BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144474.2 - phase: 0
(201 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81542 similar to GP|13877685|gb|AAK43920.1 Unknown protein {Ar... 285 1e-77
TC81132 similar to GP|20466560|gb|AAM20597.1 unknown protein {Ar... 53 7e-08
TC84063 similar to GP|7339697|dbj|BAA92902.1 hypothetical protei... 53 9e-08
BG586394 similar to GP|7295463|gb| CG4835 gene product {Drosophi... 31 0.37
TC89979 similar to GP|17979351|gb|AAL49901.1 unknown protein {Ar... 30 0.82
TC90133 similar to GP|13123673|gb|AAK12939.1 molybdenum cofactor... 30 0.82
TC85916 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55 ... 29 1.4
TC85917 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55 ... 28 3.1
TC86313 similar to GP|4995890|emb|CAB44316.1 serine palmitoyltra... 27 4.1
TC85057 similar to PIR|G96655|G96655 unknown protein 29405-2728... 27 4.1
TC85190 similar to GP|8894548|emb|CAB95829.1 hypothetical protei... 27 5.3
AW686640 27 5.3
AL389283 similar to GP|6934266|gb|A cytochrome oxidase subunit I... 27 7.0
TC84283 similar to GP|8777419|dbj|BAA97009.1 mitotic checkpoint ... 27 7.0
TC82190 weakly similar to GP|13937297|gb|AAK50128.1 unknown prot... 27 7.0
TC81825 weakly similar to SP|Q9FIC8|GL1G_ARATH Germin-like prote... 27 7.0
TC93095 similar to GP|17473811|gb|AAL38336.1 unknown protein {Ar... 26 9.1
>TC81542 similar to GP|13877685|gb|AAK43920.1 Unknown protein {Arabidopsis
thaliana}, partial (89%)
Length = 753
Score = 285 bits (728), Expect = 1e-77
Identities = 147/196 (75%), Positives = 161/196 (82%), Gaps = 5/196 (2%)
Frame = +3
Query: 11 ETPKYEILKTTQNYVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTKT 70
ETPKYE+ KTTQ+Y IR YAPS+ AE+TYDPS FKG+KDGGF VL +YIG G PQNTK
Sbjct: 6 ETPKYEVTKTTQDYEIRMYAPSVAAEVTYDPSQFKGNKDGGFMVLANYIGALGNPQNTKP 185
Query: 71 EKISMTTPVITKENKSSSEKIAMTVPVVTN-----EKNKMVTMQFTLPSMYLKVEEVPKP 125
EKI+MT PVITK S+EKIAMT PVVT E+NKMVTMQF LPS Y K EE PKP
Sbjct: 186 EKIAMTAPVITK---GSAEKIAMTAPVVTKSSEEGERNKMVTMQFILPSSYEKAEEAPKP 356
Query: 126 IDERVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKVIGDFLLGRYNPPA 185
DERVVIREEG +KYGVV F GVASDEVVKEKVEKLRL LE+DGFKVIGDFLLGRYNPP
Sbjct: 357 TDERVVIREEGERKYGVVKFSGVASDEVVKEKVEKLRLSLERDGFKVIGDFLLGRYNPP- 533
Query: 186 ITIPMFRTNEVLIPVE 201
T+PMFRTNEV+IP+E
Sbjct: 534 WTLPMFRTNEVMIPIE 581
>TC81132 similar to GP|20466560|gb|AAM20597.1 unknown protein {Arabidopsis
thaliana}, partial (30%)
Length = 691
Score = 53.1 bits (126), Expect = 7e-08
Identities = 35/87 (40%), Positives = 47/87 (53%), Gaps = 3/87 (3%)
Frame = +1
Query: 11 ETPKYEILKTTQNYVIRKYAPSLVAEITYDPST---FKGDKDGGFKVLVDYIGIFGKPQN 67
ET +++L Y IR+ P VAE T + F+G F VL +Y+ FGK N
