BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.5 - phase: 0
(131 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG581966 similar to GP|3776080|emb MtN9 {Medicago truncatula}, p... 176 2e-45
TC86917 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago trun... 160 1e-40
TC86916 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago trun... 82 2e-33
TC79334 MtN9 136 2e-33
TC90008 weakly similar to GP|4249376|gb|AAD14473.1| Strong simil... 96 5e-21
TC84571 weakly similar to SP|P29136|MEP1_SOYBN Metalloendoprotei... 47 2e-06
TC82218 similar to GP|7159629|emb|CAB76364.1 matrix metalloprote... 47 3e-06
TC81657 weakly similar to PIR|T51957|T51957 metalloproteinase (E... 45 6e-06
TC79163 weakly similar to PIR|E96724|E96724 hypothetical protein... 32 0.072
TC87538 weakly similar to GP|21593148|gb|AAM65097.1 unknown {Ara... 29 0.47
CA917134 weakly similar to PIR|E96724|E96 hypothetical protein F... 29 0.61
BQ148602 similar to GP|6729043|gb|A hypothetical protein {Arabid... 27 1.8
TC88061 homologue to SP|Q43117|KPYA_RICCO Pyruvate kinase isozym... 27 3.0
TC92804 26 5.2
BG645531 26 5.2
TC90889 similar to GP|15724256|gb|AAL06521.1 AT4g13010/F25G13_10... 25 6.7
AL375884 similar to PIR|T47959|T479 hypothetical protein F15G16.... 25 8.8
TC91652 similar to GP|11935197|gb|AAG42014.1 unknown protein {Ar... 25 8.8
TC90158 similar to GP|15724242|gb|AAL06514.1 At2g35500/T32F12.12... 25 8.8
BQ146586 similar to GP|22831296|dbj hypothetical protein~similar... 25 8.8
>BG581966 similar to GP|3776080|emb MtN9 {Medicago truncatula}, partial (53%)
Length = 683
Score = 176 bits (446), Expect = 2e-45
Identities = 93/133 (69%), Positives = 102/133 (75%), Gaps = 2/133 (1%)
Frame = +1
Query: 1 MTNVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVDDVVVGDTVIK--LG 58
M FRNAFTRWSQTTRVLNFSETTSYDDA+IKIGFY+I D VDDVVVGD+ I L
Sbjct: 7 MIEGFRNAFTRWSQTTRVLNFSETTSYDDADIKIGFYHIYNNDIVDDVVVGDSFISRNLD 186
Query: 59 SNVNSGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYP 118
SNV SG IRL SK+WV PT Y W+ EFDLET MHQIGHLLGLDHS DKE +MYP
Sbjct: 187 SNVKSGMIRLERSKFWVSPTTTYFKKWELQEFDLETVVMHQIGHLLGLDHSSDKESIMYP 366
Query: 119 AILPLQQQRKLQI 131
I PL Q++K+QI
Sbjct: 367 LIDPL-QEKKVQI 402
>TC86917 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago truncatula},
partial (59%)
Length = 1294
Score = 160 bits (406), Expect = 1e-40
Identities = 86/132 (65%), Positives = 98/132 (74%), Gaps = 1/132 (0%)
Frame = +1
Query: 1 MTNVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVDDVVVGDTVIKLGS- 59
MTNVFR A T+WS TTRVLNF+ET SYDDANIKIGFYNI DG++DV VG T I L S
Sbjct: 652 MTNVFRTALTQWSNTTRVLNFTETKSYDDANIKIGFYNITDDDGINDVAVGFTFIVLDST 831
Query: 60 NVNSGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPA 119
NV SGFI L A+KYW LPT ++ FDLETAAMHQIGHLLGL+HS D + +MYP
