BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140671.6 + phase: 0 /pseudo
(999 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 137 2e-32
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 101 6e-32
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 78 2e-30
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 104 1e-22
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 72 1e-12
BG585499 71 2e-12
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 69 7e-12
TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR re... 64 4e-10
TC90516 53 5e-07
TC82093 35 0.19
AJ497785 similar to GP|21700895|gb| At1g60730/F8A5_24 {Arabidops... 31 2.1
TC85426 similar to SP|O23758|NLTP_CICAR Nonspecific lipid-transf... 31 2.1
TC78185 similar to GP|9955528|emb|CAC05467.1 putative protein {A... 31 2.7
TC81590 similar to GP|6041790|gb|AAF02110.1| putative NADPH-ferr... 30 4.6
TC91625 similar to PIR|D96739|D96739 hypothetical protein F14O23... 30 4.6
AJ502494 similar to GP|7547109|gb| unknown protein {Arabidopsis ... 29 7.9
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 137 bits (346), Expect = 2e-32
Identities = 83/229 (36%), Positives = 120/229 (52%), Gaps = 5/229 (2%)
Frame = +2
Query: 68 WIADGDRNTKYYHSKTIIRRRKNKILTLCDDSSDWIDDPNILKNLVRNFYTDLFKEDTPS 127
W+ DGD+NTK++HSK RR+ N+I L D++ +W ++ L+ ++ +LF P+
Sbjct: 5 WLKDGDKNTKFFHSKASQRRKVNEIKKLKDETGNWCKGEENVERLLITYFNNLFTSSNPT 184
Query: 128 RDSIVSWTTYPNVVGKYHNKLI----SNVQLNECKRALFDMGPHKASGEDGYPAIFFQHC 183
T V GK ++ I E A+ M P KA G DG PA+FFQ
Sbjct: 185 AIE----ETCEVVKGKLSHEHIVWCEKEFTEEEVLEAINQMHPVKAPGPDGLPALFFQKY 352
Query: 184 WDKVADSLFNFVKQVWVNHSLISFINNILIVMIPKIEKPEFVSQFRPISLCNVIYKIISK 243
W V + V QV N +N IV+IPK + P +RPISLCNV+ KII+K
Sbjct: 353 WHIVGKEVQQMVLQVLNNSMETEELNKTFIVLIPKGKNPNTPKDYRPISLCNVVMKIITK 532
Query: 244 VIVNRIKPLLDRIISPYQSSFIPGRNIHHNIIVAQEM-VHAMSKMKGKK 291
VI NR+K L +I QS+F+ GR I N ++A + + + KGK+
Sbjct: 533 VIANRVKQTLPDVIDVEQSAFVQGRLITDNALIAWSVSIG*RXRRKGKR 679
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 101 bits (252), Expect(2) = 6e-32
Identities = 56/172 (32%), Positives = 90/172 (51%), Gaps = 2/172 (1%)
Frame = +3
Query: 28 NSHNYGYSNFLENLEKELHEQLAITLYQEECLWFQKSRSKWIADGDRNTKYYHSKTIIRR 87
N+ + L L EL+E+ + EE W QKSR W+ GDRNTK++H+ T RR
Sbjct: 51 NTKLFNKGEELSLLRSELNEEY----HNEEIFWMQKSRLNWLRSGDRNTKFFHAVTKNRR 218
Query: 88 RKNKILTLC-DDSSDWIDDPNILKNLVRNFYTDLFKEDTPSRDSIVSWTTYPNVVGKYHN 146
+N+IL+L DD +W + ++ + +F ED ++ W + P +V + N
Sbjct: 219 AQNRILSLIDDDDKEWFVEEDLGRLADSHFKLLYSSEDVGI--TLEDWNSIPAIVTEEQN 392
Query: 147 -KLISNVQLNECKRALFDMGPHKASGEDGYPAIFFQHCWDKVADSLFNFVKQ 197
+L++ + E + A+FD+ PHK G DG FFQ WD + D L + ++
Sbjct: 393 AQLMAQISREEVREAVFDINPHKCPGPDGMNVFFFQQFWDTMGDDLTSMAQE 548
Score = 55.5 bits (132), Expect(2) = 6e-32
