BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140549.12 - phase: 0
(504 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG645818 PIR|S58801|S58 ubiquinol--cytochrome-c reductase (EC 1.... 36 0.030
TC77672 33 0.26
BQ124307 33 0.26
TC78475 similar to GP|20466227|gb|AAM20431.1 acyl-CoA oxidase AC... 33 0.26
TC85484 similar to PIR|S58083|S58083 transketolase (EC 2.2.1.1) ... 32 0.44
CB893096 32 0.44
TC79561 similar to GP|14194107|gb|AAK56248.1 At1g77260/T14N5_19 ... 31 1.3
TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 ... 31 1.3
TC89433 similar to GP|13277220|emb|CAC34409. ZF-HD homeobox prot... 30 1.7
TC79733 similar to PIR|T01797|T01797 hypothetical protein A_TM02... 30 1.7
AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing fact... 30 1.7
BG583244 30 2.2
TC80046 similar to SP|P22699|GUN6_DICDI Endoglucanase precursor ... 30 2.2
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 30 2.8
TC78961 similar to GP|18252179|gb|AAL61922.1 unknown protein {Ar... 30 2.8
TC78771 similar to GP|16612268|gb|AAL27502.1 AT3g61860/F21F14_30... 29 3.7
AL368496 similar to GP|16612268|gb| AT3g61860/F21F14_30 {Arabido... 29 3.7
AW689398 similar to GP|21593065|gb| NAM / CUC2-like protein {Ara... 29 4.8
TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AA... 28 6.3
TC85059 similar to GP|10178116|dbj|BAB11409. ubiquitin carboxyl-... 28 8.3
>BG645818 PIR|S58801|S58 ubiquinol--cytochrome-c reductase (EC 1.10.2.2)
cytochrome b - Leptothorax acervorum mitochondrion,
partial (10%)
Length = 701
Score = 36.2 bits (82), Expect = 0.030
Identities = 23/68 (33%), Positives = 32/68 (46%), Gaps = 4/68 (5%)
Frame = +2
Query: 203 PIKVDTNTLKVERGKFARVCVEIDLTVPVVGKIWVNGHWY----KVQYEGLHLICTNCGC 258
P+ +D TL G FARV V++DL+ + + V Y +QYE NC
Sbjct: 26 PLAIDDATLSRRFGVFARVLVDVDLSEQLFESVVVEIEGYILSIDIQYEK*PPF*VNCKV 205
Query: 259 YGHLGRNC 266
GH +NC
Sbjct: 206 LGHNLQNC 229
>TC77672
Length = 766
Score = 33.1 bits (74), Expect = 0.26
Identities = 35/148 (23%), Positives = 59/148 (39%), Gaps = 6/148 (4%)
Frame = +2
Query: 328 KDVTANKRNAINRIDDNQVLHGDWLVVTRRKKTPIQHSLNASKSVTHKTNRFQ----ALS 383
KD + AI+ + NQV WL T+ +K + TH +N+
Sbjct: 2 KDTPEENKKAIH--NTNQVYLDGWLKDTKNQK----------EKTTHNSNQVYLDGWLKD 145
Query: 384 TMTHHDKSAPINNK--FPSWSKSQEIPRANNRSTETKRRRQEDFYEPIAKNLSTNDSRHI 441
T KS P +N+ W K ++ +A + S + +P+ K ST +S +
Sbjct: 146 TQVQKTKSTPNSNQVYLDGWLKDTQVEKAKSTSDSNQVYLDGWLKDPVEKAKSTPNSNQV 325
Query: 442 TILEPVLSLKTKDTSTSKIDITQPHSTE 469
L KD+ K +I+ P+S +
Sbjct: 326 -----YLDGWLKDSQVEK-EISNPNSNQ 391
>BQ124307
Length = 762
Score = 33.1 bits (74), Expect = 0.26
Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 1/85 (1%)
Frame = +3
Query: 420 RRQEDFYEPIAKNLSTNDSRHIT-ILEPVLSLKTKDTSTSKIDITQPHSTEHVTNPSQNN 478
RR E + K STN + + +++ +S KD S ++ + P NP+
Sbjct: 348 RRSERIRSGVVKVKSTNSKQGVECVVDMTVSDSEKDESDAQTEQVLPQP-----NPTDTQ 512
