BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142143.6 - phase: 0 /pseudo
(175 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 77 5e-15
BF006686 33 0.044
TC90620 similar to GP|14334592|gb|AAK59474.1 unknown protein {Ar... 29 1.1
BF649019 similar to PIR|T49065|T49 hypothetical protein T5P19.29... 28 1.9
TC83624 homologue to PIR|G84581|G84581 copia-like retroelement p... 28 1.9
TC91288 similar to PIR|T51354|T51354 cyclic nucleotide-regulated... 27 3.2
BF641241 homologue to GP|14279070|dbj tRNA isopentenyltransferas... 26 7.1
TC80325 homologue to PIR|F84674|F84674 probable AAA-type ATPase ... 26 9.2
BI270951 homologue to GP|20906098|gb Cobalamin biosynthesis prot... 26 9.2
TC90743 GP|16025115|gb|AAL11411.1 major histocompatibility class... 26 9.2
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 77.4 bits (189), Expect(2) = 5e-15
Identities = 40/69 (57%), Positives = 53/69 (75%), Gaps = 1/69 (1%)
Frame = +2
Query: 3 QMGKVIFLYLGLPIGGDLSRLMFWEPVLDRIKKRLSRC-SKFLSFGGRMVLFKFVLSFIH 61
++GKV FLYLG+PI G+ RL FWEP+++RIK RL+ S+FLSFGGR+VL K VL+ +
Sbjct: 737 KVGKVPFLYLGMPIEGNSRRLSFWEPIVNRIKARLTGWNSRFLSFGGRLVLLKSVLTSLS 916
Query: 62 VYALSFFKV 70
VYAL K+
Sbjct: 917 VYALPSSKL 943
Score = 19.2 bits (38), Expect(2) = 5e-15
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = +1
Query: 66 SFFKVPSG 73
SFFK PSG
Sbjct: 928 SFFKAPSG 951
>BF006686
Length = 325
Score = 33.5 bits (75), Expect = 0.044
Identities = 17/31 (54%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
Frame = +3
Query: 23 LMFWEPVLDRIKKRL-SRCSKFLSFGGRMVL 52
L WEP+L+ + K L S +K LSFGGR+VL
Sbjct: 228 LPMWEPLLEHVNKMLKSWGNKLLSFGGRIVL 320
>TC90620 similar to GP|14334592|gb|AAK59474.1 unknown protein {Arabidopsis
thaliana}, partial (40%)
Length = 813
Score = 28.9 bits (63), Expect = 1.1
Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 7/61 (11%)
Frame = +3
Query: 29 VLDRIKKRLSRCSKFLSFGGRMVLFKFVLSFIHV-------YALSFFKVPSGIISSMKSI 81
VL ++ LSR +++ G ++ F+L F HV Y LS+ V SG + + S
Sbjct: 588 VLIHLQDHLSRLKQWILLGLALLTLGFILHFSHVIPLNKQLYTLSYVCVTSGAAALVFSA 767
Query: 82 F 82
F
Sbjct: 768 F 770
>BF649019 similar to PIR|T49065|T49 hypothetical protein T5P19.290 -
Arabidopsis thaliana, partial (13%)
Length = 483
Score = 28.1 bits (61), Expect = 1.9
Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 14/92 (15%)
Frame = +1
Query: 3 QMGKVIFLYLGLPI-----GGDLSRLMFWEPVLDRIKKRLSRCSKF---------LSFGG 48
QMG ++ L LG+P+ G +R + W L + K +L RC++ ++F G
Sbjct: 109 QMGMMLVLLLGMPLKWVVRRGFFAR*VLW---LRKRKLKLRRCARLITRSSSLQSMNFAG 279
Query: 49 RMVLFKFVLSFIHVYALSFFKVPSGIISSMKS 80
+ K V LS+ ++ +SS+KS
Sbjct: 280 CCLTLKS*RVSSMVTILSYSRLAVTFLSSLKS 375
>TC83624 homologue to PIR|G84581|G84581 copia-like retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(1%)
Length = 831
Score = 28.1 bits (61), Expect = 1.9