Identities = 28/63 (44%), Positives = 44/63 (69%)
Frame = +1
Query: 215 MIPKIEKPEFVSQFRPISLCNVIYKIISKVIVNRIKPLLDRIISPYQSSFIPGRNIHHNI 274
++PK + + + +FRPISLCNV YKI+SKV+ R+K +L II+ Q++F + I NI
Sbjct: 601 LVPKKLEAKRLVEFRPISLCNVAYKIVSKVLSKRLKSVLPWIITETQAAFGRRQLISDNI 780
Query: 275 IVA 277
++A
Sbjct: 781 LIA 789
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 78.2 bits (191), Expect(2) = 2e-30
Identities = 45/137 (32%), Positives = 71/137 (50%), Gaps = 1/137 (0%)
Frame = +2
Query: 45 LHEQLAITLYQEECLWFQKSRSKWIADGDRNTKYYHSKTIIRRRKNKILTLCDDSSDWID 104
L E+L EE W QKSR+ W GD N K+YH+ T R +N+I+ L D +WI
Sbjct: 8 LKEKLQEAYKDEEDYWQQKSRNMWHISGDLNKKFYHALTKQRHARNRIVGLYDYDGNWIT 187
Query: 105 DPNILKNLVRNFYTDLFKEDTPSR-DSIVSWTTYPNVVGKYHNKLISNVQLNECKRALFD 163
+ ++ + +++ DLF+ TP+ D + T ++ + + +L+ E + ALF
Sbjct: 188 EEQGVEKVAVDYFEDLFQRTTPTGFDGFLDEIT-SSITPQMNQRLLRLATEEEVRLALFI 364
Query: 164 MGPHKASGEDGYPAIFF 180
M P KA G DG + F
Sbjct: 365 MHPEKAPGPDGMTTLLF 415
Score = 73.6 bits (179), Expect(2) = 2e-30
Identities = 38/89 (42%), Positives = 53/89 (58%)
Frame = +3
Query: 179 FFQHCWDKVADSLFNFVKQVWVNHSLISFINNILIVMIPKIEKPEFVSQFRPISLCNVIY 238
FFQH W + L V + L + +N I +IPK ++P +++ RPISLCNV Y
Sbjct: 411 FFQHSWHIIKMDLLKMVNSFLASGKLDTRLNTTNICLIPKKKRPTRMTELRPISLCNVGY 590
Query: 239 KIISKVIVNRIKPLLDRIISPYQSSFIPG 267
KIISKV+ R+K L +IS QS+F+ G
Sbjct: 591 KIISKVLCQRLKVCLPSLISETQSAFVHG 677
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 104 bits (260), Expect = 1e-22
Identities = 59/156 (37%), Positives = 90/156 (56%)
Frame = +3
Query: 148 LISNVQLNECKRALFDMGPHKASGEDGYPAIFFQHCWDKVADSLFNFVKQVWVNHSLISF 207
L S E K ALF M KA G DGY FF+ W+ + DS+ + + + +
Sbjct: 12 LCSEFTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVIDAILDFFKTGFMPKI 191
Query: 208 INNILIVMIPKIEKPEFVSQFRPISLCNVIYKIISKVIVNRIKPLLDRIISPYQSSFIPG 267
IN + ++PK V FRPI+ C+VIYKIISK++ +R++ +L+ ++S QS+F+ G
Sbjct: 192 INCTYVTLLPKEVNVTSVKNFRPIACCSVIYKIISKILTSRMQGVLNSVVSENQSAFVKG 371
Query: 268 RNIHHNIIVAQEMVHAMSKMKGKKDFMSIKIDL*KS 303
R I NII++ E+V + S+ KG +KIDL K+
Sbjct: 372 RVIFDNIILSHELVKSYSR-KGISPRCMVKIDLXKA 476
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 71.6 bits (174), Expect = 1e-12
Identities = 34/80 (42%), Positives = 56/80 (69%), Gaps = 1/80 (1%)
Frame = -3
Query: 225 VSQFRPISLCNVIYKIISKVIVNRIKPLLDRIISPYQSSFIPGRNIHHNIIVAQEMVHAM 284
VS++R I+ CN YKII+K++ R++PLL IISP QS+F+PGR I N+++ +++H +
Sbjct: 778 VSEYRTIAPCNTQYKIIAKILSKRMQPLLRSIISPSQSAFVPGRAISDNVLITHKILHYL 599
Query: 285 SKMKGKKDF-MSIKIDL*KS 303
+ KK M++K D+ K+
Sbjct: 598 RQSGAKKHVSMAVKTDMTKA 539
>BG585499
Length = 792
Score = 71.2 bits (173), Expect = 2e-12
Identities = 48/93 (51%), Positives = 54/93 (57%), Gaps = 1/93 (1%)
Frame = +1
Query: 773 VVEMKMKLFFMCFAIASTLPRYGFTSFHMIL*LISF-LSIAGIGSSITSSRKTLGRTL*V 831
VVEM KLFFMCF IA RYG +I *LISF L IAGIG S T ++ +
