BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141414.1 - phase: 0
(104 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89108 150 7e-38
TC80000 weakly similar to GP|15451136|gb|AAK96839.1 periaxin-lik... 28 0.43
TC85527 homologue to GP|13489167|gb|AAK27801.1 60S ribosomal pro... 28 0.43
TC91432 homologue to GP|14532556|gb|AAK64006.1 AT5g57360/MSF19_2... 27 0.95
TC80001 weakly similar to GP|15451136|gb|AAK96839.1 periaxin-lik... 27 1.2
AJ500206 27 1.2
TC93254 weakly similar to GP|10176972|dbj|BAB10190. contains sim... 26 2.1
AW695258 similar to PIR|E71438|E714 probable transcription facto... 26 2.8
TC86720 similar to PIR|T47941|T47941 hypothetical protein F2A19.... 26 2.8
CB892264 weakly similar to GP|17131851|dbj serine proteinase {No... 26 2.8
TC78713 similar to GP|21310093|gb|AAM46142.1 terminal flower-lik... 25 3.6
BQ135608 similar to GP|4325337|gb|A F15P23.3 gene product {Arabi... 25 4.7
TC93461 25 6.2
TC84558 weakly similar to SP|Q04634|EF1A_TETPY Elongation factor... 25 6.2
CA860319 weakly similar to PIR|T38663|T386 probable translation ... 25 6.2
TC89368 similar to SP|O22160|TL15_ARATH Thylakoid lumenal 15 kDa... 25 6.2
TC88526 similar to PIR|T49291|T49291 hypothetical protein T16L24... 25 6.2
BE326156 similar to GP|9759609|dbj gene_id:MNA5.1~unknown protei... 24 8.1
BG646771 similar to GP|11994562|db emb|CAB68186.1~gene_id:K20M4.... 24 8.1
BE204888 similar to GP|21595622|gb| unknown {Arabidopsis thalian... 24 8.1
>TC89108
Length = 673
Score = 150 bits (379), Expect = 7e-38
Identities = 68/70 (97%), Positives = 69/70 (98%)
Frame = +1
Query: 25 VMSSEKEELSSTVKRKLSCFEHFDALWFCYSPVYQMKQYYRLGTLDNCRGKWKAWTDCLM 84
VMSSEKEE SSTVKRKLSCFEHFDALWFCYSPVYQMKQYYRLGTLDNCRGKWKAWTDCLM
Sbjct: 16 VMSSEKEESSSTVKRKLSCFEHFDALWFCYSPVYQMKQYYRLGTLDNCRGKWKAWTDCLM 195
Query: 85 LKTKPKSQVE 94
LKTKPKSQ+E
Sbjct: 196LKTKPKSQLE 225
>TC80000 weakly similar to GP|15451136|gb|AAK96839.1 periaxin-like protein
{Arabidopsis thaliana}, partial (55%)
Length = 1033
Score = 28.5 bits (62), Expect = 0.43
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = -2
Query: 6 MTGFFSKEFIGCSNFQFVAVMSSEKEELSSTVKRKLSCF 44
++GF + +GCSNF ++ S + S T+ SCF
Sbjct: 606 ISGFVNSGTLGCSNFGDSGILGSSGFDNSGTLNLGSSCF 490
>TC85527 homologue to GP|13489167|gb|AAK27801.1 60S ribosomal protein L21
{Oryza sativa}, partial (34%)
Length = 470
Score = 28.5 bits (62), Expect = 0.43
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +1
Query: 64 YRLGTLDNCRGKWKAWTDCLMLKTKPKSQV 93
YRLG + C WK D L + K K +V
Sbjct: 94 YRLGRREECESAWKIKNDQLKAEAKAKGEV 183
>TC91432 homologue to GP|14532556|gb|AAK64006.1 AT5g57360/MSF19_2
{Arabidopsis thaliana}, partial (21%)
Length = 755
Score = 27.3 bits (59), Expect = 0.95
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +1
Query: 17 CSNFQFVAVMSSEKEELSSTVKRKLSCFEHFDALW 51
C NF + + SS +L + VK L HF+ +W
Sbjct: 367 CMNFHWQILSSSPVNKLYAAVKGMLML*NHFEQIW 471
>TC80001 weakly similar to GP|15451136|gb|AAK96839.1 periaxin-like protein
{Arabidopsis thaliana}, partial (38%)
Length = 532
