BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140913.11 - phase: 0
(803 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW736388 similar to GP|10178015|db Na+/H+ antiporter-like protei... 337 8e-93
TC82389 similar to PIR|T45912|T45912 hypothetical protein F5K20.... 270 2e-72
CA921498 similar to PIR|T45912|T45 hypothetical protein F5K20.20... 231 8e-61
TC83096 weakly similar to PIR|T45912|T45912 hypothetical protein... 196 2e-50
AL376050 similar to GP|10178015|dbj Na+/H+ antiporter-like prote... 158 9e-39
TC83471 similar to PIR|T45912|T45912 hypothetical protein F5K20.... 132 4e-31
AW774205 similar to PIR|T45912|T459 hypothetical protein F5K20.2... 109 5e-24
TC87017 similar to PIR|A96564|A96564 unknown protein 23094-2177... 32 0.74
>AW736388 similar to GP|10178015|db Na+/H+ antiporter-like protein
{Arabidopsis thaliana}, partial (21%)
Length = 558
Score = 337 bits (865), Expect = 8e-93
Identities = 173/184 (94%), Positives = 179/184 (97%)
Frame = +3
Query: 125 GRVLAIAIAGISLPFALGIGSSFVLQRTIAKGVNTSAFLVYMGVALSITAFPVLARILAE 184
GRVLAI++ GISLPFALGIGSSFVL+ TIAK VNTSAFLVYMGVALSITAFPVLARILAE
Sbjct: 6 GRVLAISMVGISLPFALGIGSSFVLKETIAKDVNTSAFLVYMGVALSITAFPVLARILAE 185
Query: 185 LKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSPFVSLWVFLSGCGFVVCSILI 244
LKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSP VSLWVFL+GCGFVVCSILI
Sbjct: 186 LKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSPLVSLWVFLAGCGFVVCSILI 365
Query: 245 VLPIFKWMAQQCHEGEPVDELYICATLAAVLAAGFVTDAIGIHAMFGAFVFGILVPKDGA 304
VLPIFKWMAQQCHEGEPVDELYICATLA+VLAAGFVTDAIGIHAMFGAFVFGILVPKDG
Sbjct: 366 VLPIFKWMAQQCHEGEPVDELYICATLASVLAAGFVTDAIGIHAMFGAFVFGILVPKDGP 545
Query: 305 FAGA 308
F+GA
Sbjct: 546 FSGA 557
>TC82389 similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (23%)
Length = 907
Score = 270 bits (689), Expect = 2e-72
Identities = 140/269 (52%), Positives = 198/269 (73%), Gaps = 9/269 (3%)
Frame = +3
Query: 16 QPASNGVFQGDHPLDYALPLAILQICLVLVVTRGLAYLLNPLRQPRVIAEIVGGILLGPS 75
+ +S+G +QGD+P+++ALPL I+Q LV+ V+R LA+ L PLRQPRV+AEI+GGILLGPS
Sbjct: 105 ETSSDGAWQGDNPMNHALPLLIIQTILVMFVSRTLAFFLKPLRQPRVVAEIIGGILLGPS 284
Query: 76 ALGRNKGYLHAVFPPKSLPVLDTLANLGLIFFLFLAGIELDPKSLRKTGGRVLAIAIAGI 135
+GRNK ++H VFP S P+L+++A++GL+F+LFL G+ELD +++ ++G R IA+AGI
Sbjct: 285 GIGRNKTFMHTVFPSWSTPILESVASIGLLFYLFLVGLELDLRTINRSGKRAFNIAVAGI 464
