BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140916.4 - phase: 0
(250 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77458 similar to PIR|F96556|F96556 IAA-Ala hydrolase (IAR3) [i... 254 3e-68
TC84322 weakly similar to GP|3036871|emb|CAA06486.1 IAA amidohyd... 251 2e-67
AW980900 weakly similar to GP|18389266|gb IAA-amino acid hydrola... 249 5e-67
TC77457 similar to PIR|F96556|F96556 IAA-Ala hydrolase (IAR3) [i... 247 3e-66
TC87843 weakly similar to GP|3420801|gb|AAC31939.1| IAA-amino ac... 231 2e-61
TC80526 weakly similar to GP|18844936|dbj|BAB85405. putative IAA... 125 6e-61
TC89442 similar to GP|17978838|gb|AAL47552.1 IAA-amino acid conj... 147 3e-36
BE123972 similar to PIR|F96556|F96 IAA-Ala hydrolase (IAR3) [imp... 96 3e-30
BE239385 similar to GP|18844936|dbj putative IAA-Ala hydrolase {... 120 5e-28
TC79815 similar to GP|2181184|emb|CAA73905.1 JR3 protein {Arabid... 72 3e-13
BG587186 similar to GP|18844936|dbj putative IAA-Ala hydrolase {... 58 3e-09
BF645682 similar to GP|7270509|emb| NAM / CUC2-like protein {Ara... 45 3e-05
AW736524 similar to GP|2181184|emb JR3 protein {Arabidopsis thal... 42 2e-04
TC91251 similar to PIR|T00553|T00553 disease resistance response... 31 0.51
TC77091 weakly similar to GP|11602751|emb|CAC18558. ENOD18 prote... 29 1.9
TC77570 similar to PIR|H96751|H96751 probable casein kinase I F2... 28 4.3
>TC77458 similar to PIR|F96556|F96556 IAA-Ala hydrolase (IAR3) [imported] -
Arabidopsis thaliana, partial (85%)
Length = 1574
Score = 254 bits (648), Expect = 3e-68
Identities = 124/246 (50%), Positives = 172/246 (69%)
Frame = +3
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
++ GALE V AIF +HV + P G + SR GP+ AG GFF+AVISG+ AA P++S D
Sbjct: 603 ILDAGALEKVSAIFGLHVLNNLPLGEVASRSGPIFAGNGFFKAVISGRGGHAAIPQHSID 782
Query: 61 PVLAASAAVISIQGIVSRESNPLDSQVVSVTSFNGGNSHDMIPDSVVIGGTFRAFSNTSF 120
P+LA S ++S+Q IVSRE +PLDSQV++V GG + ++IPDSV IGGTFRAFSN SF
Sbjct: 783 PILATSNVIVSLQQIVSREIDPLDSQVLTVAMIQGGGAFNVIPDSVTIGGTFRAFSNESF 962
Query: 121 YQLLERIEQVIVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNF 180
QL RIEQ+I QA+V C A V F E+E +PPTVND ++++ + V+ LLG
Sbjct: 963 TQLRHRIEQIITGQAAVQRCHATVSFLEEEKPFFPPTVNDGGLHDYFQSVAGSLLGADKV 1142
Query: 181 RVVPPMMGAEDYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHAT 240
+ + PMMG+ED++FY + IP F +G+ + ++ +GHSP+F ++ED LP GAA+HA+
Sbjct: 1143KGMQPMMGSEDFAFYQEAIPGYIFLLGMEDVSVERLPSGHSPYFKVNEDVLPYGAALHAS 1322
Query: 241 IAERYL 246
+A RYL
Sbjct: 1323LASRYL 1340
>TC84322 weakly similar to GP|3036871|emb|CAA06486.1 IAA amidohydrolase
{Linum usitatissimum}, partial (92%)
Length = 866
Score = 251 bits (641), Expect = 2e-67
Identities = 124/245 (50%), Positives = 170/245 (68%)
Frame = +2
