BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139841.5 + phase: 0
(252 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85305 aquaporin 371 e-103
TC77711 similar to PIR|T46160|T46160 caffeic acid O-methyltransf... 308 1e-84
TC77007 similar to PIR|T10804|T10804 tonoplast intrinsic protein... 301 2e-82
TC78780 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein... 293 3e-80
TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic prot... 291 1e-79
TC77651 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein... 288 2e-78
BI266516 similar to PIR|JQ2288|JQ2 SPCP2 protein - soybean, part... 278 8e-76
TC77008 similar to PIR|A84653|A84653 hypothetical protein At2g25... 265 2e-71
TC76539 aquaporin [Medicago truncatula] 144 2e-35
TC78388 homologue to GP|8071620|gb|AAF71816.1| putative aquapori... 142 9e-35
AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein... 138 2e-33
TC85260 homologue to GP|3158476|gb|AAC17529.1| aquaporin 2 {Sama... 134 3e-32
TC85263 homologue to GP|5139539|emb|CAB45651.1 putative plasma m... 128 2e-30
TC89494 homologue to GP|13447793|gb|AAK26754.1 plasma membrane i... 125 2e-29
TC88670 similar to GP|535780|dbj|BAA05654.1| transmembrane prote... 124 5e-29
TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembr... 124 5e-29
TC76601 aquaporin-like protein PIP2 [Medicago truncatula] 120 4e-28
AA660249 homologue to PIR|T09297|T092 tonoplast intrinsic protei... 102 1e-22
TC86110 multifunctional aquaporin [Medicago truncatula] 102 2e-22
TC77955 similar to GP|5139541|emb|CAB45652.1 nodulin26-like majo... 101 2e-22
>TC85305 aquaporin
Length = 1221
Score = 371 bits (953), Expect = e-103
Identities = 188/252 (74%), Positives = 208/252 (81%)
Frame = +2
Query: 1 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 60
MPI IA+G P E D LKA LAEFIS IFVFAG GSG+AYNKLTN+GAATPAGL++
Sbjct: 98 MPIRNIAVGTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 277
Query: 61 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 120
AS++HAFALFVAVSVGANISGGHVNPAVTFGAF+GG+ITL+RG++Y IAQLLGS+VA L
Sbjct: 278 ASIAHAFALFVAVSVGANISGGHVNPAVTFGAFVGGNITLLRGIVYIIAQLLGSIVASAL 457
Query: 121 LKIATGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIG 180
L T AF LS GVG ALV EIVMTFGLVYTVYATAVDPK G++G IAPIAIG
Sbjct: 458 LVFVTAS-SVPAFGLSEGVGVGPALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIG 634
Query: 181 FIVGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFIT 240
FIVGANIL GGAF GASMNPAVSFGPAVVSW+W+NHWVYW GPLIG IA +VYE FI
Sbjct: 635 FIVGANILVGGAFTGASMNPAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEVLFIN 814
Query: 241 PSSYEQLPVTDY 252
S++EQLP TDY
Sbjct: 815 -STHEQLPTTDY 847
>TC77711 similar to PIR|T46160|T46160 caffeic acid O-methyltransferase-like
protein - Arabidopsis thaliana, partial (86%)
Length = 2361
Score = 308 bits (790), Expect = 1e-84
Identities = 158/242 (65%), Positives = 184/242 (75%)
Frame = +2
