BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140546.3 + phase: 0
(266 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87707 similar to PIR|B84632|B84632 probable chloroplast initia... 517 e-147
TC79661 weakly similar to PIR|H86467|H86467 probable transcripti... 59 2e-09
TC86724 homologue to PIR|T06445|T06445 GTP-binding protein - gar... 35 0.039
AL375304 homologue to PIR|T06445|T06 GTP-binding protein - garde... 34 0.051
TC81774 31 0.57
TC84723 similar to GP|16945387|emb|CAD11800. conserved hypotheti... 30 0.96
AW685168 weakly similar to PIR|T01757|T0 hypothetical protein A_... 30 1.3
TC87569 similar to PIR|E96776|E96776 hypothetical protein F25A4.... 28 4.8
BG646741 weakly similar to GP|17978960|g AT5g10020/T31P16_9 {Ara... 27 6.3
TC78952 weakly similar to GP|21554042|gb|AAM63123.1 AX110P-like ... 27 6.3
TC77199 similar to SP|O04379|AGO1_ARATH Argonaute protein. [Mous... 27 6.3
TC91375 similar to GP|19570997|dbj|BAB86424. contains ESTs AU165... 27 8.2
TC81198 similar to GP|21553392|gb|AAM62485.1 unknown {Arabidopsi... 27 8.2
>TC87707 similar to PIR|B84632|B84632 probable chloroplast initiation factor
3 [imported] - Arabidopsis thaliana, partial (55%)
Length = 1249
Score = 517 bits (1332), Expect = e-147
Identities = 266/266 (100%), Positives = 266/266 (100%)
Frame = +1
Query: 1 MAGITSTTVVPVKLKASHFFSNKLSIPHSLNLQSSPSFTLPSHATVRYGVNLRPSAYGGG 60
MAGITSTTVVPVKLKASHFFSNKLSIPHSLNLQSSPSFTLPSHATVRYGVNLRPSAYGGG
Sbjct: 37 MAGITSTTVVPVKLKASHFFSNKLSIPHSLNLQSSPSFTLPSHATVRYGVNLRPSAYGGG 216
Query: 61 GNFRRAPPEKDADDGQALDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIV 120
GNFRRAPPEKDADDGQALDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIV
Sbjct: 217 GNFRRAPPEKDADDGQALDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIV 396
Query: 121 SAEADPPVVRIMNYSKYRYELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAA 180
SAEADPPVVRIMNYSKYRYELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAA
Sbjct: 397 SAEADPPVVRIMNYSKYRYELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAA 576
Query: 181 RKFLSDGDKVKVIVNLKGREKEFRNNAIELIRRFQNDVGKLATEEAKNFRDKNIFITLIP 240
RKFLSDGDKVKVIVNLKGREKEFRNNAIELIRRFQNDVGKLATEEAKNFRDKNIFITLIP
Sbjct: 577 RKFLSDGDKVKVIVNLKGREKEFRNNAIELIRRFQNDVGKLATEEAKNFRDKNIFITLIP 756
Query: 241 NKTEVQKPQETPTKAATDEVSVSVEA 266
NKTEVQKPQETPTKAATDEVSVSVEA
Sbjct: 757 NKTEVQKPQETPTKAATDEVSVSVEA 834
>TC79661 weakly similar to PIR|H86467|H86467 probable transcription factor
66207-62663 [imported] - Arabidopsis thaliana, partial
(13%)
Length = 1938
Score = 58.9 bits (141), Expect = 2e-09
Identities = 37/126 (29%), Positives = 65/126 (51%), Gaps = 2/126 (1%)
Frame = +2
Query: 69 EKDADDGQALDLSTLSSNTVRLIDQSQNMVGVVSLDQAIRMAEDAELDLVIVSAEADPPV 128
