Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC142142.2 + phase: 0 
         (119 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC76737 homologue to SP|P06379|RK2_TOBAC Chloroplast 50S ribosom...   241  4e-65
CA859891 weakly similar to PIR|S09286|R5EG ribosomal protein L16...    44  1e-05
TC83827 similar to GP|21592923|gb|AAM64873.1 induced upon woundi...    29  0.23
BQ751763 similar to GP|18894036|gb| hypothetical protein {Pyroco...    26  2.5
TC79326 homologue to GP|14190381|gb|AAK55671.1 AT5g11200/F2I11_9...    25  4.3
TC87539 similar to PIR|T06308|T06308 protein phosphatase 2C homo...    25  4.3
CB065283 similar to PIR|F83356|F83 hypothetical protein PA2310 [...    25  5.6
TC88771 similar to GP|3643603|gb|AAC42250.1| unknown protein {Ar...    25  5.6
TC79831 similar to GP|10177116|dbj|BAB10406. contains similarity...    24  7.3
BG583596 similar to GP|4325376|gb| T3H13.12 gene product {Arabid...    24  7.3
TC83159                                                                24  9.6
TC81293 similar to GP|19911581|dbj|BAB86894. syringolide-induced...    24  9.6
TC85581 weakly similar to PIR|T14329|T14329 dermal glycoprotein ...    24  9.6
TC77791 similar to GP|10176862|dbj|BAB10068. emb|CAB80929.1~gene...    24  9.6

>TC76737 homologue to SP|P06379|RK2_TOBAC Chloroplast 50S ribosomal protein
            L2. [Common tobacco] {Nicotiana tabacum}, partial (95%)
          Length = 5466

 Score =  241 bits (614), Expect = 4e-65
 Identities = 119/119 (100%), Positives = 119/119 (100%)
 Frame = +1

Query: 1    MKGKAYRGNTISFGKYALQALEPAWITSRQIEAGRRAMSRNVRRGGKIWVRIFPDKPVTV 60
            MKGKAYRGNTISFGKYALQALEPAWITSRQIEAGRRAMSRNVRRGGKIWVRIFPDKPVTV
Sbjct: 4249 MKGKAYRGNTISFGKYALQALEPAWITSRQIEAGRRAMSRNVRRGGKIWVRIFPDKPVTV 4428

Query: 61   RPTETRMGSGKGSPEYWVAVVKPGKILYEMGGVPENIARKAISIAASKMPIRTQFILSE 119
            RPTETRMGSGKGSPEYWVAVVKPGKILYEMGGVPENIARKAISIAASKMPIRTQFILSE
Sbjct: 4429 RPTETRMGSGKGSPEYWVAVVKPGKILYEMGGVPENIARKAISIAASKMPIRTQFILSE 4605


>CA859891 weakly similar to PIR|S09286|R5EG ribosomal protein L16 - Euglena
           gracilis chloroplast, partial (33%)
          Length = 407

 Score = 43.5 bits (101), Expect = 1e-05
 Identities = 23/44 (52%), Positives = 30/44 (67%)
 Frame = +2

Query: 67  MGSGKGSPEYWVAVVKPGKILYEMGGVPENIARKAISIAASKMP 110
           MGSGKGS E  VAVVK   I+YE+ GV +++A K +  A  K+P
Sbjct: 2   MGSGKGSIEE*VAVVKS*TIVYEIKGVSKSVAYKVLKKAGDKLP 133


>TC83827 similar to GP|21592923|gb|AAM64873.1 induced upon wounding stress
           {Arabidopsis thaliana}, partial (38%)
          Length = 633

 Score = 29.3 bits (64), Expect = 0.23
 Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 8/77 (10%)
 Frame = -2

Query: 11  ISFGKYALQALEPAWITSRQIEAGRRA------MSRNVRRGGKIWVRIFPDKPVT--VRP 62
           +SFG      +     ++ Q   GRRA      +   V   G+I  R+FP  PVT  + P
Sbjct: 452 VSFGSVMSHWMYSIG*SAFQEGRGRRATP*TFHLPPGVSARGRISARLFPTMPVTPMISP 273

Query: 63  TETRMGSGKGSPEYWVA 79
           T  ++G    S  +++A
Sbjct: 272 TLWKVGGASSSSNFFLA 222


>BQ751763 similar to GP|18894036|gb| hypothetical protein {Pyrococcus
           furiosus DSM 3638}, partial (5%)
          Length = 712

 Score = 25.8 bits (55), Expect = 2.5
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = +2

Query: 22  EPAWITSRQIEAGRRAMSRNVRRGGKIWVR 51
           +P  I+      GRR  +R +RR G  WVR
Sbjct: 386 KPCTISRYTTVLGRRLAARTIRRTGGRWVR 475


>TC79326 homologue to GP|14190381|gb|AAK55671.1 AT5g11200/F2I11_90
           {Arabidopsis thaliana}, partial (94%)
          Length = 1883

 Score = 25.0 bits (53), Expect = 4.3
 Identities = 11/30 (36%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
 Frame = -3

Query: 47  KIWVR---IFPDKPVTVRPTETRMGSGKGS 73
           ++W+     FPD P+T+ PTE+   S   S
Sbjct: 237 ELWIPT*PFFPDSPLTLAPTESGAFSSSSS 148


>TC87539 similar to PIR|T06308|T06308 protein phosphatase 2C homolog
           F11C18.60 - Arabidopsis thaliana, partial (92%)
          Length = 1423

