Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140916.1 - phase: 0 
         (108 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC81567 similar to PIR|T47764|T47764 responce reactor 4 - Arabid...   178  2e-46
BI270332 similar to PIR|T06673|T066 response reactor 2 [imported...   110  4e-40
TC78129 similar to PIR|T47764|T47764 responce reactor 4 - Arabid...    65  4e-12
TC81494 similar to GP|13173408|gb|AAK14395.1 response regulator ...    64  9e-12
BE249457 similar to PIR|T50854|T508 response regulator 7 [import...    59  3e-10
BE248753 homologue to GP|11870065|gb| response regulator 15 {Ara...    51  8e-08
TC78934 similar to GP|14189890|dbj|BAB55874. response regulator ...    32  0.038
TC80227 similar to GP|13537198|dbj|BAB40775. histidine kinase {A...    31  0.084
TC77644 similar to GP|16751567|gb|AAL27697.1 5-enolpyruvylshikim...    30  0.14
CA990978 weakly similar to GP|12002863|gb|A self-pruning interac...    29  0.32
TC81488 GP|17985051|gb|AAL54093.1 EXOPOLYSACCHARIDE PRODUCTION P...    28  0.42
TC79846 similar to GP|8843728|dbj|BAA97276.1 homeodomain transcr...    28  0.55
TC85641 weakly similar to GP|8777295|dbj|BAA96885.1 cytochrome P...    28  0.71
AL367145 weakly similar to GP|8096269|dbj| KED {Nicotiana tabacu...    27  0.93
TC79389 similar to GP|14189890|dbj|BAB55874. response regulator ...    27  0.93
TC80575 weakly similar to GP|12056928|gb|AAG48132.1 putative res...    27  1.6
TC80307 similar to GP|20160990|dbj|BAB89924. putative protein ki...    26  2.1
TC93645 similar to GP|19699053|gb|AAL90894.1 AT5g23390/T32G24_2 ...    26  2.1
TC78112 similar to PIR|C96673|C96673 gamma-tocopherol methyltran...    26  2.1
TC80605 similar to PIR|S56716|S56716 protein kinase SPK-3 (EC 2....    25  3.5

>TC81567 similar to PIR|T47764|T47764 responce reactor 4 - Arabidopsis
           thaliana, partial (27%)
          Length = 470

 Score =  178 bits (452), Expect = 2e-46
 Identities = 91/91 (100%), Positives = 91/91 (100%)
 Frame = +3

Query: 1   MELGLDSRKLQLQPQNLKQEHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKY 60
           MELGLDSRKLQLQPQNLKQEHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKY
Sbjct: 36  MELGLDSRKLQLQPQNLKQEHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKY 215

Query: 61  LGLNIDEISSTKSSILESSSPSLPQPLQLQE 91
           LGLNIDEISSTKSSILESSSPSLPQPLQLQE
Sbjct: 216 LGLNIDEISSTKSSILESSSPSLPQPLQLQE 308


>BI270332 similar to PIR|T06673|T066 response reactor 2 [imported] -
           Arabidopsis thaliana, partial (47%)
          Length = 556

 Score =  110 bits (275), Expect(2) = 4e-40
 Identities = 56/56 (100%), Positives = 56/56 (100%)
 Frame = +2

Query: 36  LLERLLRDSSCKVTCVDSGDKALKYLGLNIDEISSTKSSILESSSPSLPQPLQLQE 91
           LLERLLRDSSCKVTCVDSGDKALKYLGLNIDEISSTKSSILESSSPSLPQPLQLQE
Sbjct: 101 LLERLLRDSSCKVTCVDSGDKALKYLGLNIDEISSTKSSILESSSPSLPQPLQLQE 268



 Score = 68.9 bits (167), Expect(2) = 4e-40
 Identities = 33/33 (100%), Positives = 33/33 (100%)
 Frame = +1

