BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141107.11 + phase: 0 /pseudo
(650 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77245 similar to GP|6630556|gb|AAF19575.1| putative alpha-L-ar... 822 0.0
AW685281 weakly similar to GP|17380938|gb putative arabinosidase... 408 e-114
AJ388681 similar to GP|13398412|gb arabinoxylan arabinofuranohyd... 264 2e-83
BI264996 weakly similar to GP|16417958|gb arabinosidase ARA-1 {L... 223 2e-58
TC93206 similar to GP|13398412|gb|AAK21879.1 arabinoxylan arabin... 204 3e-54
BE248680 weakly similar to GP|13937191|gb AT3g10740/T7M13_18 {Ar... 127 2e-29
BQ751454 weakly similar to GP|23326899|gb| alpha-L-arabinosidase... 42 6e-04
TC87431 32 1.00
TC87430 32 1.00
BF646125 30 3.8
TC78514 homologue to SP|Q9SMJ3|ZDS_CAPAN Zeta-carotene desaturas... 30 3.8
>TC77245 similar to GP|6630556|gb|AAF19575.1| putative
alpha-L-arabinofuranosidase {Arabidopsis thaliana},
partial (89%)
Length = 2351
Score = 822 bits (2123), Expect = 0.0
Identities = 404/657 (61%), Positives = 499/657 (75%), Gaps = 8/657 (1%)
Frame = +2
Query: 1 MTFSKACCSFLWLYLIIVSFVAFQC---NANGSQISSLVVNAAQ--GRPMPNTLFGIFYE 55
M FSK C L L+IV ++ QC ++LVV+A+Q GR +P TLFGIF+E
Sbjct: 203 MGFSKDSCCVFMLQLLIVVYLVVQCFDVQVQADLNATLVVDASQASGRRIPETLFGIFFE 382
Query: 56 EINHAGSGGIWAQLVNNSGFEAAGTRTPSNIFPWTIIGTESSVKLQTELSSCFERNKVAL 115
EINHAG+GG+WA+LV+N GFEA G PSNI PW+IIG + + ++T+ +SCFERNKVAL
Sbjct: 383 EINHAGAGGLWAELVSNRGFEAGGPNIPSNIDPWSIIGNATYINVETDRTSCFERNKVAL 562
Query: 116 RMDVLCD-KCPPDGVGVSNPGFWGMNIVQGKKYKVVFFYRSLGSLDMRVAFRDAISGRIL 174
R++VLCD CP DGVGV NPGFWGMNI QGKKYKVVF+ RS G L+++V+ + L
Sbjct: 563 RLEVLCDGTCPTDGVGVYNPGFWGMNIEQGKKYKVVFYARSTGPLNLKVSLTGSNGVGSL 742
Query: 175 ASSHIIRHKASKKKGSKWQRVQTILEARASSSNSNLTLTTTKEGTVWLDQVSAMPTDTFK 234
AS+ I S S W +V+T+LEA+A++ NS L LTTT +G +WLDQVSAMP DT+K
Sbjct: 743 ASTVIT---GSASDFSNWTKVETVLEAKATNPNSRLQLTTTTKGVIWLDQVSAMPLDTYK 913
Query: 235 GHGFRKDLVEMLIQLKPAFLRFPGGCFVEGVQLRNAFRWKDSVGPWEQRPGHLNDIWNYW 294
GHGFR DL++ML+ LKP+F+RFPGGCFVEG LRNAFRWK +VGPWE+RPGH D+W YW
Sbjct: 914 GHGFRSDLLQMLVDLKPSFIRFPGGCFVEGDYLRNAFRWKAAVGPWEERPGHFGDVWKYW 1093
Query: 295 TDDGLGFFEGLQLAEDIGALPIWVFNNGISHSDEVDTSVISPFVKEALEGIEFARGSSTS 354
TDDGLG++E LQL+ED+GALPIWVFNNG+SH+DEVDTS + PFV+EAL+G+EFARG TS
Sbjct: 1094TDDGLGYYEFLQLSEDLGALPIWVFNNGVSHNDEVDTSAVLPFVQEALDGLEFARGDPTS 1273
Query: 355 KWGSVRASMGHPKPFNLKYVAIGNEDCYKKNYYGNYMAFYKAIKKFYPDIQIISNCPAFK 414
KWGS+RA+MGHP+PFNLKYVA+GNEDC KKNY GNY+ FY AI++ YPDIQIISNC