Query: 136 SLPFALGIGSSFVLQRTI-----AKGVNTSAFLVYMGVALSITAFPVLARILAELKLLTT 190
SLPF IG +F+LQ+ I V+ +++GV+LSITAFPVLARILAELKLLTT
Sbjct: 465 SLPFLFAIGVTFLLQKVIHFNSETHKVSYFQLFIFLGVSLSITAFPVLARILAELKLLTT 644
Query: 191 SVGRMAMSAAAVNDVAAWILLALAVALSGNSQ---SPFVSLWVFLSGCGFVVCSILIVLP 247
VG AM+AAA NDVAAW+LLALA+AL+G + S+ V LSG F V + ++ P
Sbjct: 645 QVGETAMAAAAFNDVAAWVLLALAIALAGGGEHRNGVLTSILVLLSGVAFGV--LFVIRP 818
Query: 248 IFKWMAQQC-HEGEPVDELYICATLAAVL 275
+ + ++ +C E E +DE++IC TLA V+
Sbjct: 819 LMERISLRCSRETEVLDEMFICLTLAGVM 905
>CA921498 similar to PIR|T45912|T45 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (23%)
Length = 658
Score = 231 bits (589), Expect = 8e-61
Identities = 118/191 (61%), Positives = 151/191 (78%), Gaps = 2/191 (1%)
Frame = +2
Query: 15 MQPASNGVFQGDHPLDYALPLAILQICLVLVVTRGLAYLLNPLRQPRVIAEIVGGILLGP 74
++ +S+G++QGD+PLDYA PL I+Q LVLVV+R LA+ PLRQP+VIAEI+GGILLGP
Sbjct: 86 IKTSSDGIWQGDNPLDYAFPLLIIQTVLVLVVSRSLAFGFKPLRQPKVIAEIIGGILLGP 265
Query: 75 SALGRNKGYLHAVFPPKSLPVLDTLANLGLIFFLFLAGIELDPKSLRKTGGRVLAIAIAG 134
SALGRN YLH +FP S+P L+++A++GL+FFLFL G+ELD S+R++G R +IA G
Sbjct: 266 SALGRNTSYLHRLFPEWSMPTLESVASIGLLFFLFLVGLELDLNSIRRSGKRAFSIAACG 445
Query: 135 ISLPFALGIGSSFVLQRTI--AKGVNTSAFLVYMGVALSITAFPVLARILAELKLLTTSV 192
I+LPF GIG + VL++T+ A F+V+MGVALSITAFPVLARILAELKLLTT V
Sbjct: 446 ITLPFVCGIGVAIVLRKTVDGADKAGFGQFIVFMGVALSITAFPVLARILAELKLLTTRV 625
Query: 193 GRMAMSAAAVN 203
G AM+AAA N
Sbjct: 626 GETAMAAAAFN 658
>TC83096 weakly similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (21%)
Length = 1227
Score = 196 bits (499), Expect = 2e-50
Identities = 133/369 (36%), Positives = 201/369 (54%), Gaps = 14/369 (3%)
Frame = +2
Query: 426 VLAAYK--RKERKSNYKYRTIERKNADGQLRILACFHGSRNIPSLINLIEASRGIKKHDA 483
V+A YK R+ R N T + +LRILAC HG+ NIPSLIN IE+ R K
Sbjct: 2 VVAIYKPSRQRRSGNPPPLT----DTQEKLRILACIHGTGNIPSLINFIESVRATNKSSK 169
Query: 484 LCVYAMHLKEFCERSSSILMAQKVRQNGLPFWDKGRHGDSLHVIVAFEAYQKLSQVFVRP 543
+ +Y M L E + SSSILM + R++G PF ++ + G AF A ++ QV V
Sbjct: 170 IKLYVMQLTELTDSSSSILMVRSSRKSGFPFINRFQKGTMQE---AFRACGQVGQVTVHH 340
Query: 544 MIAISSMANIHEDICTTADRKRAAVIILPFHKQQRVDG--SLDIIRNDFRLVNKRVLEHA 601
+ +ISS++ IHEDIC A+ K A+IILPFHK+ R + +++ I +R VN+RVL+ A
Sbjct: 341 LTSISSLSTIHEDICHIAEEKGVAMIILPFHKRWRGEDEETIEDIGQRWREVNQRVLQSA 520
