BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140848.2 + phase: 0 /pseudo
(573 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78698 homologue to GP|3746903|gb|AAC64109.1| signal recognitio... 478 e-147
BE941879 homologue to GP|3746903|gb| signal recognition particle... 244 9e-65
TC81509 similar to GP|3746903|gb|AAC64109.1| signal recognition ... 89 6e-33
TC77632 homologue to GP|18086492|gb|AAL57699.1 AT4g30600/F17I23_... 128 5e-30
TC87333 similar to SP|P49972|SR52_LYCES Signal recognition parti... 112 5e-25
TC88278 homologue to SP|P49972|SR52_LYCES Signal recognition par... 99 4e-21
BG649019 similar to PIR|T52612|T52 SRP receptor homolog FtsY pre... 85 7e-17
CB893781 homologue to PIR|C64504|C645 hypothetical protein MJ163... 32 0.66
TC77967 homologue to PIR|T09846|T09846 phosphoenolpyruvate carbo... 30 3.3
TC92621 similar to GP|7107252|gb|AAF36340.1| unknown {Pennisetum... 30 3.3
BG588967 similar to GP|7107246|gb| unknown {Cicer arietinum}, pa... 29 4.3
TC88895 similar to GP|19387172|gb|AAL87122.1 SEC6 {Arabidopsis t... 29 4.3
TC77571 homologue to GP|20804846|dbj|BAB92528. putative rust res... 29 4.3
BG646612 weakly similar to GP|9758140|dbj| disease resistance pr... 28 9.6
CA920643 similar to GP|15010768|gb| AT3g06130/F28L1_7 {Arabidops... 28 9.6
>TC78698 homologue to GP|3746903|gb|AAC64109.1| signal recognition particle
54 kDa subunit precursor {Pisum sativum}, partial (45%)
Length = 1090
Score = 478 bits (1231), Expect(2) = e-147
Identities = 245/248 (98%), Positives = 248/248 (99%)
Frame = +1
Query: 1 MEAVLASRHLSTPLSHHRTTASSSSKLCSSLSYRNSFAKEIWGFVQSKSVTMRRMDMIRP 60
MEAVLASRHLSTPLSHHRTTASSSSKLCSSLSYRNSFAKEIWGFVQSKSVTMRRMDMIRP
Sbjct: 265 MEAVLASRHLSTPLSHHRTTASSSSKLCSSLSYRNSFAKEIWGFVQSKSVTMRRMDMIRP 444
Query: 61 VVVRAEMFGQLTTGLESAWNKLKGEEVLTKDNIVEPMRDIRRALLEADVSLPVVRRFVQS 120
VVVRAEMFGQLTTGLESAWNKLKGEEVLTKDNIVEPMRDIRRALLEADVSLPVVRRFVQS
Sbjct: 445 VVVRAEMFGQLTTGLESAWNKLKGEEVLTKDNIVEPMRDIRRALLEADVSLPVVRRFVQS 624
Query: 121 VTDQAVGVGVTRGVKPDQQLVKIVHDELVQLMGGEVSELTFAKTGPTVILLAGLQGVGKT 180
VTDQAVGVGVTRGVKPDQQLVKIVHDELVQLMGGEVSELTFAKTGPTVILLAGLQGVGKT
Sbjct: 625 VTDQAVGVGVTRGVKPDQQLVKIVHDELVQLMGGEVSELTFAKTGPTVILLAGLQGVGKT 804
Query: 181 TVCAKLANYFKKQGKSCMLVAGDVYRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQG 240
TVCAKLANYFKKQGKSCMLVAGDVYRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQG
Sbjct: 805 TVCAKLANYFKKQGKSCMLVAGDVYRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQG 984
Query: 241 FEEAKKKK 248
FEEA+K++
Sbjct: 985 FEEAQKEE 1008
Score = 62.8 bits (151), Expect(2) = e-147
Identities = 31/34 (91%), Positives = 33/34 (96%)
Frame = +2
Query: 242 EEAKKKKIDVVIVDTAGRLQIDKAMMDELKDVKR 275
++ KKKKIDVVIVDTAGRLQIDKAMMDELKDVKR
Sbjct: 989 KKRKKKKIDVVIVDTAGRLQIDKAMMDELKDVKR 1090
>BE941879 homologue to GP|3746903|gb| signal recognition particle 54 kDa
subunit precursor {Pisum sativum}, partial (23%)
Length = 385
Score = 244 bits (622), Expect = 9e-65
Identities = 126/127 (99%), Positives = 126/127 (99%)
Frame = +3
Query: 312 LTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEK 371
LTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRIL MGDVLSFVEK
Sbjct: 3 LTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILRMGDVLSFVEK 182
Query: 372 AQEVMQQEDAEDLQKKIMSAKFDFNDFLKQTRAVAQMGSVSRVIGMIPGMAKVTPAQIRE 431
AQEVMQQEDAEDLQKKIMSAKFDFNDFLKQTRAVAQMGSVSRVIGMIPGMAKVTPAQIRE
Sbjct: 183 AQEVMQQEDAEDLQKKIMSAKFDFNDFLKQTRAVAQMGSVSRVIGMIPGMAKVTPAQIRE 362
Query: 432 AERNLEI 438
AERNLEI
Sbjct: 363 AERNLEI 383
>TC81509 similar to GP|3746903|gb|AAC64109.1| signal recognition particle 54
kDa subunit precursor {Pisum sativum}, partial (14%)
Length = 636
Score = 89.0 bits (219), Expect(2) = 6e-33
Identities = 42/43 (97%), Positives = 43/43 (99%)
Frame = +1
Query: 493 SGKPTCSSTISNACSDEEPNGCNGRWIDANT**S*GST*NRGK 535
+GKPTCSSTISNACSDEEPNGCNGRWIDANT**S*GST*NRGK
Sbjct: 16 AGKPTCSSTISNACSDEEPNGCNGRWIDANT**S*GST*NRGK 144
Score = 70.5 bits (171), Expect(2) = 6e-33
Identities = 33/34 (97%), Positives = 34/34 (99%)
Frame = +2
Query: 540 QAPPGTARRRKKAESRRLFADSTLRQPPRGFGSK 573
+APPGTARRRKKAESRRLFADSTLRQPPRGFGSK
Sbjct: 143 KAPPGTARRRKKAESRRLFADSTLRQPPRGFGSK 244
>TC77632 homologue to GP|18086492|gb|AAL57699.1 AT4g30600/F17I23_60
{Arabidopsis thaliana}, partial (75%)
Length = 2273
Score = 128 bits (322), Expect = 5e-30
Identities = 82/290 (28%), Positives = 152/290 (52%), Gaps = 18/290 (6%)
Frame = +3
Query: 77 SAWNKLKGEEVLTKDNIVEPMRDIRRALLEADVSLPVVRRFVQSVTDQAVGVGVTRGVKP 136
S + + G+ L K ++ ++ ++ L+ +V+ + + +SV G + +
Sbjct: 1110 SMFQSIAGKANLEKSDLEPALKALKDRLMTKNVAEEIAEKLCESVAASLEGKKLASFTRI 1289
Query: 137 DQQLVKIVHDELVQLMGGEVS-----ELTFAKTG--PTVILLAGLQGVGKTTVCAKLANY 189
+ + D LV+++ S ++ AK P V++ G+ GVGK+T AK+A +
Sbjct: 1290 SSTVQAAMEDALVRILTPRRSIDILRDVHAAKEQRKPYVVVFVGVNGVGKSTNLAKVAYW 1469
