BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144345.16 + phase: 0 /pseudo/partial
(221 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fra... 65 2e-11
BQ150350 32 0.15
TC78468 similar to GP|21594872|gb|AAM66051.1 unknown {Arabidopsi... 30 0.56
TC87536 similar to GP|21553686|gb|AAM62779.1 unknown {Arabidopsi... 29 1.3
TC88861 weakly similar to GP|17027283|gb|AAL34137.1 putative pro... 29 1.3
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 29 1.6
TC86724 homologue to PIR|T06445|T06445 GTP-binding protein - gar... 29 1.6
TC85049 weakly similar to GP|15810369|gb|AAL07072.1 unknown prot... 28 2.8
TC76683 similar to SP|Q41140|PFPA_RICCO Pyrophosphate--fructose ... 28 3.6
TC91111 similar to GP|21554235|gb|AAM63310.1 CPRD49 {Arabidopsis... 27 4.8
BQ158073 similar to SP|Q42807|STAD_ Acyl-[acyl-carrier protein] ... 27 4.8
TC80960 GP|13361096|dbj|BAB35055. portal protein {Escherichia co... 27 6.2
BI310177 GP|13361096|d portal protein {Escherichia coli O157:H7}... 27 6.2
TC80173 similar to GP|9758428|dbj|BAB09014.1 contains similarity... 27 6.2
TC82689 similar to PIR|T51440|T51440 alpha-mannosidase-like prot... 27 6.2
TC78816 similar to GP|14190377|gb|AAK55669.1 AT4g00850/A_TM018A1... 27 6.2
TC86804 similar to GP|5725342|emb|CAA63650.1 ribosomal protein S... 27 6.2
TC84234 similar to PIR|D96586|D96586 hypothetical protein F20D21... 27 6.2
TC80810 homologue to PIR|T46203|T46203 transcription factor Hap5... 27 8.1
TC77932 similar to GP|15289779|dbj|BAB63479. putative t-SNARE SE... 27 8.1
>CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fragment),
partial (9%)
Length = 624
Score = 65.1 bits (157), Expect = 2e-11
Identities = 34/57 (59%), Positives = 39/57 (67%)
Frame = -3
Query: 164 RFNNNNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLDRASLHLIRCLQGSGLISNV 220
RF NN+ ++L L L Y+CYMLS +LY EG Q DR SLHLIR L GSGL SNV
Sbjct: 517 RFTNNDSSNRLDLPFL*YQCYMLSSSRLYFSEGTAQSDRGSLHLIRYLPGSGLTSNV 347
>BQ150350
Length = 1064
Score = 32.3 bits (72), Expect = 0.15
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = -2
Query: 110 LLLLSMQLKCLRHTHICRILSIRSSHRFIISTL 142
L LL++QL LRH I + I + H +IISTL
Sbjct: 952 LRLLNLQLHLLRHHFIATVFRITNIHSYIISTL 854
>TC78468 similar to GP|21594872|gb|AAM66051.1 unknown {Arabidopsis
thaliana}, partial (78%)
Length = 1249
Score = 30.4 bits (67), Expect = 0.56
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = -1
Query: 71 CLRKLNLLQVRRRGMVMDTTRRKKLKWGWCQLEQVNQWLLLLLSMQLKCL 120
CLRKL Q+RRR + + +R + K ++ N W+L LL MQ L
Sbjct: 289 CLRKLT*NQLRRRRVNLRMVKRPREK----RMRNQNGWVLSLLIMQQSML 152
>TC87536 similar to GP|21553686|gb|AAM62779.1 unknown {Arabidopsis thaliana},
partial (94%)
Length = 1358
Score = 29.3 bits (64), Expect = 1.3
Identities = 17/52 (32%), Positives = 23/52 (43%), Gaps = 6/52 (11%)
Frame = +1
Query: 95 LKWGWCQL------EQVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIIS 140
L WGW L E+ LLL M+L C H C I+ + + F+ S
Sbjct: 1015 LVWGWLDLR*RLDSEEEKMCNKLLLIMKLSCYPHHLFCLIMYFKFDYLFLSS 1170
>TC88861 weakly similar to GP|17027283|gb|AAL34137.1 putative protein kinase
{Oryza sativa (japonica cultivar-group)}, partial (34%)
Length = 1847