Score = 26.9 bits (58), Expect = 1.2
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = -1
Query: 6 MTGFFSKEFIGCSNFQFVAVMSSEKEELSSTVKRKLSCF 44
++GF + +GCSNF ++ S S T+ SCF
Sbjct: 493 ISGFVNSGTLGCSNFGDSGILGSSGFGNSGTLNLGSSCF 377
>AJ500206
Length = 286
Score = 26.9 bits (58), Expect = 1.2
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +2
Query: 1 MVIYDMTGFFSKEFIGCSNFQFVA 24
++I D GF+ + IGCSNF FVA
Sbjct: 119 LIIPDRRGFWKR--IGCSNFFFVA 184
>TC93254 weakly similar to GP|10176972|dbj|BAB10190. contains similarity to
DNA repair protein~gene_id:MTG13.7 {Arabidopsis
thaliana}, partial (8%)
Length = 647
Score = 26.2 bits (56), Expect = 2.1
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = -3
Query: 17 CSNFQFVAVMSSEKEELSSTVKRKLSCFEHFDALWFCYSPVYQMKQYYR 65
CSN F V S + + S LSC F A++FC P+ + ++R
Sbjct: 600 CSNHGFNLVFSINNQ-IHSIHMHPLSCQIFFTAIYFC--PIQRRSYFHR 463
>AW695258 similar to PIR|E71438|E714 probable transcription factor d14540c -
Arabidopsis thaliana, partial (39%)
Length = 637
Score = 25.8 bits (55), Expect = 2.8
Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +3
Query: 17 CSNFQFVAVMSSEKEELSSTVKRKLSCFEHFDALWFCYSPVYQMK---QYYRLGTLDNCR 73
CS FQ++ + + E+ S VK F F + + S V + K + RLG+L R
Sbjct: 303 CSQFQYLEIAPNINLEVDSLVK----FFVKFHSFFLFPSFV*KSKTKSKCLRLGSLP--R 464
Query: 74 GKWKAWTDCL 83
W W CL
Sbjct: 465 ISWTWWLQCL 494
>TC86720 similar to PIR|T47941|T47941 hypothetical protein F2A19.90 -
Arabidopsis thaliana, partial (85%)
Length = 1719
Score = 25.8 bits (55), Expect = 2.8
Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = +1
Query: 73 RGKWK-AWTDCLMLKTKPKSQVEVGS 97
RGKW W+ C+++ T + +VEV S
Sbjct: 601 RGKWHHRWSGCILVATISQKKVEVHS 678
>CB892264 weakly similar to GP|17131851|dbj serine proteinase {Nostoc sp. PCC
7120}, partial (16%)
Length = 830
Score = 25.8 bits (55), Expect = 2.8
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 8/40 (20%)
Frame = +2
Query: 42 SCFEHFDA---LWFCYSPVYQM-----KQYYRLGTLDNCR 73
SC +FD+ LWFCY Y + +++Y L CR
Sbjct: 431 SCC*YFDSTRFLWFCYREEYWLWNNH*QRWYNLDMCSCCR 550
>TC78713 similar to GP|21310093|gb|AAM46142.1 terminal flower-like protein 1
{Vitis vinifera}, partial (95%)
Length = 931
Score = 25.4 bits (54), Expect = 3.6
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = -1
Query: 29 EKEELSSTVKRKLSCFEHFDALW 51
E + LSS V R+ SC H LW
Sbjct: 283 EHQGLSSPVYRRTSCHHHQSQLW 215
>BQ135608 similar to GP|4325337|gb|A F15P23.3 gene product {Arabidopsis
thaliana}, partial (4%)
Length = 759
Score = 25.0 bits (53), Expect = 4.7
Identities = 11/31 (35%), Positives = 15/31 (47%), Gaps = 5/31 (16%)
Frame = +1
Query: 51 WFCYSPVYQ-----MKQYYRLGTLDNCRGKW 76
WF YSP YQ + Y+ + + CR W
Sbjct: 238 WFAYSPAYQE*PHYSRLYF*ILLVRECRVTW 330
>TC93461
Length = 612
Score = 24.6 bits (52), Expect = 6.2
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = -1
Query: 82 CLMLKTKPKSQVEVGSYFL 100
C+ML PKS++E G++ L
Sbjct: 516 CIMLFLSPKSELEPGNFLL 460
>TC84558 weakly similar to SP|Q04634|EF1A_TETPY Elongation factor 1-alpha
(EF-1-alpha) (14-nm filament-associated protein).