Sbjct: 445 ETVNFKVLTIRDQYEIREIEPYFVAETTMPGKSGFDFRGSSQS-FNVLAEYL--FGK--N 609
Query: 68 TKTEKISMTTPVITKENKSSSEKIAMT 94
TK EK+ MTTPV T + +S K+ MT
Sbjct: 610 TKKEKMEMTTPVFTTKKQSDGVKMDMT 690
>TC84063 similar to GP|7339697|dbj|BAA92902.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (42%)
Length = 599
Score = 52.8 bits (125), Expect = 9e-08
Identities = 52/191 (27%), Positives = 88/191 (45%), Gaps = 7/191 (3%)
Frame = +2
Query: 1 MGLVFGRFSAETPKYEILKTTQNYVIRKYAPSL-VAEITYDPSTFKGDKDGGFKVLVDYI 59
M ++ + E+P+Y ++ T ++ IR Y S+ ++ + +F+ GF L +I
Sbjct: 50 MAMIRNTTATESPQYTVVHTESDFEIRLYRTSVWMSAPAVNLISFEKATRNGFHRLFQFI 229
Query: 60 GIFGKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKV 119
+ N +I MTTPV+T TVP +++ + F LP+ +
Sbjct: 230 ----QGANLNFSRIPMTTPVLT-----------TTVPGTGPLESQGYYVSFYLPTKF--Q 358
Query: 120 EEVPKPIDERVVIREEGGKKYGVVT--FGGVASDEVVKEKVEKLRLCLEKDG----FKVI 173
E P P+ E + I+ G + + V F G A DE V ++ EKL L L + G K
Sbjct: 359 ENPPLPLPE-LNIKSYGFESHCVAVRGFSGFAKDEKVVKEAEKLDLSLSRWGGESEVKRK 535
Query: 174 GDFLLGRYNPP 184
G + + +YN P
Sbjct: 536 GGYSIAQYNGP 568
>BG586394 similar to GP|7295463|gb| CG4835 gene product {Drosophila
melanogaster}, partial (2%)
Length = 734
Score = 30.8 bits (68), Expect = 0.37
Identities = 30/123 (24%), Positives = 50/123 (40%), Gaps = 2/123 (1%)
Frame = +1
Query: 9 SAETPKYEILKTTQNYVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNT 68
S ETP + K T + A + T P+ + D L + G +
Sbjct: 226 SEETPTEKPEKETTTKTTKPEAETTTEPETNTPTKSETDVPETTTTLEKNQNVPGSTTES 405
Query: 69 KTEKISMTTPVITKENKSSSEKIAMTVPVVT--NEKNKMVTMQFTLPSMYLKVEEVPKPI 126
+TE + + T ++ +EK +P T +EKNK T + T+P++ EE P
Sbjct: 406 ETETPTKSETETTTTEETPTEKPETDIPKTTTASEKNKTTTTEPTVPTV---SEETPTEK 576
Query: 127 DER 129
E+
Sbjct: 577 PEK 585
>TC89979 similar to GP|17979351|gb|AAL49901.1 unknown protein {Arabidopsis
thaliana}, partial (87%)
Length = 867
Score = 29.6 bits (65), Expect = 0.82
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +3
Query: 88 SEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGKKYG-VVTFG 146
SE + +T+P EK + F + S +L + VPKPI+ + + E+ K YG V F
Sbjct: 300 SENLFLTLP--DEEKRLWHSHLFEVKSGFLFMPNVPKPIEHKDM--EKVCKTYGKVYHFW 467
Query: 147 GVASDEVVKEKVEKLRLCLEKDG 169
V + + + +L + L +DG
Sbjct: 468 QVDRGDKLPLGIPQLMMALTRDG 536
>TC90133 similar to GP|13123673|gb|AAK12939.1 molybdenum cofactor sulfurase
{Arabidopsis thaliana}, partial (2%)
Length = 487
Score = 29.6 bits (65), Expect = 0.82
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = +3
Query: 17 ILKTTQNYVIRKYAPSLVAEITYDPSTFK 45
ILKT QN+V++ Y P+ E+ + S K
Sbjct: 162 ILKTNQNHVLQDYLPTAFEEVEFSASMIK 248
>TC85916 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55
protein-like {Arabidopsis thaliana}, partial (95%)
Length = 1807
