
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144431.4 - phase: 0 /pseudo
(1510 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93756 similar to GP|20160860|dbj|BAB89799. putative DNA polyme... 92 2e-18
TC79612 similar to SP|O04979|LON1_SPIOL Lon protease homolog 1 ... 32 1.4
AW171629 weakly similar to GP|14718995|gb| ceramide glucosyltran... 32 1.9
TC92154 32 1.9
TC78189 homologue to GP|14485513|emb|CAC42087. putative NAC doma... 32 2.4
TC78638 similar to GP|22087278|gb|AAM90909.1 p44/SSL1-like prote... 32 2.4
TC77900 similar to GP|9759182|dbj|BAB09797.1 ATP-dependent Clp p... 30 5.4
TC82248 similar to GP|15216028|emb|CAC51424. amino acid permease... 30 9.2
TC82271 similar to GP|18491137|gb|AAL69537.1 AT5g45420/MFC19_9 {... 26 9.5
>TC93756 similar to GP|20160860|dbj|BAB89799. putative DNA polymerase alpha
catalytic subunit {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 408
Score = 92.0 bits (227), Expect = 2e-18
Identities = 46/46 (100%), Positives = 46/46 (100%)
Frame = +2
Query: 1465 EAKSRIPIEKELIKIRPIVDLAASTIQKIRDRCAFGWVKLQDLVVA 1510
EAKSRIPIEKELIKIRPIVDLAASTIQKIRDRCAFGWVKLQDLVVA
Sbjct: 2 EAKSRIPIEKELIKIRPIVDLAASTIQKIRDRCAFGWVKLQDLVVA 139
>TC79612 similar to SP|O04979|LON1_SPIOL Lon protease homolog 1
mitochondrial precursor (EC 3.4.21.-). [Spinach]
{Spinacia oleracea}, partial (19%)
Length = 768
Score = 32.3 bits (72), Expect = 1.4
Identities = 26/103 (25%), Positives = 47/103 (45%), Gaps = 10/103 (9%)
Frame = -3
Query: 567 SKITLVNPPVVVTAI-----NLKTTINEKQNINEIVSASVVSCNMVKIDTPMLASEWKRP 621
SK T V PPV+ ++ +N + NI + S S S +++ I + + W P
Sbjct: 763 SKRTFVLPPVLCXCSRTEISSMAVALN*RDNIMKSGSCSTCSISVLVISREDILAVWYVP 584
Query: 622 GMLTHFTV-----IRKLDGNIFPMGFNTEVTDRNIKAGSNVLC 659
+ + +T+ R P+GF+T + N +A + +LC
Sbjct: 583 LVFSSWTLNLHKPSRTTIYVTLPLGFSTPLDKCNARAATPLLC 455
>AW171629 weakly similar to GP|14718995|gb| ceramide glucosyltransferase
{Gossypium arboreum}, partial (10%)
Length = 491
Score = 32.0 bits (71), Expect = 1.9
Identities = 21/87 (24%), Positives = 46/87 (52%), Gaps = 14/87 (16%)
Frame = +2
Query: 1053 LVQNNLNLEVIYGDTDSIMIYSGLDDIAKAT--------------SISKKVIQEVNKKYR 1098
L+ +NL++ +++G ++ S L ++ +A + K + EV+
Sbjct: 5 LIFHNLSIGLVFGTI*NMEKLSRLKELKEAKI*VQFSQIWEGNIYTERKGCLPEVHSLVL 184
Query: 1099 CLEIDLDGLYKRMLLLKKKKYAAVKVQ 1125
CLE+ L+G+ ++ L+ ++K AAVK++
Sbjct: 185 CLEVLLNGINRKNLITRRKLQAAVKLR 265
>TC92154
Length = 332
Score = 32.0 bits (71), Expect = 1.9
Identities = 21/58 (36%), Positives = 31/58 (53%)
Frame = -1
Query: 5 DGSSRKRRPTRGPDATARSQALELLRSRRSNGPRSTTTPQIRLENPIYDTIPEDEYTA 62
D + ++PTR D T ++ + ++RS G +TTT IR P Y+ DEYTA
Sbjct: 302 DHDVKIQQPTRLFDQTRQT----MCSAKRSLGHDTTTTRTIRSPTPSYELEVGDEYTA 141
>TC78189 homologue to GP|14485513|emb|CAC42087. putative NAC domain protein
{Solanum tuberosum}, partial (56%)
Length = 1556
Score = 31.6 bits (70), Expect = 2.4
