BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144474.7 - phase: 0
(329 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83492 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3... 368 e-102
TC82482 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3... 187 4e-48
TC82954 weakly similar to GP|8809591|dbj|BAA97142.1 emb|CAB82814... 37 0.011
TC90246 similar to PIR|T00872|T00872 probable protein kinase At2... 33 0.15
AW694651 similar to PIR|T47659|T4 spliceosomal-like protein - Ar... 32 0.26
AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - A... 32 0.44
TC86965 similar to GP|22022558|gb|AAM83236.1 At5g12410 {Arabidop... 32 0.44
TC87926 weakly similar to PIR|F86184|F86184 hypothetical protein... 31 0.76
TC86635 weakly similar to GP|9758600|dbj|BAB09233.1 gene_id:MDF2... 30 1.3
TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I... 30 1.3
TC76440 weakly similar to SP|Q9LD55|IF3A_ARATH Eukaryotic transl... 29 2.2
TC84400 similar to GP|2149820|gb|AAB58660.1| maturase {Nothofagu... 29 2.2
BI268261 similar to SP|P56425|MB34_ Antibacterial peptide BMAP-3... 29 2.9
TC82261 similar to GP|10177249|dbj|BAB10717. contains similarity... 28 3.8
BG448516 weakly similar to PIR|T45706|T4 chromosome-associated p... 28 3.8
BE204862 similar to GP|20197280|gb putative splicing factor {Ara... 28 6.4
TC82483 weakly similar to GP|13699910|dbj|BAB41214. putative tra... 28 6.4
BM779021 homologue to PIR|T05883|T058 ATP-dependent helicase F6H... 28 6.4
TC79228 weakly similar to GP|9971154|dbj|BAB12429.1 mkpA {Dictyo... 28 6.4
TC93373 similar to GP|3676071|emb|CAA76979.1 ERG protein {Arabid... 27 8.4
>TC83492 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3L24_8
{Arabidopsis thaliana}, partial (42%)
Length = 562
Score = 368 bits (944), Expect = e-102
Identities = 179/181 (98%), Positives = 179/181 (98%)
Frame = +3
Query: 1 MKGMLTLHLWNPPPRFYTPSLSTKPPLSISATSSPELLTAKERRRLRNERRESNATNWKE 60
MKGMLTLHLWNPPPRFYTPSLSTKPPLSI ATSSPELLTAKERRRLRNERRESNATNWKE
Sbjct: 18 MKGMLTLHLWNPPPRFYTPSLSTKPPLSIFATSSPELLTAKERRRLRNERRESNATNWKE 197
Query: 61 EVENKLIQKTKKVNKSWKDELNLDNLMKLGPQWWGVRVSRVKGQYTAEALARSLAKFFPD 120
EVENKLIQKTKK NKSWKDELNLDNLMKLGPQWWGVRVSRVKGQYTAEALARSLAKFFPD
Sbjct: 198 EVENKLIQKTKKGNKSWKDELNLDNLMKLGPQWWGVRVSRVKGQYTAEALARSLAKFFPD 377
Query: 121 IEFKVYAPAIHEKKRLKSGSISVKSKPLFPGCIFLRCELNKPLHDYIKEYEGVGGFIGSK 180
IEFKVYAPAIHEKKRLKSGSISVKSKPLFPGCIFLRCELNKPLHDYIKEYEGVGGFIGSK
Sbjct: 378 IEFKVYAPAIHEKKRLKSGSISVKSKPLFPGCIFLRCELNKPLHDYIKEYEGVGGFIGSK 557
Query: 181 V 181
V
Sbjct: 558 V 560
>TC82482 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3L24_8
{Arabidopsis thaliana}, partial (17%)
Length = 672
Score = 187 bits (476), Expect = 4e-48
Identities = 98/101 (97%), Positives = 98/101 (97%)
Frame = +3
Query: 229 GNPNKELESDVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPKLVTGSTVQIISGSFL 288
G P KELESDVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPKLVTGSTVQII GSFL
