BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139841.9 + phase: 0
(500 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87858 similar to GP|9757903|dbj|BAB08350.1 gene_id:MMN10.5~unk... 487 0.0
AW774768 similar to GP|3236235|gb|A unknown protein {Arabidopsis... 140 9e-34
TC88101 similar to PIR|B86182|B86182 hypothetical protein [impor... 70 2e-12
BF645514 62 4e-10
BE239959 similar to GP|9758032|dbj| gene_id:MSH12.13~unknown pro... 39 0.004
BG449417 similar to GP|15810385|gb| unknown protein {Arabidopsis... 35 0.087
BG454151 34 0.11
TC91387 similar to GP|21537050|gb|AAM61391.1 putative phosphate/... 30 1.6
TC84572 weakly similar to PIR|C96566|C96566 hypothetical protein... 30 2.8
BQ135691 similar to PIR|E96636|E966 hypothetical protein T7P1.21... 30 2.8
TC79954 homologue to GP|9294548|dbj|BAB02811.1 ethylene responsi... 30 2.8
TC87142 similar to GP|17976705|emb|CAC80839. dihydrofolate synth... 29 3.7
BQ751580 29 3.7
TC86810 homologue to GP|6066285|gb|AAF03236.1| ubiquitin carrier... 29 4.8
TC88441 similar to GP|14194139|gb|AAK56264.1 AT3g06960/F17A9_11 ... 29 4.8
TC88271 similar to GP|23617258|dbj|BAC20925. hypothetical protei... 29 4.8
TC84866 28 6.3
AW695694 28 8.2
>TC87858 similar to GP|9757903|dbj|BAB08350.1 gene_id:MMN10.5~unknown
protein {Arabidopsis thaliana}, partial (34%)
Length = 2037
Score = 487 bits (1254), Expect(2) = 0.0
Identities = 241/245 (98%), Positives = 243/245 (98%)
Frame = +1
Query: 179 GEVETRSGSIGLAYGSGDARIRPFGTPYGKVDNTVLSIAESYNKDETNIISFGGFPDERG 238
G++ RSGSIGLAYGSGDARIRPFGTPYGKVDNTVLSIAESYNKDETNIISFGGFPDERG
Sbjct: 754 GKLRRRSGSIGLAYGSGDARIRPFGTPYGKVDNTVLSIAESYNKDETNIISFGGFPDERG 933
Query: 239 VISVGRAATDYEQLYNQSSVHVSTTAHEKELEASNSDVVASTPLVTTKKPESVSKNKQDI 298
VISVGRAATDYEQLYNQSSVHVSTTAHEKELEASNSDVVASTPLVTTKKPESVSKNKQDI
Sbjct: 934 VISVGRAATDYEQLYNQSSVHVSTTAHEKELEASNSDVVASTPLVTTKKPESVSKNKQDI 1113
Query: 299 KSTRKESPNTFPTNVRSLISTGMLDGVPVKYVSVAREELRGIIKGTTYLCGCQSCNYAKG 358
KSTRKESPNTFPTNVRSLISTGMLDGVPVKYVSVAREELRGIIKGTTYLCGCQSCNYAKG
Sbjct: 1114KSTRKESPNTFPTNVRSLISTGMLDGVPVKYVSVAREELRGIIKGTTYLCGCQSCNYAKG 1293
Query: 359 LNAFEFEKHAGCKSKHPNNHIYFENGKTIYQIVQELRSTPESSLFDTIQTIFGAPINQKA 418
LNAFEFEKHAGCKSKHPNNHIYFENGKTIYQIVQELRSTPESSLFDTIQTIFGAPINQKA
Sbjct: 1294LNAFEFEKHAGCKSKHPNNHIYFENGKTIYQIVQELRSTPESSLFDTIQTIFGAPINQKA 1473
Query: 419 FRIWK 423
FRIWK
Sbjct: 1474FRIWK 1488
Score = 388 bits (996), Expect(2) = 0.0
Identities = 183/184 (99%), Positives = 184/184 (99%)
Frame = +3
Query: 1 MVKGSGHVSDREPAFDNPSKIEPKRPHQWLIDATEGDFLPNKKQAIEDANERSSSGFSNV 60