Query: 3  LGLDSRKLQLQPQNLKQEHFHVLAVDDSVIDRM 35
          LGLDSRKLQLQPQNLKQEHFHVLAVDDSVIDRM
Sbjct: 1  LGLDSRKLQLQPQNLKQEHFHVLAVDDSVIDRM 99


>TC78129 similar to PIR|T47764|T47764 responce reactor 4 - Arabidopsis
           thaliana, partial (62%)
          Length = 1307

 Score = 65.1 bits (157), Expect = 4e-12
 Identities = 34/62 (54%), Positives = 45/62 (71%), Gaps = 1/62 (1%)
 Frame = +1

Query: 19  QEHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGL-NIDEISSTKSSILE 77
           +  FHVLAVDDS+IDR L+ERLL+ SS +VT VDSG KAL++LGL   DE +    S+  
Sbjct: 394 ESQFHVLAVDDSIIDRKLIERLLKTSSYQVTTVDSGSKALEFLGLCENDETNPNTPSVFP 573

Query: 78  SS 79
           ++
Sbjct: 574 NN 579


>TC81494 similar to GP|13173408|gb|AAK14395.1 response regulator protein
           {Dianthus caryophyllus}, partial (53%)
          Length = 793

 Score = 63.9 bits (154), Expect = 9e-12
 Identities = 31/54 (57%), Positives = 45/54 (82%)
 Frame = +1

Query: 20  EHFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNIDEISSTKS 73
           +  HVLAVDDS++DR ++ERLL+ S+CKVT VDSG +AL++LGL +DE  +++S
Sbjct: 82  QEVHVLAVDDSLVDRKVIERLLKISACKVTAVDSGIRALQFLGL-VDEKETSES 240


>BE249457 similar to PIR|T50854|T508 response regulator 7 [imported] -
           Arabidopsis thaliana, partial (21%)
          Length = 246

 Score = 58.9 bits (141), Expect = 3e-10
 Identities = 27/42 (64%), Positives = 37/42 (87%)
 Frame = +2

Query: 23  HVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLN 64
           HVLAVDDS++DR ++ERLL+ SSCKVT V+S  +AL++LGL+
Sbjct: 116 HVLAVDDSLVDRKVIERLLKISSCKVTVVESARRALQFLGLD 241


>BE248753 homologue to GP|11870065|gb| response regulator 15 {Arabidopsis
           thaliana}, partial (22%)
          Length = 257

 Score = 50.8 bits (120), Expect = 8e-08
 Identities = 23/35 (65%), Positives = 30/35 (85%)
 Frame = +2

Query: 23  HVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKA 57
           HVLAVDDS +DR ++ERLL+ +SCKVT V+SG +A
Sbjct: 122 HVLAVDDSFVDRKVIERLLKFTSCKVTVVESGTRA 226


>TC78934 similar to GP|14189890|dbj|BAB55874. response regulator 9 {Zea
           mays}, partial (25%)
          Length = 1014

 Score = 32.0 bits (71), Expect = 0.038
 Identities = 20/43 (46%), Positives = 21/43 (48%)
 Frame = +2

Query: 24  VLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNID 66
           VLAVDD     +LLE LLR     VT       AL  L  NID
Sbjct: 68  VLAVDDDPTSLLLLETLLRSCQYHVTTTSEAITALTMLQENID 196


>TC80227 similar to GP|13537198|dbj|BAB40775. histidine kinase {Arabidopsis
           thaliana}, partial (22%)
          Length = 1693

 Score = 30.8 bits (68), Expect = 0.084
 Identities = 19/60 (31%), Positives = 27/60 (44%), Gaps = 4/60 (6%)
 Frame = +3

Query: 6   DSRKLQLQPQNLKQEHF----HVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYL 61
           + R L    QNL   H      +L VDD+ ++R +    L+     V CV SG  A+  L
Sbjct: 702 NKRTLNRDLQNLSLSHLLVGRKILIVDDNGVNRAVAAGALKKYGADVVCVSSGKDAISSL 881


