BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144375.4 - phase: 0
(340 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86223 similar to GP|12597759|gb|AAG60072.1 pheromone receptor ... 681 0.0
BQ153826 weakly similar to GP|21689777|gb unknown protein {Arabi... 57 8e-09
TC86990 similar to GP|22136840|gb|AAM91764.1 unknown protein {Ar... 49 3e-06
TC82012 similar to PIR|T04782|T04782 hypothetical protein F10M10... 49 4e-06
TC92088 similar to GP|21689773|gb|AAM67530.1 unknown protein {Ar... 38 0.006
TC78111 similar to GP|17224292|gb|AAL36933.1 gamma-tocopherol me... 31 0.60
AJ547977 29 2.3
TC84334 weakly similar to PIR|T49308|T49308 hypothetical protein... 29 2.3
BG454624 weakly similar to SP|P34121|P17_D Cytoskeletal P17 prot... 28 3.9
BF006775 weakly similar to GP|16905203|gb| putative receptor-lik... 28 5.1
TC86570 homologue to GP|11094365|gb|AAG29593.1 Ser/Thr specific ... 28 5.1
TC89177 27 8.7
>TC86223 similar to GP|12597759|gb|AAG60072.1 pheromone receptor putative
(AR401) {Arabidopsis thaliana}, partial (79%)
Length = 1615
Score = 681 bits (1756), Expect = 0.0
Identities = 340/340 (100%), Positives = 340/340 (100%)
Frame = +3
Query: 1 MAGIRLQPEDSDVSQQARAVALVDLVSDDDRSIAADSWSIKSEYGSTLDDDQRHADAAEA 60
MAGIRLQPEDSDVSQQARAVALVDLVSDDDRSIAADSWSIKSEYGSTLDDDQRHADAAEA
Sbjct: 486 MAGIRLQPEDSDVSQQARAVALVDLVSDDDRSIAADSWSIKSEYGSTLDDDQRHADAAEA 665
Query: 61 LSNVNLRAASDYSSDKDEPDAEAVSSMLGFQSYWDAAYTDELTNFHEHGHAGEVWFGDNV 120
LSNVNLRAASDYSSDKDEPDAEAVSSMLGFQSYWDAAYTDELTNFHEHGHAGEVWFGDNV
Sbjct: 666 LSNVNLRAASDYSSDKDEPDAEAVSSMLGFQSYWDAAYTDELTNFHEHGHAGEVWFGDNV 845
Query: 121 MEVVASWTKTLCIDISQGRLPNHVDDVKADAGELDDKLLSSWNVLDIGTGNGLLLQELAK 180
MEVVASWTKTLCIDISQGRLPNHVDDVKADAGELDDKLLSSWNVLDIGTGNGLLLQELAK
Sbjct: 846 MEVVASWTKTLCIDISQGRLPNHVDDVKADAGELDDKLLSSWNVLDIGTGNGLLLQELAK 1025
Query: 181 QGFSDLTGTDYSERAINLAQSLANRDGFPNIKFLVDDVLETKLEQVFQLVMDKGTLDAIG 240
QGFSDLTGTDYSERAINLAQSLANRDGFPNIKFLVDDVLETKLEQVFQLVMDKGTLDAIG
Sbjct: 1026QGFSDLTGTDYSERAINLAQSLANRDGFPNIKFLVDDVLETKLEQVFQLVMDKGTLDAIG 1205
Query: 241 LHPDGPVKRMMYWDSVSRLVAPGGILVVTSCNSTKDELVQEVESFNQRKSATAPEPEVAK 300
LHPDGPVKRMMYWDSVSRLVAPGGILVVTSCNSTKDELVQEVESFNQRKSATAPEPEVAK
Sbjct: 1206LHPDGPVKRMMYWDSVSRLVAPGGILVVTSCNSTKDELVQEVESFNQRKSATAPEPEVAK 1385
Query: 301 DDESCRDPLFQYVSHVRTYPTFMFGGSVGSRVATVAFLRK 340
DDESCRDPLFQYVSHVRTYPTFMFGGSVGSRVATVAFLRK
Sbjct: 1386DDESCRDPLFQYVSHVRTYPTFMFGGSVGSRVATVAFLRK 1505
>BQ153826 weakly similar to GP|21689777|gb unknown protein {Arabidopsis
thaliana}, partial (73%)
Length = 569
Score = 57.4 bits (137), Expect = 8e-09
Identities = 32/106 (30%), Positives = 55/106 (51%), Gaps = 2/106 (1%)
Frame = +3
Query: 161 SWNVLDIGTGNGLLLQELAKQGFSDLTGTDYSERAINLAQSLANRDGFPNIKFLVDDVLE 220
S +L +G GN ++ +++ K G+ ++ D S AI++ + R P +K+L DV +
Sbjct: 60 SSTILMVGCGNAVISEDMVKDGYEEIVNIDISSVAIDMMRKKHER--LPQLKYLQMDVRD 233