Score = 28.9 bits (63), Expect = 1.4
Identities = 16/54 (29%), Positives = 32/54 (58%), Gaps = 2/54 (3%)
Frame = +2
Query: 119 VEEVPKPIDERVVIREEGG--KKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGF 170
V+++ K +E+ +I E ++ V TFG +A E + +E++RLCL++ +
Sbjct: 392 VKKLAKIKEEQGLIAEAADLMQEIAVETFGAMAKTEKIAFILEQVRLCLDRQDY 553
>TC85917 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55
protein-like {Arabidopsis thaliana}, partial (40%)
Length = 656
Score = 27.7 bits (60), Expect = 3.1
Identities = 16/50 (32%), Positives = 28/50 (56%)
Frame = +3
Query: 118 KVEEVPKPIDERVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEK 167
K++EV + E + +E V TFG +A E + +E++RLCL++
Sbjct: 381 KIKEVHGLLAEAADLMQE----IAVETFGAMAKTEKIAFILEQVRLCLDR 518
>TC86313 similar to GP|4995890|emb|CAB44316.1 serine palmitoyltransferase
{Solanum tuberosum}, partial (98%)
Length = 2052
Score = 27.3 bits (59), Expect = 4.1
Identities = 33/156 (21%), Positives = 61/156 (38%), Gaps = 14/156 (8%)
Frame = +2
Query: 59 IGIFGKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLK 118
IG GK E + + T I + ++ + K + +++T P+ +L
Sbjct: 1055 IGAVGKTGRGVCELLGVDTADIDIMMGTFTKSFGSCGGYIAGSKELIEYLKYTCPA-HLY 1231
Query: 119 VEEVPKPIDE------RVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKV 172
+ P + +V++ E+G + G ++E R L+K GF+V
Sbjct: 1232 ATSISPPAAQQIISSIKVILGEDGSSR-------GAQKLAKIRENSNFFRSELQKMGFEV 1390
Query: 173 IGD-----FLLGRYNP---PAITIPMFRTNEVLIPV 200
+GD + YNP PA + R N ++ V
Sbjct: 1391 LGDNDSPVMPIMLYNPAKIPAFSRECLRQNVAVVTV 1498
>TC85057 similar to PIR|G96655|G96655 unknown protein 29405-27288
[imported] - Arabidopsis thaliana, partial (31%)
Length = 546
Score = 27.3 bits (59), Expect = 4.1
Identities = 14/44 (31%), Positives = 24/44 (53%), Gaps = 4/44 (9%)
Frame = -3
Query: 70 TEKISMTTPVITKENKSSSEKIAMTV----PVVTNEKNKMVTMQ 109
T I+MTT ITK K S + ++T+ + N +NK + ++
Sbjct: 349 THVINMTTNEITKSKKESDDTTSLTINYHRSITKNHRNKKMKIR 218
>TC85190 similar to GP|8894548|emb|CAB95829.1 hypothetical protein {Cicer
arietinum}, partial (50%)
Length = 1496
Score = 26.9 bits (58), Expect = 5.3
Identities = 27/95 (28%), Positives = 39/95 (40%)
Frame = +3
Query: 79 VITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVIREEGGK 138
V KE K E T VV +K++ V + + E PK ++ +EE K
Sbjct: 543 VAVKEEKKPEEDEKKTEEVVEEKKDEAVVEEKKVDEEKGSTSEEPK-VETAEPEKEE--K 713
Query: 139 KYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKVI 173
K +E V E VEK+ E+DG K +
Sbjct: 714 KV----------EETVVEVVEKIAASTEEDGAKTV 788
>AW686640
Length = 518
Score = 26.9 bits (58), Expect = 5.3
Identities = 17/59 (28%), Positives = 29/59 (48%), Gaps = 4/59 (6%)
Frame = +1
Query: 87 SSEKIAMTVPVVTNEKNKMVTMQF----TLPSMYLKVEEVPKPIDERVVIREEGGKKYG 141
+S + T P++ N + + +F T PSM +D++V+ +EGGKK G