Query: 479 HEPVTTYTEDDTIPDPSSSMPQDTP 503
EPV +++ +P +PQ+ P
Sbjct: 513 TEPVQVLPQENRQTEPVQVLPQENP 587
>TC78475 similar to GP|20466227|gb|AAM20431.1 acyl-CoA oxidase ACX3
{Arabidopsis thaliana}, partial (90%)
Length = 2177
Score = 33.1 bits (74), Expect = 0.26
Identities = 24/73 (32%), Positives = 34/73 (45%), Gaps = 4/73 (5%)
Frame = +2
Query: 272 TFDPRSPNHHTAGGNNPPSNPEPSQP---RQNPTQTC-PDAINSQLMACNQNGNSIIDHN 327
T D P HH+A N+ P PS+P NP Q C P ++S+ + N I H
Sbjct: 38 TIDHGQPPHHSA--NHHSHEPPPSRPPNRTNNPPQPCLP*PLSSRTLRIKSNN---IRHL 202
Query: 328 KDVTANKRNAINR 340
+D A +R+ R
Sbjct: 203 RDAEALRRSQFGR 241
>TC85484 similar to PIR|S58083|S58083 transketolase (EC 2.2.1.1) precursor -
potato (fragment), partial (95%)
Length = 2685
Score = 32.3 bits (72), Expect = 0.44
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = +1
Query: 435 TNDSRHITILEPVLSLKTKDTSTSKIDITQPHSTEHVTNPSQNNHEPVTTYTEDDTIPDP 494
TN H L LS + +STS + +T PH T H ++PS + + + P P
Sbjct: 49 TNQLSHSHFLSLSLSTMSSSSSTS-LYLTNPHLTRHTSSPSTTRLSSLPSTITLNRTPTP 225
Query: 495 SSSMPQ 500
++ + Q
Sbjct: 226 TTHLSQ 243
>CB893096
Length = 843
Score = 32.3 bits (72), Expect = 0.44
Identities = 53/265 (20%), Positives = 96/265 (36%), Gaps = 23/265 (8%)
Frame = +1
Query: 255 NCGCYGHLGRNCMEKPSTFDPRSPNHHTAG---GNNPPSNPEPSQPRQNP--TQTCPDAI 309
N G +G + ++ P P + G G N PSN S +QNP Q+ PD
Sbjct: 127 NSGNFGDKQQQALKGGVEVIPSQPFAVSFGSFNGTNLPSNLNFSSMKQNPLVIQSLPDVA 306
Query: 310 NSQLMACNQNGNSIIDHNKDVTANKR--NAINRIDDNQVLHGDWLVVTRRKKTPIQHSLN 367
A + + + N T KR N+ ++ D+ + HG + S N
Sbjct: 307 RQGYQAAS-TSHIVQQKNYSTTIEKRGGNSSHKDDEKKTTHG-------------KSSAN 444
Query: 368 ASKSVTHKTNRFQALSTMTHHDKSAPINNKFPSWSKSQEIPRANNRSTETKRRRQEDFYE 427
++ + ++H N +PS S + + S+ T +Q
Sbjct: 445 GPTTLVFDNSSKNINFVLSHS------NGNWPSHSITSTVTTTMPFSSNTSSSQQS---- 594
Query: 428 PIAKNLSTNDSRHITILEPVLSLKTKDTSTSKIDIT----------QPHSTEHVTNPSQN 477
A++L + + +P + + K +ST+ T Q H+ + +N + +
Sbjct: 595 --AQSLQLHKQHGMQQQQPAAATRNKASSTNTTSATNFANNGPVFSQSHTQCNSSNQTSH 768
Query: 478 ------NHEPVTTYTEDDTIPDPSS 496
NH +T TI P++
Sbjct: 769 SKITGRNHSYHVHHTPSTTIKTPTT 843
>TC79561 similar to GP|14194107|gb|AAK56248.1 At1g77260/T14N5_19
{Arabidopsis thaliana}, partial (33%)
Length = 1092
Score = 30.8 bits (68), Expect = 1.3
Identities = 18/61 (29%), Positives = 25/61 (40%), Gaps = 4/61 (6%)
Frame = +2
Query: 273 FDPRSPNHHTAGGNNPPSNPEPSQPRQNPTQTCPDAINSQLMACNQNG----NSIIDHNK 328
F P SP H PP P P QP P + P ++ ++NG N + +N
Sbjct: 122 FSPPSPEDHQQQQPPPPPPPPPLQPTPKPFRIPPLPPQERIGLIDENGVMTDNFTVSNND 301
Query: 329 D 329
D
Sbjct: 302 D 304
>TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 - alfalfa,
partial (97%)
Length = 1049
Score = 30.8 bits (68), Expect = 1.3
Identities = 18/56 (32%), Positives = 25/56 (44%)
Frame = +3
Query: 256 CGCYGHLGRNCMEKPSTFDPRSPNHHTAGGNNPPSNPEPSQPRQNPTQTCPDAINS 311
C + +G + PST SP T N PP+ P+ S P P Q+ P + S