E+D DG L+ + + VRL+ ++ +VS +A+ A +LDLV V + PPV
Sbjct: 260 EEDDTDGPRLN-DQIKARQVRLVVDGEH--SIVSRFEALERARKLKLDLVEVDKNSTPPV 430
Query: 129 VRIMNYSKYRYELQK--KKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAARKFLSD 186
+IM+Y K Y+ ++ K+R + K + + KE++ + D ++ RK +
Sbjct: 431 CKIMDYHKEMYKKKENFKERAKSKSEMTMKKECKEVRFSEKTESKDLKMKSDMVRKLMEK 610
Query: 187 GDKVKV 192
G +VKV
Sbjct: 611 GYRVKV 628
>TC86724 homologue to PIR|T06445|T06445 GTP-binding protein - garden pea,
complete
Length = 1037
Score = 34.7 bits (78), Expect = 0.039
Identities = 14/35 (40%), Positives = 24/35 (68%)
Frame = +3
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
EE +N DKNI I L+ NK++++ ++ PT+ A +
Sbjct: 510 EELRNHADKNIVIILVGNKSDLENQRDVPTEDAKE 614
>AL375304 homologue to PIR|T06445|T06 GTP-binding protein - garden pea,
partial (64%)
Length = 497
Score = 34.3 bits (77), Expect = 0.051
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = +2
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
EE +N DKNI I LI NK+++++ + PT+ A +
Sbjct: 389 EELRNHADKNIVIILIGNKSDLEEQRAVPTEDAKE 493
>TC81774
Length = 801
Score = 30.8 bits (68), Expect = 0.57
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = -1
Query: 217 DVGKLATEEAKNFRDKNIFITLIPNKTEVQK 247
D+G L + AK+F+D FIT++ K + K
Sbjct: 387 DMGNLTQKTAKSFKDSTFFITILHTKNNILK 295
>TC84723 similar to GP|16945387|emb|CAD11800. conserved hypothetical protein
{Neurospora crassa}, partial (5%)
Length = 999
Score = 30.0 bits (66), Expect = 0.96
Identities = 40/160 (25%), Positives = 68/160 (42%), Gaps = 10/160 (6%)
Frame = +3
Query: 114 ELDLVIVSAEADPPVVRIMNYSKYRYELQKKKR--DQQKKSAASRMDLKELKMGYNIDQH 171
EL + +S + +R+ + ELQ+ KR D+ + A + K LK + ++
Sbjct: 519 ELRELKISKAREREELRVQKEYREEAELQRAKRDLDEIRNREARISEEKHLKSQVELQKY 698
Query: 172 DYSVRL--KAARKFLSDGDKV-----KVIVNLKGREKEFRNNAIELIRRFQNDVGKLATE 224
+RL KA ++ D V K L +EKE + E RR Q + K
Sbjct: 699 QEEMRLAEKAKQREKEAADAVERFQKKEQERLAKQEKEKQEREKEFQRRLQEERQKELDR 878
Query: 225 EAKNFRDKNIFITLIPNKTEVQKP-QETPTKAATDEVSVS 263
AK ++K N+ E Q+ +E K+ DE +++
Sbjct: 879 IAKAKKEKE------ENEKEYQRRLREDLIKSGLDEKAIA 980
>AW685168 weakly similar to PIR|T01757|T0 hypothetical protein A_IG002P16.20
- Arabidopsis thaliana, partial (17%)
Length = 607
Score = 29.6 bits (65), Expect = 1.3
Identities = 25/94 (26%), Positives = 44/94 (46%), Gaps = 2/94 (2%)
Frame = +2
Query: 95 QNMVGVVSLDQAIRMAEDAELDLVIVSAEADPPVVRIMNYSKYRYELQKKKRDQQKKSAA 154
Q V S ++ I+ +D LVI A AD + +ELQK+ +K S+
Sbjct: 221 QLQVSTSSAEKHIKELDDLRTQLVITRATADSSAASTQSSQLQCFELQKEL--DEKNSSL 394
Query: 155 SRMDLKELKMGYNID--QHDYSVRLKAARKFLSD 186
+ + +++G +D Q D R ++++K L D
Sbjct: 395 REHEERVIRLGEQLDNLQKDLKSR-ESSQKQLRD 493
>TC87569 similar to PIR|E96776|E96776 hypothetical protein F25A4.30