Query: 6 IAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSH 65
IA G + +++A +AEFIS LIFVFAG GS +AY KLT+ A PAGLVA ++ H
Sbjct: 80 IAFGRFDDSFSVSSIRAYVAEFISTLIFVFAGVGSAIAYAKLTSGAALDPAGLVAVAVCH 259
Query: 66 AFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIAT 125
FALFVAVSVGANISGGHVNPAVTFG IGG IT++ G+ YWIAQLLGS+VAC LLK AT
Sbjct: 260 GFALFVAVSVGANISGGHVNPAVTFGLAIGGQITILTGIFYWIAQLLGSIVACFLLKYAT 439
Query: 126 GGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGA 185
GGL S++SGVGA +V EI++TFGLVYTVYATA DPK GSLGTIAPIAIG IVGA
Sbjct: 440 GGLTIPIHSVASGVGAGEGVVTEIIITFGLVYTVYATAADPKKGSLGTIAPIAIGLIVGA 619
Query: 186 NILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFITPSSYE 245
NILA G F G SMNPA SFGPAV+S + N+W+YWVGPLIG +A V+Y F+ PS +
Sbjct: 620 NILAAGPFSGGSMNPARSFGPAVLSGDYHNNWIYWVGPLIGGGLAGVIYSYVFM-PSDHV 796
Query: 246 QL 247
L
Sbjct: 797 PL 802
>TC77007 similar to PIR|T10804|T10804 tonoplast intrinsic protein delta
type - upland cotton, complete
Length = 1041
Score = 301 bits (770), Expect = 2e-82
Identities = 147/239 (61%), Positives = 183/239 (76%)
Frame = +2
Query: 1 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 60
+ ++ IA G + ++KA +AEFIS L+FVFAG GS +AY KLT++ A PAGL+A
Sbjct: 71 LKMAGIAFGRLDDSFSFGSIKAYIAEFISTLLFVFAGVGSAIAYGKLTSDAALDPAGLLA 250
Query: 61 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 120
++ H FALFVAV+VGANISGGHVNPAVTFG +GG IT++ G+ YWIAQLLGS+VAC L
Sbjct: 251 VAVCHGFALFVAVAVGANISGGHVNPAVTFGLAVGGQITILTGIFYWIAQLLGSIVACFL 430
Query: 121 LKIATGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIG 180
L+ TGGLET S+++ VG +V EI++TFGLVYTVYATA DPK GS+GTIAPIAIG
Sbjct: 431 LQFVTGGLETPIHSVAAEVGPIGGVVTEIIITFGLVYTVYATAADPKKGSIGTIAPIAIG 610
Query: 181 FIVGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFI 239
FIVGANILA G F G SMNPA SFGPAVVS + ++W+YW GPLIG +A ++Y F+
Sbjct: 611 FIVGANILAAGPFSGGSMNPARSFGPAVVSGNFHDNWIYWAGPLIGGGLAGLIYGNVFM 787
>TC78780 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein alpha -
kidney bean, partial (95%)
Length = 999
Score = 293 bits (751), Expect = 3e-80
Identities = 140/255 (54%), Positives = 184/255 (71%), Gaps = 3/255 (1%)
Frame = +1
Query: 1 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 60
M R A G E D+++A +AEF S IFVFAGEGSG+A K+ + A + L+A
Sbjct: 19 MATRRYAFGRVDEAYHPDSIRATIAEFASTFIFVFAGEGSGLALVKIYQDSAFSAGELLA 198
Query: 61 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 120
+L+HAFALF AVS ++SGGHVNPAVTFGA IGG I+++R + YWIAQLLG++VA LL
Sbjct: 199 VALAHAFALFAAVSSSMHVSGGHVNPAVTFGALIGGRISVLRAVYYWIAQLLGAIVAALL 378
Query: 121 LKIATGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIG 180
L++ T + F L+ G+G + L+ EI+MTFGL+YTVYATA+DPK GS+G IAP+AIG
Sbjct: 379 LRLVTNNMRPGGFHLARGIGVGHGLILEIIMTFGLMYTVYATAIDPKRGSIGAIAPLAIG 558
Query: 181 FIVGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFI- 239
IVGANIL GG FDGA MNPA++FGP++V W W HW++WVGP IG+A+AA++YE I