Sbjct: 207 CVVFSSVG--AQQAPSTSPNSSPAPPTPPANTPPTTPQASPP---PVQSSPPPVQS 359
>TC89433 similar to GP|13277220|emb|CAC34409. ZF-HD homeobox protein
{Flaveria bidentis}, partial (52%)
Length = 1430
Score = 30.4 bits (67), Expect = 1.7
Identities = 20/62 (32%), Positives = 27/62 (43%), Gaps = 6/62 (9%)
Frame = +1
Query: 253 CTNCGCYGHLGRNCMEKP-STFDPRSPNHHTAGGNNPPSNP-----EPSQPRQNPTQTCP 306
C CGC+ + R E+P ST P+H ++PP P PS P P + P
Sbjct: 619 CAACGCHRNFHRREPEEPISTVFEYQPHHR----HHPPPPPLFQSRSPSSPSPPPISSYP 786
Query: 307 DA 308
A
Sbjct: 787 SA 792
>TC79733 similar to PIR|T01797|T01797 hypothetical protein A_TM021B04.9 -
Arabidopsis thaliana, partial (2%)
Length = 1253
Score = 30.4 bits (67), Expect = 1.7
Identities = 18/49 (36%), Positives = 28/49 (56%), Gaps = 3/49 (6%)
Frame = +2
Query: 450 LKTKDTSTSKIDITQPHSTE---HVTNPSQNNHEPVTTYTEDDTIPDPS 495
L+ KDTSTSK+++ + +S E HV + + P + E +PDPS
Sbjct: 230 LELKDTSTSKVNLERSNSVEEKDHVDH-IDDVRAPKVSVAESFEVPDPS 373
>AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing factor
[imported] - Arabidopsis thaliana, partial (62%)
Length = 508
Score = 30.4 bits (67), Expect = 1.7
Identities = 18/54 (33%), Positives = 20/54 (36%)
Frame = +2
Query: 248 GLHLICTNCGCYGHLGRNCMEKPSTFDPRSPNHHTAGGNNPPSNPEPSQPRQNP 301
G L C CG GH R C P GG+ NP P Q +NP
Sbjct: 329 GSDLKCYXCGEPGHFARXCNSSP-------------GGSGXRRNPSPPQFXRNP 451
>BG583244
Length = 633
Score = 30.0 bits (66), Expect = 2.2
Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
Frame = -1
Query: 217 KFARVCVEIDLTVPVVGKIWVNGH-----WYKVQYEGLHLICTNCGCYGH 261
K+ ++ VEI++ P+ + + G W +YE L C CG GH
Sbjct: 156 KYVKIRVEIEIEQPLKQDLILEGDMCKNIWLVFKYEKLDKFCFVCGSIGH 7
>TC80046 similar to SP|P22699|GUN6_DICDI Endoglucanase precursor (EC
3.2.1.4) (Endo-1 4-beta-glucanase) (Spore germination
protein 270-6), partial (11%)
Length = 643
Score = 30.0 bits (66), Expect = 2.2
Identities = 22/63 (34%), Positives = 28/63 (43%)
Frame = +3
Query: 441 ITILEPVLSLKTKDTSTSKIDITQPHSTEHVTNPSQNNHEPVTTYTEDDTIPDPSSSMPQ 500
ITI+EPV T TS IT S VT+ S N P T T +T + +
Sbjct: 168 ITIIEPV-------TVTSTATITTTVSPITVTDTSTNTVSPSPTETSTETSTETPTETST 326
Query: 501 DTP 503
+TP
Sbjct: 327 ETP 335
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 29.6 bits (65), Expect = 2.8
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = +3
Query: 263 GRNCMEKPSTFDPRSPNHHTAGGNNPPSNPEPSQPRQ 299
G N ++P DP P H AGG P P P P++
Sbjct: 567 GGNTQKEPRGEDP--PPHTPAGGGGGPGGPRPPPPKK 671
>TC78961 similar to GP|18252179|gb|AAL61922.1 unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 974
Score = 29.6 bits (65), Expect = 2.8
Identities = 17/65 (26%), Positives = 27/65 (41%), Gaps = 3/65 (4%)
Frame = +2
Query: 237 VNGHWYKVQYEG-LHLICTNCGCYGHLGRNCMEKPSTFD--PRSPNHHTAGGNNPPSNPE 293
++GHW + G C CG GH+ +NC P RS + +P +
Sbjct: 374 IDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKKLSRHARSVSRSPGRSRSPARSRS 553