Sbjct: 832 NVKSGFITLDATKYWALPT-------EHRGFDLETAAMHQIGHLLGLEHSSDNKSIMYPT 990
Query: 120 ILPLQQQRKLQI 131
ILP Q+ +QI
Sbjct: 991 ILP-SHQKNVQI 1023
>TC86916 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago truncatula},
partial (39%)
Length = 954
Score = 82.0 bits (201), Expect(2) = 2e-33
Identities = 39/51 (76%), Positives = 44/51 (85%)
Frame = +2
Query: 1 MTNVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVDDVVVG 51
+TNVFR A TRWSQTTRVLNF+ETTSYDDA+IKI F N+ Y DG+ DVVVG
Sbjct: 581 VTNVFRKALTRWSQTTRVLNFTETTSYDDADIKIVFNNMTYDDGIYDVVVG 733
Score = 75.9 bits (185), Expect(2) = 2e-33
Identities = 39/66 (59%), Positives = 48/66 (72%)
Frame = +1
Query: 66 IRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAILPLQQ 125
I L +K+WV PT++ GE DLETAAMHQIGHLLGL+HS D + +MYP ILP Q
Sbjct: 781 ISLDITKHWVFPTED-------GELDLETAAMHQIGHLLGLEHSSDSKSIMYPTILP-SQ 936
Query: 126 QRKLQI 131
Q+K+QI
Sbjct: 937 QKKVQI 954
>TC79334 MtN9
Length = 1241
Score = 136 bits (343), Expect = 2e-33
Identities = 81/130 (62%), Positives = 88/130 (67%), Gaps = 1/130 (0%)
Frame = +1
Query: 3 NVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINY-IDGVDDVVVGDTVIKLGSNV 61
NVFRNAFTRWSQTTRVL FSE TSYDDA+IKIGFYNI+Y V DVVV D I N+
Sbjct: 439 NVFRNAFTRWSQTTRVLKFSEATSYDDADIKIGFYNISYNSKEVIDVVVSDFFI----NL 606
Query: 62 NSGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAIL 121
S IRL ASK W DLET AMHQIGHLLGLDHS D E +MYP I+
Sbjct: 607 RSFTIRLEASKVW----------------DLETVAMHQIGHLLGLDHSSDVESIMYPTIV 738
Query: 122 PLQQQRKLQI 131
PL Q+K+QI
Sbjct: 739 PL-HQKKVQI 765
>TC90008 weakly similar to GP|4249376|gb|AAD14473.1| Strong similarity to
GP|2829864 F3I6.6 zinc metalloproteinase homolog from
Arabidopsis thaliana BAC, partial (21%)
Length = 1173
Score = 95.5 bits (236), Expect = 5e-21
Identities = 53/129 (41%), Positives = 82/129 (63%)
Frame = +2
Query: 3 NVFRNAFTRWSQTTRVLNFSETTSYDDANIKIGFYNINYIDGVDDVVVGDTVIKLGSNVN 62
++F++AF RWS T LNF ET S++D++I+I F + DG V G + S+VN
Sbjct: 611 SLFQDAFNRWSNATE-LNFIETMSFNDSDIRIAFLTL---DGKGGTVGGSYI---NSSVN 769
Query: 63 SGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAILP 122
G + L A + WVLP++N + + + DLE+ MHQ+GHLLGL HS +E +MYP +L
Sbjct: 770 VGSVYLDADEQWVLPSENVV---EEDDVDLESVVMHQVGHLLGLGHSSVEEAIMYPIVL- 937
Query: 123 LQQQRKLQI 131
Q++K+++
Sbjct: 938 --QEKKIEL 958
>TC84571 weakly similar to SP|P29136|MEP1_SOYBN Metalloendoproteinase 1
precursor (EC 3.4.24.-) (SMEP1). [Soybean] {Glycine
max}, partial (28%)
Length = 506
Score = 47.0 bits (110), Expect = 2e-06
Identities = 25/58 (43%), Positives = 36/58 (61%)
Frame = +2
Query: 63 SGFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAI 120
+G L A++ WV+ D S DLE+ A+H+IGHLLGL HS ++E +M+P I
Sbjct: 14 NGRFHLDAAEDWVVSGDVSKSSLPTA-VDLESVAVHEIGHLLGLGHSSEEEAIMFPTI 184
>TC82218 similar to GP|7159629|emb|CAB76364.1 matrix metalloproteinase
