Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140721.8 + phase: 0 /pseudo
         (59 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC87484 homologue to GP|6689036|emb|CAB65370.1 germin-like prote...    77  1e-15
TC85455 similar to GP|6689036|emb|CAB65370.1 germin-like protein...    61  8e-11
TC87485 homologue to GP|6689036|emb|CAB65370.1 germin-like prote...    52  4e-08
TC78488 similar to GP|6689036|emb|CAB65370.1 germin-like protein...    49  4e-07
AJ498555 weakly similar to SP|Q9FMA8|GL1B Germin-like protein su...    36  0.003
AL382776 weakly similar to SP|P92996|GL1K_ Germin-like protein s...    31  0.11
TC81825 weakly similar to SP|Q9FIC8|GL1G_ARATH Germin-like prote...    30  0.25
BQ140773                                                               29  0.42
TC79592 similar to GP|1934730|gb|AAB51752.1| germin-like protein...    29  0.42
TC88864 similar to GP|1934730|gb|AAB51752.1| germin-like protein...    28  0.71
TC79767 similar to SP|Q9SR72|GL32_ARATH Germin-like protein subf...    26  3.5
AW688621 weakly similar to SP|P92998|GL11 Germin-like protein su...    25  4.6
TC86586 weakly similar to GP|15528795|dbj|BAB64690. germin(oxala...    25  4.6
TC93604 weakly similar to SP|Q9SR72|GL32_ARATH Germin-like prote...    25  6.0
AW586190 similar to SP|Q9S8P4|RHRE_ Rhicadhesin receptor precurs...    25  7.9
BE319693                                                               25  7.9

>TC87484 homologue to GP|6689036|emb|CAB65370.1 germin-like protein {Pisum
           sativum}, complete
          Length = 950

 Score = 77.4 bits (189), Expect = 1e-15
 Identities = 37/39 (94%), Positives = 38/39 (96%)
 Frame = +2

Query: 1   MKILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDG 39
           MKILY LVSILALASS+AFAYDPSPLQDFCVAIKDPKDG
Sbjct: 71  MKILYFLVSILALASSLAFAYDPSPLQDFCVAIKDPKDG 187



 Score = 37.7 bits (86), Expect = 9e-04
 Identities = 17/19 (89%), Positives = 18/19 (94%)
 Frame = +3

Query: 40  DYSDLTHLVYLWLALILPL 58
           +YSDLTHLVYLWLALIL L
Sbjct: 315 NYSDLTHLVYLWLALILCL 371


>TC85455 similar to GP|6689036|emb|CAB65370.1 germin-like protein {Pisum
          sativum}, partial (97%)
          Length = 929

 Score = 61.2 bits (147), Expect = 8e-11
 Identities = 27/39 (69%), Positives = 32/39 (81%)
 Frame = +1

Query: 1  MKILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDG 39
          M + Y +V++LALASS+ FAYDPSPLQDFCVAI D K G
Sbjct: 52 MGVFYFIVALLALASSVGFAYDPSPLQDFCVAINDTKTG 168



 Score = 29.3 bits (64), Expect = 0.32
 Identities = 13/16 (81%), Positives = 13/16 (81%)
 Frame = +2

Query: 41  YSDLTHLVYLWLALIL 56
           Y D THLVYLWLA IL
Sbjct: 302 Y*DSTHLVYLWLASIL 349


>TC87485 homologue to GP|6689036|emb|CAB65370.1 germin-like protein {Pisum
          sativum}, complete
          Length = 888

 Score = 52.4 bits (124), Expect = 4e-08
 Identities = 22/23 (95%), Positives = 23/23 (99%)
 Frame = +2

Query: 17 IAFAYDPSPLQDFCVAIKDPKDG 39
          +AFAYDPSPLQDFCVAIKDPKDG
Sbjct: 2  LAFAYDPSPLQDFCVAIKDPKDG 70



 Score = 37.4 bits (85), Expect = 0.001
 Identities = 17/20 (85%), Positives = 18/20 (90%)
 Frame = +3

