BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143341.1 + phase: 0 /partial
(249 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AJ498385 similar to GP|9759547|dbj DnaJ homologue {Arabidopsis t... 412 e-116
TC92801 similar to GP|9294116|dbj|BAB01967.1 dnaJ protein-like {... 229 e-101
BF631720 weakly similar to GP|10798648|em putative DNAJ protein ... 79 2e-15
TC87441 similar to GP|21928031|gb|AAM78044.1 At3g62600/F26K9_30 ... 73 1e-13
BG647719 similar to GP|10177754|dbj DnaJ protein-like {Arabidops... 72 3e-13
TC77797 similar to GP|6714354|gb|AAF26045.1| ARG1 protein (Alter... 70 8e-13
TC90074 similar to PIR|G84590|G84590 probable heat shock protein... 68 3e-12
TC90148 similar to GP|6403503|gb|AAF07843.1| putative DnaJ prote... 67 8e-12
TC93716 similar to GP|6179940|gb|AAF05720.1| DnaJ-like protein {... 66 1e-11
TC90708 weakly similar to PIR|S26703|S26703 dnaJ protein homolog... 66 1e-11
TC82757 similar to PIR|T08563|T08563 dnaJ-related protein T22F8.... 66 1e-11
TC87379 similar to PIR|T04618|T04618 heat shock protein homolog ... 65 2e-11
TC86482 similar to GP|9294648|dbj|BAB02987.1 gene_id:MAG2.19~unk... 65 2e-11
TC90060 similar to GP|23428796|gb|AAM23263.1 DnaJ-like protein {... 64 7e-11
TC78760 similar to PIR|T48161|T48161 heat shock protein 40-like ... 64 7e-11
TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock... 64 7e-11
TC86700 similar to GP|18377835|gb|AAL67104.1 At1g24120/F3I6_4 {A... 63 9e-11
TC77020 similar to PIR|D96795|D96795 probable DnaJ protein 1979... 63 9e-11
TC85313 PIR|T09338|T09338 DnaJ-like protein MsJ1 - alfalfa, comp... 62 3e-10
TC77858 similar to GP|20197886|gb|AAD22362.2 putative DnaJ prote... 61 5e-10
>AJ498385 similar to GP|9759547|dbj DnaJ homologue {Arabidopsis thaliana},
partial (70%)
Length = 711
Score = 412 bits (1060), Expect = e-116
Identities = 205/206 (99%), Positives = 206/206 (99%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ
Sbjct: 94 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 273
Query: 61 TGCIDDDDLAGDVQNLHAFFKTMYKKVTEADIEEFEANYRGSDSEKNDLIELYKKYKGNM 120
TGCIDDDDLAGDVQNLHAFFKTMYKKVTEADIEEFEANYRGSDSEKNDLIELYKKYKGNM
Sbjct: 274 TGCIDDDDLAGDVQNLHAFFKTMYKKVTEADIEEFEANYRGSDSEKNDLIELYKKYKGNM 453
Query: 121 KRLFCSMLCSDAKLDSHRFKDILDEAIAAGELKATKAYQKWAKEVSETKPPTSPLKRKAK 180
KRLFCSMLCSDAKLDSHRFKDILDEAIAAGELKATKAYQKWAKEVSETKPPTSPLKRKAK
Sbjct: 454 KRLFCSMLCSDAKLDSHRFKDILDEAIAAGELKATKAYQKWAKEVSETKPPTSPLKRKAK 633
Query: 181 SNKQSETDLCAIISQRRNERKGQFDS 206
SNKQSETDLCAIISQRRNER+GQFDS
Sbjct: 634 SNKQSETDLCAIISQRRNERRGQFDS 711
>TC92801 similar to GP|9294116|dbj|BAB01967.1 dnaJ protein-like {Arabidopsis
thaliana}, partial (84%)
Length = 829
Score = 229 bits (583), Expect(2) = e-101
Identities = 113/135 (83%), Positives = 124/135 (91%), Gaps = 1/135 (0%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+EKTA++QEIKKAY+KLALRLHPDKNPGDEEAK KFQQLQ VISILGDEEKRALYD+
Sbjct: 104 VLGVEKTATKQEIKKAYHKLALRLHPDKNPGDEEAKAKFQQLQNVISILGDEEKRALYDE 283
Query: 61 TGCIDDDDLAGD-VQNLHAFFKTMYKKVTEADIEEFEANYRGSDSEKNDLIELYKKYKGN 119
TGC+D DLAGD VQ+L +F+T+YKKVTEADIEEFEANYRGSDSEKNDLI LYK+ KGN
Sbjct: 284 TGCVDHADLAGDVVQDLQEYFRTLYKKVTEADIEEFEANYRGSDSEKNDLIRLYKECKGN 463
Query: 120 MKRLFCSMLCSDAKL 134
M RLFCSMLCSD KL