{Cucumis sativus}, partial (19%)
Length = 688
Score = 46.6 bits (109), Expect = 3e-06
Identities = 19/30 (63%), Positives = 24/30 (79%)
Frame = +1
Query: 89 EFDLETAAMHQIGHLLGLDHSFDKEYVMYP 118
+FDLET A+H++GHLLGL HS D+ MYP
Sbjct: 256 QFDLETVALHEMGHLLGLAHSTDQNSAMYP 345
>TC81657 weakly similar to PIR|T51957|T51957 metalloproteinase (EC 3.4.24.-)
[imported] - Arabidopsis thaliana, partial (24%)
Length = 537
Score = 45.4 bits (106), Expect = 6e-06
Identities = 25/57 (43%), Positives = 34/57 (58%)
Frame = +2
Query: 64 GFIRLIASKYWVLPTDNYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAI 120
G + L ++ WV+ D S N DLE+ +H+IGHLLGL HS +E +MYP I
Sbjct: 8 GRLHLDKAEDWVVNGDVTESSLSNA-VDLESVVVHEIGHLLGLGHSSIEEAIMYPTI 175
>TC79163 weakly similar to PIR|E96724|E96724 hypothetical protein F20P5.11
[imported] - Arabidopsis thaliana, partial (32%)
Length = 762
Score = 32.0 bits (71), Expect = 0.072
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2
Query: 4 VFRNAFTRWSQTTRVLNFSETTSYDDANI 32
VF NAF +WS+ T + F+E TSY ++I
Sbjct: 677 VFANAFNQWSKVT-TITFTEATSYSSSDI 760
>TC87538 weakly similar to GP|21593148|gb|AAM65097.1 unknown {Arabidopsis
thaliana}, partial (63%)
Length = 1092
Score = 29.3 bits (64), Expect = 0.47
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +2
Query: 104 LGLDHSFDKEYVMYPAILPLQ 124
L + HSFDK Y MYP L L+
Sbjct: 107 LSIKHSFDKPYTMYPFFLLLE 169
>CA917134 weakly similar to PIR|E96724|E96 hypothetical protein F20P5.11
[imported] - Arabidopsis thaliana, partial (26%)
Length = 723
Score = 28.9 bits (63), Expect = 0.61
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +3
Query: 21 FSETTSYDDANIKIGFYNINYIDG 44
F+ETT Y A+IKIGF + ++ DG
Sbjct: 576 FTETTLYSGADIKIGFLHGDHGDG 647
>BQ148602 similar to GP|6729043|gb|A hypothetical protein {Arabidopsis
thaliana}, partial (5%)
Length = 692
Score = 27.3 bits (59), Expect = 1.8
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = +2
Query: 99 QIGHLLGLDHSFDKEYVMYPAILPLQ 124
QIG+LLG+ H +++Y +P + LQ
Sbjct: 197 QIGYLLGIGHGLERQY-YFPTLYSLQ 271
>TC88061 homologue to SP|Q43117|KPYA_RICCO Pyruvate kinase isozyme A
chloroplast precursor (EC 2.7.1.40). [Castor bean]
{Ricinus communis}, partial (84%)
Length = 1765
Score = 26.6 bits (57), Expect = 3.0
Identities = 21/67 (31%), Positives = 27/67 (39%), Gaps = 7/67 (10%)
Frame = +3
Query: 38 NINYIDGVDDVVVGDTVIKLGSNVNSGFIRLIASKYWVLPTD-------NYMWSWQNGEF 90
N+NY +DV VGD ++ G V I I L TD + W+NG
Sbjct: 684 NVNYEGFAEDVKVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRNGSL 863
Query: 91 DLETAAM 97
E AM
Sbjct: 864 VRERNAM 884
>TC92804
Length = 481
Score = 25.8 bits (55), Expect = 5.2
Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 7/56 (12%)
Frame = +3
Query: 52 DTVIKLGSNVNSGFIRLIASKYWVLPTDNYMW-------SWQNGEFDLETAAMHQI 100