Sbjct: 559 LIVGANILVGGPFDGACMNPALAFGPSLVGWRWHYHWIFWVGPFIGAALAALIYEYLVIP 738
Query: 240 --TPSSYEQLPVTDY 252
P +++ L DY
Sbjct: 739 TEPPHAHQPLAPEDY 783
>TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (97%)
Length = 1055
Score = 291 bits (746), Expect = 1e-79
Identities = 148/248 (59%), Positives = 184/248 (73%)
Frame = +1
Query: 5 RIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLS 64
+I G + A +LKA L+EFI+ LIFVFAG GS +AYN LT++ A PAGLVA +++
Sbjct: 118 KIGFGTFDDSFSAASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVA 297
Query: 65 HAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIA 124
HAFALFV V++ ANISGGH+NPAVTFG IGG+IT++ GL YWIAQLLGS+VA LLL
Sbjct: 298 HAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYV 477
Query: 125 TGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVG 184
T +++G+ LVFEI++TFGLVYTVYATA DPK GS+GTIAPIAIGF+VG
Sbjct: 478 TAK-SVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVG 654
Query: 185 ANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFITPSSY 244
ANILA G F G SMNPA SFGPAVVS +A++W+YWVGPLIG +A ++Y FI SY
Sbjct: 655 ANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFI--GSY 828
Query: 245 EQLPVTDY 252
P +++
Sbjct: 829 TPAPASEF 852
>TC77651 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein alpha -
kidney bean, partial (98%)
Length = 1154
Score = 288 bits (736), Expect = 2e-78
Identities = 133/239 (55%), Positives = 176/239 (72%)
Frame = +3
Query: 1 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 60
M R A G E D+++A +AEF S IFVFAGEGS +A K+ + A+ L+
Sbjct: 126 MATRRYAFGRMDEANHPDSIRATVAEFFSTCIFVFAGEGSALALRKIYKDAGASSGELLV 305
Query: 61 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 120
+++HAF+LF A+S ++SGGH+NPAVTFGA +GG I+++R L YW+AQLLGS VA LL
Sbjct: 306 LAIAHAFSLFAAISSSMHVSGGHINPAVTFGALLGGRISVLRALYYWVAQLLGSFVAALL 485
Query: 121 LKIATGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIG 180
L++ T + AF+++ GVGA NALV EI MTFGL+Y VYATA+DPK G++GTIAP+AI
Sbjct: 486 LRLVTNNMRPQAFNVAFGVGAGNALVLEIAMTFGLMYVVYATAIDPKRGTIGTIAPLAIA 665
Query: 181 FIVGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFI 239
+VGANILAGG FDGA MNPA +FGPA+V W W HW++WVGP +G+AIAA++YE +
Sbjct: 666 LVVGANILAGGPFDGACMNPARAFGPALVGWRWHYHWIFWVGPFLGAAIAALLYEYIMV 842
>BI266516 similar to PIR|JQ2288|JQ2 SPCP2 protein - soybean, partial (56%)
Length = 549
Score = 278 bits (711), Expect(2) = 8e-76
Identities = 144/144 (100%), Positives = 144/144 (100%)
Frame = +2
Query: 1 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 60
MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA
Sbjct: 83 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 262
Query: 61 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 120
ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL
Sbjct: 263 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 442
Query: 121 LKIATGGLETSAFSLSSGVGATNA 144
LKIATGGLETSAFSLSSGVGATNA
Sbjct: 443 LKIATGGLETSAFSLSSGVGATNA 514