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
MIQ+ LEDV AIF ++ +P G++ SRPG LAG G F+A I G A P+ D
Sbjct: 17 MIQENVLEDVRAIFGCILASLYPLGVVASRPGEFLAGYGSFKAKIKG--G*AGTPQRCLD 190
Query: 61 PVLAASAAVISIQGIVSRESNPLDSQVVSVTSFNGGNSHDMIPDSVVIGGTFRAFSNTSF 120
P+LAASA+VIS+Q I+SRE +PLDSQV+SV + H++ PDSV GGT+RAFS SF
Sbjct: 191 PILAASASVISLQNIISREVDPLDSQVLSVAMIQSESGHELTPDSVTFGGTYRAFSKKSF 370
Query: 121 YQLLERIEQVIVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNF 180
L RIE+VI QA VY C AEV+FF +E+ PPT ND+++Y+ +KVS ++G++N
Sbjct: 371 NALRNRIEEVIKGQAEVYRCSAEVEFFGEEHPTIPPTTNDERIYQLARKVSSMIVGEENI 550
Query: 181 RVVPPMMGAEDYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHAT 240
++ P + G+ED++FY + +P +FF++GI+NE GS ++ HSP F IDED LPIGAA+HA
Sbjct: 551 KLSPIVTGSEDFAFYLEKVPGSFFFLGIKNEKSGSIYSAHSPQFFIDEDVLPIGAAIHAA 730
Query: 241 IAERY 245
A Y
Sbjct: 731 FALSY 745
>AW980900 weakly similar to GP|18389266|gb IAA-amino acid hydrolase
{Arabidopsis thaliana}, partial (23%)
Length = 629
Score = 249 bits (637), Expect = 5e-67
Identities = 120/120 (100%), Positives = 120/120 (100%)
Frame = +3
Query: 131 IVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNFRVVPPMMGAE 190
IVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNFRVVPPMMGAE
Sbjct: 3 IVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNFRVVPPMMGAE 182
Query: 191 DYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHATIAERYLNEHG 250
DYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHATIAERYLNEHG
Sbjct: 183 DYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHATIAERYLNEHG 362
>TC77457 similar to PIR|F96556|F96556 IAA-Ala hydrolase (IAR3) [imported] -
Arabidopsis thaliana, partial (76%)
Length = 1420
Score = 247 bits (630), Expect = 3e-66
Identities = 123/246 (50%), Positives = 169/246 (68%)
Frame = +3
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
++ GALE+V AIF +HV P G + SR GP+ AGCGFF AVISG A P ++ D
Sbjct: 612 ILDTGALENVSAIFGLHVLSNLPLGEVASRSGPMAAGCGFFEAVISGMGGHGAIPHHAID 791
Query: 61 PVLAASAAVISIQGIVSRESNPLDSQVVSVTSFNGGNSHDMIPDSVVIGGTFRAFSNTSF 120
P+LAAS V+S+Q IVSRE +P+DSQVV+V F GG + ++IPDSV IGGTFRAFS SF
Sbjct: 792 PILAASNVVVSLQQIVSREVDPVDSQVVTVGKFQGGGAFNVIPDSVTIGGTFRAFSRESF 971
Query: 121 YQLLERIEQVIVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNF 180
L RIEQVI QA+V+ C A V+F E+E PPTVN+ ++++ + V+ LLG
Sbjct: 972 THLRHRIEQVITGQAAVHRCNATVNFLEEETPFIPPTVNNGGLHDYFESVAGRLLGVDKI 1151
Query: 181 RVVPPMMGAEDYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHAT 240
+ P +G+ED++FY + IP F +G+ + ++ +GHSP+F ++EDALP GAA+HA+
Sbjct: 1152KDQQPTVGSEDFAFYQEAIPGYIFLLGMEDVSIERLPSGHSPYFKVNEDALPYGAALHAS 1331
Query: 241 IAERYL 246
+A RYL
Sbjct: 1332LASRYL 1349
>TC87843 weakly similar to GP|3420801|gb|AAC31939.1| IAA-amino acid