Query: 190 FKKQGKSCMLVAGDVYRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQGFEEAKKKKI 249
++ + M+ A D +R A++QL +++ +P++ G + P+ +AK+ +EA +
Sbjct: 1470 LQQHNVNVMMAACDTFRSGAVEQLRTHARRLQIPIFEKGYEKDPAVVAKEAIQEASRNGS 1649
Query: 250 DVVIVDTAGRLQIDKAMMDELKDVKRVLNPTEVLLVVDAMTGQEAAALVTTFNLEIG--- 306
DVV+VDTAGR+Q ++ +M L + + NP VL V +A+ G +A ++ FN ++
Sbjct: 1650 DVVLVDTAGRMQDNEPLMRALSKLIYLNNPDLVLFVGEALVGNDAVDQLSKFNQKLADLS 1829
Query: 307 -------ITGAILTKLDG-DSRGGAALSVKEVSGKPIKLVGRGERMEDLE 348
I G +LTK D D + GAALS+ +SG P+ VG G+ DL+
Sbjct: 1830 TSPTPRLIDGILLTKFDTIDDKVGAALSMVYISGAPVMFVGCGQSYTDLK 1979
>TC87333 similar to SP|P49972|SR52_LYCES Signal recognition particle 54 kDa
protein 2 (SRP54). [Tomato] {Lycopersicon esculentum},
partial (58%)
Length = 1236
Score = 112 bits (279), Expect = 5e-25
Identities = 62/230 (26%), Positives = 117/230 (49%), Gaps = 4/230 (1%)
Frame = +1
Query: 268 DELKDVKRVLNPTEVLLVVDAMTGQEAAALVTTFNLEIGITGAILTKLDGDSRGGAALSV 327
+E++ V P V+ V+D+ GQ A F + + I+TK+DG ++GG ALS
Sbjct: 1 EEMRQVSEATKPDLVIFVMDSSIGQAAFDQAQAFKQSVAVGAVIITKMDGHAKGGGALSA 180
Query: 328 KEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEKAQEVMQQEDAEDLQKK 387
+ P+ +G GE M++ E F R+LG GD+ F+ K EV+ + +L K
Sbjct: 181 VAATKSPVIFIGTGEHMDEFEVFDVKPFVSRLLGQGDLSGFMNKIHEVVPMDQQPELLNK 360
Query: 388 IMSAKFDFNDFLKQTRAVAQMGSVSRVIGMIPGMAK--VTPAQIREAERNLEIMEVIIKA 445
+ F +Q + + +MG +S V M+PG + + + ++++ ++ ++ +
Sbjct: 361 LQEGTFTLRIMYEQFQNILKMGPMSEVFSMLPGFSNELLPKGKDQDSQAKIKRYMTMMDS 540
Query: 446 MTPEERE--KPELLAESPVRRKRVAQDSGKTEQQTDAILSNFEKYTTIMS 493
MT EE + P+L+ ES R R+A+ SG+ ++ ++ +++ I S
Sbjct: 541 MTNEELDSSNPKLMNES--RMMRIARGSGRQVREVMEMMEEYKRLAKIWS 684
>TC88278 homologue to SP|P49972|SR52_LYCES Signal recognition particle 54
kDa protein 2 (SRP54). [Tomato] {Lycopersicon
esculentum}, partial (40%)
Length = 713
Score = 99.0 bits (245), Expect = 4e-21
Identities = 62/208 (29%), Positives = 106/208 (50%)
Frame = +3
Query: 62 VVRAEMFGQLTTGLESAWNKLKGEEVLTKDNIVEPMRDIRRALLEADVSLPVVRRFVQSV 121
+V A++ G +T L+ ++ ++ + + E + +I RALL++DV +VR ++
Sbjct: 102 MVLADLGGSITRALK----QMSNATIIDEKVLNECLNEITRALLQSDVQFKLVRDMQTNI 269
Query: 122 TDQAVGVGVTRGVKPDQQLVKIVHDELVQLMGGEVSELTFAKTGPTVILLAGLQGVGKTT 181
+ G + + + V +EL ++ T K +V++ GLQG GKTT
Sbjct: 270 KKIVNLDDLAAGHNKRRIIQQAVFNELCNILDPGKPAFTPKKGKASVVMFVGLQGSGKTT 449
Query: 182 VCAKLANYFKKQGKSCMLVAGDVYRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQGF 241
C K A Y +K+G LV D +R A DQL + +P Y + + P IA +G