Sbjct: 1274KWGSMRAAMGHPEPFNLKYVAVGNEDCGKKNYRGNYLRFYDAIRRAYPDIQIISNCDGSS 1453
Query: 415 TPLNHPADLYDYHTYPIDARAMFNAYHDFDKSPRNGPKAFVSEYALIGALQAKYGTLLGA 474
PL+HPAD+YDYH Y +A MF+ F++ R+GPKAFVSEYA+ G A G+LL A
Sbjct: 1454RPLDHPADMYDYHIY-TNANDMFSRSTTFNRVTRSGPKAFVSEYAVTGN-DAGQGSLLAA 1627
Query: 475 VSEAGFLIGLERNSDHVAMASYAPLLVNANDRNWNPDAIVFNSYQAYGTPSYWVTYMFKE 534
++EAGFLIGLE+NSD V MASYAPL VNANDR WNPDAIVFNS+Q YGTPSYW+ F E
Sbjct: 1628LAEAGFLIGLEKNSDIVHMASYAPLFVNANDRRWNPDAIVFNSFQLYGTPSYWMQLFFSE 1807
Query: 535 SNGATFLNSQLQTPDPGSLIASAILCQSPQNNSTYLKIKIANIGSIPVNLKISLQGYVSK 594
SNGAT LNS LQT SL+ASAI Q+ + Y++IK N G+ VNLKIS G
Sbjct: 1808SNGATLLNSSLQTTASNSLVASAITWQNSVDKKNYIRIKAVNFGTSAVNLKISFNGLDPN 1987
Query: 595 NL--AGSTKTVLTSGNILDENTFAAPKKIAPQTSPLQNPGNEMNVMIPPVSLTVLDM 649
+L +GSTKTVLTS N++DEN+F+ PKK+ P S LQ+ G +MNV++PP S T D+
Sbjct: 1988SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDL 2158
>AW685281 weakly similar to GP|17380938|gb putative arabinosidase
{Arabidopsis thaliana}, partial (18%)
Length = 651
Score = 408 bits (1049), Expect = e-114
Identities = 202/209 (96%), Positives = 203/209 (96%)
Frame = +2
Query: 1 MTFSKACCSFLWLYLIIVSFVAFQCNANGSQISSLVVNAAQGRPMPNTLFGIFYEEINHA 60
MTFSKACCSFLWLYLIIVSFVAFQCNANGSQISSLVVNAAQGRPMPNTLFGIFYEEINHA
Sbjct: 23 MTFSKACCSFLWLYLIIVSFVAFQCNANGSQISSLVVNAAQGRPMPNTLFGIFYEEINHA 202
Query: 61 GSGGIWAQLVNNSGFEAAGTRTPSNIFPWTIIGTESSVKLQTELSSCFERNKVALRMDVL 120
GSGGIWAQLVNNSGFEAAGTRTPSNIFPWTIIGTESSVKLQTELSSCFERNKVALRMDVL
Sbjct: 203 GSGGIWAQLVNNSGFEAAGTRTPSNIFPWTIIGTESSVKLQTELSSCFERNKVALRMDVL 382
Query: 121 CDKCPPDGVGVSNPGFWGMNIVQGKKYKVVFFYRSLGSLDMRVAFRDAISGRILASSHII 180
CDKCPPDGVGVSNPGFWGMNIVQGKKYKVVFFYRSLGSLDMRVAFRDAI GRILASSHII
Sbjct: 383 CDKCPPDGVGVSNPGFWGMNIVQGKKYKVVFFYRSLGSLDMRVAFRDAIXGRILASSHII 562
Query: 181 RHKASKKKGSKWQRVQTILEARASSSNSN 209
RHKASKK+ K Q VQTILEARASS NSN
Sbjct: 563 RHKASKKRVPKXQXVQTILEARASSXNSN 649
>AJ388681 similar to GP|13398412|gb arabinoxylan arabinofuranohydrolase
isoenzyme AXAH-I {Hordeum vulgare}, partial (18%)
Length = 574
Score = 264 bits (675), Expect(2) = 2e-83
Identities = 125/125 (100%), Positives = 125/125 (100%)
Frame = +1
Query: 297 DGLGFFEGLQLAEDIGALPIWVFNNGISHSDEVDTSVISPFVKEALEGIEFARGSSTSKW 356
DGLGFFEGLQLAEDIGALPIWVFNNGISHSDEVDTSVISPFVKEALEGIEFARGSSTSKW
Sbjct: 1 DGLGFFEGLQLAEDIGALPIWVFNNGISHSDEVDTSVISPFVKEALEGIEFARGSSTSKW 180
Query: 357 GSVRASMGHPKPFNLKYVAIGNEDCYKKNYYGNYMAFYKAIKKFYPDIQIISNCPAFKTP 416