+ +IKL S + S F+RL Y +L DN + SW N F L+ + +
Sbjct: 27 NNIIKLRSILFSLFLRLHGRNYSLLWNDNKSYKLGVEGISWMNSLFGLQCLLRYHV 194
>BG645531
Length = 790
Score = 25.8 bits (55), Expect = 5.2
Identities = 14/29 (48%), Positives = 18/29 (61%), Gaps = 1/29 (3%)
Frame = +1
Query: 13 SQTTRVLNFSETTSYDDANI-KIGFYNIN 40
SQT +LN SY DAN+ +I + NIN
Sbjct: 85 SQTNMLLNGCNIFSYTDANMRRISYENIN 171
>TC90889 similar to GP|15724256|gb|AAL06521.1 AT4g13010/F25G13_100
{Arabidopsis thaliana}, partial (69%)
Length = 779
Score = 25.4 bits (54), Expect = 6.7
Identities = 12/39 (30%), Positives = 18/39 (45%)
Frame = -1
Query: 82 MWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAI 120
+WSW L++ IG +L H FD + Y +I
Sbjct: 140 LWSWNMNFNMLKSRRTSIIGIVLNCMHEFDSHVIYYLSI 24
>AL375884 similar to PIR|T47959|T479 hypothetical protein F15G16.60 -
Arabidopsis thaliana, partial (5%)
Length = 523
Score = 25.0 bits (53), Expect = 8.8
Identities = 13/35 (37%), Positives = 16/35 (45%), Gaps = 1/35 (2%)
Frame = -3
Query: 4 VFRNAFTRWSQTTRV-LNFSETTSYDDANIKIGFY 37
+F N W R LNF +TT Y N GF+
Sbjct: 419 IFTNQILHWIHDLRYHLNFPKTT*YQAQNF*CGFF 315
>TC91652 similar to GP|11935197|gb|AAG42014.1 unknown protein {Arabidopsis
thaliana}, partial (78%)
Length = 887
Score = 25.0 bits (53), Expect = 8.8
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +2
Query: 103 LLGLDHSFDKEYVMYPAILPLQQQ 126
LLGLDH+F +++ A+L LQQQ
Sbjct: 281 LLGLDHAFVMPFLLKVALL-LQQQ 349
>TC90158 similar to GP|15724242|gb|AAL06514.1 At2g35500/T32F12.12
{Arabidopsis thaliana}, partial (36%)
Length = 764
Score = 25.0 bits (53), Expect = 8.8
Identities = 16/49 (32%), Positives = 22/49 (44%)
Frame = +2
Query: 80 NYMWSWQNGEFDLETAAMHQIGHLLGLDHSFDKEYVMYPAILPLQQQRK 128
N + SW +G HLL HSF KE+ ++PL+ RK
Sbjct: 458 NGLTSWNHGN------------HLLLDHHSF*KEHPSILLVIPLKSTRK 568
>BQ146586 similar to GP|22831296|dbj hypothetical protein~similar to
Arabidopsis thaliana chromosome2 At2g36110, partial
(4%)
Length = 320
Score = 25.0 bits (53), Expect = 8.8
Identities = 11/41 (26%), Positives = 24/41 (57%), Gaps = 2/41 (4%)
Frame = -2
Query: 39 INYIDGVDDVVVGDTV--IKLGSNVNSGFIRLIASKYWVLP 77
IN++ ++++ T +K ++NS + LI K+W++P
Sbjct: 178 INHLQSFNNLMRIKTTSNLKGKKSINSSILNLIPIKHWIIP 56
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.138 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,033,762
Number of Sequences: 36976
Number of extensions: 50739
Number of successful extensions: 269
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 263
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of query: 131
length of database: 9,014,727
effective HSP length: 85
effective length of query: 46
effective length of database: 5,871,767
effective search space: 270101282
effective search space used: 270101282
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144345.5