Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140721.12 - phase: 0 
         (109 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BE941280 weakly similar to GP|20197614|g unknown protein {Arabid...    42  4e-05
CA922316 similar to PIR|F96807|F968 unknown protein T32E8.10 [im...    27  1.2
TC92920 similar to GP|19916011|gb|AAM05485.1 cation efflux syste...    26  2.1
TC81271 weakly similar to GP|20197250|gb|AAC23649.2 Expressed pr...    26  2.1
TC85853 weakly similar to GP|7595348|gb|AAF64423.1| globulin-lik...    25  3.5
TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AA...    25  4.6
TC85852 weakly similar to GP|21593610|gb|AAM65577.1 globulin-lik...    25  4.6
TC82594 similar to GP|5262776|emb|CAB45881.1 berberine bridge en...    25  4.6
TC86562 similar to GP|21553404|gb|AAM62497.1 26S proteasome regu...    25  4.6
TC85936 homologue to GP|13537115|dbj|BAB40755. AtSUG1 {Arabidops...    25  4.6

>BE941280 weakly similar to GP|20197614|g unknown protein {Arabidopsis
           thaliana}, partial (8%)
          Length = 403

 Score = 42.0 bits (97), Expect = 4e-05
 Identities = 20/32 (62%), Positives = 23/32 (71%)
 Frame = -1

Query: 64  LYGYGGTGKTFIWKTSLAVVRSKGLIVLNAAS 95
           LY YGGT KTFIW+   A +RS+G IVL  AS
Sbjct: 403 LYDYGGTEKTFIWRALSAALRSEGEIVLACAS 308


>CA922316 similar to PIR|F96807|F968 unknown protein T32E8.10 [imported] -
           Arabidopsis thaliana, partial (7%)
          Length = 746

 Score = 26.9 bits (58), Expect = 1.2
 Identities = 12/20 (60%), Positives = 12/20 (60%)
 Frame = -1

Query: 52  DSKGFDDGGFFFLYGYGGTG 71
           D  G DDG FF   G GGTG
Sbjct: 662 DELGEDDGDFFGSIGLGGTG 603


>TC92920 similar to GP|19916011|gb|AAM05485.1 cation efflux system protein
           {Methanosarcina acetivorans str. C2A} [Methanosarcina
           acetivorans C2A], partial (3%)
          Length = 658

 Score = 26.2 bits (56), Expect = 2.1
 Identities = 12/36 (33%), Positives = 18/36 (49%)
 Frame = -2

Query: 72  KTFIWKTSLAVVRSKGLIVLNAASRVYREFYWQQTL 107
           KT  WK  L+++    L  LN   +++RE     TL
Sbjct: 318 KTGYWKKCLSLIILLALFCLNTLQKIFRESQHNSTL 211


>TC81271 weakly similar to GP|20197250|gb|AAC23649.2 Expressed protein
           {Arabidopsis thaliana}, partial (30%)
          Length = 870

 Score = 26.2 bits (56), Expect = 2.1
 Identities = 10/15 (66%), Positives = 11/15 (72%)
 Frame = -2

Query: 66  GYGGTGKTFIWKTSL 80
           G GG G+T IWKT L
Sbjct: 188 GGGGAGRTVIWKTEL 144


>TC85853 weakly similar to GP|7595348|gb|AAF64423.1| globulin-like protein
           {Cucumis melo}, partial (39%)
          Length = 666

 Score = 25.4 bits (54), Expect = 3.5
 Identities = 10/20 (50%), Positives = 16/20 (80%)
 Frame = +3

Query: 11  TFTNKLIVDELNYNKDELTK 30
           +F+++LI    N+NKDE+TK
Sbjct: 510 SFSSELISKVYNFNKDEVTK 569


>TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AAA-ATPase
           subunit RPT3 {Arabidopsis thaliana}, partial (94%)
          Length = 1575

 Score = 25.0 bits (53), Expect = 4.6
 Identities = 16/46 (34%), Positives = 21/46 (44%), Gaps = 5/46 (10%)
 Frame = +2

Query: 47  HDKIMDSKGFDDGGFFFLYGYGGTGKTFIWK-----TSLAVVRSKG 87
           H ++    G D      LYG  GTGKT + K     T+ A +R  G
Sbjct: 641 HHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANHTTAAFIRVVG 778


>TC85852 weakly similar to GP|21593610|gb|AAM65577.1 globulin-like protein
           {Arabidopsis thaliana}, partial (17%)
          Length = 1377

 Score = 25.0 bits (53), Expect = 4.6
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +2

Query: 9   MPTFTNKLIVDELNYNKDELTK 30
           + +F++ LI    N+NKDE+TK
Sbjct: 488 LSSFSSDLISKVYNFNKDEVTK 553


>TC82594 similar to GP|5262776|emb|CAB45881.1 berberine bridge enzyme-like
           protein {Arabidopsis thaliana}, partial (26%)
          Length = 698

 Score = 25.0 bits (53), Expect = 4.6
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = -1

Query: 18  VDELNYNKDELTKTQTDMLLMLTDEQRCVHDKI 50
           VDE+N++K  LTK +   ++M T     +H  +
Sbjct: 434 VDEINHDKGCLTKIR*SFIIMATTNNPNLHTNL 336


>TC86562 similar to GP|21553404|gb|AAM62497.1 26S proteasome regulatory
           particle chain RPT6-like protein {Arabidopsis thaliana},
           partial (77%)
          Length = 1564

 Score = 25.0 bits (53), Expect = 4.6
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +1

Query: 64  LYGYGGTGKTFIWKTSLAVVRSKGLIVLN 92
           LYG  GTGKT + K   A+ +  G + +N
Sbjct: 526 LYGPPGTGKTMLAK---AIAKESGAVFIN 603


>TC85936 homologue to GP|13537115|dbj|BAB40755. AtSUG1 {Arabidopsis
           thaliana}, partial (98%)
          Length = 1633

 Score = 25.0 bits (53), Expect = 4.6
 Identities = 18/65 (27%), Positives = 28/65 (42%), Gaps = 9/65 (13%)
 Frame = +1

Query: 47  HDKIMDSKGFDDGGFFFLYGYGGTGKTFIWK-----TSLAVVRSKGLIV----LNAASRV 97
           H ++ +S G        LYG  GTGKT + +     T    +R  G  +    +   SR+
Sbjct: 688 HPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSRM 867

Query: 98  YREFY 102
            RE +
Sbjct: 868 VRELF 882


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.322    0.140    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,151,221
Number of Sequences: 36976
Number of extensions: 33734
Number of successful extensions: 183
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of query: 109
length of database: 9,014,727
effective HSP length: 85
effective length of query: 24
effective length of database: 5,871,767
effective search space: 140922408
effective search space used: 140922408
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)


Medicago: description of AC140721.12