MVKGSGHVSDREPAFDNPSKIEPKRPHQWLIDATEGDFLPNKKQAIEDANERSSSGFSNV
Sbjct: 219 MVKGSGHVSDREPAFDNPSKIEPKRPHQWLIDATEGDFLPNKKQAIEDANERSSSGFSNV 398
Query: 61 NFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVNFTEKNTYVSGDGSDVRSKMIANHYGD 120
NFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVNFTEKNTYVSGDGSDVRSKMIANHYGD
Sbjct: 399 NFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVNFTEKNTYVSGDGSDVRSKMIANHYGD 578
Query: 121 GASFGLSISHSTEDSEPCMNFGGIKKVKVNQVNPSDVQAPEQHNFDRQSTGDLHHVYHGE 180
GASFGLSISHSTEDSEPCMNFGGIKKVKVNQVNPSDVQAPEQHNFDRQSTGDLHHVYHGE
Sbjct: 579 GASFGLSISHSTEDSEPCMNFGGIKKVKVNQVNPSDVQAPEQHNFDRQSTGDLHHVYHGE 758
Query: 181 VETR 184
VET+
Sbjct: 759 VETK 770
Score = 72.4 bits (176), Expect = 4e-13
Identities = 33/34 (97%), Positives = 34/34 (99%)
Frame = +2
Query: 467 QNHFKQQHASFNGFMEMKNVTSKSPPSLRKLNLG 500
+NHFKQQHASFNGFMEMKNVTSKSPPSLRKLNLG
Sbjct: 1487 KNHFKQQHASFNGFMEMKNVTSKSPPSLRKLNLG 1588
>AW774768 similar to GP|3236235|gb|A unknown protein {Arabidopsis thaliana},
partial (10%)
Length = 665
Score = 140 bits (354), Expect = 9e-34
Identities = 64/144 (44%), Positives = 99/144 (68%), Gaps = 7/144 (4%)
Frame = +2
Query: 297 DIKSTRKESPNTFPTNVRSLISTGMLDGVPVKYV-SVAREELRGIIKGTTYLCGCQSCNY 355
++K ++K PN FP NV+ L+STG+LDG VKY+ + + EL GII YLCGC C+Y
Sbjct: 59 ELKMSKKVVPNAFPNNVKKLLSTGILDGAAVKYIYNPGKVELDGIIGDGGYLCGCSMCSY 238
Query: 356 AKGLNAFEFEKHAGCKSKHPNNHIYFENGKTIYQIVQELRSTPESSLFDTIQTIFGAPIN 415
++ L+A+EFE+HAG K++HPNNHI+ ENGK IY I+ E+++ S+ + I+ + G+ IN
Sbjct: 239 SRVLSAYEFEQHAGAKTRHPNNHIFLENGKPIYSIIHEIKTATNSTPDEVIKNVAGSSIN 418
Query: 416 QKAFRIWK------GKEIPNLFKH 433
+ +F++WK K++P K+
Sbjct: 419 EGSFQVWKESLLQSNKKVPTQKKY 490
>TC88101 similar to PIR|B86182|B86182 hypothetical protein [imported] -
Arabidopsis thaliana, partial (7%)
Length = 1362
Score = 69.7 bits (169), Expect = 2e-12
Identities = 36/113 (31%), Positives = 60/113 (52%), Gaps = 6/113 (5%)
Frame = +3
Query: 305 SPNTFPTNVRSLISTGMLDGVPVKYVSVAR------EELRGIIKGTTYLCGCQSCNYAKG 358
S FP ++ L++TG+L+G+ V+YV + ++L+G+IK LC C SC +
Sbjct: 327 SGKRFPVKLKDLLATGILEGLKVRYVKGQKARKPGEKDLQGVIKDAGILCFCGSCKGNQV 506
Query: 359 LNAFEFEKHAGCKSKHPNNHIYFENGKTIYQIVQELRSTPESSLFDTIQTIFG 411
++ FE HAG +K P + Y EN K + ++ S P +L +Q + G
Sbjct: 507 VSPNVFELHAGSANKRPPEYTYLENRKLLRDVMNACSSLPLDTLDKVVQMVLG 665
>BF645514
Length = 566
Score = 62.4 bits (150), Expect = 4e-10
Identities = 45/130 (34%), Positives = 63/130 (47%), Gaps = 3/130 (2%)
Frame = +3
Query: 1 MVKGSGHVSDREP-AFDNPSKIEPKRPHQWLIDATEGDFLPNKKQAIEDANERSSSGFSN 59
M K SG+++D + ++N S++E KR +Q +D E D PNKKQA+E N SSG +