Score = 33.9 bits (76), Expect = 0.061
Identities = 17/60 (28%), Positives = 30/60 (49%), Gaps = 1/60 (1%)
Frame = +2
Query: 175 APIAIGFIVGANILAGGAFDGASMNPAVSFGPAVVSW-TWANHWVYWVGPLIGSAIAAVV 233
A ++ F + + G G +NPAV+FG + T +YW+ L+GS +A ++
Sbjct: 263 ASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLL 442
Score = 23.1 bits (48), Expect(2) = 8e-76
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +3
Query: 145 LVFEIVMTFGL 155
LVFEI MTFGL
Sbjct: 516 LVFEIXMTFGL 548
>TC77008 similar to PIR|A84653|A84653 hypothetical protein At2g25810
[imported] - Arabidopsis thaliana, partial (97%)
Length = 967
Score = 265 bits (676), Expect = 2e-71
Identities = 128/237 (54%), Positives = 169/237 (71%)
Frame = +2
Query: 3 ISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAAS 62
+++IA+G E + D ++A + EFI+ +FVFAG GS M +KL+ + GL +
Sbjct: 23 MAKIALGTTREATQPDCIQALIVEFIATFLFVFAGVGSAMTADKLSGDALV---GLFFVA 193
Query: 63 LSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLK 122
++HA + V +S A+ISGGH+NPAVT G +GGHIT++R +LYWI QL+ S AC LL
Sbjct: 194 IAHALVVAVMISA-AHISGGHLNPAVTLGLLVGGHITIVRSILYWIDQLIASAAACYLLH 370
Query: 123 IATGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFI 182
+GGL T A +L+SGVG T +V+EIV+TF L++TVYAT VDPK G+L + P +GF+
Sbjct: 371 YLSGGLTTPAHTLASGVGYTQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFV 550
Query: 183 VGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFI 239
VGANILAGGAF ASMNPA SFGPA+VS W +HWVYWVGPLIG +A +YE FFI
Sbjct: 551 VGANILAGGAFSAASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENFFI 721
>TC76539 aquaporin [Medicago truncatula]
Length = 1423
Score = 144 bits (364), Expect = 2e-35
Identities = 92/255 (36%), Positives = 139/255 (54%), Gaps = 20/255 (7%)
Frame = +1
Query: 13 EFGKADALKAALAEFISMLIFVFAGEGSGMAYNK-----LTNNGAATPAGLVAASLSHAF 67
E K +A +AEF++ L+F++ + + Y++ + N G++ + +
Sbjct: 247 ELTKWSLYRAVIAEFVATLLFLYITVLTIIGYSRQSDTTIKGNTECDGVGVLGIAWAFGG 426
Query: 68 ALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGG 127
+F+ V A ISGGH+NPAVTFG F+G ++LIR LY +AQ LG++ L K G
Sbjct: 427 MIFILVYCTAGISGGHINPAVTFGLFVGRKVSLIRAFLYIVAQCLGAICGAGLAK----G 594
Query: 128 LETSAF--------SLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIA 175
+TS + ++S G AL EI+ TF LVYTV+ +A DPK + + +A
Sbjct: 595 FQTSYYNRYKGGVNTVSDGYSKGTALGAEIIGTFVLVYTVF-SATDPKRSARDSHVPVLA 771
Query: 176 PIAIGFIVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAV 232
P+ IGF V LA G +NPA SFGPAV+ W + W+YWVGP IG+A+AA
Sbjct: 772 PLPIGFAVFMVHLATIPVTGTGINPARSFGPAVIFNNEKAWDDQWIYWVGPFIGAAVAA- 948
Query: 233 VYETFFITPSSYEQL 247
+Y + + S+ + L
Sbjct: 949 IYHQYILRGSAIKAL 993
>TC78388 homologue to GP|8071620|gb|AAF71816.1| putative aquaporin PIP2-1
{Vitis berlandieri x Vitis rupestris}, partial (91%)
Length = 1120
Score = 142 bits (359), Expect = 9e-35
Identities = 90/239 (37%), Positives = 134/239 (55%), Gaps = 15/239 (6%)
Frame = +2
