BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140773.5 - phase: 0 /pseudo
(194 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89992 auxin influx carrier protein [Medicago truncatula] 30 0.45
TC81008 weakly similar to GP|8778855|gb|AAF79854.1| T7N9.11 {Ara... 30 0.45
TC90247 weakly similar to GP|21592898|gb|AAM64848.1 unknown {Ara... 30 0.59
TC92940 weakly similar to PIR|T06410|T06410 GTP cyclohydrolase I... 30 0.77
TC88436 similar to GP|9293867|dbj|BAB01770.1 DEAD-Box RNA helica... 30 0.77
TC88482 auxin influx carrier protein [Medicago truncatula] 29 1.0
BQ147403 weakly similar to GP|20197205|gb| expressed protein {Ar... 29 1.0
TC80455 similar to GP|13374867|emb|CAC34501. putative protein {A... 29 1.0
AW694142 similar to GP|19703145|gb hypothetical protein {Dictyos... 28 2.2
TC90244 similar to GP|15450749|gb|AAK96646.1 At2g39750/T5I7.5 {A... 28 2.9
TC86757 auxin influx carrier protein [Medicago truncatula] 27 3.8
TC87460 similar to GP|15983356|gb|AAL11546.1 unknown protein {Ar... 27 3.8
TC86662 weakly similar to GP|4756646|emb|CAB42250.1 unnamed prot... 27 3.8
TC83882 similar to GP|20197942|gb|AAD31580.2 putative farnesylat... 27 3.8
TC85220 weakly similar to PIR|S33520|S33520 Lea protein - soybea... 27 3.8
BE321102 similar to GP|15983356|gb|A unknown protein {Arabidopsi... 27 3.8
TC88600 weakly similar to PIR|H96603|H96603 unknown protein F14G... 27 5.0
TC87643 similar to PIR|C71405|C71405 probable casein kinase I - ... 27 5.0
TC86288 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max},... 27 5.0
TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8.... 27 6.5
>TC89992 auxin influx carrier protein [Medicago truncatula]
Length = 1676
Score = 30.4 bits (67), Expect = 0.45
Identities = 13/45 (28%), Positives = 25/45 (54%), Gaps = 3/45 (6%)
Frame = +2
Query: 146 YDLSFLKSKFRTEPKIEN---NEKYLDWLDKVEKIKGPFWKEMGV 187
Y +S L ++R+ + EN + W + +E + GP+WK +G+
Sbjct: 302 YLISILYVEYRSRKEKENVSFKNHVIQWFEVLEGLLGPYWKAIGL 436
>TC81008 weakly similar to GP|8778855|gb|AAF79854.1| T7N9.11 {Arabidopsis
thaliana}, partial (19%)
Length = 760
Score = 30.4 bits (67), Expect = 0.45
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 4/64 (6%)
Frame = -1
Query: 4 ICPISKAIYMTLSHIF----TLTSFLLSLLLLFSPSPSSTLRFQNIVHNSTKNFEIFMAR 59
+ PI++A + L + LTSF + L LF P+PS + ++ HN+ E+ +
Sbjct: 481 VFPIAEASLLPLPCLLRGASALTSFDIDTLSLFLPTPSLHIARGSVAHNT*TFLELTVTP 302
Query: 60 VEGS 63
EGS
Sbjct: 301 NEGS 290
>TC90247 weakly similar to GP|21592898|gb|AAM64848.1 unknown {Arabidopsis
thaliana}, partial (60%)
Length = 1041
Score = 30.0 bits (66), Expect = 0.59