GSVRASMGHPKPFNLKYVAIGNEDCYKKNYYGNYMAFYKAIKKFYPDIQIISNCPAFKTP
Sbjct: 181 GSVRASMGHPKPFNLKYVAIGNEDCYKKNYYGNYMAFYKAIKKFYPDIQIISNCPAFKTP 360
Query: 417 LNHPA 421
LNHPA
Sbjct: 361 LNHPA 375
Score = 64.3 bits (155), Expect(2) = 2e-83
Identities = 26/30 (86%), Positives = 28/30 (92%)
Frame = +2
Query: 422 DLYDYHTYPIDARAMFNAYHDFDKSPRNGP 451
DLYDYHTYPIDAR MFNAYH+FDKSP +GP
Sbjct: 377 DLYDYHTYPIDARXMFNAYHEFDKSPXHGP 466
>BI264996 weakly similar to GP|16417958|gb arabinosidase ARA-1 {Lycopersicon
esculentum}, partial (20%)
Length = 422
Score = 223 bits (568), Expect = 2e-58
Identities = 96/140 (68%), Positives = 116/140 (82%)
Frame = +2
Query: 229 PTDTFKGHGFRKDLVEMLIQLKPAFLRFPGGCFVEGVQLRNAFRWKDSVGPWEQRPGHLN 288
P DT+KGHGFR +L +M+ +LKP RFPGGC+VEG L+NAF+WK ++GPWE RPGH
Sbjct: 2 PLDTYKGHGFRMNLFQMVAELKPRXFRFPGGCYVEGNVLKNAFQWKQTIGPWENRPGHYG 181
Query: 289 DIWNYWTDDGLGFFEGLQLAEDIGALPIWVFNNGISHSDEVDTSVISPFVKEALEGIEFA 348
D+W+YWTDDG GFFEGLQLAED+ ALPIWVFNNGISHS++V+ S ISP V+ AL+GIE A
Sbjct: 182 DVWDYWTDDGFGFFEGLQLAEDLNALPIWVFNNGISHSEQVNVSAISPSVQXALDGIEXA 361
Query: 349 RGSSTSKWGSVRASMGHPKP 368
GS TS+WGS+ A MGHPKP
Sbjct: 362 IGSPTSRWGSIIAXMGHPKP 421
>TC93206 similar to GP|13398412|gb|AAK21879.1 arabinoxylan
arabinofuranohydrolase isoenzyme AXAH-I {Hordeum
vulgare}, partial (6%)
Length = 642
Score = 204 bits (520), Expect(2) = 3e-54
Identities = 101/133 (75%), Positives = 114/133 (84%)
Frame = +1
Query: 518 YQAYGTPSYWVTYMFKESNGATFLNSQLQTPDPGSLIASAILCQSPQNNSTYLKIKIANI 577
+Q YGTPSYWVTY+FKESNGATFLNS LQT DPG+L ASAIL + PQN +TYLKIKIAN+
Sbjct: 37 HQVYGTPSYWVTYLFKESNGATFLNSTLQTTDPGTLAASAILVKDPQNKNTYLKIKIANM 216
Query: 578 GSIPVNLKISLQGYVSKNLAGSTKTVLTSGNILDENTFAAPKKIAPQTSPLQNPGNEMNV 637
V+ KIS+QG+ SKNL GSTKTVLT GN LDEN+FA PKKIAPQTSPLQNPGNEMNV
Sbjct: 217 RKTQVDFKISIQGFASKNLKGSTKTVLT-GNELDENSFAEPKKIAPQTSPLQNPGNEMNV 393
Query: 638 MIPPVSLTVLDMF 650
++ P SLT+LDMF
Sbjct: 394 IVQPTSLTILDMF 432
Score = 26.2 bits (56), Expect(2) = 3e-54
Identities = 10/15 (66%), Positives = 12/15 (79%)
Frame = +2
Query: 508 WNPDAIVFNSYQAYG 522
WNPDAIVF+S + G
Sbjct: 8 WNPDAIVFSSIKFMG 52
>BE248680 weakly similar to GP|13937191|gb AT3g10740/T7M13_18 {Arabidopsis
thaliana}, partial (15%)
Length = 375
Score = 127 bits (318), Expect = 2e-29
Identities = 62/99 (62%), Positives = 78/99 (78%)
Frame = +1
Query: 479 GFLIGLERNSDHVAMASYAPLLVNANDRNWNPDAIVFNSYQAYGTPSYWVTYMFKESNGA 538
GFLIGLE+NSD V+M +YAPL VN NDR W PDAIVF+S+Q YG PSYW+ +FKES+GA
Sbjct: 76 GFLIGLEKNSDVVSMVNYAPLFVNTNDRKWTPDAIVFDSHQVYGIPSYWLIKLFKESSGA 255