{Tetrahymena pyriformis}, partial (42%)
Length = 611
Score = 24.6 bits (52), Expect = 6.2
Identities = 11/29 (37%), Positives = 12/29 (40%)
Frame = +1
Query: 50 LWFCYSPVYQMKQYYRLGTLDNCRGKWKA 78
LW P Q + Y G NC WKA
Sbjct: 1 LWCRNRPSRQSRDRYHQGRYANCLHSWKA 87
>CA860319 weakly similar to PIR|T38663|T386 probable translation initiation
factor eif-2b beta subunit - fission yeast, partial
(22%)
Length = 767
Score = 24.6 bits (52), Expect = 6.2
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +3
Query: 51 WFCYSPVYQMKQYYRLGTLDNCRGKWKAWTDCLMLKTK 88
W+ ++P QM++ L ++ WK +M+K+K
Sbjct: 273 WYLHNPELQMEELMELVVSNHFHK*WKMKKRMMMMKSK 386
>TC89368 similar to SP|O22160|TL15_ARATH Thylakoid lumenal 15 kDa protein
chloroplast precursor (p15). [Mouse-ear cress], partial
(75%)
Length = 767
Score = 24.6 bits (52), Expect = 6.2
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -1
Query: 28 SEKEELSSTVKRKLSCFEHFDALW 51
+EKEEL S +KRK+ D +W
Sbjct: 131 TEKEELVSWLKRKVLTLRDGDEVW 60
>TC88526 similar to PIR|T49291|T49291 hypothetical protein T16L24.50 -
Arabidopsis thaliana, partial (96%)
Length = 1064
Score = 24.6 bits (52), Expect = 6.2
Identities = 21/74 (28%), Positives = 30/74 (40%), Gaps = 20/74 (27%)
Frame = +2
Query: 23 VAVMSSEKEELSSTVK--RKLSCFEHF---------DALWFCYSPVYQMKQYYRL----- 66
V + EK++ +ST R+LSC EHF D W C + +Q + L
Sbjct: 5 VPIRHEEKKKKTSTRCGFRRLSCIEHFTQ*ECITMSDHNWGCLNFSLNFQQAHNLMCLGM 184
Query: 67 ----GTLDNCRGKW 76
LD+ G W
Sbjct: 185HLMQPVLDSFEGDW 226
>BE326156 similar to GP|9759609|dbj gene_id:MNA5.1~unknown protein
{Arabidopsis thaliana}, partial (22%)
Length = 522
Score = 24.3 bits (51), Expect = 8.1
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +2
Query: 66 LGTLDNCRGKWKAWT 80
LG L NCR W WT
Sbjct: 350 LGLLSNCRIHWLFWT 394
>BG646771 similar to GP|11994562|db emb|CAB68186.1~gene_id:K20M4.4~strong
similarity to unknown protein {Arabidopsis thaliana},
partial (23%)
Length = 663
Score = 24.3 bits (51), Expect = 8.1
Identities = 11/32 (34%), Positives = 16/32 (49%)
Frame = +1
Query: 71 NCRGKWKAWTDCLMLKTKPKSQVEVGSYFLLI 102
N G+WK W L +T+ S S+F L+
Sbjct: 88 NILGRWKGWRMKLSSRTESSSTSIQESFFTLL 183
>BE204888 similar to GP|21595622|gb| unknown {Arabidopsis thaliana}, partial
(16%)
Length = 632
Score = 24.3 bits (51), Expect = 8.1
Identities = 17/52 (32%), Positives = 22/52 (41%), Gaps = 10/52 (19%)
Frame = +2
Query: 41 LSCFEHFDALWFCYSPVYQMKQYYRLGTL----------DNCRGKWKAWTDC 82
++C + F L FC + V MK +L TL C G W W DC
Sbjct: 401 IACLKVF--LLFCLTKVICMKAT-KLTTLLYTRAVWACRTRCYGGWNRWIDC 547
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.137 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,378,904
Number of Sequences: 36976
Number of extensions: 68314
Number of successful extensions: 489
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 487
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 489
length of query: 104
length of database: 9,014,727
effective HSP length: 80
effective length of query: 24
effective length of database: 6,056,647
effective search space: 145359528
effective search space used: 145359528
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)
Medicago: description of AC141414.1