Sbjct: 3 GIPYKELESDVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPKLVTGSTVQIIFGSFL 182
Query: 289 GFAGTLKKLNSKTKMATVHFTMFGKENIVDLDVSEIVPETN 329
GFAGTLKKLNSKTKMATVHFTMFGKENIVDLDVSEIVPETN
Sbjct: 183 GFAGTLKKLNSKTKMATVHFTMFGKENIVDLDVSEIVPETN 305
>TC82954 weakly similar to GP|8809591|dbj|BAA97142.1
emb|CAB82814.1~gene_id:MNB8.8~similar to unknown protein
{Arabidopsis thaliana}, partial (7%)
Length = 783
Score = 37.0 bits (84), Expect = 0.011
Identities = 29/88 (32%), Positives = 38/88 (42%)
Frame = +3
Query: 183 NTKKQINKPRPVAEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKA 242
N + K R +A D+ K+ + E DK EEEEKK KELE KA
Sbjct: 444 NELRVFEKKRDLAISDLNQKLKEMEGLVEEKDKKIEEEEKK--------RKELEEKAKKA 599
Query: 243 IVDSKPKRGSRKTSNQLTITEEASSAKK 270
D++ R S K Q E +S +K
Sbjct: 600 EKDAEELRESSKREGQ----EHSSDLRK 671
>TC90246 similar to PIR|T00872|T00872 probable protein kinase At2g45590
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1451
Score = 33.1 bits (74), Expect = 0.15
Identities = 17/49 (34%), Positives = 26/49 (52%)
Frame = +2
Query: 46 LRNERRESNATNWKEEVENKLIQKTKKVNKSWKDELNLDNLMKLGPQWW 94
+R E+R+S WKEE ++ ++ KK K K N D+ + G WW
Sbjct: 350 MRKEKRKSVREWWKEEHCEEVAKEKKKNKKKKKGVENGDDGISNGDNWW 496
>AW694651 similar to PIR|T47659|T4 spliceosomal-like protein - Arabidopsis
thaliana, partial (11%)
Length = 503
Score = 32.3 bits (72), Expect = 0.26
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 4 MLTLHLWNPPPRFYTPSLSTKPPLSISATSSPE 36
+ T+ L+N PP PS +T P + + +SSPE
Sbjct: 104 IFTISLFNDPPESSVPSTATSPAVRVKKSSSPE 202
>AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - African
clawed frog, partial (5%)
Length = 516
Score = 31.6 bits (70), Expect = 0.44
Identities = 25/90 (27%), Positives = 45/90 (49%), Gaps = 3/90 (3%)
Frame = +3
Query: 185 KKQINKPRPV-AEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKAI 243
KK+ KP P+ E+D + + + E E K +K AA + +E S S++
Sbjct: 141 KKKEEKPAPMEVEEDSSSSEESSSSEDEAPAKKAAPAKKAAAKKESSSEEESSSSESESE 320
Query: 244 VDSKP--KRGSRKTSNQLTITEEASSAKKK 271
+ KP K ++K S+ EE+SS++++
Sbjct: 321 EEEKPAKKAAAKKESSS---EEESSSSEEE 401
>TC86965 similar to GP|22022558|gb|AAM83236.1 At5g12410 {Arabidopsis
thaliana}, partial (59%)
Length = 1455
Score = 31.6 bits (70), Expect = 0.44
Identities = 25/122 (20%), Positives = 54/122 (43%), Gaps = 17/122 (13%)
Frame = +1
Query: 171 EGVGGFIGSKVGNTKKQINKPRPVAE--DDMEAIFK------------QAKVEQENADKA 216
+G+ G K + K ++ P+ + + D++ A+ + + KVE+++ DK
Sbjct: 493 DGLHAQAGDKANDDKGDVDSPKTIEKIADELPAVKECCKTTAPTSNLGEKKVEEKSIDKL 672
Query: 217 FEEEEKKAAVNSGNPNKELESDVSKAIVDSKPKRGSRKTSNQLT---ITEEASSAKKKPK 273
E+E + S +LES + + K+ K+ N++ +T AS+ K +
Sbjct: 673 IEDELVELRDKSKKRFAKLESGCNGVVFIQMRKKDGDKSPNKIVNRIVTSAASTRKHMSR 852
Query: 274 LV 275
+
Sbjct: 853 FI 858
>TC87926 weakly similar to PIR|F86184|F86184 hypothetical protein [imported]
- Arabidopsis thaliana, partial (71%)
Length = 931
Score = 30.8 bits (68), Expect = 0.76