Query: 539 TFLNSQLQTPDPGSLIASAILCQSPQNNSTYLKIKIANI 577
TFLNS LQT P +L ASAI +S + ++ L+IK+AN+
Sbjct: 256 TFLNSTLQTDSP-TLAASAISWKSSVDGTSILRIKVANL 369
>BQ751454 weakly similar to GP|23326899|gb| alpha-L-arabinosidase
{Bifidobacterium longum NCC2705}, partial (12%)
Length = 780
Score = 42.4 bits (98), Expect = 6e-04
Identities = 21/48 (43%), Positives = 28/48 (57%)
Frame = +3
Query: 237 GFRKDLVEMLIQLKPAFLRFPGGCFVEGVQLRNAFRWKDSVGPWEQRP 284
GFRKD+VE + +L +R+PGG F + W D VGP E+RP
Sbjct: 255 GFRKDVVEAVKELNCPVMRYPGGNFCA------TYHWIDGVGPREKRP 380
>TC87431
Length = 662
Score = 31.6 bits (70), Expect = 1.00
Identities = 13/21 (61%), Positives = 15/21 (70%)
Frame = +2
Query: 258 GGCFVEGVQLRNAFRWKDSVG 278
GGCFVE LRN +WK +VG
Sbjct: 437 GGCFVERNYLRNEIQWKAAVG 499
>TC87430
Length = 649
Score = 31.6 bits (70), Expect = 1.00
Identities = 13/21 (61%), Positives = 15/21 (70%)
Frame = +3
Query: 258 GGCFVEGVQLRNAFRWKDSVG 278
GGCFVE LRN +WK +VG
Sbjct: 459 GGCFVERNYLRNEIQWKAAVG 521
>BF646125
Length = 658
Score = 29.6 bits (65), Expect = 3.8
Identities = 21/65 (32%), Positives = 32/65 (48%)
Frame = +3
Query: 567 STYLKIKIANIGSIPVNLKISLQGYVSKNLAGSTKTVLTSGNILDENTFAAPKKIAPQTS 626
+T LK K +N S +N K + K+ K +L +L T +P K+ PQT+
Sbjct: 402 NTLLKPKSSN--STKINTKRNNHIQAHKHT*NIIKIILLKNGLLHSQT--SPTKLWPQTT 569
Query: 627 PLQNP 631
P +NP
Sbjct: 570 PKKNP 584
>TC78514 homologue to SP|Q9SMJ3|ZDS_CAPAN Zeta-carotene desaturase
chloroplast precursor (EC 1.14.99.30) (Carotene 7
8-desaturase)., partial (88%)
Length = 2063
Score = 29.6 bits (65), Expect = 3.8
Identities = 17/68 (25%), Positives = 28/68 (41%)
Frame = +3
Query: 355 KWGSVRASMGHPKPFNLKYVAIGNEDCYKKNYYGNYMAFYKAIKKFYPDIQIISNCPAFK 414
K+ + S H P L + I Y K N ++F++ + YP + + +CPA
Sbjct: 33 KFSNPSISSQHT*PDTLLFHLIHKNISYNKTIVLNSLSFFQLSRFSYPMMTSLIHCPATS 212
Query: 415 TPLNHPAD 422
P D
Sbjct: 213 LSTTRPGD 236
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,645,186
Number of Sequences: 36976
Number of extensions: 315673
Number of successful extensions: 1488
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1473
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1479
length of query: 650
length of database: 9,014,727
effective HSP length: 102
effective length of query: 548
effective length of database: 5,243,175
effective search space: 2873259900
effective search space used: 2873259900
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC141107.11