Sbjct: 355 VVEMLTKLFFMCFVIAGQRLRYGLDLCLLIG*LISFPLMIAGIGFSKTLAKDRMEFLSLS 534
Query: 832 GRQLS*QHVGTCGSGGIKLFLKLILLGRTTQFL 864
G QLS* HVG CG G KLFLK + TQ +
Sbjct: 535 GSQLS*LHVGICGLGETKLFLKKDFKDQITQLM 633
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 69.3 bits (168), Expect = 7e-12
Identities = 31/79 (39%), Positives = 50/79 (63%)
Frame = +2
Query: 192 FNFVKQVWVNHSLISFINNILIVMIPKIEKPEFVSQFRPISLCNVIYKIISKVIVNRIKP 251
F FV + N L IN+ I +IPK++ P+ ++ FRPISL +YKI+ K++ NR++
Sbjct: 2 FRFVSEFHRNRKLFKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILGKLLANRLRV 181
Query: 252 LLDRIISPYQSSFIPGRNI 270
++ +IS QS+F+ R I
Sbjct: 182 VIGSVISDAQSAFVKNRQI 238
>TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 798
Score = 63.5 bits (153), Expect = 4e-10
Identities = 34/93 (36%), Positives = 48/93 (51%), Gaps = 1/93 (1%)
Frame = -1
Query: 1 LKEWNTKTFGNIFRRKNELLARLNGIQ-NSHNYGYSNFLENLEKELHEQLAITLYQEECL 59
LK WN TFGN+ + + L+ IQ + GYS+ L + EK L L EE
Sbjct: 282 LKVWNKNTFGNVHSQVDNAYKELDDIQVKIDSIGYSDVLMDQEKAAQLNLESALNIEEVF 103
Query: 60 WFQKSRSKWIADGDRNTKYYHSKTIIRRRKNKI 92
W +KS+ W +GDRNT ++H I+R + I
Sbjct: 102 WHEKSKVNWHCEGDRNTAFFHRVAKIKRTSSLI 4
>TC90516
Length = 983
Score = 53.1 bits (126), Expect = 5e-07
Identities = 47/237 (19%), Positives = 104/237 (43%), Gaps = 10/237 (4%)
Frame = -2
Query: 58 CLWFQKSRSKWIADGDR---------NTKYYHSKTIIRRRKNKILTLCDDSSDWIDDPNI 108
C F +S+++ ++G NT Y+ + R +N I W+DD
Sbjct: 820 CKLFCRSKTRRCSNGQEQSG*EREGHNTTYFLACVKNRGMRNSISA--PRVERWMDDVAK 647
Query: 109 LKNLVRNFYTDLFKEDTPSRDSIVSWTTYPNVVGKYHNKLIS-NVQLNECKRALFDMGPH 167
K + NF+T F + R ++ + + N L+S +++ + + + +
Sbjct: 646 NKQAIVNFFTHHFSDP*TYRPTMGDIDFFH--ISNLDNVLLSAQFLVSKMELVVSSLDGN 473
Query: 168 KASGEDGYPAIFFQHCWDKVADSLFNFVKQVWVNHSLISFINNILIVMIPKIEKPEFVSQ 227
++ DG+ FF + + + +Q + +L+ + + +I K+E P +
Sbjct: 472 ESPRPDGFNLNFFIRLRNMLKADIEIMFEQFYTPANLLKIFS*YFLTLISKVEYPILLGD 293
Query: 228 FRPISLCNVIYKIISKVIVNRIKPLLDRIISPYQSSFIPGRNIHHNIIVAQEMVHAM 284
F +S + K+++KV+ R+ ++++II QS+F+ GR ++ E++ M
Sbjct: 292 FSLMSFLGSL*KLMAKVLALRLAHIMEKIIFVNQSTFVRGRQHVDGVVAINEIIVLM 122
>TC82093
Length = 569
Score = 34.7 bits (78), Expect = 0.19
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = -1
Query: 53 LYQEECLWFQKSRSKWIADGDRNTKYYHSKTI 84
L Q E LW+ KS+ W+ G RNT ++H++ +
Sbjct: 473 LSQNEMLWY*KSQ*NWVKYGSRNTWFFHNEDV 378
>AJ497785 similar to GP|21700895|gb| At1g60730/F8A5_24 {Arabidopsis
thaliana}, partial (7%)
Length = 460
Score = 31.2 bits (69), Expect = 2.1
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = -2
Query: 6 TKTFGNIFRRKNELLARLNGIQNSHN-YGYSNFLENLEKELHEQLA 50
TK NIF+ K +L +L G N H Y + L+NLE+ L L+
Sbjct: 204 TKEVHNIFKNKKKLKIKLGGKHNMHGFYSTLSALKNLEEPLVSNLS 67
>TC85426 similar to SP|O23758|NLTP_CICAR Nonspecific lipid-transfer protein