Identities = 16/43 (37%), Positives = 25/43 (57%)
Frame = +3
Query: 220 EEKKAAVNSGNPNKELESDVSKAIVDSKPKRGSRKTSNQLTIT 262
+EKK ++S NPNK L++ + + I KP TS ++T T
Sbjct: 51 QEKKLLISSKNPNKPLQN*LKRTIQSPKPTLLINGTSFKITPT 179
>TC86635 weakly similar to GP|9758600|dbj|BAB09233.1
gene_id:MDF20.10~pir||T08929~similar to unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 1963
Score = 30.0 bits (66), Expect = 1.3
Identities = 18/64 (28%), Positives = 32/64 (49%)
Frame = +2
Query: 12 PPPRFYTPSLSTKPPLSISATSSPELLTAKERRRLRNERRESNATNWKEEVENKLIQKTK 71
PP R S +T+P SP++ + K++ +++ S T K ++K +KT+
Sbjct: 1145 PPKRAPKKSSNTRPKSDDDINESPKVFSRKKKSEQGGKQKISTPTPTKSSSKSK--EKTE 1318
Query: 72 KVNK 75
KV K
Sbjct: 1319 KVTK 1330
>TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I3.230 -
Arabidopsis thaliana, partial (14%)
Length = 1074
Score = 30.0 bits (66), Expect = 1.3
Identities = 24/84 (28%), Positives = 34/84 (39%)
Frame = +3
Query: 190 KPRPVAEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKAIVDSKPK 249
K VAE D E K KV+ + +++EKK N SD K + K K
Sbjct: 297 KKSKVAEVDGEEKVKTKKVDDAAVEVEDDKKEKKKKKKKDKENGAAASDEEK-VEKEKKK 473
Query: 250 RGSRKTSNQLTITEEASSAKKKPK 273
+ K + E++ KKK K
Sbjct: 474 KHKEKGEDGSPEVEKSDKKKKKHK 545
>TC76440 weakly similar to SP|Q9LD55|IF3A_ARATH Eukaryotic translation
initiation factor 3 subunit 10 (eIF-3 theta), partial
(41%)
Length = 1953
Score = 29.3 bits (64), Expect = 2.2
Identities = 57/270 (21%), Positives = 109/270 (40%), Gaps = 31/270 (11%)
Frame = +1
Query: 37 LLTAKERRRLRNERRESNATNWKEEVENKLIQKTKKVNKSWKD------ELNLDNLMKLG 90
L T E+R+++ +RE + +EE E ++ K++ + K +++ ++LM+L
Sbjct: 541 LATEIEQRKIQRLQREKEERD-REEAEALRLEAEKRLKRKGKKPVIEGGQISRESLMQL- 714
Query: 91 PQWWGVRVSRVKGQYTAEALARSLAKFFPDIEF---KVYAPAIHEKKRLKSGSISVKSKP 147
V +V+ + E + LAK +E + AP I + + V+ +
Sbjct: 715 -----TLVEQVRERQEMEKKLQKLAKTMDHLERAKREEAAPLIDAAYQQRL----VEERV 867
Query: 148 LFPGCIFLRCELNKPLH-DYIKEYEGVGGFIGSK-------VGNTKKQINK--------- 190
L L+ EL++ H + E E + +G+K V + + + N+
Sbjct: 868 LHEREQQLQVELSRQRHAGDLNEKERLSRMMGNKEIYQERVVSHRQAEFNRLQRDRLDRI 1047
Query: 191 -----PRPVAEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKAIVD 245
R + M + KVE+E K EEEE + + KE +A
Sbjct: 1048SKILLSRKQEREKMRKLKYYLKVEEEKRQKLREEEEAREREEAERKKKE------QAERQ 1209
Query: 246 SKPKRGSRKTSNQLTITEEASSAKKKPKLV 275
+K + K +L EE + +K+ L+
Sbjct: 1210AKLDEIAEKQKQRLREIEEKAEREKREALL 1299
>TC84400 similar to GP|2149820|gb|AAB58660.1| maturase {Nothofagus
cunninghamii}, partial (12%)
Length = 671
Score = 29.3 bits (64), Expect = 2.2
Identities = 24/72 (33%), Positives = 32/72 (44%)
Frame = -2
Query: 183 NTKKQINKPRPVAEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKA 242
N KK +K P E+ + K +Q + K EEEKK N +P+KE E K
Sbjct: 193 NKKKGTSKHSPSKEEG-----EHTKSKQHSPSK---EEEKKTKSNQHSPSKEEE----KC 50
Query: 243 IVDSKPKRGSRK 254
I P +G K
Sbjct: 49 INMHSPNKGEEK 14
>BI268261 similar to SP|P56425|MB34_ Antibacterial peptide BMAP-34 precursor.