Query: 294 PSQPR 298
P++ R
Sbjct: 554 PARSR 568
>TC78771 similar to GP|16612268|gb|AAL27502.1 AT3g61860/F21F14_30
{Arabidopsis thaliana}, partial (87%)
Length = 1254
Score = 29.3 bits (64), Expect = 3.7
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = +1
Query: 99 SLGYNTMKDRLQKIWKLQGGFDIMDNDNGFFMVKFDQAADKEKVITGGPWLIFDH 153
+L Y+T + L++++ G + +D +GF V ++ D E+ I + F H
Sbjct: 109 NLEYDTRQSELERLFSKYGRIERVDMKSGFAFVYYEDERDAEEAIRALDNIPFGH 273
>AL368496 similar to GP|16612268|gb| AT3g61860/F21F14_30 {Arabidopsis
thaliana}, partial (36%)
Length = 616
Score = 29.3 bits (64), Expect = 3.7
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = -2
Query: 99 SLGYNTMKDRLQKIWKLQGGFDIMDNDNGFFMVKFDQAADKEKVITGGPWLIFDH 153
+L Y+T + L++++ G + +D +GF V ++ D E+ I + F H
Sbjct: 585 NLEYDTRQSELERLFSKYGRIERVDMKSGFAFVYYEDERDAEEAIRALDNIPFGH 421
>AW689398 similar to GP|21593065|gb| NAM / CUC2-like protein {Arabidopsis
thaliana}, partial (13%)
Length = 651
Score = 28.9 bits (63), Expect = 4.8
Identities = 23/92 (25%), Positives = 42/92 (45%), Gaps = 2/92 (2%)
Frame = +2
Query: 411 NNRSTETKRRRQEDFYEPIAKNLSTNDSRHITILEPVLSLKTKDTSTSKID-ITQPHSTE 469
NN + +K Q+ YE +L+ S + +T + +K+D + P +T
Sbjct: 176 NNNNNSSKLNTQQFMYETTPPSLTLMSSSPT-------NYQTIPSGYNKLDSFSSPMTTL 334
Query: 470 HVTNPSQNNHEPVTTYT-EDDTIPDPSSSMPQ 500
H NP+QNN + +T P+ +S++ Q
Sbjct: 335 HHLNPNQNNSSMMNLLQYSRETNPNDNSTVTQ 430
>TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AAA-ATPase
subunit RPT3 {Arabidopsis thaliana}, partial (94%)
Length = 1575
Score = 28.5 bits (62), Expect = 6.3
Identities = 21/59 (35%), Positives = 23/59 (38%)
Frame = +3
Query: 277 SPNHHTAGGNNPPSNPEPSQPRQNPTQTCPDAINSQLMACNQNGNSIIDHNKDVTANKR 335
SPNHH PP P P P PT + P + S N N NS NKR
Sbjct: 129 SPNHHPLFHPPPPPTPIPPPP---PTTSTPSSNPS-----NANSNSSTYKKSMSKMNKR 281
>TC85059 similar to GP|10178116|dbj|BAB11409. ubiquitin carboxyl-terminal
hydrolase {Arabidopsis thaliana}, partial (10%)
Length = 561
Score = 28.1 bits (61), Expect = 8.3
Identities = 17/72 (23%), Positives = 28/72 (38%), Gaps = 6/72 (8%)
Frame = +2
Query: 432 NLSTNDSRHITILEPVLSLKTKDTSTSKIDITQPHSTEHVTNPSQNNHEPVTTYTEDDT- 490
N T S IT++ + ++ PH+ H NNH+P+ + +T
Sbjct: 38 NNRTARSISITVMTIMTPAPIDQQQQEDEEMLVPHAVPHADLAENNNHQPMDVVAQPETA 217
Query: 491 -----IPDPSSS 497
+ DPS S
Sbjct: 218 NTVEPVEDPSPS 253
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,254,397
Number of Sequences: 36976
Number of extensions: 274959
Number of successful extensions: 1906
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 1841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1900
length of query: 504
length of database: 9,014,727
effective HSP length: 100
effective length of query: 404
effective length of database: 5,317,127
effective search space: 2148119308
effective search space used: 2148119308
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC140549.12