precursor (LTP). [Chickpea Garbanzo] {Cicer arietinum},
complete
Length = 696
Score = 31.2 bits (69), Expect = 2.1
Identities = 20/46 (43%), Positives = 26/46 (56%)
Frame = +2
Query: 914 WGLLLDVAAFFVIQTVDGSKATLRRLELVMPYMLRCGDYIWVWTWL 959
W L+L VA F+ QTV S L L ++ Y L G+ WV+TWL
Sbjct: 512 WRLILYVALSFLYQTVFDS---LLPLHVLFLYEL-LGEIFWVFTWL 637
>TC78185 similar to GP|9955528|emb|CAC05467.1 putative protein {Arabidopsis
thaliana}, partial (24%)
Length = 1351
Score = 30.8 bits (68), Expect = 2.7
Identities = 17/59 (28%), Positives = 29/59 (48%), Gaps = 9/59 (15%)
Frame = -2
Query: 207 FINNILIVMIPK---------IEKPEFVSQFRPISLCNVIYKIISKVIVNRIKPLLDRI 256
F NN +V+IP+ + K + +P + N+I +K+ N+IKP L R+
Sbjct: 1350 FFNN*HLVIIPRPQSTFVQILVSKXSYTIMLQP*PIINIILSSHNKITTNKIKPCLIRL 1174
>TC81590 similar to GP|6041790|gb|AAF02110.1| putative
NADPH-ferrihemoprotein reductase {Arabidopsis thaliana},
partial (33%)
Length = 1095
Score = 30.0 bits (66), Expect = 4.6
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +3
Query: 176 PAIFFQHCWDKVADSLFNFVKQVWVNHS 203
P +FF CW++ D L+ K +W+NHS
Sbjct: 330 PIMFFFGCWNEDGDFLY---KDLWLNHS 404
>TC91625 similar to PIR|D96739|D96739 hypothetical protein F14O23.9
[imported] - Arabidopsis thaliana, partial (12%)
Length = 621
Score = 30.0 bits (66), Expect = 4.6
Identities = 18/81 (22%), Positives = 38/81 (46%), Gaps = 5/81 (6%)
Frame = -1
Query: 199 WVNHSLISFINNILIVMIPKIEKPEFVSQFRPISLCNVIYKIISKVIV-----NRIKPLL 253
+++H + F+ + + IP + P+ LC IYK+ + V + + I+ L
Sbjct: 471 YLDHQVEVFLQHSMFRHIPSV----------PLCLCTEIYKLTTSVSLTLEQSHLIQTEL 322
Query: 254 DRIISPYQSSFIPGRNIHHNI 274
+ P +F P ++HH++
Sbjct: 321 QNLCCP*TLTFFPTLHVHHHL 259
>AJ502494 similar to GP|7547109|gb| unknown protein {Arabidopsis thaliana},
partial (48%)
Length = 696
Score = 29.3 bits (64), Expect = 7.9
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = -2
Query: 207 FINNILIVMIPKIEKPEFVSQFRPISLCNVIYKIISKVIVNR 248
F NI+I IPKI E + FRP+S ++ + S NR
Sbjct: 581 FRKNIIIDFIPKITAEEPIIIFRPLSKNRILPSLTSSFTRNR 456
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.360 0.161 0.604
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,577,749
Number of Sequences: 36976
Number of extensions: 562234
Number of successful extensions: 5609
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1697
Number of HSP's successfully gapped in prelim test: 247
Number of HSP's that attempted gapping in prelim test: 3803
Number of HSP's gapped (non-prelim): 2156
length of query: 999
length of database: 9,014,727
effective HSP length: 106
effective length of query: 893
effective length of database: 5,095,271
effective search space: 4550077003
effective search space used: 4550077003
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.9 bits)
S2: 63 (28.9 bits)
Medicago: description of AC140671.6