Query: 40  DYSDLTHLVYLWLALILPLR 59
           +YSDLTHL YLWLALIL LR
Sbjct: 198 NYSDLTHLGYLWLALILRLR 257


>TC78488 similar to GP|6689036|emb|CAB65370.1 germin-like protein {Pisum
          sativum}, partial (98%)
          Length = 882

 Score = 48.9 bits (115), Expect = 4e-07
 Identities = 23/39 (58%), Positives = 27/39 (68%)
 Frame = +2

Query: 1  MKILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDG 39
          MK   L V+     +S AFA+DPSPLQDFCVAI D K+G
Sbjct: 32 MKTSILFVTAFLALASCAFAFDPSPLQDFCVAINDTKNG 148


>AJ498555 weakly similar to SP|Q9FMA8|GL1B Germin-like protein subfamily 1
          member 11 precursor. [Mouse-ear cress] {Arabidopsis
          thaliana}, partial (65%)
          Length = 499

 Score = 36.2 bits (82), Expect = 0.003
 Identities = 17/25 (68%), Positives = 18/25 (72%)
 Frame = +2

Query: 15 SSIAFAYDPSPLQDFCVAIKDPKDG 39
          +S AFA D SPLQDFCVA  D K G
Sbjct: 5  TSFAFALDLSPLQDFCVATNDIKTG 79


>AL382776 weakly similar to SP|P92996|GL1K_ Germin-like protein subfamily 1
          member 20 precursor (GLP2a copy 2) (Germin type 2)
          (GLP2b), partial (39%)
          Length = 444

 Score = 30.8 bits (68), Expect = 0.11
 Identities = 19/46 (41%), Positives = 24/46 (51%), Gaps = 9/46 (19%)
 Frame = +3

Query: 1  MKILYLLVSILALASSIAFAYDPSPLQDFCVA---------IKDPK 37
          M  L L++ +LAL+    FA D + LQDFCV           KDPK
Sbjct: 39 MANLSLMLPLLALSLLAVFAADATTLQDFCVTNPIGQGNSLCKDPK 176


>TC81825 weakly similar to SP|Q9FIC8|GL1G_ARATH Germin-like protein
          subfamily 1 member 16 precursor. [Mouse-ear cress]
          {Arabidopsis thaliana}, partial (76%)
          Length = 1042

 Score = 29.6 bits (65), Expect = 0.25
 Identities = 14/28 (50%), Positives = 19/28 (67%)
 Frame = +1

Query: 4  LYLLVSILALASSIAFAYDPSPLQDFCV 31
          L L++ +LAL+    FA D + LQDFCV
Sbjct: 4  LSLMLPLLALSLLAVFAADATTLQDFCV 87


>BQ140773 
          Length = 653

 Score = 28.9 bits (63), Expect = 0.42
 Identities = 10/18 (55%), Positives = 14/18 (77%)
 Frame = +3

Query: 23  PSPLQDFCVAIKDPKDGD 40
           PSP++ FCVA+ +P D D
Sbjct: 363 PSPIRPFCVALAEPNDRD 416


>TC79592 similar to GP|1934730|gb|AAB51752.1| germin-like protein
           {Arabidopsis thaliana}, partial (96%)
          Length = 947

 Score = 28.9 bits (63), Expect = 0.42
 Identities = 13/29 (44%), Positives = 17/29 (57%)
 Frame = +3

Query: 5   YLLVSILALASSIAFAYDPSPLQDFCVAI 33
           + ++ IL  A S   A DP  LQD CVA+
Sbjct: 111 WTILVILCCAISFTIASDPDTLQDLCVAL 197


>TC88864 similar to GP|1934730|gb|AAB51752.1| germin-like protein
           {Arabidopsis thaliana}, partial (91%)
          Length = 926

 Score = 28.1 bits (61), Expect = 0.71
 Identities = 13/27 (48%), Positives = 17/27 (62%)
 Frame = +1