Sbjct: 464 MNRLFCSMLCSDPKL 508
Score = 158 bits (399), Expect(2) = e-101
Identities = 76/106 (71%), Positives = 92/106 (86%)
Frame = +3
Query: 135 DSHRFKDILDEAIAAGELKATKAYQKWAKEVSETKPPTSPLKRKAKSNKQSETDLCAIIS 194
DSHRFKDI+DEAI +GELK KAY+KWAK++SETKPPTSPL+RK KS+K+ +TDL AIIS
Sbjct: 510 DSHRFKDIIDEAIPSGELKEKKAYKKWAKKISETKPPTSPLRRKQKSSKEPKTDLYAIIS 689
Query: 195 QRRNERKGQFDSMFASLVSKYGGSDMPEPSEEEFEAAQKKLEKGRS 240
+RR+ERK +FDSMF+SL+SKYGGS +PEPSE E A QKK+E RS
Sbjct: 690 KRRDERKDRFDSMFSSLISKYGGSHVPEPSEREI*ATQKKMESRRS 827
>BF631720 weakly similar to GP|10798648|em putative DNAJ protein {Nicotiana
tabacum}, partial (25%)
Length = 630
Score = 78.6 bits (192), Expect = 2e-15
Identities = 34/61 (55%), Positives = 48/61 (77%)
Frame = +3
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQT 61
LG++ ASQ EIKKAY KLA++ HPDKNPG++EA+E F+++ + +L DE+KR +YDQ
Sbjct: 78 LGVQPNASQDEIKKAYRKLAVKYHPDKNPGNKEAEEMFKKISEAYEVLSDEQKRQMYDQY 257
Query: 62 G 62
G
Sbjct: 258 G 260
>TC87441 similar to GP|21928031|gb|AAM78044.1 At3g62600/F26K9_30
{Arabidopsis thaliana}, partial (94%)
Length = 1563
Score = 72.8 bits (177), Expect = 1e-13
Identities = 32/62 (51%), Positives = 44/62 (70%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
+L + K AS +IK+AY KLAL+ HPDKNPG+EEA +KF ++ +L D EKR +YD+
Sbjct: 275 ILQVSKGASDDQIKRAYRKLALKYHPDKNPGNEEANKKFAEISNAYEVLSDNEKRNIYDK 454
Query: 61 TG 62
G
Sbjct: 455 YG 460
>BG647719 similar to GP|10177754|dbj DnaJ protein-like {Arabidopsis
thaliana}, partial (16%)
Length = 702
Score = 71.6 bits (174), Expect = 3e-13
Identities = 35/62 (56%), Positives = 42/62 (67%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ K AS EIKKAYY LA +LHPD N D EA++KFQ++ +L D EKR YDQ
Sbjct: 270 VLGVSKDASSSEIKKAYYGLAKKLHPDANKDDPEAEKKFQEVTLAYEVLKDGEKRQQYDQ 449
Query: 61 TG 62
G
Sbjct: 450 VG 455
>TC77797 similar to GP|6714354|gb|AAF26045.1| ARG1 protein (Altered Response
to Gravity); 32591-35072 {Arabidopsis thaliana}, partial
(93%)
Length = 2063
Score = 70.1 bits (170), Expect = 8e-13
Identities = 41/93 (44%), Positives = 56/93 (60%), Gaps = 7/93 (7%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ K +S QEIK AY KLAL+ HPDKN + EA E F+++ +IL D EKR YD
Sbjct: 426 VLGVSKESSDQEIKTAYRKLALKYHPDKNANNPEASELFKEVAYSYNILSDPEKRRQYDS 605
Query: 61 TG--CIDDD--DLAGDVQNL---HAFFKTMYKK 86
G +D D D+ D+ NL + F ++K+
Sbjct: 606 AGFEALDADSMDMEIDLSNLGTVNTMFAALFKQ 704
>TC90074 similar to PIR|G84590|G84590 probable heat shock protein
[imported] - Arabidopsis thaliana, partial (88%)
Length = 1287
Score = 68.2 bits (165), Expect = 3e-12
Identities = 31/74 (41%), Positives = 54/74 (72%), Gaps = 1/74 (1%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD-EEAKEKFQQLQKVISILGDEEKRALYD 59
+L ++K A+++E+KKAY KLA++ HPDKNP + ++A+ KF+++ + +L D +K+A+YD
Sbjct: 22 ILKVDKNATEEELKKAYRKLAMKWHPDKNPSNKKDAEAKFKEISEAYEVLSDPQKKAIYD 201
Query: 60 QTGCIDDDDLAGDV 73
Q G ++ L G V
Sbjct: 202QYG---EEGLKGQV 234
>TC90148 similar to GP|6403503|gb|AAF07843.1| putative DnaJ protein
{Arabidopsis thaliana}, partial (36%)
Length = 894
Score = 66.6 bits (161), Expect = 8e-12
Identities = 33/62 (53%), Positives = 46/62 (73%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG++K+ASQ+EI+KA++KL+L+ HPDKN + A+EKF Q+ IL DE+KR YD
