BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144430.8 - phase: 0 /pseudo
(91 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG585866 54 1e-08
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 43 2e-05
BG586862 41 9e-05
TC93101 37 0.002
BG585499 35 0.005
BG452711 32 0.042
BE124667 31 0.093
BG453645 30 0.12
TC82287 similar to GP|9294226|dbj|BAB02128.1 DNA-binding protein... 30 0.21
TC80955 similar to GP|17221601|dbj|BAB78467. GPRI2 {Arabidopsis ... 30 0.21
TC80651 similar to GP|15341529|gb|AAK95654.1 ferric-chelate redu... 27 1.0
AW560447 similar to PIR|T14520|T14 probable S-receptor kinase (E... 27 1.3
AA660473 27 1.3
AW690000 27 1.8
AW694627 similar to PIR|F86442|F864 unknown protein [imported] -... 26 2.3
TC76477 similar to GP|7320865|emb|CAB81951.1 integral membrane t... 26 2.3
AI737486 similar to GP|6094552|gb unknown protein {Arabidopsis t... 26 2.3
TC76441 similar to GP|14190501|gb|AAK55731.1 AT4g30010/F6G3_40 {... 26 2.3
BQ136093 26 2.3
TC93029 23 2.8
>BG585866
Length = 828
Score = 53.5 bits (127), Expect = 1e-08
Identities = 23/70 (32%), Positives = 37/70 (52%)
Frame = +3
Query: 1 MLHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFFLEHNLGWWIIIG 60
+L+ R M + +C+RC E FHC+ DC F + +W I F + +FF ++ W+ G
Sbjct: 540 LLNHRNMVNSAICSRCGEHEESFFHCVRDCRFSKIIWHKIGFSSPDFFSSSSVQDWLKDG 719
Query: 61 LDRQLPHLFL 70
+ P FL
Sbjct: 720 ISCHRPTTFL 749
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 42.7 bits (99), Expect = 2e-05
Identities = 27/94 (28%), Positives = 46/94 (48%), Gaps = 7/94 (7%)
Frame = +3
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW----LAISFDNI---NFFLEHNLG 54
L KRG+ +C RC+S +E + H + C + +W L I+FDN+ NF N
Sbjct: 210 LRKRGIQCYPLCPRCHSKTETITHLFMSCPLSKRVWFGSNLCINFDNLPNPNFI---N*L 380
Query: 55 WWIIIGLDRQLPHLFLSAVWLLWMARNRACIAQE 88
+ I+ D + + ++ LW ARN + + +
Sbjct: 381 YEAIL*KDECITI*IAAIIYNLWHARNLSVLEDQ 482
>BG586862
Length = 804
Score = 40.8 bits (94), Expect = 9e-05
Identities = 25/85 (29%), Positives = 40/85 (46%), Gaps = 4/85 (4%)
Frame = -1
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW----LAISFDNINFFLEHNLGWWI 57
LHKRG+ + +C RC S E V H L+C + W L I+F + H+
Sbjct: 573 LHKRGIRCSLLCPRCESKIETVQHLFLNCEVTQKEWFGSQLGINFHSSGVLHFHDWITNF 394
Query: 58 IIGLDRQLPHLFLSAVWLLWMARNR 82
I+ D + + ++ +W ARN+
Sbjct: 393 ILKNDEETIIALTALLYSIWHARNQ 319
>TC93101
Length = 675
Score = 36.6 bits (83), Expect = 0.002
Identities = 24/90 (26%), Positives = 40/90 (43%), Gaps = 8/90 (8%)
Frame = -1
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW----LAISF-DNINFFLEHNLGWW 56
L+KRG++ +C RC E H + C + +W L+I F DN N W
Sbjct: 645 LNKRGVNCPPLCPRCYFNLETTNHIFMSCERTQRVWFGSQLSIRFPDNSTI----NFSDW 478
Query: 57 IIIGLDRQLPHLFL---SAVWLLWMARNRA 83
+ + Q + + + + +W ARN+A
Sbjct: 477 LFDAISNQTEEIIIKISAITYSIWHARNKA 388
>BG585499
Length = 792
Score = 35.0 bits (79), Expect = 0.005
Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 10/81 (12%)