Identities = 16/46 (34%), Positives = 23/46 (49%)
Frame = +2
Query: 862 PDKFSNYAKETKLTKRRVTHGVDDGNFDDADINDANYHNDASESDH 907
P FS + ETK + +G DD + D + +H D+S SDH
Sbjct: 614 PPMFSQFENETKPHIKMNMNGQDDQLYTDTSDSVPLFHTDSSGSDH 751
>TC78638 similar to GP|22087278|gb|AAM90909.1 p44/SSL1-like protein
{Arabidopsis thaliana}, partial (85%)
Length = 2060
Score = 31.6 bits (70), Expect = 2.4
Identities = 26/86 (30%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
Frame = +2
Query: 1031 YAKPLAELITLQGREILQSTVDLVQNNLNLEVIYGDTDSIMIYSGLDDIAKATSISKKVI 1090
+ K L + G LQ+ ++LV +NLN YG + +++YS L + + I
Sbjct: 803 HIKALMGKLECSGDASLQNALELVHSNLNQIPSYGHREVLILYSALSTCDPGDLM--ETI 976
Query: 1091 QEVNK-KYRCLEIDLDGLYKRMLLLK 1115
Q+ K K RC I GL M + K
Sbjct: 977 QKCKKSKIRCSVI---GLAAEMFICK 1045
>TC77900 similar to GP|9759182|dbj|BAB09797.1 ATP-dependent Clp protease
regulatory subunit CLPX {Arabidopsis thaliana}, partial
(61%)
Length = 2144
Score = 30.4 bits (67), Expect = 5.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 319 EVVCYGWMAGG*EWWRWRWGGKCC* 343
E+ C+ W WWRWRW +CC*
Sbjct: 557 EL*CFWW------WWRWRW-WRCC* 610
>TC82248 similar to GP|15216028|emb|CAC51424. amino acid permease AAP3 {Vicia
faba var. minor}, partial (29%)
Length = 779
Score = 29.6 bits (65), Expect = 9.2
Identities = 16/45 (35%), Positives = 24/45 (52%), Gaps = 1/45 (2%)
Frame = -3
Query: 1407 TTRSVSFRLVGDSERGTVCP-NYPRCNGHLNRKYTEADLYKQLSY 1450
TT + RLV + + VC N+ RCN H N Y + +Y ++ Y
Sbjct: 201 TTSPLGSRLVESAVQLGVCK*NHCRCNSHFNTIYIHSYIYIKILY 67
>TC82271 similar to GP|18491137|gb|AAL69537.1 AT5g45420/MFC19_9 {Arabidopsis
thaliana}, partial (43%)
Length = 1116
Score = 26.2 bits (56), Expect(2) = 9.5
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +2
Query: 120 CFTDQASFCFFFCG*IISCCCYDGRIKSFVDVYFC*F*EK 159
CF + C + G SCCC G IKS + C* E+
Sbjct: 773 CFESLSERCCYEMG--ESCCCCSGEIKSCLYEKNC*IEER 886
Score = 21.6 bits (44), Expect(2) = 9.5
Identities = 13/32 (40%), Positives = 17/32 (52%), Gaps = 3/32 (9%)
Frame = +1
Query: 79 RRIWL*R*R---RRRGLVQNRFFPFLRRRIRW 107
RR+W R RRR +++ R F RR RW
Sbjct: 586 RRVW*RNQRSWERRRLMIRIRMSSF*RREKRW 681
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.337 0.147 0.483
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,280,633
Number of Sequences: 36976
Number of extensions: 715031
Number of successful extensions: 6343
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 3574
Number of HSP's successfully gapped in prelim test: 255
Number of HSP's that attempted gapping in prelim test: 2543
Number of HSP's gapped (non-prelim): 4175
length of query: 1510
length of database: 9,014,727
effective HSP length: 109
effective length of query: 1401
effective length of database: 4,984,343
effective search space: 6983064543
effective search space used: 6983064543
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC144431.4