hydrolase homolog ILL3 {Arabidopsis thaliana}, partial
(65%)
Length = 1339
Score = 231 bits (590), Expect = 2e-61
Identities = 119/249 (47%), Positives = 160/249 (63%)
Frame = +1
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
MI+DG L+DVEAIFAVH+ TG I S PGP A F A I G AA P + D
Sbjct: 484 MIKDGVLQDVEAIFAVHIDATTSTGAIASIPGPFTAAGCIFEAKIEGVGGHAAFPHQTVD 663
Query: 61 PVLAASAAVISIQGIVSRESNPLDSQVVSVTSFNGGNSHDMIPDSVVIGGTFRAFSNTSF 120
P+LA S A++++Q +VSRE +PL SQV+SVT GG++ ++IP V GGT R+ +
Sbjct: 664 PLLATSLAILALQQLVSREIDPLHSQVLSVTYIKGGDALNVIPSYVKFGGTLRSQTTEGM 843
Query: 121 YQLLERIEQVIVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNF 180
Y +R++++I QASV+ C A VDF E+ +T YP VND ++ HV++V +LG N
Sbjct: 844 YHFRQRLKEIIEGQASVHRCNAYVDFKEEAFTPYPAVVNDKDLHLHVERVGRLMLGPDNV 1023
Query: 181 RVVPPMMGAEDYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHAT 240
M ED++FY +VIP F IGIRN+ +GS H+ HSP F +DE+AL IGAA+H
Sbjct: 1024HEAKKAMVGEDFAFYQEVIPGVLFSIGIRNKKVGSIHSPHSPFFFLDEEALSIGAALHTA 1203
Query: 241 IAERYLNEH 249
+AE YLNEH
Sbjct: 1204VAELYLNEH 1230
>TC80526 weakly similar to GP|18844936|dbj|BAB85405. putative IAA-Ala
hydrolase {Oryza sativa (japonica cultivar-group)},
partial (61%)
Length = 1510
Score = 125 bits (315), Expect(3) = 6e-61
Identities = 55/119 (46%), Positives = 85/119 (71%)
Frame = +1
Query: 127 IEQVIVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLGQKNFRVVPPM 186
IE+VI QA V+ C AEV+FF KE+ PPT ND+++++ ++ S ++G++N ++ P
Sbjct: 976 IEEVIKGQAKVHRCTAEVEFFGKEHPTIPPTTNDERIHQLGRQASSMIVGEENIKLAPTY 1155
Query: 187 MGAEDYSFYSQVIPSAFFYIGIRNETLGSTHTGHSPHFTIDEDALPIGAAVHATIAERY 245
+ED++FY + +P +FF +GI+NE +GS ++ HSPH+ IDED LPIGAA+HA A Y
Sbjct: 1156 TASEDFAFYLEKVPGSFFLLGIQNEKVGSIYSAHSPHYFIDEDVLPIGAAIHAAFALSY 1332
Score = 84.0 bits (206), Expect(3) = 6e-61
Identities = 42/72 (58%), Positives = 54/72 (74%)
Frame = +3
Query: 55 PRNSADPVLAASAAVISIQGIVSRESNPLDSQVVSVTSFNGGNSHDMIPDSVVIGGTFRA 114
P++ DPVLAAS +VIS+Q IVSRE +PLDSQVVSV + ++H++IPDSV GGT+RA
Sbjct: 759 PQHCLDPVLAASMSVISLQNIVSREVDPLDSQVVSVAMVHSESAHELIPDSVTFGGTYRA 938
Query: 115 FSNTSFYQLLER 126
S SF L +R
Sbjct: 939 ISKKSFNALRQR 974
Score = 62.8 bits (151), Expect(3) = 6e-61
Identities = 27/47 (57%), Positives = 36/47 (76%)
Frame = +2
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISG 47
MIQ+ LEDVEAIF +H++ ++P G++ SRPG LAGCG F+A I G
Sbjct: 602 MIQENVLEDVEAIFGLHLATQYPLGVVASRPGDFLAGCGSFKAKIKG 742
>TC89442 similar to GP|17978838|gb|AAL47552.1 IAA-amino acid conjugate
hydrolase-like protein {Arabidopsis thaliana}, partial
(39%)
Length = 973
Score = 147 bits (372), Expect = 3e-36
Identities = 73/75 (97%), Positives = 75/75 (99%)
Frame = +2
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD
Sbjct: 749 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 928
Query: 61 PVLAASAAVISIQGI 75
PVLAASAAVI++QGI
Sbjct: 929 PVLAASAAVINLQGI 973
>BE123972 similar to PIR|F96556|F96 IAA-Ala hydrolase (IAR3) [imported] -
Arabidopsis thaliana, partial (46%)
Length = 615
Score = 95.5 bits (236), Expect(2) = 3e-30
Identities = 47/86 (54%), Positives = 60/86 (69%)
Frame = +1
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
++ GALE+V AIF +HV P G + SR GP+ AGCGFF AVISG A P ++ D
Sbjct: 229 ILDTGALENVSAIFGLHVLSNLPLGEVASRSGPMAAGCGFFEAVISGMGGHGAIPHHAID 408
Query: 61 PVLAASAAVISIQGIVSRESNPLDSQ 86
P+LAAS V+S+Q IVSRE +P+DSQ
Sbjct: 409 PILAASNVVVSLQQIVSREVDPVDSQ 486
Score = 53.5 bits (127), Expect(2) = 3e-30
Identities = 26/39 (66%), Positives = 29/39 (73%)
Frame = +3
Query: 93 FNGGNSHDMIPDSVVIGGTFRAFSNTSFYQLLERIEQVI 131
F GG + ++IPDSV IGGTFRAFS SF L RIEQVI
Sbjct: 492 FQGGGAFNVIPDSVTIGGTFRAFSRESFTHLRHRIEQVI 608
>BE239385 similar to GP|18844936|dbj putative IAA-Ala hydrolase {Oryza sativa
(japonica cultivar-group)}, partial (20%)
Length = 592
Score = 120 bits (301), Expect = 5e-28
Identities = 62/133 (46%), Positives = 89/133 (66%), Gaps = 2/133 (1%)
Frame = +2
Query: 119 SFYQLLERIEQVIVQQASVYSCFAEVDFFEKEYTIYPPTVNDDQMYEHVKKVSIDLLG-Q 177
SF QL +RIE+VI+ QA+V+ C A VDF YPPTVN+ ++EH V++++LG
Sbjct: 2 SFNQLRQRIEEVIIGQAAVHRCNATVDFLHGVKPFYPPTVNNADLHEHFVNVAVNMLGID 181
Query: 178 KNFRVVPPMMGAEDYSFYSQVIPSAFFYIGIRNETLGSTHT-GHSPHFTIDEDALPIGAA 236
K V+ P MG+ED+SFY +VIP FF +G++N + + HSP+ I+ED LP GAA
Sbjct: 182 KVDSVMTPYMGSEDFSFYQEVIPGYFFMLGVKNASHKRFESLLHSPYLEINEDGLPYGAA 361
Query: 237 VHATIAERYLNEH 249
+HA++A YL +H
Sbjct: 362 LHASLAASYLLKH 400
>TC79815 similar to GP|2181184|emb|CAA73905.1 JR3 protein {Arabidopsis
thaliana}, partial (46%)
Length = 850
Score = 71.6 bits (174), Expect = 3e-13
Identities = 35/65 (53%), Positives = 45/65 (68%)
Frame = +2
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSAD 60
++ GALE+V AIF +H+ + P G + SR GP+LAG GFF A ISGK AA P+ S D
Sbjct: 656 ILDAGALENVTAIFGLHIVPDLPIGEVSSRSGPILAGSGFFEAKISGKGGHAAIPQQSID 835
Query: 61 PVLAA 65
P+LAA
Sbjct: 836 PILAA 850
>BG587186 similar to GP|18844936|dbj putative IAA-Ala hydrolase {Oryza sativa
(japonica cultivar-group)}, partial (11%)
Length = 598
Score = 58.2 bits (139), Expect = 3e-09
Identities = 27/52 (51%), Positives = 37/52 (70%)
Frame = +2
Query: 86 QVVSVTSFNGGNSHDMIPDSVVIGGTFRAFSNTSFYQLLERIEQVIVQQASV 137
QVV++ GG++ ++IPD V IGGTFRAFS SF QL +RIE+V + S+
Sbjct: 38 QVVTIAKIQGGSAFNVIPDHVTIGGTFRAFSKESFNQLRQRIEEVSIGNKSI 193
>BF645682 similar to GP|7270509|emb| NAM / CUC2-like protein {Arabidopsis
thaliana}, partial (17%)
Length = 668
Score = 45.1 bits (105), Expect = 3e-05
Identities = 24/41 (58%), Positives = 27/41 (65%), Gaps = 1/41 (2%)