Query: 221 TKL--EQVFQLVMDKGTLDAIGLHPDGPVKRMMYWDSVSRLVAPGG 264
++ F V+DKGTLD++ D P+ V L+ PGG
Sbjct: 234 MSFFPDESFDGVIDKGTLDSLMCGTDAPISASQMLAEVCXLLKPGG 371
>TC86990 similar to GP|22136840|gb|AAM91764.1 unknown protein {Arabidopsis
thaliana}, partial (64%)
Length = 2354
Score = 48.9 bits (115), Expect = 3e-06
Identities = 37/111 (33%), Positives = 57/111 (51%), Gaps = 3/111 (2%)
Frame = +1
Query: 160 SSWNVLDIGTGNGLLLQELAKQGFSDLTGTDYSERAIN--LAQSLANRDGFPNIKFLVDD 217
+S VL G GN L + L GF+ +T D+S+ I L +++ +R P +++ V D
Sbjct: 241 ASLPVLVPGCGNSRLSEHLYDAGFTSITNIDFSKVVIGDMLRRNIRSR---PLMRWRVMD 411
Query: 218 VLETKLE-QVFQLVMDKGTLDAIGLHPDGPVKRMMYWDSVSRLVAPGGILV 267
+ + E + F V+DKG LDA+ GP Y V R++ PGG V
Sbjct: 412 MTAMQFEDEFFGAVVDKGGLDALMEPELGPTLGNQYLSEVKRVLKPGGKFV 564
>TC82012 similar to PIR|T04782|T04782 hypothetical protein F10M10.130 -
Arabidopsis thaliana, partial (79%)
Length = 772
Score = 48.5 bits (114), Expect = 4e-06
Identities = 27/85 (31%), Positives = 47/85 (54%), Gaps = 1/85 (1%)
Frame = +1
Query: 163 NVLDIGTGNGLLLQELAKQGFSDLTGTDYSERAINLAQSLANRDGFPNIKFLVDDVLETK 222
+VL++G GN + L G +++T D S A++ Q+ GF +IK + D+LE
Sbjct: 151 SVLELGCGNSQMCDGLYNDGTTNITCIDLSHVAVHNMQNRLLSQGFKDIKVMQADMLELP 330
Query: 223 LE-QVFQLVMDKGTLDAIGLHPDGP 246
+ + F LV++KGT+D + + P
Sbjct: 331 FDDECFDLVIEKGTMDVLFVDSGDP 405
>TC92088 similar to GP|21689773|gb|AAM67530.1 unknown protein {Arabidopsis
thaliana}, partial (64%)
Length = 783
Score = 37.7 bits (86), Expect = 0.006
Identities = 27/80 (33%), Positives = 42/80 (51%), Gaps = 3/80 (3%)
Frame = +3
Query: 163 NVLDIGTGNGLLLQELAKQ-GFSDLTGTDYSERAINLAQSLANRDGFPNIKFLVDDVLET 221
++L +G+GN Q + + G+ D+ D S I+ Q RD P +K+L DV +
Sbjct: 291 SILVVGSGNSAFSQGMVDESGYKDVVNIDISSVVIDAMQK-KYRDR-PQLKYLKMDVRDM 464
Query: 222 KL--EQVFQLVMDKGTLDAI 239
+ F V+DKGTLD+I
Sbjct: 465 SAFASETFGSVIDKGTLDSI 524
>TC78111 similar to GP|17224292|gb|AAL36933.1 gamma-tocopherol
methyltransferase {Perilla frutescens}, partial (41%)
Length = 956
Score = 31.2 bits (69), Expect = 0.60
Identities = 32/116 (27%), Positives = 54/116 (45%), Gaps = 5/116 (4%)
Frame = +1
Query: 160 SSW--NVLDIGTGNGLLLQELAKQGFSDLTGTDYSERAINLAQSLANRDGFPN-IKFLVD 216
S W +V+D+G G G + LAK+ ++ G S A +LA G + + F V
Sbjct: 631 SKWPKSVVDVGCGIGGSSRYLAKKFGANCVGITLSPVQAERANALAAAQGLADKVSFQVA 810
Query: 217 DVLETKL-EQVFQLVMDKGTLDAIGLH-PDGPVKRMMYWDSVSRLVAPGGILVVTS 270
D L+ + F LV + G H P+ P + ++R+ APGG +++ +
Sbjct: 811 DALQQPFPDGQFDLVWSMES----GEHMPNKP----KFVGELARVAAPGGTIIIVT 954
>AJ547977
Length = 266
Score = 29.3 bits (64), Expect = 2.3
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 5/50 (10%)
Frame = -3
Query: 44 YGSTLDDDQRHADAAEALSNVNLRAASDYSSDKDE-----PDAEAVSSML 88
+ LDDD E L+N+NL + SDK++ P A++V +L
Sbjct: 204 FDGVLDDDPNEQTIGEKLTNLNLLDENKSKSDKEQVSSKPPSADSVDVLL 55
