Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144406.5 - phase: 0 
         (47 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC76545 similar to GP|6119844|gb|AAF04253.1| short-chain alcohol...    30  0.26
BG586288 similar to GP|20258963|gb putative eukaryotic initiatio...    27  1.3
TC79820 similar to GP|21537325|gb|AAM61666.1 unknown {Arabidopsi...    26  3.7
CB065295 homologue to GP|3253202|gb| PhaF {Pseudomonas putida}, ...    25  4.9
TC84257 weakly similar to PIR|G83180|G83180 probable FMN oxidore...    25  6.4
TC87047 similar to GP|9989062|gb|AAG10825.1| Unknown protein {Ar...    25  8.3

>TC76545 similar to GP|6119844|gb|AAF04253.1| short-chain alcohol
          dehydrogenase SAD-C {Pisum sativum}, partial (95%)
          Length = 980

 Score = 29.6 bits (65), Expect = 0.26
 Identities = 11/18 (61%), Positives = 12/18 (66%)
 Frame = +3

Query: 20 HWKRPGTRACRERSTPGG 37
          HW+R GT  CR R T GG
Sbjct: 69 HWQRDGTSLCRTRCTHGG 122


>BG586288 similar to GP|20258963|gb putative eukaryotic initiation factor 4
           {Arabidopsis thaliana}, partial (20%)
          Length = 710

 Score = 27.3 bits (59), Expect = 1.3
 Identities = 16/47 (34%), Positives = 22/47 (46%)
 Frame = +3

Query: 1   MGGRSRCPGRTRLLLTLEDHWKRPGTRACRERSTPGGRASTVSPGHS 47
           M G  R PG     +   D+W+ P TR+   R  PGG   + + G S
Sbjct: 294 MPGTRRMPGAP---VGDNDNWEVPRTRSMSRRDGPGGSLHSPAMGKS 425


>TC79820 similar to GP|21537325|gb|AAM61666.1 unknown {Arabidopsis
           thaliana}, partial (89%)
          Length = 1225

 Score = 25.8 bits (55), Expect = 3.7
 Identities = 12/24 (50%), Positives = 13/24 (54%)
 Frame = +2

Query: 4   RSRCPGRTRLLLTLEDHWKRPGTR 27
           RSRC  R  LLL    H  RP +R
Sbjct: 212 RSRCSPRCSLLLPFRSHLPRPHSR 283


>CB065295 homologue to GP|3253202|gb| PhaF {Pseudomonas putida}, partial
           (46%)
          Length = 645

 Score = 25.4 bits (54), Expect = 4.9
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = -1

Query: 23  RPGTRACRERSTPGGRASTVSPGHS 47
           R   RA R  S PG R S ++PG++
Sbjct: 309 RVTARATRSASPPGARPSRMAPGYA 235


>TC84257 weakly similar to PIR|G83180|G83180 probable FMN oxidoreductase
           PA3723 [imported] - Pseudomonas aeruginosa (strain
           PAO1), partial (31%)
          Length = 777

 Score = 25.0 bits (53), Expect = 6.4
 Identities = 13/27 (48%), Positives = 15/27 (55%), Gaps = 1/27 (3%)
 Frame = -3

Query: 21  WKRPGTRACRE-RSTPGGRASTVSPGH 46
           W RP T   R  R+ PGGR  + S GH
Sbjct: 394 WPRPWTGRRRAGRTGPGGRGGSRSRGH 314


>TC87047 similar to GP|9989062|gb|AAG10825.1| Unknown protein {Arabidopsis
           thaliana}, partial (79%)
          Length = 1416

 Score = 24.6 bits (52), Expect = 8.3
 Identities = 10/21 (47%), Positives = 12/21 (56%)
 Frame = +3

Query: 4   RSRCPGRTRLLLTLEDHWKRP 24
           R RC G TRLL+  +  W  P
Sbjct: 720 RRRCNGETRLLICSDSPWILP 782


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.318    0.135    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,761,487
Number of Sequences: 36976
Number of extensions: 17415
Number of successful extensions: 120
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of query: 47
length of database: 9,014,727
effective HSP length: 23
effective length of query: 24
effective length of database: 8,164,279
effective search space: 195942696
effective search space used: 195942696
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)


Medicago: description of AC144406.5