Query: 12 SEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTN---NGA-ATPAGLVAASLSHAF 67
+E G+ +A +AEF++ L+F++ + + YN T+ NG G++ + +
Sbjct: 119 AELGQWSFYRALIAEFVATLLFLYVTVLTVIGYNAQTDPAHNGTNCDGVGILGIAWAFGG 298
Query: 68 ALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGG 127
+FV V A ISGGH+NPAVTFG F+ ++LIR +LY +AQ LG++ L+K G
Sbjct: 299 MIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFQKG 478
Query: 128 ----LETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAPIAI 179
+ A LS+G L EI+ TF LVYTV+ +A DPK + + +AP+ I
Sbjct: 479 YYNRYKGGANMLSAGYSKGTGLGAEIIGTFVLVYTVF-SATDPKRNARDSHVPVLAPLPI 655
Query: 180 GFIVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVVYE 235
GF V LA G +NPA SFG AV+ W + W++WVGP IG+AIAA+ ++
Sbjct: 656 GFAVFMVHLATIPITGTGINPARSFGAAVIYNNEKAWDDQWIFWVGPFIGAAIAAIYHQ 832
>AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (43%)
Length = 691
Score = 138 bits (348), Expect = 2e-33
Identities = 74/154 (48%), Positives = 103/154 (66%)
Frame = -2
Query: 6 IAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSH 65
+AIG+P L A A ++ LIFVFAG GS +AYN LT++ A PAGLVA +++H
Sbjct: 639 MAIGHP--------LGATGARCVATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAH 484
Query: 66 AFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIAT 125
AFALFV V++ ANISGGH+NPAVTFG IGG+IT++ GL YWIAQLLGS+VA LLL T
Sbjct: 483 AFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLLLNYVT 304
Query: 126 GGLETSAFSLSSGVGATNALVFEIVMTFGLVYTV 159
+++G+ + + +++ L+ ++
Sbjct: 303 -AKSVPTHGVAAGLNPIARIRYSVLVPIWLLLSI 205
Score = 37.7 bits (86), Expect = 0.004
Identities = 33/111 (29%), Positives = 51/111 (45%), Gaps = 9/111 (8%)
Frame = -2
Query: 132 AFSLSSGVGATNA-LVFEIVMTFGLVYTVYA-------TAVDPKNGSLGTIAPIAIGFIV 183
A ++ +GAT A V ++ F V + A A+DP G + A V
Sbjct: 642 AMAIGHPLGATGARCVATLIFVFAGVGSAIAYNDLTSDAALDPA-GLVAVAVAHAFALFV 466
Query: 184 GANILAGGAFDGASMNPAVSFGPAV-VSWTWANHWVYWVGPLIGSAIAAVV 233
G I A G +NPAV+FG A+ + T YW+ L+GS +A+++
Sbjct: 465 GVAIAAN--ISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVASLL 319
>TC85260 homologue to GP|3158476|gb|AAC17529.1| aquaporin 2 {Samanea saman},
complete
Length = 1282
Score = 134 bits (338), Expect = 3e-32
Identities = 94/254 (37%), Positives = 135/254 (53%), Gaps = 19/254 (7%)
Frame = +3
Query: 13 EFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPA----GLVAASLSHAFA 68
E K +A +AEFI+ L+F++ + + Y+ T+ A A G++ + +
Sbjct: 177 ELTKWSFYRALIAEFIATLLFLYVTVLTVIGYSIQTDVKAGGDACGGVGILGIAWAFGGM 356
Query: 69 LFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIAT--- 125
+FV V A ISGGH+NPAVTFG F+ ++LIR ++Y +AQ LG++ L+K
Sbjct: 357 IFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAIMYMVAQCLGAIAGVGLVKAFQSAY 536
Query: 126 -----GGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAP 176
GG S+GVG L EIV TF LVYTV+ +A DPK + + +AP
Sbjct: 537 FDRYGGGANFLHDGYSTGVG----LGAEIVGTFVLVYTVF-SATDPKRSARDSHVPVLAP 701