>TC84334 weakly similar to PIR|T49308|T49308 hypothetical protein T16L24.220
- Arabidopsis thaliana, partial (14%)
Length = 804
Score = 29.3 bits (64), Expect = 2.3
Identities = 20/85 (23%), Positives = 37/85 (43%), Gaps = 11/85 (12%)
Frame = +1
Query: 9 EDSDVSQQARAVALVDLVSDD-----------DRSIAADSWSIKSEYGSTLDDDQRHADA 57
E+ +S A V V+ V D+ D + SW+ K + GST ++D +
Sbjct: 52 EEKGLSDMANPVVKVESVLDEQIGTTSSKVFEDAEVDIVSWTNKGDVGSTKNEDPDATEY 231
Query: 58 AEALSNVNLRAASDYSSDKDEPDAE 82
+ + ++ + A + S E D+E
Sbjct: 232 SSSFADTDSDAENSSRSSDAEVDSE 306
>BG454624 weakly similar to SP|P34121|P17_D Cytoskeletal P17 protein
(Coactosin) (Cyclic AMP-regulated protein P16). [Slime
mold], partial (56%)
Length = 537
Score = 28.5 bits (62), Expect = 3.9
Identities = 15/57 (26%), Positives = 29/57 (50%)
Frame = +1
Query: 133 IDISQGRLPNHVDDVKADAGELDDKLLSSWNVLDIGTGNGLLLQELAKQGFSDLTGT 189
+D+S+ +L + DDV++D E ++W V N ++++ G +L GT
Sbjct: 49 VDVSEPQLKSLYDDVRSDGNE------TNWVVFGYEGNNKIVVKGSGAGGLDELKGT 201
>BF006775 weakly similar to GP|16905203|gb| putative receptor-like protein
kinase {Oryza sativa}, partial (6%)
Length = 452
Score = 28.1 bits (61), Expect = 5.1
Identities = 17/66 (25%), Positives = 35/66 (52%), Gaps = 2/66 (3%)
Frame = +1
Query: 120 VMEVVASWTKTLCIDISQGRLPNHVDDVKADAGELD--DKLLSSWNVLDIGTGNGLLLQE 177
V+ VVAS +CI + + + +++D++ D +++ + LL ++ L + T N +
Sbjct: 166 VVVVVASLLIFICICVRKRKAKINLEDIEDDNNDIEIAESLLFNFETLRVATSNFSEANK 345
Query: 178 LAKQGF 183
L GF
Sbjct: 346 LGHGGF 363
>TC86570 homologue to GP|11094365|gb|AAG29593.1 Ser/Thr specific protein
phosphatase 2A A regulatory subunit alpha isoform,
partial (35%)
Length = 883
Score = 28.1 bits (61), Expect = 5.1
Identities = 20/49 (40%), Positives = 27/49 (54%), Gaps = 3/49 (6%)
Frame = +2
Query: 203 ANRDGFPNIKFLVDDVLETKLEQVFQLVMDK---GTLDAIGLHPDGPVK 248
A++D PNIKF V VLE+ V Q V++K L +G PD V+
Sbjct: 434 ASKDRVPNIKFNVAKVLESIFPIVDQSVVEKTIRPCLVELGEDPDVDVR 580
>TC89177
Length = 1201
Score = 27.3 bits (59), Expect = 8.7
Identities = 18/71 (25%), Positives = 29/71 (40%)
Frame = +2
Query: 10 DSDVSQQARAVALVDLVSDDDRSIAADSWSIKSEYGSTLDDDQRHADAAEALSNVNLRAA 69
D DVS+ R + + + DDD D S + Y +++D D+ A
Sbjct: 350 DDDVSEDCRIILIANTGDDDDE----DYGSFLTTYNPGIENDSDKVDSDFATFLATYDPG 517
Query: 70 SDYSSDKDEPD 80
+ SDK + D
Sbjct: 518 VEIDSDKADLD 550
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,264,224
Number of Sequences: 36976
Number of extensions: 115055
Number of successful extensions: 354
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 353
length of query: 340
length of database: 9,014,727
effective HSP length: 97
effective length of query: 243
effective length of database: 5,428,055
effective search space: 1319017365
effective search space used: 1319017365
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144375.4