Sbjct: 161 VLGVDKSASQREIQKAFHKLSLQYHPDKNKA-KGAQEKFAQINNAYEILSDEQKRKNYDL 337
Query: 61 TG 62
G
Sbjct: 338 YG 343
>TC93716 similar to GP|6179940|gb|AAF05720.1| DnaJ-like protein {Nicotiana
tabacum}, partial (33%)
Length = 571
Score = 66.2 bits (160), Expect = 1e-11
Identities = 32/74 (43%), Positives = 50/74 (67%), Gaps = 1/74 (1%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD-EEAKEKFQQLQKVISILGDEEKRALYD 59
+L +++ A ++KKAY KLA++ HPDKNP + +EA+ KF+Q+ + +L D KRA+YD
Sbjct: 152 LLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDSNKRAVYD 331
Query: 60 QTGCIDDDDLAGDV 73
Q G +D L G +
Sbjct: 332 QYG---EDGLKGQM 364
>TC90708 weakly similar to PIR|S26703|S26703 dnaJ protein homolog YDJ1 -
yeast (Saccharomyces cerevisiae), partial (43%)
Length = 1098
Score = 65.9 bits (159), Expect = 1e-11
Identities = 30/61 (49%), Positives = 43/61 (70%)
Frame = +2
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQT 61
LG++ A+ QE++KAY AL+ HPDKN + EA+EKF+++ IL D +KRA+YDQ
Sbjct: 287 LGVKPEANDQELRKAYKTNALKYHPDKNAHNPEAEEKFKEISHAYEILSDSQKRAVYDQY 466
Query: 62 G 62
G
Sbjct: 467 G 469
>TC82757 similar to PIR|T08563|T08563 dnaJ-related protein T22F8.50 -
Arabidopsis thaliana, partial (95%)
Length = 1317
Score = 65.9 bits (159), Expect = 1e-11
Identities = 32/61 (52%), Positives = 39/61 (63%)
Frame = +2
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQT 61
LG+ AS +IKKAYY A +HPDKNPGD +A E FQ L + +L D EKR YD+
Sbjct: 125 LGVSVDASAADIKKAYYVKARIVHPDKNPGDPKAAENFQLLGEAYQVLSDPEKREAYDKN 304
Query: 62 G 62
G
Sbjct: 305 G 307
>TC87379 similar to PIR|T04618|T04618 heat shock protein homolog F20O9.160 -
Arabidopsis thaliana, partial (75%)
Length = 1431
Score = 65.1 bits (157), Expect = 2e-11
Identities = 29/63 (46%), Positives = 46/63 (72%), Gaps = 1/63 (1%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDE-EAKEKFQQLQKVISILGDEEKRALYD 59
+L ++K A+ E+KKAY KLA++ HPDKNP ++ +A+ KF+ + + +L D +KRA+YD
Sbjct: 104 ILEVDKNATDDELKKAYRKLAMKWHPDKNPDNKNDAETKFKLISEAYEVLSDPQKRAIYD 283
Query: 60 QTG 62
Q G
Sbjct: 284 QYG 292
>TC86482 similar to GP|9294648|dbj|BAB02987.1 gene_id:MAG2.19~unknown
protein {Arabidopsis thaliana}, partial (38%)
Length = 718
Score = 65.1 bits (157), Expect = 2e-11
Identities = 39/100 (39%), Positives = 52/100 (52%), Gaps = 6/100 (6%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD------EEAKEKFQQLQKVISILGDEEK 54
VLGL K S E++ AY KLAL+ HPD+ EEAK+KFQ +Q+ S+L D K
Sbjct: 428 VLGLNKECSDSELRNAYKKLALKWHPDRCSASGNVKFVEEAKKKFQAIQEAYSVLSDSNK 607
Query: 55 RALYDQTGCIDDDDLAGDVQNLHAFFKTMYKKVTEADIEE 94
R +YD G D DD D + F M +++ E
Sbjct: 608 RLMYD-VGVYDSDD---DENGMGDFLNEMVTMMSQTKSNE 715
>TC90060 similar to GP|23428796|gb|AAM23263.1 DnaJ-like protein {Glycine
max}, partial (90%)
Length = 804
Score = 63.5 bits (153), Expect = 7e-11
Identities = 33/63 (52%), Positives = 43/63 (67%), Gaps = 4/63 (6%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDK---NPGDE-EAKEKFQQLQKVISILGDEEKRA 56
VLG+ AS +I+ AY KLA+R HPDK NP EAK +FQQ+Q+ S+L DE KR+
Sbjct: 250 VLGIRSDASSSDIRTAYRKLAMRWHPDKFARNPTTAGEAKRRFQQIQEAYSVLSDESKRS 429
Query: 57 LYD 59
+YD
Sbjct: 430 MYD 438
>TC78760 similar to PIR|T48161|T48161 heat shock protein 40-like -
Arabidopsis thaliana, partial (56%)
Length = 762
Score = 63.5 bits (153), Expect = 7e-11