Sbjct: 192 MGKSSGNLNDSDDMTYENSSRLESKRSNQSFMDGLEVDLFPNKKQAVEAPNS-LSSGMLS 368
Query: 60 VNFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVNFTEKNT--YVSGDGSDVRSKMIANH 117
N W N HS H +LF +NF + N + K I +
Sbjct: 369 SNILSW-GNSTLHSLTGH---FTSQLFDPGAAIMNFEDTNVCPLTIDNKLSAERKDILDP 536
Query: 118 YGDGASFGLS 127
+G ASFGLS
Sbjct: 537 FGGDASFGLS 566
>BE239959 similar to GP|9758032|dbj| gene_id:MSH12.13~unknown protein
{Arabidopsis thaliana}, partial (8%)
Length = 322
Score = 39.3 bits (90), Expect = 0.004
Identities = 16/27 (59%), Positives = 20/27 (73%)
Frame = +2
Query: 397 TPESSLFDTIQTIFGAPINQKAFRIWK 423
+P+ LFD IQT+ GA INQ+ FR WK
Sbjct: 2 SPQEMLFDAIQTVTGATINQRNFRTWK 82
>BG449417 similar to GP|15810385|gb| unknown protein {Arabidopsis thaliana},
partial (13%)
Length = 630
Score = 34.7 bits (78), Expect = 0.087
Identities = 35/121 (28%), Positives = 52/121 (42%), Gaps = 5/121 (4%)
Frame = +2
Query: 256 SSVHVSTTAHEKELEASNSDVVASTPLVTTKKPESVSKNKQDIKSTRKESPNTFPTNVRS 315
SSV T HE LEA S V ++ +TT E+ S ++ K++++E P
Sbjct: 182 SSVSDEETKHEGVLEAGKSHGVQASSSLTTS--EAKSNEAKEEKNSKQEEP--------- 328
Query: 316 LISTGMLDGVPVK-YVSVAREELRGIIKGTTYLCGCQSCNYAKGL----NAFEFEKHAGC 370
++ +P K Y+ V +E T Y ++CNYAKG N GC
Sbjct: 329 --KIDLVINIPQKSYIPVRKESWN---NATEY----KACNYAKGKWVPDNKHPLYSXFGC 481
Query: 371 K 371
K
Sbjct: 482 K 484
>BG454151
Length = 598
Score = 34.3 bits (77), Expect = 0.11
Identities = 31/104 (29%), Positives = 48/104 (45%), Gaps = 1/104 (0%)
Frame = +3
Query: 256 SSVHVSTTAHEKELEASNSDVVASTPLVTTKKPESVSKNKQDIKSTRKESPNTFPTNVRS 315
SSV T HE LEA S V ++ +TT E+ S ++ K++++E P
Sbjct: 342 SSVSDEETKHEGVLEAGKSHGVQASSSLTTS--EAKSNEAKEEKNSKQEEP--------- 488
Query: 316 LISTGMLDGVPVK-YVSVAREELRGIIKGTTYLCGCQSCNYAKG 358
++ +P K Y+ V +E T Y ++CNYAKG
Sbjct: 489 --KIDLVINIPQKSYIPVRKESWN---NATEY----KACNYAKG 593
>TC91387 similar to GP|21537050|gb|AAM61391.1 putative
phosphate/phosphoenolpyruvate translocator {Arabidopsis
thaliana}, partial (23%)
Length = 680
Score = 30.4 bits (67), Expect = 1.6
Identities = 28/121 (23%), Positives = 47/121 (38%), Gaps = 17/121 (14%)
Frame = +2
Query: 356 AKGLNAFEFEKHAGCKSKHPNNHIY------FENGKTIYQIVQELRST------PESSLF 403
+ LN + H +NHI F+N + LR T P SS F
Sbjct: 17 SSNLNMHNLYVSPSSSTLHLSNHITKPKFHPFQNSHSHNFSSSPLRLTIDGFACPSSSFF 196
Query: 404 DTI---QTIFGAPINQKAFRIWKGKEIPNLFKH--KQTNGCIGNVEFYCLGFVWYVINPL 458
T+ F + +FR++ +P KQT+G + +++ + WY++N
Sbjct: 197 QTVGKSSPFFISNPKMDSFRVFAASSVPEAQSDEGKQTSGLVQSLQLGFMFATWYLLNIY 376
Query: 459 F 459
F
Sbjct: 377 F 379
>TC84572 weakly similar to PIR|C96566|C96566 hypothetical protein F6D8.22