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +1
Query: 4 MGKVIFLYLGLPIGGDLSRLMFWEPVLDRIK 34
+ +V F +LGLPIG + R +PVLD ++
Sbjct: 334 VNEVPFCFLGLPIGANPKRSSTRKPVLDSLQ 426
>TC91288 similar to PIR|T51354|T51354 cyclic nucleotide-regulated ion
channel 1 [validated] - Arabidopsis thaliana, partial
(22%)
Length = 841
Score = 27.3 bits (59), Expect = 3.2
Identities = 19/67 (28%), Positives = 30/67 (44%)
Frame = +2
Query: 16 IGGDLSRLMFWEPVLDRIKKRLSRCSKFLSFGGRMVLFKFVLSFIHVYALSFFKVPSGII 75
I + L F+ PV+D KK LSR K + + F + IH+ F+ +G I
Sbjct: 542 IAVSIDPLFFYIPVIDDGKKCLSRDKKMETTATVLRSFSDIFYIIHI----IFQFRTGFI 709
Query: 76 SSMKSIF 82
+ +F
Sbjct: 710 APSSRVF 730
>BF641241 homologue to GP|14279070|dbj tRNA isopentenyltransferase
{Arabidopsis thaliana}, partial (10%)
Length = 663
Score = 26.2 bits (56), Expect = 7.1
Identities = 22/55 (40%), Positives = 30/55 (54%), Gaps = 4/55 (7%)
Frame = +3
Query: 37 LSRCSKFLSFGGRMVLFKFVLSFIHVYAL---SFFKVPSGIISSMKSI-FIKKKK 87
LS S F + R + F VLS I +YA+ F + P IS++ I F+KKKK
Sbjct: 54 LSSRSNFETTKVRKLCFDSVLSLISIYAIVVCMFLRFP---ISNLDYISFLKKKK 209
>TC80325 homologue to PIR|F84674|F84674 probable AAA-type ATPase [imported]
- Arabidopsis thaliana, partial (23%)
Length = 723
Score = 25.8 bits (55), Expect = 9.2
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 5/60 (8%)
Frame = +2
Query: 12 LGLPIGGDLSRLMFWEPVLD----RIKKRLSRCSKFLSF-GGRMVLFKFVLSFIHVYALS 66
+G IG SR +F+ P+ +SR S F S + +F+F++S H YA++
Sbjct: 200 VGT*IGNGTSRNLFFIPLFPIRSPPSSSPISRVSLFPSHTNSQFSIFQFLISHTHTYAIT 379
>BI270951 homologue to GP|20906098|gb Cobalamin biosynthesis protein
{Methanosarcina mazei Goe1}, partial (0%)
Length = 432
Score = 25.8 bits (55), Expect = 9.2
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 11 YLGLPIGGDLSRLMFWEPVLDRIKKRLSRCSKFLSFGG 48
++G +G L L++W+ R+ L CS F +F G
Sbjct: 150 FIGFNLGKIL--LLYWQYWCSRVFSLLQHCSTFTTFSG 257
>TC90743 GP|16025115|gb|AAL11411.1 major histocompatibility class I receptor
{Stizostedion vitreum}, partial (2%)
Length = 685
Score = 25.8 bits (55), Expect = 9.2
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -1
Query: 57 LSFIHVYALSFFKVPSGIISSMKSIFIKKKKKK 89
LSF + S P+ S+ KS++I KKKKK
Sbjct: 472 LSFFRILYSSHTLTPTFFHSNFKSLWI*KKKKK 374
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.357 0.167 0.590
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,187,037
Number of Sequences: 36976
Number of extensions: 65443
Number of successful extensions: 1117
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1115
length of query: 175
length of database: 9,014,727
effective HSP length: 90
effective length of query: 85
effective length of database: 5,686,887
effective search space: 483385395
effective search space used: 483385395
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC142143.6