Identities = 28/63 (44%), Positives = 45/63 (70%), Gaps = 1/63 (1%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD-EEAKEKFQQLQKVISILGDEEKRALYD 59
VL +++ A ++KKAY KLA++ HPDKNP + ++A+ KF+ + + +L D +KRA+YD
Sbjct: 36 VLQIDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKDAEAKFKTISEAYDVLSDPQKRAVYD 215
Query: 60 QTG 62
Q G
Sbjct: 216QYG 224
>TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock protein
{Arabidopsis thaliana}, partial (89%)
Length = 1344
Score = 63.5 bits (153), Expect = 7e-11
Identities = 28/63 (44%), Positives = 45/63 (70%), Gaps = 1/63 (1%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD-EEAKEKFQQLQKVISILGDEEKRALYD 59
VL +++ A ++KKAY KLA++ HPDKNP + ++A+ KF+ + + +L D +KRA+YD
Sbjct: 90 VLQIDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKDAEAKFKTISEAYDVLSDPQKRAVYD 269
Query: 60 QTG 62
Q G
Sbjct: 270 QYG 278
>TC86700 similar to GP|18377835|gb|AAL67104.1 At1g24120/F3I6_4 {Arabidopsis
thaliana}, partial (83%)
Length = 1929
Score = 63.2 bits (152), Expect = 9e-11
Identities = 35/94 (37%), Positives = 54/94 (57%), Gaps = 7/94 (7%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ + ++ QEIK AY KLAL+ HPDKN D +A + F++ ++L D +KR YD
Sbjct: 280 VLGVSRNSTDQEIKSAYRKLALKFHPDKNANDPKAADLFKEATFSYNLLSDPDKRRQYDS 459
Query: 61 TGC----IDDDDLAGDVQNLHA---FFKTMYKKV 87
+G D +L D+ +L A F ++ K+
Sbjct: 460 SGFEAVESDSQELELDLSSLGAVNTMFAALFSKL 561
>TC77020 similar to PIR|D96795|D96795 probable DnaJ protein 19794-17391
[imported] - Arabidopsis thaliana, partial (80%)
Length = 1848
Score = 63.2 bits (152), Expect = 9e-11
Identities = 31/62 (50%), Positives = 39/62 (62%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ TAS+ EIKKAYY A ++HPDKNP D A + FQ L + +L D +R YD
Sbjct: 240 VLGVSPTASEAEIKKAYYIKARQVHPDKNPNDPLAAQNFQVLGEAYQVLSDPTQRQAYDA 419
Query: 61 TG 62
G
Sbjct: 420 HG 425
>TC85313 PIR|T09338|T09338 DnaJ-like protein MsJ1 - alfalfa, complete
Length = 1927
Score = 61.6 bits (148), Expect = 3e-10
Identities = 32/62 (51%), Positives = 43/62 (68%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
+LG+ K+AS+ EIKKAY K A++ HPDK GD EKF++L + +L D EK+ LYDQ
Sbjct: 331 ILGVSKSASEDEIKKAYRKAAMKNHPDKG-GD---PEKFKELGQAYEVLSDPEKKELYDQ 498
Query: 61 TG 62
G
Sbjct: 499 YG 504
>TC77858 similar to GP|20197886|gb|AAD22362.2 putative DnaJ protein
{Arabidopsis thaliana}, partial (78%)
Length = 1869
Score = 60.8 bits (146), Expect = 5e-10
Identities = 29/62 (46%), Positives = 42/62 (66%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ K +++ EIK AY KLA HPD N D A+EKF+++ +L D+EKR++YD+
Sbjct: 510 VLGVSKNSTKSEIKTAYRKLARNYHPDVNK-DPGAEEKFKEISNAYEVLSDDEKRSIYDK 686
Query: 61 TG 62
G
Sbjct: 687 YG 692
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.128 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,023,334
Number of Sequences: 36976
Number of extensions: 66799
Number of successful extensions: 441
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 421
length of query: 249
length of database: 9,014,727
effective HSP length: 94
effective length of query: 155
effective length of database: 5,538,983
effective search space: 858542365
effective search space used: 858542365
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC143341.1