>TC77644 similar to GP|16751567|gb|AAL27697.1
           5-enolpyruvylshikimate-3-phosphate synthase {Dicliptera
           chinensis}, partial (41%)
          Length = 1342

 Score = 30.0 bits (66), Expect = 0.14
 Identities = 20/62 (32%), Positives = 35/62 (56%), Gaps = 6/62 (9%)
 Frame = +1

Query: 30  SVIDRMLLERLLRDSSCKV-TCVDSGD-----KALKYLGLNIDEISSTKSSILESSSPSL 83
           S+ +R+LL   L + +  V   +DS D     +ALK LGL +++  +TK +++E S    
Sbjct: 535 SLSNRILLLAALSEGTTVVENLLDSEDIHYMLEALKTLGLRVEDDKTTKQAVVEGSGGLF 714

Query: 84  PQ 85
           P+
Sbjct: 715 PE 720


>CA990978 weakly similar to GP|12002863|gb|A self-pruning interacting protein
           1 {Lycopersicon esculentum}, partial (24%)
          Length = 657

 Score = 28.9 bits (63), Expect = 0.32
 Identities = 15/46 (32%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
 Frame = +2

Query: 49  TCVDSGDKALKYLGLNIDEI-SSTKSSILESSSPSLPQPLQLQEVI 93
           TC+  G K  K+L +  + I    K    E    ++P+P+Q++EVI
Sbjct: 119 TCLQQGQKRHKFLVICEESIIKQEKREQEEEEEANIPKPIQVEEVI 256


>TC81488 GP|17985051|gb|AAL54093.1 EXOPOLYSACCHARIDE PRODUCTION PROTEIN EXOF
           PRECURSOR {Brucella melitensis}, partial (3%)
          Length = 921

 Score = 28.5 bits (62), Expect = 0.42
 Identities = 12/31 (38%), Positives = 22/31 (70%)
 Frame = +3

Query: 30  SVIDRMLLERLLRDSSCKVTCVDSGDKALKY 60
           S I  +++  +LRDSSC ++C+ + D A++Y
Sbjct: 513 SRISSLVIAPILRDSSCFISCI-AADNAVRY 602


>TC79846 similar to GP|8843728|dbj|BAA97276.1 homeodomain transcription
           factor-like {Arabidopsis thaliana}, partial (42%)
          Length = 679

 Score = 28.1 bits (61), Expect = 0.55
 Identities = 23/88 (26%), Positives = 45/88 (51%), Gaps = 14/88 (15%)
 Frame = +2

Query: 1   MELGLDSRKLQLQPQN---------LKQEHFHVLAVDDS-VIDRMLLERLLRDSSCKV-- 48
           MELGLD R++ +  QN         L++E+F +    +S ++++ LLE  LR+   +   
Sbjct: 356 MELGLDPRQVAVWFQNRRARWKNKKLEEEYFSLKKNHESTILEKCLLETKLREQHSEALK 535

Query: 49  --TCVDSGDKALKYLGLNIDEISSTKSS 74
               +   +K ++ L    + ++S+ SS
Sbjct: 536 LREQLSEAEKEIQRLREPFERVTSSSSS 619


>TC85641 weakly similar to GP|8777295|dbj|BAA96885.1 cytochrome P450-like
            {Arabidopsis thaliana}, partial (71%)
          Length = 1941

 Score = 27.7 bits (60), Expect = 0.71
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -1

Query: 21   HFHVLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNI 65
            +FH+L +D  +  R L E   ++S+C  + V S D+  K L  NI
Sbjct: 1077 NFHLLLIDFIINVR*LAEIFDKESACDASSVMSSDQKAKNLISNI 943


>AL367145 weakly similar to GP|8096269|dbj| KED {Nicotiana tabacum}, partial
           (8%)
          Length = 284

 Score = 27.3 bits (59), Expect = 0.93
 Identities = 15/37 (40%), Positives = 20/37 (53%)
 Frame = +2