Query: 177 IAIGFIVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVV 233
+ IGF V LA G +NPA S G AV+ W +HW++WVGP IG+AIAA
Sbjct: 702 LPIGFAVFMVHLATIPVTGTGINPARSLGSAVIYNKDKPWDDHWIFWVGPFIGAAIAA-F 878
Query: 234 YETFFITPSSYEQL 247
Y F + + + L
Sbjct: 879 YHQFILRAGAVKAL 920
>TC85263 homologue to GP|5139539|emb|CAB45651.1 putative plasma membrane
intrinsic protein {Pisum sativum}, complete
Length = 1359
Score = 128 bits (321), Expect = 2e-30
Identities = 86/254 (33%), Positives = 133/254 (51%), Gaps = 19/254 (7%)
Frame = +3
Query: 13 EFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATP----AGLVAASLSHAFA 68
E K +A +AEF++ L+F++ + + Y+ ++ A G++ + +
Sbjct: 138 ELTKWSFYRALIAEFVATLLFLYVTVLTIIGYSHQSDPKAGGTDCDGVGILGIAWAFGGM 317
Query: 69 LFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGGL 128
+F+ V A ISGGH+NPAVTFG F+G ++LIR + Y AQ G++ L K G
Sbjct: 318 IFILVYCTAGISGGHINPAVTFGLFVGRKVSLIRAVFYMAAQSAGAICGTGLAK----GF 485
Query: 129 ETSAFS--------LSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAP 176
+ + F + G AL EI+ TF LVYTV+ +A DPK + + +AP
Sbjct: 486 QKAYFDRYGGGANFVHDGYNKGTALGAEIIGTFVLVYTVF-SATDPKRNARDSHVPVLAP 662
Query: 177 IAIGFIVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVV 233
+ IGF V LA G +NPA SFG AV+ W + W++WVGP IG+A+AA +
Sbjct: 663 LPIGFAVFMVHLATIPVTGTGINPARSFGSAVIYNDGKIWDDQWIFWVGPFIGAAVAA-I 839
Query: 234 YETFFITPSSYEQL 247
Y + + S+ + L
Sbjct: 840 YHQYILRGSAIKAL 881
>TC89494 homologue to GP|13447793|gb|AAK26754.1 plasma membrane integral
protein ZmPIP1-3 {Zea mays}, partial (93%)
Length = 1132
Score = 125 bits (314), Expect = 2e-29
Identities = 80/237 (33%), Positives = 123/237 (51%), Gaps = 12/237 (5%)
Frame = +1
Query: 11 PSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALF 70
P E +A +AEF++ +F++ + M N+ T+ + G+ + S +F
Sbjct: 166 PGELKSWSFYRAGIAEFVATFLFLYITILTVMGVNRSTSK--CSSVGIQGIAWSFGGMIF 339
Query: 71 VAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGGLET 130
V A ISGGH+NPAVTFG F+ ++L R + Y Q LG++ ++K G
Sbjct: 340 ALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIGMQCLGAICGAGVVKGFEGNARY 519
Query: 131 SAFS-----LSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAPIAIGF 181
F ++ G + L EIV TF LVYTV+ +A D K + + +AP+ IGF
Sbjct: 520 ELFKGGANFVNPGYTKGDGLGAEIVGTFVLVYTVF-SATDAKRNARDSHVPILAPLPIGF 696
Query: 182 IVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVVYE 235
V LA G +NPA S G A++ + W +HW++WVGP IG+A+AAV ++
Sbjct: 697 AVFLVHLATIPITGTGINPARSLGAAIIFNRDFAWDDHWIFWVGPFIGAALAAVYHQ 867
>TC88670 similar to GP|535780|dbj|BAA05654.1| transmembrane protein
{Arabidopsis thaliana}, complete
Length = 1106
Score = 124 bits (310), Expect = 5e-29
Identities = 81/232 (34%), Positives = 123/232 (52%), Gaps = 17/232 (7%)
Frame = +1
Query: 21 KAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALFVAVSVGANIS 80
+A +AEF++ +F++ + M NK +N + G+ + + +F V A IS
Sbjct: 208 RAGIAEFVATFLFLYITVLTVMGVNKSSNK--CSSVGVQGIAWAFGGMIFALVYCTAGIS 381