Frame = +3
Query: 13 CTRCNSTSEDVFHCLLDCAFPRNLWLAISFDN--INFFLEHNLGWWIIIGLDRQLPHL-- 68
C C + E V H L DC +W+ + + NFF + W+ L ++ +
Sbjct: 348 CPCCGNADETVLHVLCDCRPASQVWIRLVPSDWITNFFSFDDCRDWVFKNLSKRSNGVSK 527
Query: 69 ------FLSAVWLLWMARNRA 83
F++ W +W RN+A
Sbjct: 528 FKWQPTFMTTCWHMWTWRNKA 590
>BG452711
Length = 672
Score = 32.0 bits (71), Expect = 0.042
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +3
Query: 10 TDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDNINFFLEHNLGWWI 57
+++C CN S+D+ H L C +++W + + + NL W+
Sbjct: 399 SNLCPICNQRSQDMLHALFSCTRAKDVWSFCLHELVTSSNQDNLITWM 542
>BE124667
Length = 540
Score = 30.8 bits (68), Expect = 0.093
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = -1
Query: 12 VCTRCNSTSEDVFHCLLDCAFPRNLW 37
+C+ C ++SE FH +C F +W
Sbjct: 387 MCSSCQASSETTFHLFFECNFATKMW 310
>BG453645
Length = 622
Score = 30.4 bits (67), Expect = 0.12
Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 9/81 (11%)
Frame = +3
Query: 10 TDVCTRCNSTSEDVFHCLLDCAFPRNLWLAI-SFDNINFFLEHNL--GW--WIIIGLDRQ 64
+ C CN E H L C +W + + ++NF + NL W W G +
Sbjct: 24 SSTCVLCNLKVETTTHIFLHCDVASLVWFRVMKWFDVNFLIPPNLFVHWECWSEGGSANR 203
Query: 65 LPH----LFLSAVWLLWMARN 81
+ ++ + +W+LW RN
Sbjct: 204 VTKGHWLVWHTTIWVLWAKRN 266
>TC82287 similar to GP|9294226|dbj|BAB02128.1 DNA-binding protein-like
{Arabidopsis thaliana}, partial (96%)
Length = 795
Score = 29.6 bits (65), Expect = 0.21
Identities = 16/53 (30%), Positives = 26/53 (48%), Gaps = 5/53 (9%)
Frame = +1
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLW-----LAISFDNINFFL 49
LH PT + ++CN + HCL +C +LW L +S ++FF+
Sbjct: 16 LHFN*QHPTPLLSQCNCLLQI*HHCLSNCTLFLHLWSLSLPLYLSHHFLHFFI 174
>TC80955 similar to GP|17221601|dbj|BAB78467. GPRI2 {Arabidopsis thaliana},
partial (22%)
Length = 817
Score = 29.6 bits (65), Expect = 0.21
Identities = 13/48 (27%), Positives = 18/48 (37%)
Frame = -2
Query: 37 WLAISFDNINFFLEHNLGWWIIIGLDRQLPHLFLSAVWLLWMARNRAC 84
WL +N +L H +GWW W W A++R C
Sbjct: 441 WLTGGDNNTGVYLVHGMGWW---------------NAWNSWRAKSRCC 343
>TC80651 similar to GP|15341529|gb|AAK95654.1 ferric-chelate reductase
{Pisum sativum}, partial (32%)
Length = 984
Score = 27.3 bits (59), Expect = 1.0
Identities = 13/43 (30%), Positives = 22/43 (50%)
Frame = -3
Query: 2 LHKRGMSPTDVCTRCNSTSEDVFHCLLDCAFPRNLWLAISFDN 44
+ K +S + R TS FH ++ C FP + + +SFD+
Sbjct: 586 MSKNAISVIETIPRGPFTSIGRFHMVVPCFFPLRISMLLSFDD 458
>AW560447 similar to PIR|T14520|T14 probable S-receptor kinase (EC 2.7.1.-)
SFR3 precursor - wild cabbage, partial (12%)
Length = 641
Score = 26.9 bits (58), Expect = 1.3
Identities = 16/47 (34%), Positives = 19/47 (40%)
Frame = +1
Query: 36 LWLAISFDNINFFLEHNLGWWIIIGLDRQLPHLFLSAVWLLWMARNR 82
LW F + LGW + GL+RQL W WM R R
Sbjct: 421 LWQGFDFPCDTLLPDMKLGWDLKTGLNRQL------TSWKNWMIRLR 543
>AA660473
Length = 655
Score = 26.9 bits (58), Expect = 1.3