Sbjct: 450 TCTKYAFYHQKKGWKPALVCADTFRAGAFDQLKQNATKAKIPFYGSYMESDPVKIAVEGV 629
Query: 242 EEAKKKKIDVVIVDTAGRLQIDKAMMDE 269
E KK+ D++IVDT+GR + + A+ +E
Sbjct: 630 ERFKKENCDLIIVDTSGRHKQEAALFEE 713
>BG649019 similar to PIR|T52612|T52 SRP receptor homolog FtsY precursor
chloroplast [validated] - Arabidopsis thaliana, partial
(39%)
Length = 788
Score = 85.1 bits (209), Expect = 7e-17
Identities = 56/180 (31%), Positives = 83/180 (46%), Gaps = 32/180 (17%)
Frame = +1
Query: 190 FKKQGKSCMLVAGDVYRPAAIDQLTILGKQVDVPVYTAGTDV-KPSDIAKQGFEEAKKKK 248
F + +L AGD +R AA DQL I ++ + A ++ K S + Q + K+
Sbjct: 178 FSSKFYQILLAAGDTFRAAASDQLEIWAERTGCEIVVAESEKSKASAVLSQAVKRGKELG 357
Query: 249 IDVVIVDTAGRLQIDKAMMDELKDVKRVLNPT---------------------------- 280
D+V+ DT+GRL + +M+EL K+ +
Sbjct: 358 YDIVLCDTSGRLHTNYRLMEELISCKKAVAKVVAGAPNVSFVSFTLDILVLDTARAVAAN 537
Query: 281 ---EVLLVVDAMTGQEAAALVTTFNLEIGITGAILTKLDGDSRGGAALSVKEVSGKPIKL 337
E+LLV+D TG FN +G+TG ILTKLDG +RGG +SV + G P+KL
Sbjct: 538 FIMEILLVLDGTTGLNMLPQAREFNEVVGVTGLILTKLDGSARGGCVVSVVDELGIPVKL 717
>CB893781 homologue to PIR|C64504|C645 hypothetical protein MJ1637 -
Methanococcus jannaschii, partial (2%)
Length = 473
Score = 32.0 bits (71), Expect = 0.66
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = +2
Query: 158 ELTFAKTGPTVILLAGLQGVGKTTVCAKLANYFKKQGKSCMLVA 201
++ + G + ++L GL+G+GKTT+ KL + GKS +L A
Sbjct: 161 KMELLRGGSSTLVLTGLRGLGKTTLATKLCWDEEVNGKS*ILFA 292
>TC77967 homologue to PIR|T09846|T09846 phosphoenolpyruvate carboxylase (EC
4.1.1.31) 1 - upland cotton, partial (79%)
Length = 2660
Score = 29.6 bits (65), Expect = 3.3
Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 7/59 (11%)
Frame = +3
Query: 430 REAERNLEIMEVIIKAMT-------PEEREKPELLAESPVRRKRVAQDSGKTEQQTDAI 481
+E+ER+ ++M+ I K + EER + LL+E +R D KTE+ +D +
Sbjct: 1677 QESERHTDVMDAITKHLQIGSYREWSEERRQEWLLSELSGKRPLFGHDLPKTEEISDVL 1853
>TC92621 similar to GP|7107252|gb|AAF36340.1| unknown {Pennisetum glaucum},
partial (10%)
Length = 827
Score = 29.6 bits (65), Expect = 3.3
Identities = 14/38 (36%), Positives = 23/38 (59%)
Frame = +2
Query: 158 ELTFAKTGPTVILLAGLQGVGKTTVCAKLANYFKKQGK 195
++ + G + +LL GL G+GKTT+ KL + +GK
Sbjct: 497 KMELLRDGRSTLLLTGLGGMGKTTLATKLCLDQQVKGK 610
>BG588967 similar to GP|7107246|gb| unknown {Cicer arietinum}, partial (85%)
Length = 699
Score = 29.3 bits (64), Expect = 4.3
Identities = 31/114 (27%), Positives = 54/114 (47%), Gaps = 5/114 (4%)
