BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140774.9 - phase: 0 /pseudo
(174 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87648 similar to GP|17529294|gb|AAL38874.1 unknown protein {Ar... 40 6e-04
TC76371 weakly similar to GP|23304737|emb|CAC87937. PDI-like pro... 36 0.009
TC76345 similar to GP|3928152|emb|CAA10290.1 ribulose 1 5-bispho... 36 0.009
TC78691 similar to GP|17380978|gb|AAL36301.1 unknown protein {Ar... 35 0.020
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 30 0.49
BQ150359 30 0.64
TC79620 weakly similar to GP|6648964|gb|AAF21310.1| seed maturat... 29 1.1
CB893991 similar to GP|21553920|gb| unknown {Arabidopsis thalian... 28 1.4
BG588872 weakly similar to GP|21592660|gb phloem-specific lectin... 28 1.9
TC77558 similar to PIR|T10678|T10678 hypothetical protein F3L17.... 28 1.9
BF642588 similar to GP|17979131|g unknown protein {Arabidopsis t... 28 2.4
TC80881 similar to PIR|T11622|T11622 extensin class 1 precursor ... 27 3.2
BF519064 similar to PIR|S57483|S574 glycin-rich protein - cowpea... 27 3.2
BI262892 27 3.2
TC79445 similar to GP|15215626|gb|AAK91358.1 AT4g01560/F11O4_6 {... 27 4.2
BQ150069 27 4.2
TC92783 similar to PIR|T49020|T49020 hypothetical protein F3C22.... 27 4.2
BQ143960 weakly similar to SP|P21997|SSGP_ Sulfated surface glyc... 27 5.4
BQ123351 26 7.1
TC85881 similar to PIR|T00809|T00809 probable esterase D [import... 26 9.3
>TC87648 similar to GP|17529294|gb|AAL38874.1 unknown protein {Arabidopsis
thaliana}, partial (33%)
Length = 990
Score = 39.7 bits (91), Expect = 6e-04
Identities = 17/51 (33%), Positives = 32/51 (62%)
Frame = +1
Query: 65 EEIIHFSHPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFCAL 115
E++ H +H +H L ++ +++ C GCK+ G ++C +C++ LH CAL
Sbjct: 583 EKVTHETH-EHEL-VLSRRNVYCCDGCKDEGDTWSYLCAECDFDLHPNCAL 729
>TC76371 weakly similar to GP|23304737|emb|CAC87937. PDI-like protein {Quercus
suber}, partial (21%)
Length = 1710
Score = 35.8 bits (81), Expect = 0.009
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = +3
Query: 67 IIHFSHPQHPLSMVDLPD---LFICVGCKEYGSGKRFVCQQCNYQLHDFC 113
++H H L++V + FIC C E GS + C QC Y++H C
Sbjct: 1200 LVHHEGHHHGLNLVSDGNGGGPFICCVCDEQGSNWAYQCLQCGYEVHPKC 1349
>TC76345 similar to GP|3928152|emb|CAA10290.1 ribulose 1 5-bisphosphate
carboxylase small subunit {Cicer arietinum}, partial
(98%)
Length = 1243
Score = 35.8 bits (81), Expect = 0.009
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = +1
Query: 67 IIHFSHPQHPLSMVDLPD---LFICVGCKEYGSGKRFVCQQCNYQLHDFC 113
++H H L++V + FIC C E GS + C QC Y++H C
Sbjct: 952 LVHHEGHHHGLNLVSDGNGGGPFICCVCDEQGSNWAYQCLQCGYEVHPKC 1101
>TC78691 similar to GP|17380978|gb|AAL36301.1 unknown protein {Arabidopsis
thaliana}, partial (38%)
Length = 815
Score = 34.7 bits (78), Expect = 0.020
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 77 LSMVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFC 113
L +D+ ++C CK+ G F C C+Y LH C
Sbjct: 375 LLKLDMAKAYVCDSCKKQGKFWTFSCDVCDYDLHPSC 485
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 30.0 bits (66), Expect = 0.49
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +3