[imported] - Arabidopsis thaliana, partial (55%)
Length = 657
Score = 29.6 bits (65), Expect = 2.8
Identities = 21/81 (25%), Positives = 32/81 (38%), Gaps = 8/81 (9%)
Frame = +1
Query: 24 KRPHQWLIDATEGDFLPNKKQ--------AIEDANERSSSGFSNVNFTPWENNHNFHSDP 75
+R +WL + DFLP + A+ E + F+N+N TPW +
Sbjct: 1 RRGRRWLSRNVDRDFLPAPFELFPSGLGNALMQVTENINKLFNNMNLTPWSLSGRVKESD 180
Query: 76 SHQNQLIDRLFGSETRPVNFT 96
+H D + G VN T
Sbjct: 181NHYKLKYD-MPGIPKENVNIT 240
>BQ135691 similar to PIR|E96636|E966 hypothetical protein T7P1.21 [imported]
- Arabidopsis thaliana, partial (4%)
Length = 1215
Score = 29.6 bits (65), Expect = 2.8
Identities = 17/51 (33%), Positives = 25/51 (48%)
Frame = -2
Query: 22 EPKRPHQWLIDATEGDFLPNKKQAIEDANERSSSGFSNVNFTPWENNHNFH 72
+ K HQ AT LP + DA + S+ SN++ P N+H+FH
Sbjct: 527 QAKPAHQTTQPATPPYKLPPRPLKRNDAPDHSAH*LSNIDPPPIPNHHSFH 375
>TC79954 homologue to GP|9294548|dbj|BAB02811.1 ethylene responsive element
binding factor-like protein {Arabidopsis thaliana},
partial (11%)
Length = 520
Score = 29.6 bits (65), Expect = 2.8
Identities = 22/75 (29%), Positives = 31/75 (41%), Gaps = 4/75 (5%)
Frame = -1
Query: 66 ENNHNFHSDPSHQNQLIDRLFGSETRPVNFTEKNTYVSGDGSDVRSK----MIANHYGDG 121
+ NHNF P H+ +I T + T S +G +RS + D
Sbjct: 295 KKNHNFFHKPIHEVHIIFHDQRHRTVQCRSSPPATSASSNGGALRSNGRGARLNESITDE 116
Query: 122 ASFGLSISHSTEDSE 136
A+ LS S +TED E
Sbjct: 115 AALSLSPSSTTEDDE 71
>TC87142 similar to GP|17976705|emb|CAC80839. dihydrofolate synthetase
/folylpolyglutamate synthetase {Arabidopsis thaliana},
partial (38%)
Length = 832
Score = 29.3 bits (64), Expect = 3.7
Identities = 29/119 (24%), Positives = 45/119 (37%), Gaps = 2/119 (1%)
Frame = +1
Query: 35 EGDFLPNKKQAIEDANERSSSGFSNVNFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVN 94
+ L K +E ++ ++G + + W N N H + SH Q+ T P
Sbjct: 193 DAKLLNGLKLGLEGEHQYVNAGLAVALCSTWMNI-NGHLEESHLKQI------QHTLPEQ 351
Query: 95 FTEKNTYVS--GDGSDVRSKMIANHYGDGASFGLSISHSTEDSEPCMNFGGIKKVKVNQ 151
F T S G VR + + N + F L +HS E E C + + NQ
Sbjct: 352 FINGLTTASLQGRAQIVRDEHVNNKISNELVFFLDGAHSPESMEACAKWFSLAIKDQNQ 528
>BQ751580
Length = 183
Score = 29.3 bits (64), Expect = 3.7
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = -2
Query: 39 LPNKKQAIEDANERSSSGFSNVNFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVN 94
L NK ++ E ++SG S +H+DPS N I+R + PVN
Sbjct: 155 LHNKDRSYETVTSAAASGSS-------AEQAGYHTDPSSDNSSIERAHPHKPEPVN 9
>TC86810 homologue to GP|6066285|gb|AAF03236.1| ubiquitin carrier protein 4
{Glycine max}, complete
Length = 857
Score = 28.9 bits (63), Expect = 4.8
Identities = 15/49 (30%), Positives = 26/49 (52%)