Identities = 15/42 (35%), Positives = 25/42 (58%)
Frame = +2
Query: 2 IDICPISKAIYMTLSHIFTLTSFLLSLLLLFSPSPSSTLRFQ 43
I + +S A+ +++ L SF+ LLLLFS PS +L+ +
Sbjct: 176 IHVLHLSMALSLSVLAFLLLLSFITLLLLLFSSGPSPSLKLK 301
>TC92940 weakly similar to PIR|T06410|T06410 GTP cyclohydrolase II (EC
3.5.4.25) / 3 4-dihydroxy-2-butanone 4-phosphate
synthase (EC 5.4.99.-), partial (17%)
Length = 643
Score = 29.6 bits (65), Expect = 0.77
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = -1
Query: 96 IWKSQVIISCPNSSHEY 112
+WK +++ CPN SH Y
Sbjct: 466 VWKQNLLLDCPNQSHRY 416
>TC88436 similar to GP|9293867|dbj|BAB01770.1 DEAD-Box RNA helicase-like
protein {Arabidopsis thaliana}, partial (38%)
Length = 1024
Score = 29.6 bits (65), Expect = 0.77
Identities = 19/90 (21%), Positives = 41/90 (45%), Gaps = 11/90 (12%)
Frame = -2
Query: 21 LTSFLLSLLLLFSP-----------SPSSTLRFQNIVHNSTKNFEIFMARVEGSSSQPTK 69
L F L+LL FS SP +++ +++H+ + F + GS++ T
Sbjct: 594 LVEFFLNLLRKFSSRRQNQSQRILSSPMFSIKLNDLIHDGYPKSKSFSSSCSGSTNHITT 415
Query: 70 QHEPNVNLPVNTQFMEEPDVKEAEDKIWKS 99
H + P+N + + + + ++ D I ++
Sbjct: 414 LHSRFQHSPLNGEKLGDSFLLQSRDNILRN 325
>TC88482 auxin influx carrier protein [Medicago truncatula]
Length = 2261
Score = 29.3 bits (64), Expect = 1.0
Identities = 12/45 (26%), Positives = 25/45 (54%), Gaps = 3/45 (6%)
Frame = +1
Query: 146 YDLSFLKSKFRTEPKIEN---NEKYLDWLDKVEKIKGPFWKEMGV 187
Y +S L ++R+ + EN + W + ++ + GP+WK +G+
Sbjct: 640 YLISVLYVEYRSRKEKENVNFKNHVIQWFEVLDGLLGPYWKALGL 774
>BQ147403 weakly similar to GP|20197205|gb| expressed protein {Arabidopsis
thaliana}, partial (14%)
Length = 585
Score = 29.3 bits (64), Expect = 1.0
Identities = 22/70 (31%), Positives = 35/70 (49%)
Frame = +1
Query: 22 TSFLLSLLLLFSPSPSSTLRFQNIVHNSTKNFEIFMARVEGSSSQPTKQHEPNVNLPVNT 81
T FLLSLLLL S + TL + VH + + ++F + + K+ P+ P +
Sbjct: 10 TLFLLSLLLLISLNSHFTLAGKRKVHITDELDDVFDDEEDDDWKEWGKKPSPSF-APADL 186
Query: 82 QFMEEPDVKE 91
M+E +KE
Sbjct: 187TKMDESKIKE 216
>TC80455 similar to GP|13374867|emb|CAC34501. putative protein {Arabidopsis
thaliana}, partial (83%)
Length = 658
Score = 29.3 bits (64), Expect = 1.0
Identities = 23/106 (21%), Positives = 49/106 (45%), Gaps = 3/106 (2%)
Frame = +1
Query: 63 SSSQPTKQHEPNVNLPVNTQFMEEPDVKEAEDKIWKSQVIISCPNSSHEYLGPLSDESDD 122
SS K+ ++L V+ + P KEA + K+Q ++ PN L ++ ++
Sbjct: 166 SSIDEVKKQYRKISLMVHPDKCKHPQAKEAFGALSKAQQLLLDPNERDYLLSQVNSAKEE 345
Query: 123 --QKKLDAVFPWNEAQLPLIFSKGPYDLSFLKS-KFRTEPKIENNE 165
K+ + +++ + +G YD + +S +F+ E K++ E
Sbjct: 346 LRVKRKKHLKKDTASKMKSLVDEGKYDKQYEQSEEFKQELKLKVRE 483
>AW694142 similar to GP|19703145|gb hypothetical protein {Dictyostelium
