BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144406.4 - phase: 1 /pseudo
(322 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ752218 similar to GP|19170914|emb hypothetical protein {Enceph... 31 0.73
BQ135482 weakly similar to GP|6062754|gb|A NADH dehydrogenase su... 30 1.6
TC87282 similar to GP|21553648|gb|AAM62741.1 Ser Thr specific pr... 29 2.1
BF632924 29 2.8
TC91437 similar to PIR|D86166|D86166 protein F21B7.12 [imported]... 29 2.8
BQ144235 similar to GP|6692770|dbj| lacZ alpha {Cloning vector p... 28 4.8
BQ141697 similar to GP|18159237|gb| conserved within P. aerophil... 28 4.8
AW585084 28 6.2
TC81272 similar to PIR|T05225|T05225 extensin homolog F17I5.160 ... 28 6.2
AJ501145 28 6.2
AW585567 27 8.1
>BQ752218 similar to GP|19170914|emb hypothetical protein {Encephalitozoon
cuniculi}, partial (5%)
Length = 637
Score = 30.8 bits (68), Expect = 0.73
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -3
Query: 77 RRPRRLKRRDKSRPGKEKGR 96
RRPRR +RR K RP K +GR
Sbjct: 518 RRPRRRRRRPKRRPRKRRGR 459
>BQ135482 weakly similar to GP|6062754|gb|A NADH dehydrogenase subunit II
{Cynolebias bokermanni}, partial (8%)
Length = 1061
Score = 29.6 bits (65), Expect = 1.6
Identities = 30/103 (29%), Positives = 45/103 (43%), Gaps = 9/103 (8%)
Frame = -1
Query: 135 RLDDREKQNEI-RIWPKKKQRYGYHDRSPSIKKNLSKSLRVSG-----AHPKYAGAVNDI 188
+L R K++E ++PKK++RY R + K+NL + G PK
Sbjct: 788 KLGGRRKRDEG*NLFPKKRKRYKLGVRRKNPKENLEERPLPVGPEKFSLEPKPPRG---- 621
Query: 189 AFLIENYDSFRKTKFFKLFFPRSDG---PTGHLFKRTLPAVRP 228
E ++ KFF PR+ G P G L ++LP RP
Sbjct: 620 ----ERRENIPNLKFFLKERPRAPGPQPPAGELRGKSLPPKRP 504
>TC87282 similar to GP|21553648|gb|AAM62741.1 Ser Thr specific protein
kinase-like protein {Arabidopsis thaliana}, partial
(27%)
Length = 1338
Score = 29.3 bits (64), Expect = 2.1
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = +3
Query: 41 YFGSIRPPARLTFGFRLGRCILIHFPKRTFI 71
+F PP L F C+L+HFP T+I
Sbjct: 225 FFFLAPPPHLLLLRFSCASCVLLHFPPTTYI 317
>BF632924
Length = 469
Score = 28.9 bits (63), Expect = 2.8
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 83 KRRDKSRPGKEKGRWWAFGKVGPIG 107
KR + R + + RWW GK+G G
Sbjct: 384 KRERQGREERRRNRWWRIGKIGDKG 458
>TC91437 similar to PIR|D86166|D86166 protein F21B7.12 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 843
Score = 28.9 bits (63), Expect = 2.8
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = +3
Query: 205 KLFFPRSDGPTGHLFKRTLPAVRPSLNYSVMQYF 238
++FF PT H + TLP ++ SL+ +++ +F
Sbjct: 117 RIFFYHFPHPTHHFLQTTLPILKHSLSITLLHFF 218
>BQ144235 similar to GP|6692770|dbj| lacZ alpha {Cloning vector pMG103},
partial (20%)
Length = 476
Score = 28.1 bits (61), Expect = 4.8
Identities = 14/52 (26%), Positives = 26/52 (49%)
Frame = -2
Query: 91 GKEKGRWWAFGKVGPIGCLHSSNKKDEERNEVRGRRAGKRVESIRLDDREKQ 142
G E G WW G+VG G + + +N +GR G+ + + +R+++
Sbjct: 379 GMEGGIWWKKGQVG*YGTHGIGEEGGKGKNWKKGRVGGEMKKGKKTGNRKRR 224
>BQ141697 similar to GP|18159237|gb| conserved within P. aerophilum
{Pyrobaculum aerophilum}, partial (4%)
Length = 1368
Score = 28.1 bits (61), Expect = 4.8
Identities = 14/43 (32%), Positives = 20/43 (45%)
Frame = -2
Query: 56 RLGRCILIHFPKRTFIHFFLPRRPRRLKRRDKSRPGKEKGRWW 98
R+ RC + + IH F R R +RRD++R G W
Sbjct: 965 RVSRCAI------SLIHLFASSRTARYRRRDRNRVASYNGLSW 855
>AW585084
Length = 379
Score = 27.7 bits (60), Expect = 6.2
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = -1
Query: 85 RDKSRPGKEKGRWWAFGKVGPIG 107
+D++ + +GRW+ G VGP+G
Sbjct: 82 QDRTFQDRGRGRWFVVGSVGPLG 14
>TC81272 similar to PIR|T05225|T05225 extensin homolog F17I5.160 -
Arabidopsis thaliana, partial (3%)
Length = 757
Score = 27.7 bits (60), Expect = 6.2
Identities = 22/78 (28%), Positives = 34/78 (43%), Gaps = 5/78 (6%)
Frame = -2
Query: 115 KDEERNEVRGRRAGKRVESIRLDDREKQN---EIRIWPKKK--QRYGYHDRSPSIKKNLS 169
+DEER RGR R R + R K E R W + + +R + DR+ +K
Sbjct: 426 RDEERRWPRGRAGEHRKHQGRAEGRRKHQGRAEGRHWHQGRAGERQRHRDRAGEHRKRQD 247
Query: 170 KSLRVSGAHPKYAGAVND 187
+ +G H K+ G +
Sbjct: 246 R----AGEHRKHQGRAEE 205
>AJ501145
Length = 629
Score = 27.7 bits (60), Expect = 6.2
Identities = 15/58 (25%), Positives = 30/58 (50%)
Frame = +2
Query: 111 SSNKKDEERNEVRGRRAGKRVESIRLDDREKQNEIRIWPKKKQRYGYHDRSPSIKKNL 168
S+ ++ ++ +GR G + R+ +E Q+ P+KK Y +HDR S++ +
Sbjct: 392 STRRRTSKKKRKKGRNTGPK----RVFGQETQSNA---PRKKYYYFHHDRGVSVQSGV 544
>AW585567
Length = 491
Score = 27.3 bits (59), Expect = 8.1
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 114 KKDEERNEVRGRRAGKRVESIRLDDREKQNEIRIWPK 150
KK + N R RR + S+ + KQN+I+++PK
Sbjct: 350 KKKQNLNR*RRRRNNTDLFSVSKQEINKQNQIQVFPK 460
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.140 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,989,429
Number of Sequences: 36976
Number of extensions: 164136
Number of successful extensions: 901
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 894
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 901
length of query: 322
length of database: 9,014,727
effective HSP length: 96
effective length of query: 226
effective length of database: 5,465,031
effective search space: 1235097006
effective search space used: 1235097006
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144406.4