Query: 81 GGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLK--------IATGGLET-- 130
GGH+NPAVTFG F+ ++L R L Y I Q LG++ ++K GG T
Sbjct: 382 GGHINPAVTFGLFLARKLSLTRTLFYIIMQCLGAICGAAVVKGFQPHQYERLGGGANTLN 561
Query: 131 SAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAPIAIGFIVGAN 186
+S+ G+GA EIV TF LVYTV+ +A D K + + +AP+ IGF V
Sbjct: 562 KGYSIGDGLGA------EIVGTFVLVYTVF-SATDAKRNARDSHVPILAPLPIGFAVFLV 720
Query: 187 ILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVVYE 235
LA G +NPA S G A++ W +HW++WVGP G+A+AA+ ++
Sbjct: 721 HLATIPITGTGINPARSLGAALIYNKDQAWDDHWIFWVGPFTGAALAALYHQ 876
>TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembrane channel
protein - alfalfa, complete
Length = 1502
Score = 124 bits (310), Expect = 5e-29
Identities = 84/245 (34%), Positives = 124/245 (50%), Gaps = 16/245 (6%)
Frame = +3
Query: 11 PSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALF 70
PSE +A +AEF++ +F++ + M N+ + G+ + S +F
Sbjct: 213 PSELTSWSFYRAGIAEFVATFLFLYITILTVMGVNR--SESKCKTVGIQGIAWSFGGMIF 386
Query: 71 VAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGGLET 130
V A ISGGH+NPAVTFG F+ ++L R + Y + Q LG++ ++K G E
Sbjct: 387 ALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYMVMQTLGAICGAGVVK----GFEG 554
Query: 131 SAFSLSSGVGAT---------NALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAPI 177
F + GA + L EIV TF LVYTV+ +A D K + + +AP+
Sbjct: 555 KTFYTNKNGGANFVAPGYTKGDGLGAEIVGTFVLVYTVF-SATDAKRSARDSHVPILAPL 731
Query: 178 AIGFIVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVVY 234
IGF V LA G +NPA S G A++ W +HW++WVGP IG+A+AA +Y
Sbjct: 732 PIGFAVFLVHLATIPITGTGINPARSLGAAIIFNKDLGWDDHWIFWVGPFIGAALAA-LY 908
Query: 235 ETFFI 239
T I
Sbjct: 909 HTVVI 923
>TC76601 aquaporin-like protein PIP2 [Medicago truncatula]
Length = 1497
Score = 120 bits (302), Expect = 4e-28
Identities = 79/236 (33%), Positives = 122/236 (51%), Gaps = 12/236 (5%)
Frame = +2
Query: 11 PSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALF 70
PSE +A +AEF++ +F++ + M + + G+ + + +F
Sbjct: 440 PSELTSWSFYRAGIAEFVATFLFLYITVLTVMGVVRADSK--CKTVGIQGIAWAFGGMIF 613
Query: 71 VAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLL-----KIAT 125
V A ISGGH+NPAVTFG F+ ++L R L Y + Q LG++ ++ K
Sbjct: 614 ALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFEGKTRF 793
Query: 126 GGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGS----LGTIAPIAIGF 181
G L+ A ++ G + L EIV TF LVYTV+ +A D K + + +AP+ IGF
Sbjct: 794 GDLKGGANFVAPGYTKGDGLGAEIVGTFILVYTVF-SATDAKRSARDSHVPILAPLPIGF 970
Query: 182 IVGANILAGGAFDGASMNPAVSFGPAVV---SWTWANHWVYWVGPLIGSAIAAVVY 234
V LA G +NPA S G A++ W +HW++WVGP IG+A+AA+ +
Sbjct: 971 AVFLVHLATIPITGTGINPARSLGAAIIFNKDLGWDDHWIFWVGPFIGAALAALYH 1138
>AA660249 homologue to PIR|T09297|T092 tonoplast intrinsic protein homolog
MSMCP1 - alfalfa, partial (30%)
Length = 275
Score = 102 bits (255), Expect = 1e-22
