/66 (53%), Positives = 42/66 (63%), Gaps = 1/66 (1%)
Frame = +1
Query: 10 IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTD-SERLIGDAAKNQVAMNPTN 68
IGIDLGTT SCV + + ++I N +G RTTPS VAF E L+G AK Q NPTN
Sbjct: 247 IGIDLGTTNSCVSLMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTN 426
Query: 69 TVFDAK 74
T+F K
Sbjct: 427 TLFGTK 444
>TC85718 homologue to SP|Q01899|HS7M_PHAVU Heat shock 70 kDa protein
mitochondrial precursor. [Kidney bean French bean]
{Phaseolus vulgaris}, complete
Length = 2371
Score = 67.8 bits (164), Expect = 8e-13
Identities = 35/66 (53%), Positives = 42/66 (63%), Gaps = 1/66 (1%)
Frame = +2
Query: 10 IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTD-SERLIGDAAKNQVAMNPTN 68
IGIDLGTT SCV V + +++ N +G RTTPS VAFT E L+G AK Q NP N
Sbjct: 263 IGIDLGTTNSCVSVMEGKNPKVVENSEGARTTPSVVAFTQKGELLVGTPAKRQAVTNPEN 442
Query: 69 TVFDAK 74
T+ AK
Sbjct: 443 TISGAK 460
>BQ138113 similar to GP|1552344|emb| heat shock protein 70 {Leishmania
infantum}, partial (6%)
Length = 317
Score = 62.4 bits (150), Expect = 3e-11
Identities = 39/76 (51%), Positives = 48/76 (62%), Gaps = 2/76 (2%)
Frame = +3
Query: 1 MAGKGEGPAIGIDLGTTYSCVGVW--QHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAA 58
M G E AIGIDLGTT SCV V+ HDRV+II TT S V+FTD+E++IG +
Sbjct: 66 MTGNCENYAIGIDLGTTRSCVAVYMPDHDRVDIIG------TTLSSVSFTDTEKIIGGFS 227
Query: 59 KNQVAMNPTNTVFDAK 74
+ N T TV+DAK
Sbjct: 228 R-----NATKTVYDAK 260
>TC77585 similar to GP|17473863|gb|AAL38353.1 putative heat-shock protein
{Arabidopsis thaliana}, partial (92%)
Length = 3027
Score = 49.7 bits (117), Expect = 2e-07
Identities = 22/65 (33%), Positives = 33/65 (49%)
Frame = +1
Query: 10 IGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPTNT 69
+G D G V V + ++++ ND+ R TP+ V F D +R IG A MNP N+
Sbjct: 247 VGFDFGNESCIVAVARQRGIDVVLNDESKRETPAIVCFGDKQRFIGTAGAASTMMNPKNS 426
Query: 70 VFDAK 74
+ K
Sbjct: 427 ISQIK 441
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.132 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,704,925
Number of Sequences: 36976
Number of extensions: 14386
Number of successful extensions: 85
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 82
length of query: 74
length of database: 9,014,727
effective HSP length: 50
effective length of query: 24
effective length of database: 7,165,927
effective search space: 171982248
effective search space used: 171982248
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 51 (24.3 bits)
Medicago: description of AC144475.11