Frame = +3
Query: 147 ELVQLMGGEVSELTFAKTGPTVILLAGLQGVGKTTVCAKLAN--YFKKQGKSCMLVAGDV 204
E++QL+ E SE T VI + G G+GKTT+ + N + + KS +L V
Sbjct: 147 EVIQLLNTEPSEET------RVIGICGTGGIGKTTIAKAVYNKIHHHFEAKSFLLNVRQV 308
Query: 205 YRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQG---FEEAKKKKIDVVIVD 255
+ + L +Q+ +Y TD+K + + G +E +K +++VD
Sbjct: 309 WEQD--NGEVSLQQQLLSDIYKT-TDIKKIETVESGKMILQEMLPQKRMLLVVD 461
>TC88895 similar to GP|19387172|gb|AAL87122.1 SEC6 {Arabidopsis thaliana},
partial (52%)
Length = 1551
Score = 29.3 bits (64), Expect = 4.3
Identities = 20/54 (37%), Positives = 29/54 (53%), Gaps = 6/54 (11%)
Frame = +2
Query: 202 GDVYRPAAIDQLTILGKQVDVPVYTAGTDVKPSDIAKQ------GFEEAKKKKI 249
G +Y PAA+D ILG+QV + V TDV I+ F+ A+KK++
Sbjct: 215 GKLYTPAAVDLFRILGEQVQI-VRDNSTDVMLYRISLATIQVMIDFQAAEKKRL 373
>TC77571 homologue to GP|20804846|dbj|BAB92528. putative rust resistance
protein {Oryza sativa (japonica cultivar-group)},
partial (1%)
Length = 954
Score = 29.3 bits (64), Expect = 4.3
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +2
Query: 163 KTGPTVILLAGLQGVGKTTVCAKLANYFKKQGK 195
+ G + +LL GL G+GKTT+ KL + +GK
Sbjct: 656 REGRSTLLLTGLGGMGKTTLATKLCLDDQVKGK 754
>BG646612 weakly similar to GP|9758140|dbj| disease resistance protein-like
{Arabidopsis thaliana}, partial (7%)
Length = 735
Score = 28.1 bits (61), Expect = 9.6
Identities = 11/29 (37%), Positives = 19/29 (64%)
Frame = +2
Query: 158 ELTFAKTGPTVILLAGLQGVGKTTVCAKL 186
++ + G + ++L GL G+GKTT+ KL
Sbjct: 467 KMELIRDGSSTLVLTGLGGLGKTTLATKL 553
>CA920643 similar to GP|15010768|gb| AT3g06130/F28L1_7 {Arabidopsis
thaliana}, partial (15%)
Length = 776
Score = 28.1 bits (61), Expect = 9.6
Identities = 11/28 (39%), Positives = 16/28 (56%)
Frame = -3
Query: 489 TTIMSGKPTCSSTISNACSDEEPNGCNG 516
TT + T ++TI+ C+DE CNG
Sbjct: 492 TTTATSAATTTATINGCCNDESTTCCNG 409
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.135 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,788,167
Number of Sequences: 36976
Number of extensions: 149186
Number of successful extensions: 885
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 883
length of query: 573
length of database: 9,014,727
effective HSP length: 101
effective length of query: 472
effective length of database: 5,280,151
effective search space: 2492231272
effective search space used: 2492231272
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC140848.2