
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144430.5 - phase: 0 /pseudo
(125 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89625 weakly similar to GP|21594393|gb|AAM66003.1 unknown {Ara... 216 1e-57
BF649540 similar to GP|12597822|gb| hypothetical protein {Arabid... 28 0.93
TC91486 similar to GP|5441893|dbj|BAA82391.1 ESTs C99174(E10437)... 27 1.6
TC93429 weakly similar to PIR|B96660|B96660 protein F2K11.18 [im... 27 2.1
BI309938 weakly similar to GP|19920182|gb Putative Glucan 1 3-be... 27 2.7
BQ751207 weakly similar to GP|604427|gb|AA ACOB protein {Emerice... 26 3.5
TC86329 similar to PIR|T07171|T07171 subtilisin-like proteinase ... 26 4.6
TC79924 weakly similar to GP|15146278|gb|AAK83622.1 AT5g20070/F2... 26 4.6
BI265510 weakly similar to PIR|C84555|C845 hypothetical protein ... 26 4.6
BG450115 25 6.0
>TC89625 weakly similar to GP|21594393|gb|AAM66003.1 unknown {Arabidopsis
thaliana}, partial (74%)
Length = 721
Score = 216 bits (551), Expect = 1e-57
Identities = 112/112 (100%), Positives = 112/112 (100%)
Frame = +2
Query: 14 AANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKL 73
AANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKL
Sbjct: 20 AANRRRPLWRPMSTQNAATGGDNSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKL 199
Query: 74 PKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNVPQLKTVVQLLANM 125
PKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNVPQLKTVVQLLANM
Sbjct: 200 PKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNVPQLKTVVQLLANM 355
>BF649540 similar to GP|12597822|gb| hypothetical protein {Arabidopsis
thaliana}, partial (13%)
Length = 656
Score = 28.1 bits (61), Expect = 0.93
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +1
Query: 58 HLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQN 111
H SSP+ K L K LL ++ ++ + +++Q ++S +AH +NG N
Sbjct: 310 HREYRSSPSTKKQ-SLKKRLLISLNELEKLKPLVQQKINELNSRNAHQQNGRGN 468
>TC91486 similar to GP|5441893|dbj|BAA82391.1 ESTs C99174(E10437)
D22295(C10709) correspond to a region of the predicted
gene.~Similar to, partial (25%)
Length = 736
Score = 27.3 bits (59), Expect = 1.6
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +3
Query: 55 NLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENG 108
NLLHL++ P + L + N+L K + V T +L + +++S M NG
Sbjct: 405 NLLHLLEMLPPHRQSLTMVLHNMLLKAAYV--TSLILLKAANLVTSAILPMVNG 560
>TC93429 weakly similar to PIR|B96660|B96660 protein F2K11.18 [imported] -
Arabidopsis thaliana, partial (16%)
Length = 1080
Score = 26.9 bits (58), Expect = 2.1
Identities = 16/61 (26%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = -2
Query: 1 MKKMEQRRQDPPTAANRRRPLWRPMSTQNAATG--GDNSAKAVVAEQISQTVQSTSNLLH 58
+ ++ Q RQ R++ LW P+S + T D S+ Q++ + + S +LH
Sbjct: 281 LSQLIQHRQHMSFP*RRKQQLWPPLSLHPSTTTFLSDTSSSTDSVTQVTGSHSTASAILH 102
Query: 59 L 59
L
Sbjct: 101 L 99
>BI309938 weakly similar to GP|19920182|gb Putative Glucan 1
3-beta-glucosidase precursor {Oryza sativa (japonica
cultivar-group)}, partial (29%)
Length = 802
Score = 26.6 bits (57), Expect = 2.7
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = +3
Query: 34 GDNSAKAVVA--EQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQQVL 91
GD+S VA + ++Q + ++L+ + + P L L K A V+NT+QVL
Sbjct: 489 GDSSISDTVATIDFLAQRYANRTSLIAIQLMNEPQGVDLGSLKKYYQAGYDAVRNTRQVL 668
>BQ751207 weakly similar to GP|604427|gb|AA ACOB protein {Emericella
nidulans}, partial (12%)
Length = 758
Score = 26.2 bits (56), Expect = 3.5
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +1
Query: 11 PPTAANRRRPLWRPMSTQNAA 31
PPT++ R P PMS+QN++
Sbjct: 112 PPTSSPARHPTQTPMSSQNSS 174
>TC86329 similar to PIR|T07171|T07171 subtilisin-like proteinase (EC
3.4.21.-) 1 - tomato, partial (91%)
Length = 2671
Score = 25.8 bits (55), Expect = 4.6
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +3
Query: 75 KNLLAKVSTVKNTQQVLEQLPRVISSLDAHMENGLQNVPQLKTVV 119
+NLL + +K T+Q+L+ L + + M G++ PQL+ V
Sbjct: 618 RNLLVQGFLLKATRQLLDLLMKTRNQSHQGMMTGMEVTPQLQQQV 752
>TC79924 weakly similar to GP|15146278|gb|AAK83622.1 AT5g20070/F28I16_220
{Arabidopsis thaliana}, partial (21%)
Length = 628
Score = 25.8 bits (55), Expect = 4.6
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = -2
Query: 48 QTVQSTSNLLHLMQHSSP 65
QT QS SN LHL+QHS+P
Sbjct: 453 QTHQS-SNKLHLLQHSNP 403
>BI265510 weakly similar to PIR|C84555|C845 hypothetical protein At2g17690
[imported] - Arabidopsis thaliana, partial (8%)
Length = 545
Score = 25.8 bits (55), Expect = 4.6
Identities = 17/53 (32%), Positives = 30/53 (56%)
Frame = +2
Query: 36 NSAKAVVAEQISQTVQSTSNLLHLMQHSSPAQAKLVKLPKNLLAKVSTVKNTQ 88
+S+ ++ Q + STS+ +L SS A+A +LPK+LL +S +T+
Sbjct: 29 SSSLSLSLRQHHLRLSSTSSSSNLGASSSMAEADWSQLPKDLLTLISQRIDTE 187
>BG450115
Length = 664
Score = 25.4 bits (54), Expect = 6.0
Identities = 14/37 (37%), Positives = 19/37 (50%), Gaps = 1/37 (2%)
Frame = +2
Query: 34 GDNSAKAVVAEQISQTVQSTSNLLHL-MQHSSPAQAK 69
G AKA + EQ+S+ V T LH+ +H AK
Sbjct: 152 GAGHAKAGLGEQVSKNVAETKEKLHIGNKHGEATAAK 262
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.122 0.327
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,770,587
Number of Sequences: 36976
Number of extensions: 25588
Number of successful extensions: 154
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of query: 125
length of database: 9,014,727
effective HSP length: 84
effective length of query: 41
effective length of database: 5,908,743
effective search space: 242258463
effective search space used: 242258463
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144430.5