Query: 6   LLVSILALASSIAFAYDPSPLQDFCVA 32
           L +  LAL  + +F+ DP  LQD CVA
Sbjct: 85  LFLGTLALLLATSFSSDPDYLQDLCVA 165


>TC79767 similar to SP|Q9SR72|GL32_ARATH Germin-like protein subfamily 3
          member 2 precursor. [Mouse-ear cress] {Arabidopsis
          thaliana}, partial (84%)
          Length = 965

 Score = 25.8 bits (55), Expect = 3.5
 Identities = 8/13 (61%), Positives = 11/13 (84%)
 Frame = +3

Query: 19 FAYDPSPLQDFCV 31
          +A DP P+QDFC+
Sbjct: 15 YASDPDPVQDFCI 53


>AW688621 weakly similar to SP|P92998|GL11 Germin-like protein subfamily 1
          member 1 precursor. [Mouse-ear cress] {Arabidopsis
          thaliana}, partial (65%)
          Length = 644

 Score = 25.4 bits (54), Expect = 4.6
 Identities = 8/11 (72%), Positives = 10/11 (90%)
 Frame = +2

Query: 22 DPSPLQDFCVA 32
          DP PLQD+C+A
Sbjct: 77 DPDPLQDYCIA 109


>TC86586 weakly similar to GP|15528795|dbj|BAB64690. germin(oxalate
           oxidase)-like protein {Oryza sativa (japonica
           cultivar-group)}, partial (22%)
          Length = 538

 Score = 25.4 bits (54), Expect = 4.6
 Identities = 15/44 (34%), Positives = 21/44 (47%)
 Frame = +2

Query: 2   KILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDGDYSDLT 45
           K+L L++S  A+   I  A DP  L DF        DG++   T
Sbjct: 125 KMLTLIISAFAIVQ-ITLASDPDILTDFIAPTGTQVDGNFFTFT 253


>TC93604 weakly similar to SP|Q9SR72|GL32_ARATH Germin-like protein
           subfamily 3 member 2 precursor. [Mouse-ear cress]
           {Arabidopsis thaliana}, partial (28%)
          Length = 500

 Score = 25.0 bits (53), Expect = 6.0
 Identities = 11/30 (36%), Positives = 18/30 (59%)
 Frame = +3

Query: 3   ILYLLVSILALASSIAFAYDPSPLQDFCVA 32
           +L L+  I+A+     F  DP P+ D+C+A
Sbjct: 96  LLVLMCFIIAV-----FCSDPDPVMDYCIA 170


>AW586190 similar to SP|Q9S8P4|RHRE_ Rhicadhesin receptor precursor
          (Germin-like protein). [Garden pea] {Pisum sativum},
          partial (15%)
          Length = 651

 Score = 24.6 bits (52), Expect = 7.9
 Identities = 13/40 (32%), Positives = 20/40 (49%)
 Frame = +1

Query: 2  KILYLLVSILALASSIAFAYDPSPLQDFCVAIKDPKDGDY 41
          +++ ++  +L L  +     DP  LQD CVA  D   G Y
Sbjct: 58 QMMKIIAVLLLLVLTTTVTADPDNLQDLCVA--DLASGTY 171


>BE319693 
          Length = 605

 Score = 24.6 bits (52), Expect = 7.9
 Identities = 13/28 (46%), Positives = 16/28 (56%)
 Frame = -1

Query: 9   SILALASSIAFAYDPSPLQDFCVAIKDP 36
           S    + SI  +Y  SPLQ FC +I DP
Sbjct: 119 SFFCRSRSICLSY--SPLQGFCGSIVDP 42


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.328    0.145    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,268,450
Number of Sequences: 36976
Number of extensions: 24905
Number of successful extensions: 148
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of query: 59
length of database: 9,014,727
effective HSP length: 35
effective length of query: 24
effective length of database: 7,720,567
effective search space: 185293608
effective search space used: 185293608
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)


Medicago: description of AC140721.8