BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139708.14 - phase: 0
(992 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78191 homologue to GP|13111324|dbj|BAB32793. 110 kDa protein H... 747 0.0
TC90315 homologue to GP|21929218|dbj|BAC06183. 110kDa protein HM... 462 e-130
TC80443 homologue to GP|21929218|dbj|BAC06183. 110kDa protein HM... 258 6e-69
TC78529 similar to GP|14030876|gb|AAK52082.1 nuclease {Nicotiana... 38 0.022
TC88497 similar to GP|11994617|dbj|BAB02754. contains similarity... 33 0.71
TC89551 similar to GP|17887381|gb|AAL40864.1 receptor protein ki... 32 1.6
>TC78191 homologue to GP|13111324|dbj|BAB32793. 110 kDa protein HMP {Pisum
sativum}, partial (84%)
Length = 2058
Score = 747 bits (1928), Expect(2) = 0.0
Identities = 377/384 (98%), Positives = 379/384 (98%)
Frame = +1
Query: 609 KETCSIAFAFSGVRCPGREEPYSDEAIALMRRRIMQRDVEIEVETVDRTGTFLGSLWESR 668
K+ + FAFSGVRCPGREEPYSDEAIALMRRRIM RDVEIEVETVDRTGTFLGSLWESR
Sbjct: 637 KKLAVLLFAFSGVRCPGREEPYSDEAIALMRRRIMHRDVEIEVETVDRTGTFLGSLWESR 816
Query: 669 ANGAVPLLEAGLAKLQTSFGSDRIPDLHVLEQAEQSAKSKKLKIWENYVEGEVVPSGANV 728
ANGAVPLLEAGLAKLQTSFGSDRIPDLHVLEQAEQSAKSKKLKIWENYVEGEVVPSGANV
Sbjct: 817 ANGAVPLLEAGLAKLQTSFGSDRIPDLHVLEQAEQSAKSKKLKIWENYVEGEVVPSGANV 996
Query: 729 ESKQQEVLKVTVTEVLGGGKFYVQTVGDQKIASIQNQLASLNLKDAPVIGAFNPKKGDIV 788
ESKQQEVLKVTVTEVLGGGKFYVQTVGDQKIASIQNQLASLNLKDAPVIGAFNPKKGDIV
Sbjct: 997 ESKQQEVLKVTVTEVLGGGKFYVQTVGDQKIASIQNQLASLNLKDAPVIGAFNPKKGDIV 1176
Query: 789 LCYFHADSSWYRAMVVNTPRGPVESSKDAFEVFYIDYGNQEVVPYSQLRPLDPSVSAAPG 848
LCYFHADSSWYRAMVVNTPRGPVESSKDAFEVFYIDYGNQEVVPYSQLRPLDPSVSAAPG
Sbjct: 1177 LCYFHADSSWYRAMVVNTPRGPVESSKDAFEVFYIDYGNQEVVPYSQLRPLDPSVSAAPG 1356
Query: 849 LAQLCSLAYIKLPNLEEDFGQEAAEYLSELTLSSGKEFRAMVEEKDTTGGKVKGQGTGPI 908
LAQLCSLAYIKLPNLEEDFGQEAAEYLSELTLSSGKEFRAMVEEKDTTGGKVKGQGTGPI
Sbjct: 1357 LAQLCSLAYIKLPNLEEDFGQEAAEYLSELTLSSGKEFRAMVEEKDTTGGKVKGQGTGPI 1536
Query: 909 IAVTLVAVDSEISVNAAMLQEGLARMEKRNRWDRTARKQALDNLEMFQGEARTARRGMWQ 968
IAVTLVAVDSEISVNAAMLQEGLARMEKRNRWDRTARKQALDNLEMFQGEARTARRGMWQ
Sbjct: 1537 IAVTLVAVDSEISVNAAMLQEGLARMEKRNRWDRTARKQALDNLEMFQGEARTARRGMWQ 1716
Query: 969 YGDIQSDDEDTAPPQRKAGGGRGK 992
YGDIQSDDEDTAPPQRKAGGGRGK
Sbjct: 1717 YGDIQSDDEDTAPPQRKAGGGRGK 1788
Score = 340 bits (872), Expect(2) = 0.0
Identities = 175/189 (92%), Positives = 178/189 (93%)
Frame = +3
Query: 428 RDEKPAPYAREAKEFLRTRLIGRQVNVQMEYSRKVGPVDGSAVPPGAVDSRVMDFGSVFV 487
+++ P P R K RLIGRQVNVQMEYSRKVGPVDGSAVPPGAVDSRVMDFGSVFV
Sbjct: 99 KNQLPMPVKR--KSS*EHRLIGRQVNVQMEYSRKVGPVDGSAVPPGAVDSRVMDFGSVFV 272
Query: 488 LSSGKADGDDAPSPAVPASQQTGLNVAELIIGRGFGTVIRHRDFEERSNFYDALLAAEAR 547
LSSGKADGDDAPSPAVPASQQTGLNVAELIIGRGFGTVIRHRDFEERSNFYDALLAAEAR
Sbjct: 273 LSSGKADGDDAPSPAVPASQQTGLNVAELIIGRGFGTVIRHRDFEERSNFYDALLAAEAR 452
Query: 548 AISGRKGIHSAKDPPVMHITDLITASAKKAKDFLPFLHRSRRVPAVVEYVFSGHRFKLLI 607
AISGRKGIHSAKDPPVMHITDLITASAKKAKDFLPFLHRSRRVPAVVEYVFSGHRFKLLI