Query: 602 PCSVGIFVDRGLGGSCH---VSASNVSYCIAVLFFGGGDDREALAYGARMAEHPGIQLVV 658
PCSV + V+RG+G +++ + ++F GG DDR+ L G+RMAEHP I+L V
Sbjct: 521 PCSVAVLVNRGVGRRYEQRVETSATPGKKVCIIFVGGPDDRKVLELGSRMAEHPAIRLSV 700
Query: 659 IHFLV--EPNIAGKITKVDVGDSSSNNSISDSEDGEFLAEFKLKTANDDSIIYEERIVKD 716
+ F + E + + S+S+N++ + ++ + +A + KT ++ Y E +
Sbjct: 701 VRFNLHNEGTFRDQEHSYNTSTSASDNNMENEKELDEVALNEFKTKWLGAVEYIENDTVN 880
Query: 717 AEETVATIREINFCNLFLVGR-----RPAGELGFALERSECPELGPVGGLLASQDFRTTA 771
V I + L +VG+ G + + E ELGP+G LL S T+
Sbjct: 881 IANEVLAIGRVKEYELVIVGKGHQLLNSTGMIDIKDSQLEHAELGPIGDLLTSSAQGITS 1060
Query: 772 SVLVMQQYH 780
SVLV+Q H
Sbjct: 1061SVLVIQGQH 1087
>AL376050 similar to GP|10178015|dbj Na+/H+ antiporter-like protein
{Arabidopsis thaliana}, partial (3%)
Length = 521
Score = 158 bits (399), Expect = 9e-39
Identities = 80/102 (78%), Positives = 87/102 (84%), Gaps = 2/102 (1%)
Frame = +2
Query: 704 DDSIIYEERIVKDAEETVATIREINFCNLFLVGRRPAGELGFALERSECPELGPVGGLLA 763
DDS+IYEE+IVKDA ETVA+IR+ N CNLFLVG RP GEL ALER ECPELGPVGGLL
Sbjct: 2 DDSVIYEEKIVKDAAETVASIRKFNSCNLFLVGLRPTGELACALERRECPELGPVGGLLI 181
Query: 764 SQDFRTTASVLVMQQYHNGVPINFVPEMEE--HSRDGDTESS 803
SQD TTASVLVMQQYHNGVP+NFVPEMEE HS +GDT S+
Sbjct: 182 SQDCPTTASVLVMQQYHNGVPMNFVPEMEEHSHSHEGDT*SA 307
>TC83471 similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (18%)
Length = 983
Score = 132 bits (333), Expect = 4e-31
Identities = 97/273 (35%), Positives = 149/273 (54%), Gaps = 28/273 (10%)
Frame = +3
Query: 535 KLSQVFVRPMIAISSMANIHEDICTTADRKRAAVIILPFHKQQRVDGS----------LD 584
+L +V VR AISS++ +HEDIC A+ KR +IILPFHK R++ L+
Sbjct: 3 QLGRVIVRSTTAISSLSTMHEDICHAAEEKRVTMIILPFHKHWRMEVDDENDKEAHEVLE 182
Query: 585 IIRNDFRLVNKRVLEHAPCSVGIFVDR--GLGGSCHVSASNVSYCIAVLFFGGGDDREAL 642
+ +R VN+RVL++APCSV + VDR GLG S V+ I ++FFGG DDREAL
Sbjct: 183 NAGHGWRGVNQRVLKNAPCSVAVLVDRGYGLGLKNLGSDGRVAQRICIVFFGGPDDREAL 362
Query: 643 AYGARMAEHPGIQLVVIHFLVEPNIAGK--ITKVDVGDSSSNN-SISDSE---------D 690
G +M EHP + + V+ F+ + ++G + + G S+ N S S ++ D
Sbjct: 363 ELGKKMVEHPAVVVTVVRFVEQNELSGNNFVLRQSPGKSTEENYSFSIAKINRQKEQVLD 542
Query: 691 GEFLAEFKLKTANDDSIIYEERIVKDAEETVATIREINFCNLFLV--GRRPAGELGFALE 748
+ EF+ K +++ Y E+ + E V + E +L +V GR P+ + E
Sbjct: 543 ENAMEEFRSKCG--ETVKYIEKGSGNVVEEVIALGESADYDLIVVGKGRFPSTMVAELAE 716
Query: 749 R-SECPELGPVGGLL-ASQDFRTTASVLVMQQY 779
R +E ELGP+G +L +S + +SV V+QQ+