Score = 29.3 bits (64), Expect = 1.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +3
Query: 119 CLRHTHICRILSIRSSHRFIISTLCRRASL 148
C+RH CR + +S R II T R SL
Sbjct: 1431 CIRHCQACRFWGVENSDREII*TFAERKSL 1520
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100
- Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 28.9 bits (63), Expect = 1.6
Identities = 22/86 (25%), Positives = 42/86 (48%), Gaps = 1/86 (1%)
Frame = +1
Query: 34 R*RKIMLRG*SSLPRTTFRRWSLWEPVLRKPSGMELLCLRKLNLLQVRRRGMVMDTTRRK 93
R* K+++R + R W + RK ++ R++ ++R+RG+ + TTR
Sbjct: 637 R*MKMIMRNQRQIMIVRMRLW-----MKRKTKKKRVMMRRRMKTRRMRKRGV*LKTTRIM 801
Query: 94 KLKWGWCQLEQ-VNQWLLLLLSMQLK 118
KL +L + W ++L+ + LK
Sbjct: 802 KLGKNITRLMMPLVLWHMILMKLALK 879
>TC86724 homologue to PIR|T06445|T06445 GTP-binding protein - garden pea,
complete
Length = 1037
Score = 28.9 bits (63), Expect = 1.6
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = -2
Query: 137 FIISTLCRRASLKCPSMRLPNR*NNRCRFNNNNKISKLG 175
+II+ +C CPS+R N+ N +C N+ +SK G
Sbjct: 1009 YIITFIC------CPSLRNSNKLNFKCTKGNSPNLSKFG 911
>TC85049 weakly similar to GP|15810369|gb|AAL07072.1 unknown protein
{Arabidopsis thaliana}, partial (8%)
Length = 819
Score = 28.1 bits (61), Expect = 2.8
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +1
Query: 119 CLRHTHICRILSIRSSHRFIISTLCRRAS 147
CLRH H R+L R RF++ +CR+ S
Sbjct: 466 CLRHLHRRRVLQCR---RFLLLLVCRKQS 543
>TC76683 similar to SP|Q41140|PFPA_RICCO Pyrophosphate--fructose 6-phosphate
1-phosphotransferase alpha subunit (EC 2.7.1.90) (PFP),
partial (98%)
Length = 2413
Score = 27.7 bits (60), Expect = 3.6
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 90 TRRKKLKWGWCQLEQVNQWLLLLLSMQLKC 119
TR KLKW W + V++ +LS QL C
Sbjct: 1722 TRTIKLKWAWISINIVHKKACRILS*QLIC 1633
>TC91111 similar to GP|21554235|gb|AAM63310.1 CPRD49 {Arabidopsis thaliana},
partial (38%)
Length = 682
Score = 27.3 bits (59), Expect = 4.8
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -1
Query: 110 LLLLSMQLKCLRHTHICRILSIRSSHRFII 139
++LL+ C+ H +ICR L R + F I
Sbjct: 406 IILLTTYFNCVEHLNICRYLKSR*QYSFTI 317
>BQ158073 similar to SP|Q42807|STAD_ Acyl-[acyl-carrier protein] desaturase
chloroplast precursor (EC 1.14.99.6), partial (7%)
Length = 932
Score = 27.3 bits (59), Expect = 4.8
Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 6/37 (16%)
Frame = -3
Query: 118 KCLRHTHICRILSIRSSHRFII------STLCRRASL 148
+C+ TH+ ++ I S+H+ ++ STLC R S+
Sbjct: 696 RCVSDTHVAAMIFIISTHKVVVDRVT*FSTLCLRISV 586
>TC80960 GP|13361096|dbj|BAB35055. portal protein {Escherichia coli
O157:H7}, partial (47%)
Length = 1084
Score = 26.9 bits (58), Expect = 6.2
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +3
Query: 46 LPRTTFRRWSLWEPVLRKPSG 66
LP + +RWS +PVL +P G
Sbjct: 633 LPSRSVKRWSAVQPVLNRPPG 695
>BI310177 GP|13361096|d portal protein {Escherichia coli O157:H7}, partial
(22%)
Length = 743
Score = 26.9 bits (58), Expect = 6.2
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +1
Query: 46 LPRTTFRRWSLWEPVLRKPSG 66
LP + +RWS +PVL +P G
Sbjct: 652 LPSKSVKRWSAVQPVLNRPPG 714
>TC80173 similar to GP|9758428|dbj|BAB09014.1 contains similarity to unknown
protein~gene_id:K8K14.1~sp|Q10058 {Arabidopsis