[imported] - Arabidopsis thaliana, partial (12%)
Length = 885
Score = 27.7 bits (60), Expect = 4.8
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -3
Query: 20 FSNKLSIPHSLNLQSSPSFTLPSHAT 45
FSNKLS P S L+ PSF++ T
Sbjct: 538 FSNKLSKPSSSALEKRPSFSIEESVT 461
>BG646741 weakly similar to GP|17978960|g AT5g10020/T31P16_9 {Arabidopsis
thaliana}, partial (9%)
Length = 475
Score = 27.3 bits (59), Expect = 6.3
Identities = 17/44 (38%), Positives = 26/44 (58%), Gaps = 6/44 (13%)
Frame = -1
Query: 176 RLKAARKFLSDGDKVK------VIVNLKGREKEFRNNAIELIRR 213
RL+A+ K S G++ K + + LK R +FRNNAIE ++
Sbjct: 217 RLRASLKTRSPGNEPKEGSSPLIKLPLKSRVCKFRNNAIESFKK 86
>TC78952 weakly similar to GP|21554042|gb|AAM63123.1 AX110P-like protein
{Arabidopsis thaliana}, partial (58%)
Length = 858
Score = 27.3 bits (59), Expect = 6.3
Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -3
Query: 1 MAGITSTTVVPVKLKASHFFSNKLSIPH-SLNLQSSPSFT 39
M + T + ++ K HFFS+++ PH S+++ S FT
Sbjct: 559 MYRLKGTKEIRIRYKLKHFFSSRMMHPHSSIHILQSIRFT 440
>TC77199 similar to SP|O04379|AGO1_ARATH Argonaute protein. [Mouse-ear cress]
{Arabidopsis thaliana}, partial (74%)
Length = 2714
Score = 27.3 bits (59), Expect = 6.3
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Frame = -2
Query: 1 MAGITSTTVVP--VKLKASHFFSNKLSIPHSLNLQSSPSFTLPSHATVRY 48
+ G T +P +KLK H KLS+ HS++++ L S +T RY
Sbjct: 1840 IVGFKGCTSLPDRIKLKQQHLV--KLSLAHSISVKDDALGLLSSSSTERY 1697
>TC91375 similar to GP|19570997|dbj|BAB86424. contains ESTs AU165675(E3969)
C73379(E3969)~similar to Arabidopsis thaliana
chromosome5 MMI9.10~, partial (67%)
Length = 1062
Score = 26.9 bits (58), Expect = 8.2
Identities = 12/38 (31%), Positives = 19/38 (49%)
Frame = +1
Query: 139 YELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVR 176
YE+ KK+ D + +M+ + KM + H YS R
Sbjct: 784 YEISKKEDDMLYRDFVEKMNFNKKKMAGEVKCHKYSRR 897
>TC81198 similar to GP|21553392|gb|AAM62485.1 unknown {Arabidopsis
thaliana}, partial (38%)
Length = 913
Score = 26.9 bits (58), Expect = 8.2
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +1
Query: 22 NKLSIPHSLNLQSSPSFTLPSHATVRYGVNLRPSAY 57
+KL + H Q+SPS T PSH + + + + S++
Sbjct: 52 HKLILCHLRESQTSPSLTYPSHPSSLFPLPINTSSF 159
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.130 0.350
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,731,954
Number of Sequences: 36976
Number of extensions: 62326
Number of successful extensions: 384
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 384
length of query: 266
length of database: 9,014,727
effective HSP length: 94
effective length of query: 172
effective length of database: 5,538,983
effective search space: 952705076
effective search space used: 952705076
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC140546.3