Frame = +1
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPL-LAGCGF 40
MI++G L+DVEAIFAVH TG I S PGP AGC F
Sbjct: 262 MIKEGVLQDVEAIFAVHTDATASTGNIASIPGPFTAAGCIF 384
>AW736524 similar to GP|2181184|emb JR3 protein {Arabidopsis thaliana},
partial (22%)
Length = 637
Score = 42.0 bits (97), Expect = 2e-04
Identities = 18/39 (46%), Positives = 27/39 (69%)
Frame = +1
Query: 1 MIQDGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCG 39
++ GALE+V AIF +H+ + P G + S+ GP+LAG G
Sbjct: 520 ILDAGALENVTAIFGLHIVPDLPIGEVSSKSGPILAGSG 636
>TC91251 similar to PIR|T00553|T00553 disease resistance response protein
homolog F12L6.9 - Arabidopsis thaliana, partial (41%)
Length = 971
Score = 30.8 bits (68), Expect = 0.51
Identities = 20/52 (38%), Positives = 26/52 (49%), Gaps = 1/52 (1%)
Frame = +2
Query: 66 SAAVISIQGIVSRESNPLDSQVVSVTSF-NGGNSHDMIPDSVVIGGTFRAFS 116
S V QG + S SQ V +T F +GGN HD + DS+ + G R S
Sbjct: 356 SGVVGRAQGFYTASSLDGTSQSVVMTVFLHGGNDHDGVDDSISLFGVHRTAS 511
>TC77091 weakly similar to GP|11602751|emb|CAC18558. ENOD18 protein {Vicia
faba}, partial (67%)
Length = 896
Score = 28.9 bits (63), Expect = 1.9
Identities = 35/135 (25%), Positives = 53/135 (38%)
Frame = +3
Query: 4 DGALEDVEAIFAVHVSHEHPTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRNSADPVL 63
D L + ++ VHV+H HPT L A G +S R + DP
Sbjct: 180 DNLLRTGDTLYIVHVNHSHPT----ESRNLLWATTGSPLIPLSEFREKNVVHQYEVDP-- 341
Query: 64 AASAAVISIQGIVSRESNPLDSQVVSVTSFNGGNSHDMIPDSVVIGGTFRAFSNTSFYQL 123
A V+ I SR+ QV V G++ + I DSV G + + +
Sbjct: 342 --DAEVLDILDTASRQ-----KQVTVVGKVYWGDAREKIVDSV---GDLKLDALVMGSRG 491
Query: 124 LERIEQVIVQQASVY 138
L I++V++ S Y
Sbjct: 492 LGAIQRVLLGSVSTY 536
>TC77570 similar to PIR|H96751|H96751 probable casein kinase I F28P22.10
[imported] - Arabidopsis thaliana, partial (72%)
Length = 1989
Score = 27.7 bits (60), Expect = 4.3
Identities = 21/69 (30%), Positives = 32/69 (45%), Gaps = 4/69 (5%)
Frame = +3
Query: 23 PTGMIGSRPGPLLAGCGFFRAVISGKRASAANPRN----SADPVLAASAAVISIQGIVSR 78
P+ IG GP +G A G+ + RN S+DP ++ I+ GI+SR
Sbjct: 1026 PSRGIGLAAGPS-SGLPLASANADGQSGGGKDGRNIGWSSSDPTRRRTSGPIANDGILSR 1202
Query: 79 ESNPLDSQV 87
E PL + +
Sbjct: 1203 EKAPLTNDL 1229
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,811,397
Number of Sequences: 36976
Number of extensions: 83886
Number of successful extensions: 378
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 374
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 375
length of query: 250
length of database: 9,014,727
effective HSP length: 94
effective length of query: 156
effective length of database: 5,538,983
effective search space: 864081348
effective search space used: 864081348
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC140916.4