Query: 21 SPTKEYQITAPSHDDQYYSKVTTIVTKKSPPPIEFPTSP 59
+P K++ T P+H + ++ T T ++ PP E PT P
Sbjct: 201 NPKKQHTPTTPTHPPRPHTPPTPPPTTQTTPPREAPTDP 317
>BQ150359
Length = 413
Score = 29.6 bits (65), Expect = 0.64
Identities = 15/43 (34%), Positives = 25/43 (57%)
Frame = +3
Query: 18 LEKSPTKEYQITAPSHDDQYYSKVTTIVTKKSPPPIEFPTSPQ 60
L K+P + ++ +P + +S + T+K PPP EFP SP+
Sbjct: 36 LGKTPGR*PKLNSPLQQNSPFSPGLALKTQKRPPP-EFPLSPK 161
>TC79620 weakly similar to GP|6648964|gb|AAF21310.1| seed maturation protein
PM24 {Glycine max}, partial (62%)
Length = 696
Score = 28.9 bits (63), Expect = 1.1
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = -2
Query: 106 NYQLHDFCALAPPALKAHPFHSQH-AMLFHSKPDDKIQMRRLWQTNKRVYIPMHCMRFPN 164
+Y L F +APP + FHS H A+ FH + W+ + ++ H P
Sbjct: 362 SYSLVHFDLIAPPHHRQQHFHSYHYALQFHLSHFSLPPI--AWKQRRHPFVSSHASESPL 189
Query: 165 ASMLCH 170
++ L H
Sbjct: 188 SASLPH 171
>CB893991 similar to GP|21553920|gb| unknown {Arabidopsis thaliana}, partial
(21%)
Length = 610
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/52 (30%), Positives = 26/52 (49%)
Frame = -3
Query: 29 TAPSHDDQYYSKVTTIVTKKSPPPIEFPTSPQLIFGEEIIHFSHPQHPLSMV 80
T+PS Q +S ++ +P P PTS L+ F+HP+ PL ++
Sbjct: 350 TSPSLTLQSFSTSSSQTITLAPTP---PTSTSLLIHRRHRRFTHPKQPLPLI 204
>BG588872 weakly similar to GP|21592660|gb phloem-specific lectin PP2-like
protein {Arabidopsis thaliana}, partial (55%)
Length = 655
Score = 28.1 bits (61), Expect = 1.9
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 40 KVTTIVTKKSPPPIEFPTSPQLIFGEEIIHFSHP 73
KVT++ K PP F +P ++FG+ I F P
Sbjct: 506 KVTSLNHKHDPPIFTFRYAPYIVFGDNISLFILP 405
>TC77558 similar to PIR|T10678|T10678 hypothetical protein F3L17.50 -
Arabidopsis thaliana, partial (53%)
Length = 1889
Score = 28.1 bits (61), Expect = 1.9
Identities = 10/35 (28%), Positives = 21/35 (59%)
Frame = +1
Query: 71 SHPQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQC 105
S P + + ++PD +C+G + + S + VC++C
Sbjct: 1321 SGPHYQVYKYEVPDSTLCIGRRVWFSSCKSVCKEC 1425
>BF642588 similar to GP|17979131|g unknown protein {Arabidopsis thaliana},
partial (14%)
Length = 589
Score = 27.7 bits (60), Expect = 2.4
Identities = 16/65 (24%), Positives = 25/65 (37%), Gaps = 11/65 (16%)
Frame = +2
Query: 73 PQHPLSMVDLPDLFICVGCKEYGSGKRFVCQQCN-----------YQLHDFCALAPPALK 121
P H + +P ++C+ C +R C QCN L + +L L+
Sbjct: 371 PGHGHKSITIPSDWMCIICSYINFARRTSCYQCNEPRTDDAPAADISLSNSTSLXKKGLE 550
Query: 122 AHPFH 126
A P H
Sbjct: 551 AGPTH 565
>TC80881 similar to PIR|T11622|T11622 extensin class 1 precursor - cowpea,
partial (34%)
Length = 597
Score = 27.3 bits (59), Expect = 3.2
Identities = 17/41 (41%), Positives = 17/41 (41%), Gaps = 3/41 (7%)
Frame = +2
Query: 22 PTKEY-QITAPSHDDQ--YYSKVTTIVTKKSPPPIEFPTSP 59
P Y Q T PSH Q YY K K PPP P P
Sbjct: 152 PNNNYPQPTPPSHGQQPPYYFKSPPYYYKSPPPPSPSPPPP 274
>BF519064 similar to PIR|S57483|S574 glycin-rich protein - cowpea (fragment),
partial (7%)
Length = 452
Score = 27.3 bits (59), Expect = 3.2
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 9 VEKKTNTFLLEKSPTKEYQITAPSHDDQYYSKVTTIVTKKSPPPI 53
++KK N FL K T ++ +S+ T + KK+PPP+