[Bovine] {Bos taurus}, partial (8%)
Length = 500
Score = 28.9 bits (63), Expect = 2.9
Identities = 18/57 (31%), Positives = 27/57 (46%), Gaps = 2/57 (3%)
Frame = +3
Query: 64 NKLIQKTKKVNKSWKD--ELNLDNLMKLGPQWWGVRVSRVKGQYTAEALARSLAKFF 118
N+ +TK++ K KD EL L + QWW + + Q EAL + +FF
Sbjct: 264 NRSPNETKRIIKQMKDGKELVLKCSNEDKIQWWNCWYKKAQXQEELEALDEKIKRFF 434
>TC82261 similar to GP|10177249|dbj|BAB10717. contains similarity to
phytocyanin/early nodulin-like protein~gene_id:K19P17.3,
partial (7%)
Length = 740
Score = 28.5 bits (62), Expect = 3.8
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1
Query: 14 PRFYTPSLSTKPPLSISATSSP 35
P F+ PSL T P LS S +SSP
Sbjct: 460 PSFFLPSLLTSPELSPSPSSSP 525
>BG448516 weakly similar to PIR|T45706|T4 chromosome-associated protein E
homolog F1P2.10 - Arabidopsis thaliana, partial (9%)
Length = 351
Score = 28.5 bits (62), Expect = 3.8
Identities = 21/76 (27%), Positives = 33/76 (42%), Gaps = 6/76 (7%)
Frame = +1
Query: 193 PVAEDDMEAIFKQAKVEQENADKAFEEEEKKAAVNSG------NPNKELESDVSKAIVDS 246
P ED+MEA+ K+ + E++ K +E +A S +P K + K +V
Sbjct: 97 PYKEDEMEALQKERESERDCVQKLKDEIRNLSAYLSNVEFSYIDPVKNFDRSKVKGVVAK 276
Query: 247 KPKRGSRKTSNQLTIT 262
K R T L +T
Sbjct: 277 LIKVKDRSTVTALEVT 324
>BE204862 similar to GP|20197280|gb putative splicing factor {Arabidopsis
thaliana}, partial (20%)
Length = 588
Score = 27.7 bits (60), Expect = 6.4
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = -1
Query: 3 GMLTLHLWNPPPRFYTPSL---STKPPLSISATSSPELLTAK 41
G++++ +W PPP TP +T L I+ P LLT +
Sbjct: 330 GLVSIGIWRPPPGIPTPGAGIRATAGMLPIACRLRPVLLTTE 205
>TC82483 weakly similar to GP|13699910|dbj|BAB41214. putative
transcriptional coactivator {Brassica rapa}, partial
(79%)
Length = 938
Score = 27.7 bits (60), Expect = 6.4
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +2
Query: 199 MEAIFKQAKVEQENADKAFEEEEKKAAVNSGNPNKELESDVSKAIV 244
+E Q + EQ+N K EEEEK+ G N+ D S +V
Sbjct: 200 IEDFLLQKQQEQQNEKKEEEEEEKQQVKQEGASNRSTVYDDSGDLV 337
>BM779021 homologue to PIR|T05883|T058 ATP-dependent helicase F6H11.20 -
Arabidopsis thaliana, partial (9%)
Length = 492
Score = 27.7 bits (60), Expect = 6.4
Identities = 14/43 (32%), Positives = 23/43 (52%), Gaps = 3/43 (6%)
Frame = +1
Query: 39 TAKERRRLRNERRESNATNWKEEVEN---KLIQKTKKVNKSWK 78
T RRR+R+ RE + NW + ++N + I KT+ + K
Sbjct: 46 TTVTRRRIRSFAREKDLVNWNKNLKNNKRRRIMKTQNLKMKKK 174
>TC79228 weakly similar to GP|9971154|dbj|BAB12429.1 mkpA {Dictyostelium
discoideum}, partial (2%)
Length = 965
Score = 27.7 bits (60), Expect = 6.4
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = -1
Query: 161 KPLHDYIKEYEGVGGFIGSKVGNTKKQINKPRPVAEDDMEAIFKQAKVEQENAD 214
KPL + +KE +GVG GS ++K NK + E+ + K+ + + + AD
Sbjct: 665 KPLSEILKEKKGVGAGAGSS-NSSKHDDNKNEGITENGTLSESKEEESKIQAAD 507
>TC93373 similar to GP|3676071|emb|CAA76979.1 ERG protein {Arabidopsis
thaliana}, partial (4%)
Length = 700
Score = 27.3 bits (59), Expect = 8.4
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +3
Query: 12 PPPRFYTPSLSTKPPLSISATSSPELLTAKERRRLRNERR-ESNATNW 58
P P P++ T+P + + T S L+T RR R R E+ NW
Sbjct: 447 PNPSHRNPNIQTQPSI-VLTTKSQSLITTTXSRR*RGIRNIETERINW 587
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.129 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,897,998
Number of Sequences: 36976
Number of extensions: 114436
Number of successful extensions: 810
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 800
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of query: 329
length of database: 9,014,727
effective HSP length: 96
effective length of query: 233
effective length of database: 5,465,031
effective search space: 1273352223
effective search space used: 1273352223
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144474.7