Identities = 11/30 (36%), Positives = 16/30 (52%)
Frame = -1
Query: 6 GMSPTDVCTRCNSTSEDVFHCLLDCAFPRN 35
G+ T C CN+T+ VF +C F R+
Sbjct: 577 GVQQTQGCQTCNTTTSGVFEGSTNCDFGRH 488
>AW690000
Length = 652
Score = 26.6 bits (57), Expect = 1.8
Identities = 8/26 (30%), Positives = 14/26 (53%)
Frame = +2
Query: 12 VCTRCNSTSEDVFHCLLDCAFPRNLW 37
+C+ C +E H +C++ NLW
Sbjct: 11 MCSLCCKQAESSLHLFFECSYAVNLW 88
>AW694627 similar to PIR|F86442|F864 unknown protein [imported] - Arabidopsis
thaliana, partial (5%)
Length = 492
Score = 26.2 bits (56), Expect = 2.3
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 3/40 (7%)
Frame = -1
Query: 8 SPTDVCTRCNSTSEDVFHCLLD---CAFPRNLWLAISFDN 44
SP D C CN S+ HC+ + F +NL + IS+ N
Sbjct: 429 SPLDQCMECNLDSQ---HCIQEN*WI*FAKNLLMMISWGN 319
>TC76477 similar to GP|7320865|emb|CAB81951.1 integral membrane transporter
protein {Homo sapiens}, partial (12%)
Length = 1164
Score = 26.2 bits (56), Expect = 2.3
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -3
Query: 14 TRCNSTSEDVFHCLLDCAFPRNL 36
+RC +S + +C +D A PRNL
Sbjct: 628 SRCRISSSPLLNCSIDSANPRNL 560
>AI737486 similar to GP|6094552|gb unknown protein {Arabidopsis thaliana},
partial (9%)
Length = 473
Score = 26.2 bits (56), Expect = 2.3
Identities = 11/28 (39%), Positives = 14/28 (49%)
Frame = -3
Query: 6 GMSPTDVCTRCNSTSEDVFHCLLDCAFP 33
G S +VC+R E +F C C FP
Sbjct: 390 GSSENNVCSR*GQVLEWIFPCTSPCIFP 307
>TC76441 similar to GP|14190501|gb|AAK55731.1 AT4g30010/F6G3_40 {Arabidopsis
thaliana}, partial (46%)
Length = 444
Score = 26.2 bits (56), Expect = 2.3
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -2
Query: 14 TRCNSTSEDVFHCLLDCAFPRNL 36
+RC +S + +C +D A PRNL
Sbjct: 152 SRCRISSSPLLNCSIDSANPRNL 84
>BQ136093
Length = 838
Score = 26.2 bits (56), Expect = 2.3
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -1
Query: 14 TRCNSTSEDVFHCLLDCAFPRNL 36
+RC +S + +C +D A PRNL
Sbjct: 88 SRCRISSSPLLNCSIDSANPRNL 20
>TC93029
Length = 821
Score = 23.5 bits (49), Expect(2) = 2.8
Identities = 11/27 (40%), Positives = 13/27 (47%)
Frame = -3
Query: 4 KRGMSPTDVCTRCNSTSEDVFHCLLDC 30
K ++P T N TS D FH L C
Sbjct: 567 KATLNPFSHLTPTNQTSFDSFHLTLSC 487
Score = 20.8 bits (42), Expect(2) = 2.8
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = -3
Query: 26 CLLDCAFPRNLWLAISFDNINFF 48
C+ A LAISFD +N F
Sbjct: 408 CITRSANTNTTKLAISFDGLNKF 340
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.331 0.141 0.509
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,841,096
Number of Sequences: 36976
Number of extensions: 85990
Number of successful extensions: 634
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 634
length of query: 91
length of database: 9,014,727
effective HSP length: 67
effective length of query: 24
effective length of database: 6,537,335
effective search space: 156896040
effective search space used: 156896040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC144430.8