thaliana}, partial (76%)
Length = 1375
Score = 26.9 bits (58), Expect = 6.2
Identities = 17/46 (36%), Positives = 23/46 (49%), Gaps = 6/46 (13%)
Frame = +1
Query: 100 CQLEQVNQWLLLLLSMQLKCLRHTH------ICRILSIRSSHRFII 139
C + ++ L LLS Q +CLRHTH +CR R RF +
Sbjct: 115 CLRRRSHRRLHCLLSHQERCLRHTHREIHHRLCRFR*SRWISRFTL 252
>TC82689 similar to PIR|T51440|T51440 alpha-mannosidase-like protein -
Arabidopsis thaliana, partial (16%)
Length = 786
Score = 26.9 bits (58), Expect = 6.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +2
Query: 105 VNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFI 138
V Q ++ L + +L+C+ H CR L R S F+
Sbjct: 128 VLQKIMWLWTTELECIPHYKTCRFLCRRESRHFL 229
>TC78816 similar to GP|14190377|gb|AAK55669.1 AT4g00850/A_TM018A10_22
{Arabidopsis thaliana}, partial (43%)
Length = 1150
Score = 26.9 bits (58), Expect = 6.2
Identities = 17/48 (35%), Positives = 24/48 (49%), Gaps = 3/48 (6%)
Frame = +2
Query: 100 CQLEQVNQWLLLLLSMQLKCLRHTHI---CRILSIRSSHRFIISTLCR 144
C + ++ QWL KC + I CRI SI S++ IS LC+
Sbjct: 479 CNILRLQQWLSNKECSPKKCQCSSVIRIKCRISSISSNNSSYISKLCK 622
>TC86804 similar to GP|5725342|emb|CAA63650.1 ribosomal protein S5 {Spinacia
oleracea}, partial (75%)
Length = 1353
Score = 26.9 bits (58), Expect = 6.2
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = +2
Query: 69 LLCLRKLNLLQVRRRGMVMDTT---RRKKLKWGW 99
L+CL + + +RRRG + D+ RRK+ + GW
Sbjct: 461 LVCLEMI*M*WIRRRGKIPDSVRV*RRKR*EMGW 562
>TC84234 similar to PIR|D96586|D96586 hypothetical protein F20D21.27
[imported] - Arabidopsis thaliana, partial (19%)
Length = 721
Score = 26.9 bits (58), Expect = 6.2
Identities = 16/55 (29%), Positives = 27/55 (49%)
Frame = +2
Query: 100 CQLEQVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIISTLCRRASLKCPSMR 154
C+L N +++LLL ++ C T S H F +++C + CPS+R
Sbjct: 92 CKLYIANSFIILLLRSRVPCSIST------SKSQPHFFYPASICVH*NSPCPSVR 238
>TC80810 homologue to PIR|T46203|T46203 transcription factor Hap5a -
Arabidopsis thaliana, partial (68%)
Length = 942
Score = 26.6 bits (57), Expect = 8.1
Identities = 10/21 (47%), Positives = 12/21 (56%)
Frame = -2
Query: 93 KKLKWGWCQLEQVNQWLLLLL 113
K L+W WC W+LLLL
Sbjct: 98 KMLEWWWCNCRG*RCWILLLL 36
>TC77932 similar to GP|15289779|dbj|BAB63479. putative t-SNARE SED5 {Oryza
sativa (japonica cultivar-group)}, partial (71%)
Length = 1431
Score = 26.6 bits (57), Expect = 8.1
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = -1
Query: 79 QVRRRGMVMDTTRRKKLKWGWCQLEQVNQWLLLLLSMQ 116
Q+ R + +RKK W W +LE + L+ +S+Q
Sbjct: 372 QIEDRRIWKKKGKRKKEHWWWLRLELIQMKLISSISLQ 259
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.343 0.149 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,859,071
Number of Sequences: 36976
Number of extensions: 157164
Number of successful extensions: 1951
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1932
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1949
length of query: 221
length of database: 9,014,727
effective HSP length: 92
effective length of query: 129
effective length of database: 5,612,935
effective search space: 724068615
effective search space used: 724068615
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144345.16