discoideum}, partial (1%)
Length = 450
Score = 28.1 bits (61), Expect = 2.2
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -1
Query: 144 GPYDLSFLKSKFRTEPKIENNEKYLDWL 171
G DL +L K E K++NN+K + W+
Sbjct: 126 GDCDLHYLDQKKAWEEKMQNNDKLVSWI 43
>TC90244 similar to GP|15450749|gb|AAK96646.1 At2g39750/T5I7.5 {Arabidopsis
thaliana}, partial (49%)
Length = 1319
Score = 27.7 bits (60), Expect = 2.9
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -1
Query: 9 KAIYMTLSHIFTLTSFLLSLLLLFSPSPSSTLRFQNIVHNSTKN 52
+A+Y T+ + + ++LLSL + F P SST R + N T +
Sbjct: 491 RAVYKTVYRLLEI*TYLLSLEIQFWPPLSSTRRV*GTLLNHTSS 360
>TC86757 auxin influx carrier protein [Medicago truncatula]
Length = 1590
Score = 27.3 bits (59), Expect = 3.8
Identities = 11/46 (23%), Positives = 24/46 (51%), Gaps = 3/46 (6%)
Frame = +1
Query: 146 YDLSFLKSKFRTEP---KIENNEKYLDWLDKVEKIKGPFWKEMGVF 188
Y +S L ++RT K++ + W + ++ + G W+ +G+F
Sbjct: 361 YIISVLYVEYRTRKEREKVDFRNHVIQWFEVLDGLLGKHWRNLGLF 498
>TC87460 similar to GP|15983356|gb|AAL11546.1 unknown protein {Arabidopsis
thaliana}, partial (95%)
Length = 584
Score = 27.3 bits (59), Expect = 3.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 148 LSFLKSKFRTEPKIENNEKYLDWLDKVEKIKGPF 181
+S L+ FR KI +KY + K +KGPF
Sbjct: 120 MSDLEPPFRPREKIIEKQKYFQHIPKHTYLKGPF 221
>TC86662 weakly similar to GP|4756646|emb|CAB42250.1 unnamed protein product
{unidentified}, partial (76%)
Length = 1161
Score = 27.3 bits (59), Expect = 3.8
Identities = 22/79 (27%), Positives = 38/79 (47%), Gaps = 2/79 (2%)
Frame = +1
Query: 10 AIYMTLSHIFTLTSFLLSLLLL--FSPSPSSTLRFQNIVHNSTKNFEIFMARVEGSSSQP 67
+++++LS F + SFLLSLLL F + S T RF + ++ F + + SS
Sbjct: 52 SLFVSLSSSFQVFSFLLSLLLCLSFFLALSHTQRFAH-TYDKNGTFHSLLCLIYPSSPVS 228
Query: 68 TKQHEPNVNLPVNTQFMEE 86
H + ++ F E+
Sbjct: 229 LPNHHSQLRRECSSCF*EQ 285
>TC83882 similar to GP|20197942|gb|AAD31580.2 putative farnesylated protein
{Arabidopsis thaliana}, partial (4%)
Length = 670
Score = 27.3 bits (59), Expect = 3.8
Identities = 17/50 (34%), Positives = 26/50 (52%)
Frame = -1
Query: 22 TSFLLSLLLLFSPSPSSTLRFQNIVHNSTKNFEIFMARVEGSSSQPTKQH 71
TS S+ L SPSP S+ QN+ + TK + F V+ + P +Q+
Sbjct: 499 TSPSSSIRALSSPSPPSSESLQNLQYPETKIPQFFNPLVKVPNFDPPRQN 350
>TC85220 weakly similar to PIR|S33520|S33520 Lea protein - soybean, partial
(69%)
Length = 1880
Score = 27.3 bits (59), Expect = 3.8
Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 5/60 (8%)
Frame = -1
Query: 2 IDICPISKAIYMTL-----SHIFTLTSFLLSLLLLFSPSPSSTLRFQNIVHNSTKNFEIF 56
+D+C +S A + L ++ L+ +LSL L P PS + VH S +F F
Sbjct: 416 LDLCAVSSA*CLVLCAASEAYSEALSVVVLSLSLSLKPRPSVIFSLAHSVHVSAASFPSF 237