Sbjct: 717 REAEHAELGPIGDILTSSMGHKMASSVFVIQQH 815
>AW774205 similar to PIR|T45912|T459 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (9%)
Length = 322
Score = 109 bits (272), Expect = 5e-24
Identities = 52/85 (61%), Positives = 66/85 (77%)
Frame = +1
Query: 291 GAFVFGILVPKDGAFAGALVEKIEDLVSGLLLPLYFVSSGLKTDIATIQGLQSWGLLVFV 350
GAFVFG+ +PK G+FA L+E+IED V GLLLPLYF SSGLKTD+ I G ++ GLLV V
Sbjct: 1 GAFVFGLTIPKTGSFAERLIERIEDFVLGLLLPLYFASSGLKTDVTKISGGKARGLLVLV 180
Query: 351 TFTACFGKIVGTIVVSLICKVPFNE 375
TAC GKI+GT VV+++C++P E
Sbjct: 181 IATACAGKILGTFVVAMMCRMPVTE 255
Score = 33.9 bits (76), Expect = 0.25
Identities = 17/41 (41%), Positives = 24/41 (58%)
Frame = +2
Query: 357 GKIVGTIVVSLICKVPFNESLVLGFLMNSKGLVELIVLNIG 397
G+ G +++ P S+ LG LMN+KGL LI L+IG
Sbjct: 200 GRFWGLLLLL*CVGCPLQSSITLGVLMNTKGLCGLIALHIG 322
>TC87017 similar to PIR|A96564|A96564 unknown protein 23094-21772
[imported] - Arabidopsis thaliana, partial (30%)
Length = 1701
Score = 32.3 bits (72), Expect = 0.74
Identities = 32/146 (21%), Positives = 65/146 (43%)
Frame = -3
Query: 166 MGVALSITAFPVLARILAELKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSPF 225
+G +L++++ +L+ L+ L LL+ +++ + + V +L++ A++LS S
Sbjct: 751 VGSSLTLSSLSLLSLTLSSLSLLSL-FPFLSLFSVFFSSVGGSLLISPALSLSATS---- 587
Query: 226 VSLWVFLSGCGFVVCSILIVLPIFKWMAQQCHEGEPVDELYICATLAAVLAAGFVTDAIG 285
FLS ++ S+L L + + L++ AT +
Sbjct: 586 -----FLSSFSILLPSLLCSLSTLELSLSVSFSARSLPALFLSATSLF---------EVD 449
Query: 286 IHAMFGAFVFGILVPKDGAFAGALVE 311
+ + GAF+ G LV AF+G + E
Sbjct: 448 LSSTSGAFISGCLVSSSLAFSGCVSE 371
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.140 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,713,180
Number of Sequences: 36976
Number of extensions: 332274
Number of successful extensions: 1978
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1471
Number of HSP's successfully gapped in prelim test: 73
Number of HSP's that attempted gapping in prelim test: 479
Number of HSP's gapped (non-prelim): 1570
length of query: 803
length of database: 9,014,727
effective HSP length: 104
effective length of query: 699
effective length of database: 5,169,223
effective search space: 3613286877
effective search space used: 3613286877
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 62 (28.5 bits)
Medicago: description of AC140913.11