Sbjct: 304 ASPRHKKTAPLLKNHHQEEIEQKFHKKKTTPSMRELFSS--SSLDKQVLYHDEGGKKVG 474
>AL389283 similar to GP|6934266|gb|A cytochrome oxidase subunit II
{Helobdella robusta}, partial (32%)
Length = 382
Score = 26.6 bits (57), Expect = 7.0
Identities = 17/71 (23%), Positives = 35/71 (48%)
Frame = +3
Query: 62 FGKPQNTKTEKISMTTPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYLKVEE 121
+ + +N K + + +I N S+ I +PV +K V T+PS+ +KV+
Sbjct: 72 YSEIENLKFNSMIIKNNLIRLMNTSNHLIIPRIIPVQMLISSKDVIHS*TIPSLGVKVDA 251
Query: 122 VPKPIDERVVI 132
+P +++ +I
Sbjct: 252 IPGRMNQCSII 284
>TC84283 similar to GP|8777419|dbj|BAA97009.1 mitotic checkpoint
protein-like {Arabidopsis thaliana}, partial (21%)
Length = 829
Score = 26.6 bits (57), Expect = 7.0
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 4/34 (11%)
Frame = +2
Query: 76 TTPVITKEN----KSSSEKIAMTVPVVTNEKNKM 105
T V+ KE KS ++I +T+ VVT E+NK+
Sbjct: 8 TEAVLAKEKAEVLKSEIKRIELTLAVVTEERNKL 109
>TC82190 weakly similar to GP|13937297|gb|AAK50128.1 unknown protein {Oryza
sativa}, partial (22%)
Length = 1001
Score = 26.6 bits (57), Expect = 7.0
Identities = 16/59 (27%), Positives = 31/59 (52%), Gaps = 5/59 (8%)
Frame = -3
Query: 79 VITKENKSSSEKIAMTVPVVT-----NEKNKMVTMQFTLPSMYLKVEEVPKPIDERVVI 132
+I K ++ SE A +VP++ KN++ QF+ S+ + ++ P+D+ V I
Sbjct: 414 LIDKSFENFSETTAASVPLILIWYPDRLKNRVACSQFSAESIRSRTLKIASPLDKYVSI 238
>TC81825 weakly similar to SP|Q9FIC8|GL1G_ARATH Germin-like protein
subfamily 1 member 16 precursor. [Mouse-ear cress]
{Arabidopsis thaliana}, partial (76%)
Length = 1042
Score = 26.6 bits (57), Expect = 7.0
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = -3
Query: 54 VLVDYIGIFGKPQNTKTEKISMTTP-VITKENK---SSSEKIAMTVPVVTNEKNKM 105
++ YI GKP NTK + I P I K NK ++KI+ T P N+ +K+
Sbjct: 977 LITKYIN*HGKPLNTKFKTIDSKIPSKIWKTNK**NIQTKKISNTHPK*LNKLSKV 810
>TC93095 similar to GP|17473811|gb|AAL38336.1 unknown protein {Arabidopsis
thaliana}, partial (42%)
Length = 678
Score = 26.2 bits (56), Expect = 9.1
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +3
Query: 11 ETPKYEILKTTQNY 24
E+PKY+ILK T NY
Sbjct: 627 ESPKYQILKRTANY 668
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.137 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,734,969
Number of Sequences: 36976
Number of extensions: 53172
Number of successful extensions: 294
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 289
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 289
length of query: 201
length of database: 9,014,727
effective HSP length: 91
effective length of query: 110
effective length of database: 5,649,911
effective search space: 621490210
effective search space used: 621490210
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144474.2