Sbjct: 223 LKKKKNFFLFIKKKTPLWETLLGGRGFLIFSRGKTPLGKKTPPPL 357
>BI262892
Length = 263
Score = 27.3 bits (59), Expect = 3.2
Identities = 18/75 (24%), Positives = 31/75 (41%), Gaps = 9/75 (12%)
Frame = -3
Query: 108 QLHDFCALAPPALKAHPFH----SQHAMLFHSKPDDKIQMRRLWQTN-----KRVYIPMH 158
+LHD+ P + +P+H H +S P + + + + +Q N K H
Sbjct: 243 RLHDYSLCCPSSSSYYPYHVPSLPHHQPETNSLPSELVSVLQHFQLNNDHSDKLASKSHH 64
Query: 159 CMRFPNASMLCHAQH 173
PN+S+ H H
Sbjct: 63 SQH*PNSSLSLHLLH 19
>TC79445 similar to GP|15215626|gb|AAK91358.1 AT4g01560/F11O4_6 {Arabidopsis
thaliana}, partial (86%)
Length = 1443
Score = 26.9 bits (58), Expect = 4.2
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -1
Query: 56 PTSPQLIFGEEIIHFSHPQH 75
P QL +G+E+ H HP+H
Sbjct: 885 PEDGQLFYGQELSHSCHPRH 826
>BQ150069
Length = 378
Score = 26.9 bits (58), Expect = 4.2
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +2
Query: 44 IVTKKSPPPIEFPTSPQLIFGEEIIHFSHPQHP 76
++TKK PP FP SP+ + ++F + P
Sbjct: 122 LITKKRAPPPIFPFSPKKLCAHPPLYFGEKKGP 220
>TC92783 similar to PIR|T49020|T49020 hypothetical protein F3C22.70 -
Arabidopsis thaliana (fragment), partial (7%)
Length = 679
Score = 26.9 bits (58), Expect = 4.2
Identities = 20/79 (25%), Positives = 34/79 (42%), Gaps = 4/79 (5%)
Frame = +3
Query: 6 QKQVEKKTNTFLLEKSPTK----EYQITAPSHDDQYYSKVTTIVTKKSPPPIEFPTSPQL 61
QK + +TN L+K PT+ Y + P+ + ++ + ++ SP SP L
Sbjct: 60 QKTTKNRTNQHRLDKHPTRLTPLPYTLLPPNQNHRHNNP-----SRLSPLAPSLGESPSL 224
Query: 62 IFGEEIIHFSHPQHPLSMV 80
F +I H Q L +
Sbjct: 225 RFLSQIQQTHHQQQRLQKI 281
>BQ143960 weakly similar to SP|P21997|SSGP_ Sulfated surface glycoprotein
185 (SSG 185). {Volvox carteri}, partial (13%)
Length = 1023
Score = 26.6 bits (57), Expect = 5.4
Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 6/33 (18%)
Frame = +2
Query: 50 PPPIEFPT------SPQLIFGEEIIHFSHPQHP 76
PPP FP+ SP LI + HF P HP
Sbjct: 20 PPPSPFPSSPPPPPSPPLISPPFLSHFLFPPHP 118
>BQ123351
Length = 725
Score = 26.2 bits (56), Expect = 7.1
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 11 KKTNTFLLEKSPTKEYQITAPSHD 34
KK NTF ++P +Y ++ P+HD
Sbjct: 115 KKWNTFPPTEAPIFDYPVSIPTHD 186
>TC85881 similar to PIR|T00809|T00809 probable esterase D [imported] -
Arabidopsis thaliana, partial (95%)
Length = 1091
Score = 25.8 bits (55), Expect = 9.3
Identities = 12/42 (28%), Positives = 18/42 (42%)
Frame = -3
Query: 129 HAMLFHSKPDDKIQMRRLWQTNKRVYIPMHCMRFPNASMLCH 170
HA + H PD + WQ ++ +P R P + CH
Sbjct: 750 HAEICHLPPDQS----KCWQIHQETLLPSE*HRNPPIQIYCH 637
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.136 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,294,775
Number of Sequences: 36976
Number of extensions: 129991
Number of successful extensions: 994
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 974
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 994
length of query: 174
length of database: 9,014,727
effective HSP length: 89
effective length of query: 85
effective length of database: 5,723,863
effective search space: 486528355
effective search space used: 486528355
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC140774.9