Frame = +3
Query: 142 GGIKKVKVNQVNPSDVQAPEQHNFDRQSTGDLHHVYHGEVETRSGSIGL 190
GG+ KV+V + P+ + + S G ++ +YH V+ SGS+ L
Sbjct: 243 GGVWKVRV--------ELPDAYPYKSPSIGFINKIYHPNVDEMSGSVCL 365
>TC88441 similar to GP|14194139|gb|AAK56264.1 AT3g06960/F17A9_11
{Arabidopsis thaliana}, partial (47%)
Length = 1798
Score = 28.9 bits (63), Expect = 4.8
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +1
Query: 386 TIYQIVQELRSTPESSLFDTIQTIFGAPIN 415
T++ +V++L TP + +F + +FG P+N
Sbjct: 418 TVFLLVEKLWETPTTEIFVCLGIMFGVPVN 507
>TC88271 similar to GP|23617258|dbj|BAC20925. hypothetical protein~predicted
by FGENESH etc. {Oryza sativa (japonica
cultivar-group)}, partial (31%)
Length = 1637
Score = 28.9 bits (63), Expect = 4.8
Identities = 29/122 (23%), Positives = 44/122 (35%), Gaps = 3/122 (2%)
Frame = +1
Query: 82 IDRLFGSE---TRPVNFTEKNTYVSGDGSDVRSKMIANHYGDGASFGLSISHSTEDSEPC 138
ID L +E T V F + N SG D + T+DS+
Sbjct: 625 IDGLVENEDDITDEVEFDDNNASTSGSVPDASDDSV-----------------TDDSQKS 753
Query: 139 MNFGGIKKVKVNQVNPSDVQAPEQHNFDRQSTGDLHHVYHGEVETRSGSIGLAYGSGDAR 198
N + Q S+V+ P+ H+F RQ +L GSI G+ ++
Sbjct: 754 PNHSDKITEPLGQNKESEVREPQNHSFTRQEQSELF-----------GSISSPKGNKESE 900
Query: 199 IR 200
+R
Sbjct: 901 VR 906
>TC84866
Length = 913
Score = 28.5 bits (62), Expect = 6.3
Identities = 25/88 (28%), Positives = 37/88 (41%)
Frame = +2
Query: 49 ANERSSSGFSNVNFTPWENNHNFHSDPSHQNQLIDRLFGSETRPVNFTEKNTYVSGDGSD 108
A+ R+SS T N + SDP +Q + VN EKNT + D S
Sbjct: 113 ASSRNSSIVGESTQTCVNNEPDRQSDPEKFDQDVS---------VN-DEKNTKILSDSSQ 262
Query: 109 VRSKMIANHYGDGASFGLSISHSTEDSE 136
+ + +H GD +S S+ +SE
Sbjct: 263 MHFDVAQSHLGDKGVVSSPLSASSMESE 346
>AW695694
Length = 649
Score = 28.1 bits (61), Expect = 8.2
Identities = 17/45 (37%), Positives = 20/45 (43%), Gaps = 4/45 (8%)
Frame = +1
Query: 314 RSLISTGMLDGVPVKYVSVARE----ELRGIIKGTTYLCGCQSCN 354
RS + L PV YV +E L G+I G LC C CN
Sbjct: 358 RSFLILVFLMVCPVVYVGCKKEAINSRLEGVIAGGGILCSCCLCN 492
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.132 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,876,799
Number of Sequences: 36976
Number of extensions: 230282
Number of successful extensions: 1030
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1019
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1028
length of query: 500
length of database: 9,014,727
effective HSP length: 100
effective length of query: 400
effective length of database: 5,317,127
effective search space: 2126850800
effective search space used: 2126850800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC139841.9