Identities = 52/76 (68%), Positives = 59/76 (77%)
Frame = +2
Query: 1 MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVA 60
MPI IA+ P E D LKA LAEFIS IFVFAG GSG+AYNKLTN+GAATPAGL++
Sbjct: 14 MPIXNIAVDTPQEATHPDTLKAGLAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLIS 193
Query: 61 ASLSHAFALFVAVSVG 76
AS++ ALFVAVSVG
Sbjct: 194ASIAXGVALFVAVSVG 241
>TC86110 multifunctional aquaporin [Medicago truncatula]
Length = 1411
Score = 102 bits (253), Expect = 2e-22
Identities = 73/218 (33%), Positives = 109/218 (49%), Gaps = 1/218 (0%)
Frame = +3
Query: 20 LKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALFVAVSVGANI 79
L+ +AE + +FAG S + NK +N P + L+ L V + +I
Sbjct: 180 LQKLIAEMVGTYFLIFAGCAS-IVVNKDNDNVVTLPGIAIVWGLT----LLVLIYSLGHI 344
Query: 80 SGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLK-IATGGLETSAFSLSSG 138
SG H NPAVT L++ Y AQLLG+ +A LK I +G + + +L SG
Sbjct: 345 SGAHFNPAVTIAFATTRRFPLLQVPAYISAQLLGATLASGTLKLIFSGAHDHFSGTLPSG 524
Query: 139 VGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFDGASM 198
A V E + TF L++T+ A D + ++G +A IAIG + N++ G GASM
Sbjct: 525 SNL-QAFVLEFITTFYLMFTISGVATDTR--AIGELAGIAIGSTLLLNVMIAGPVTGASM 695
Query: 199 NPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYET 236
NP + GPA V + W+Y + P++G+ A VY T
Sbjct: 696 NPVRTLGPAFVHNEYRGIWIYLLSPILGAIAGAWVYNT 809
>TC77955 similar to GP|5139541|emb|CAB45652.1 nodulin26-like major intrinsic
protein {Pisum sativum}, partial (97%)
Length = 1272
Score = 101 bits (252), Expect = 2e-22
Identities = 72/231 (31%), Positives = 104/231 (44%)
Frame = +1
Query: 17 ADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALFVAVSVG 76
A L+ +AE I +FAG S + NK N P S+ A+ V V
Sbjct: 244 ASFLQKLVAEVIGTYFLIFAGCASVLV-NKNNENVVTLPG----ISIVWGLAVMVLVYSL 408
Query: 77 ANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGGLETSAFSLS 136
+ISG H NPAVT L + Y AQ+ GS +A L++ G
Sbjct: 409 GHISGAHFNPAVTIAFASTKRFPLKQVPAYVAAQVFGSTLASGTLRLIFTGKHNQFVGTL 588
Query: 137 SGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFDGA 196
A V E ++TF L++ + A D N ++G +A IA+G V N++ G GA
Sbjct: 589 PAGSDLQAFVIEFIITFYLMFIISGVATD--NRAIGELAGIAVGSTVLLNVMFAGPITGA 762
Query: 197 SMNPAVSFGPAVVSWTWANHWVYWVGPLIGSAIAAVVYETFFITPSSYEQL 247
SMNPA S GPA++ + W+Y V P++G+ A VY T ++
Sbjct: 763 SMNPARSIGPALLHSEYRGIWIYLVSPILGAVAGAWVYNVIRYTDKPVREI 915
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,812,190
Number of Sequences: 36976
Number of extensions: 87953
Number of successful extensions: 648
Number of sequences better than 10.0: 93
Number of HSP's better than 10.0 without gapping: 568
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 579
length of query: 252
length of database: 9,014,727
effective HSP length: 94
effective length of query: 158
effective length of database: 5,538,983
effective search space: 875159314
effective search space used: 875159314
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC139841.5