>BE321102 similar to GP|15983356|gb|A unknown protein {Arabidopsis thaliana},
partial (95%)
Length = 287
Score = 27.3 bits (59), Expect = 3.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 148 LSFLKSKFRTEPKIENNEKYLDWLDKVEKIKGPF 181
+S L+ FR KI +KY + K +KGPF
Sbjct: 66 MSDLEPPFRPREKIIEKQKYFQHIPKHTYLKGPF 167
>TC88600 weakly similar to PIR|H96603|H96603 unknown protein F14G9.16
[imported] - Arabidopsis thaliana, partial (11%)
Length = 884
Score = 26.9 bits (58), Expect = 5.0
Identities = 16/68 (23%), Positives = 35/68 (50%), Gaps = 2/68 (2%)
Frame = +1
Query: 12 YMTLSHIFTLTSFLLSLLLLFSPSPSSTLRFQNIV--HNSTKNFEIFMARVEGSSSQPTK 69
Y L+H+ T L S+++LF+P+ + T +++ H S ++ + R+ S T
Sbjct: 46 YKILTHVQTKPP*LNSIIILFAPNSN*TTNNTSMILSH*SISTLQLLLLRIMFLSLLSTS 225
Query: 70 QHEPNVNL 77
+ P++ +
Sbjct: 226 TNLPHLKI 249
>TC87643 similar to PIR|C71405|C71405 probable casein kinase I - Arabidopsis
thaliana, partial (71%)
Length = 2349
Score = 26.9 bits (58), Expect = 5.0
Identities = 22/75 (29%), Positives = 37/75 (49%), Gaps = 1/75 (1%)
Frame = +1
Query: 41 RFQNIVHNSTKNFEIFMARVEGS-SSQPTKQHEPNVNLPVNTQFMEEPDVKEAEDKIWKS 99
R ++ S KN EIF R+ G+ + T+ + + V+T +E + +AE IW
Sbjct: 1003 RSTSLTMASPKNSEIF--RLIGTYHTGKTRTLQARLGTRVSTLILE---LNKAEGMIWNL 1167
Query: 100 QVIISCPNSSHEYLG 114
V+ SC + +LG
Sbjct: 1168 LVMFSCTS*EEVFLG 1212
>TC86288 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max}, partial
(84%)
Length = 2900
Score = 26.9 bits (58), Expect = 5.0
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +2
Query: 17 HIFTLTSFLLSLLLLFSPSPS 37
H F+L F L L+ FSPSPS
Sbjct: 17 HFFSLPFFFLPYLIGFSPSPS 79
>TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8.1.6)
(Biotin synthetase). [Mouse-ear cress] {Arabidopsis
thaliana}, partial (87%)
Length = 1555
Score = 26.6 bits (57), Expect = 6.5
Identities = 13/47 (27%), Positives = 27/47 (56%), Gaps = 6/47 (12%)
Frame = -1
Query: 59 RVEGSSSQP------TKQHEPNVNLPVNTQFMEEPDVKEAEDKIWKS 99
R+EG+ + P +++ E V LP + + + EP+ + ED+ W++
Sbjct: 574 RIEGNKNNPHYTPQFSQREEYTVALP*SYEHVYEPEPRGREDQEWEN 434
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,459,327
Number of Sequences: 36976
Number of extensions: 89998
Number of successful extensions: 717
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 714
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of query: 194
length of database: 9,014,727
effective HSP length: 91
effective length of query: 103
effective length of database: 5,649,911
effective search space: 581940833
effective search space used: 581940833
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC140773.5