 Score = 25.0 bits (53), Expect = 4.3
 Identities = 12/16 (75%), Positives = 12/16 (75%)
 Frame = -3

Query: 59  TVRPTETRMGSGKGSP 74
           TVRPTE RMGS   SP
Sbjct: 590 TVRPTEIRMGSLLKSP 543


>CB065283 similar to PIR|F83356|F83 hypothetical protein PA2310 [imported] -
           Pseudomonas aeruginosa (strain PAO1), partial (37%)
          Length = 537

 Score = 24.6 bits (52), Expect = 5.6
 Identities = 13/29 (44%), Positives = 17/29 (57%), Gaps = 1/29 (3%)
 Frame = -3

Query: 25  WITSRQIEA-GRRAMSRNVRRGGKIWVRI 52
           W+T  Q E   RRA  RN R G  +WV++
Sbjct: 127 WLTPTQCETFNRRATIRNAR-GFPVWVQV 44


>TC88771 similar to GP|3643603|gb|AAC42250.1| unknown protein {Arabidopsis
           thaliana}, partial (33%)
          Length = 1295

 Score = 24.6 bits (52), Expect = 5.6
 Identities = 10/28 (35%), Positives = 15/28 (52%)
 Frame = +2

Query: 60  VRPTETRMGSGKGSPEYWVAVVKPGKIL 87
           + PT  R G G+     W+   KPG+I+
Sbjct: 797 INPTLLRSGCGRLFMLLWIGPSKPGRIM 880


>TC79831 similar to GP|10177116|dbj|BAB10406. contains similarity to
           ribonucleoprotein F~gene_id:K2A18.8 {Arabidopsis
           thaliana}, partial (85%)
          Length = 1184

 Score = 24.3 bits (51), Expect = 7.3
 Identities = 13/48 (27%), Positives = 23/48 (47%)
 Frame = +3

Query: 19  QALEPAWITSRQIEAGRRAMSRNVRRGGKIWVRIFPDKPVTVRPTETR 66
           +A   A++     +  +RAM ++    G  +V +FP  P   R  E+R
Sbjct: 831 KATGEAYVEFVSPDEAKRAMFKDKMTIGSRYVELFPSTPDEARRAESR 974


>BG583596 similar to GP|4325376|gb| T3H13.12 gene product {Arabidopsis
           thaliana}, partial (46%)
          Length = 778

 Score = 24.3 bits (51), Expect = 7.3
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +3

Query: 94  PENIARKAISIAASKMPIRTQFIL 117
           PEN    ++S+AA K+P ++  IL
Sbjct: 240 PENSTANSVSLAAGKLPNQSSNIL 311


>TC83159 
          Length = 371

 Score = 23.9 bits (50), Expect = 9.6
 Identities = 9/36 (25%), Positives = 18/36 (50%)
 Frame = +1

Query: 83  PGKILYEMGGVPENIARKAISIAASKMPIRTQFILS 118
           PGK+ Y  GGV  N+    +   +  +  +  ++L+
Sbjct: 190 PGKVYYVQGGVARNVXXMHVKARSKXIHDQCAWVLT 297


>TC81293 similar to GP|19911581|dbj|BAB86894. syringolide-induced protein
           B13-1-9 {Glycine max}, partial (60%)
          Length = 799

 Score = 23.9 bits (50), Expect = 9.6
 Identities = 13/40 (32%), Positives = 18/40 (44%)
 Frame = -3

Query: 9   NTISFGKYALQALEPAWITSRQIEAGRRAMSRNVRRGGKI 48
           N +SF    L      W TS++I   R A +   RR  +I
Sbjct: 227 NLVSFASLTLNLKVRGWTTSQKIRIMRPARTMIARRSFQI 108


>TC85581 weakly similar to PIR|T14329|T14329 dermal glycoprotein precursor
           extracellular - carrot (fragment), partial (72%)
          Length = 1570

 Score = 23.9 bits (50), Expect = 9.6
 Identities = 13/50 (26%), Positives = 22/50 (44%), Gaps = 7/50 (14%)
 Frame = -3

Query: 48  IWVRIFPDKPVTVRPTETRMGSGKGSP-------EYWVAVVKPGKILYEM 90
           +WV +FP   + V   +   G  K +P       E+W    + G+ + EM
Sbjct: 419 LWVTLFPGATLQVLLLQPGFGDEKQTPQSSNLAREHWAERHRAGRYVEEM 270


>TC77791 similar to GP|10176862|dbj|BAB10068.
           emb|CAB80929.1~gene_id:MRO11.1~similar to unknown
           protein {Arabidopsis thaliana}, partial (31%)
          Length = 1053

 Score = 23.9 bits (50), Expect = 9.6
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -2

Query: 44  RGGKIWVRIFPDKPVTVRPTETRMGSGKGSP 74
           RGG + +  FP   +T  P E   G+G G+P
Sbjct: 629 RGGGVVLYGFPYVTLTPPPYEFPYGAGAGAP 537


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.319    0.135    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,162,940
Number of Sequences: 36976
Number of extensions: 36219
Number of successful extensions: 147
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of query: 119
length of database: 9,014,727
effective HSP length: 95
effective length of query: 24
effective length of database: 5,502,007
effective search space: 132048168
effective search space used: 132048168
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)


Medicago: description of AC142142.2