Query: 69  SSTKSSILESSSPSLPQPLQLQEVIILIFFFLILKSF 105
           SS   ++L S   S P  L L    +L+FFFL+L  F
Sbjct: 158 SSFCFNLLTSLDLSFPS*LSLLSTFVLVFFFLLLFHF 268


>TC79389 similar to GP|14189890|dbj|BAB55874. response regulator 9 {Zea
           mays}, partial (33%)
          Length = 1532

 Score = 27.3 bits (59), Expect = 0.93
 Identities = 19/52 (36%), Positives = 23/52 (43%)
 Frame = +1

Query: 24  VLAVDDSVIDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNIDEISSTKSSI 75
           VLAVDD     ++LE LLR     VT       AL  L  N D+     S +
Sbjct: 211 VLAVDDDPTCLLVLETLLRRCQYHVTTTSQAITALTMLRENKDKFDLVISDV 366


>TC80575 weakly similar to GP|12056928|gb|AAG48132.1 putative resistance
           protein {Glycine max}, partial (5%)
          Length = 758

 Score = 26.6 bits (57), Expect = 1.6
 Identities = 14/46 (30%), Positives = 24/46 (51%)
 Frame = -3

Query: 32  IDRMLLERLLRDSSCKVTCVDSGDKALKYLGLNIDEISSTKSSILE 77
           + + ++   L++ SCK      G K L  L LN+ +ISS    ++E
Sbjct: 135 LTQSVISNFLKEGSCK------GGKCLSSLQLNVSKISSLFRRVME 16


>TC80307 similar to GP|20160990|dbj|BAB89924. putative protein kinase {Oryza
            sativa (japonica cultivar-group)}, partial (69%)
          Length = 1929

 Score = 26.2 bits (56), Expect = 2.1
 Identities = 11/32 (34%), Positives = 21/32 (65%)
 Frame = -3

Query: 64   NIDEISSTKSSILESSSPSLPQPLQLQEVIIL 95
            N+D + + + +++ +S+ SLP PL L E + L
Sbjct: 1600 NLDSLQTARLALIGNSTTSLPCPLGLVEALEL 1505


>TC93645 similar to GP|19699053|gb|AAL90894.1 AT5g23390/T32G24_2
           {Arabidopsis thaliana}, partial (26%)
          Length = 994

 Score = 26.2 bits (56), Expect = 2.1
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = -2

Query: 86  PLQLQEVIILIFFFLILKSFQL 107
           PL LQE +++ FFFL L+ + +
Sbjct: 579 PLLLQETLLIAFFFLHLQKYHV 514


>TC78112 similar to PIR|C96673|C96673 gamma-tocopherol methyltransferase
           [imported] - Arabidopsis thaliana, partial (64%)
          Length = 1100

 Score = 26.2 bits (56), Expect = 2.1
 Identities = 15/42 (35%), Positives = 21/42 (49%)
 Frame = -2

Query: 58  LKYLGLNIDEISSTKSSILESSSPSLPQPLQLQEVIILIFFF 99
           L  L + ID ++   SS+L    PSL  P  L   + L FF+
Sbjct: 238 LHSLAILIDPLTEVSSSLLLPLPPSLLSPPPLIHAMNLFFFY 113


>TC80605 similar to PIR|S56716|S56716 protein kinase SPK-3 (EC 2.7.1.-) -
           soybean, partial (25%)
          Length = 533

 Score = 25.4 bits (54), Expect = 3.5
 Identities = 12/19 (63%), Positives = 15/19 (78%)
 Frame = -2

Query: 69  SSTKSSILESSSPSLPQPL 87
           SS+KSS   +SSPS P+PL
Sbjct: 202 SSSKSSSPSTSSPSQPKPL 146


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.322    0.139    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,265,055
Number of Sequences: 36976
Number of extensions: 41047
Number of successful extensions: 343
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 343
length of query: 108
length of database: 9,014,727
effective HSP length: 84
effective length of query: 24
effective length of database: 5,908,743
effective search space: 141809832
effective search space used: 141809832
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)


Medicago: description of AC140916.1