Sbjct: 453 AISGRKGIHSAKDPPVMHITDLITASAKKAKDFLPFLHRSRRVPAVVEYVFSGHRFKLLI 632
Query: 608 PKETCSIAF 616
PKETCSIAF
Sbjct: 633 PKETCSIAF 659
Score = 102 bits (254), Expect = 7e-22
Identities = 48/48 (100%), Positives = 48/48 (100%)
Frame = +2
Query: 398 ADDSIPYGSPQAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRT 445
ADDSIPYGSPQAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRT
Sbjct: 2 ADDSIPYGSPQAERRVNLSSIRCPKMGNPRRDEKPAPYAREAKEFLRT 145
Score = 52.0 bits (123), Expect = 1e-06
Identities = 38/125 (30%), Positives = 64/125 (50%), Gaps = 6/125 (4%)
Frame = +1
Query: 274 DPFGADAKFFTEMRVLNRDVRIVLEGVDKFSNLIGSVYYPDGESAKDLALELVENGFAKY 333
+P+ +A R+++RDV I +E VD+ +GS++ ES + A+ L+E G AK
Sbjct: 694 EPYSDEAIALMRRRIMHRDVEIEVETVDRTGTFLGSLW----ESRANGAVPLLEAGLAKL 861
Query: 334 -VEWSANMMEDEAKKKLKAAELEAKKTRLRIWTNY-----VPPTSNSKAIHDQNFTGKVV 387
+ ++ + D L+ AE AK +L+IW NY VP +N ++ + V
Sbjct: 862 QTSFGSDRIPD--LHVLEQAEQSAKSKKLKIWENYVEGEVVPSGANVESKQQEVLKVTVT 1035
Query: 388 EVVSG 392
EV+ G
Sbjct: 1036EVLGG 1050
>TC90315 homologue to GP|21929218|dbj|BAC06183. 110kDa protein HMP {Pisum
sativum}, partial (62%)
Length = 719
Score = 462 bits (1190), Expect = e-130
Identities = 239/239 (100%), Positives = 239/239 (100%)
Frame = +3
Query: 125 KGEASPFLAELLRLEEQAKQEGLGRWSKVPGAAEASVRNLPPSALGDASNFDAMGLLAKN 184
KGEASPFLAELLRLEEQAKQEGLGRWSKVPGAAEASVRNLPPSALGDASNFDAMGLLAKN
Sbjct: 3 KGEASPFLAELLRLEEQAKQEGLGRWSKVPGAAEASVRNLPPSALGDASNFDAMGLLAKN 182
Query: 185 KGVPMEALVEQVRDGSTLRIYLLPEFQFVQVFVAGIQAPQMGRRAAPESVVVPEVTVDTT 244
KGVPMEALVEQVRDGSTLRIYLLPEFQFVQVFVAGIQAPQMGRRAAPESVVVPEVTVDTT
Sbjct: 183 KGVPMEALVEQVRDGSTLRIYLLPEFQFVQVFVAGIQAPQMGRRAAPESVVVPEVTVDTT 362
Query: 245 NGDVPAEPRAPLTSAQRLAVSASAAETSADPFGADAKFFTEMRVLNRDVRIVLEGVDKFS 304
NGDVPAEPRAPLTSAQRLAVSASAAETSADPFGADAKFFTEMRVLNRDVRIVLEGVDKFS
Sbjct: 363 NGDVPAEPRAPLTSAQRLAVSASAAETSADPFGADAKFFTEMRVLNRDVRIVLEGVDKFS 542
Query: 305 NLIGSVYYPDGESAKDLALELVENGFAKYVEWSANMMEDEAKKKLKAAELEAKKTRLRI 363
NLIGSVYYPDGESAKDLALELVENGFAKYVEWSANMMEDEAKKKLKAAELEAKKTRLRI
Sbjct: 543 NLIGSVYYPDGESAKDLALELVENGFAKYVEWSANMMEDEAKKKLKAAELEAKKTRLRI 719
>TC80443 homologue to GP|21929218|dbj|BAC06183. 110kDa protein HMP {Pisum
sativum}, partial (34%)
Length = 617
Score = 258 bits (660), Expect = 6e-69
Identities = 130/136 (95%), Positives = 134/136 (97%)
Frame = +1
Query: 1 MAATAAGNSAWYKAKVKAVPSGDCIVVVSVAANAKLGVLPEKSITLSSLIAPRLARRGGV 60
MAATAAGNSAWYKAKVKAVPSGDCIVVVSVAANAKLGVLPEKSITLSSLIAPRLARRGGV
Sbjct: 205 MAATAAGNSAWYKAKVKAVPSGDCIVVVSVAANAKLGVLPEKSITLSSLIAPRLARRGGV 384
Query: 61 DEPFAWESREFLRKLLIGKEITFRIDYTVPSINREFGTVFLGDKNVALLVVSQGWAKVRE 120
DEPFAWESREFLRKLLIGKEITFRIDYTVPSINREFGTVFLGDKNVALLVVSQGWAKVRE
Sbjct: 385 DEPFAWESREFLRKLLIGKEITFRIDYTVPSINREFGTVFLGDKNVALLVVSQGWAKVRE 564
Query: 121 QGQQKGEASPFLAELL 136
QGQQKG+ASPF +++
Sbjct: 565 QGQQKGKASPFFGKIV 612
Score = 46.6 bits (109), Expect = 5e-05
Identities = 27/81 (33%), Positives = 47/81 (57%), Gaps = 3/81 (3%)
Frame = +1
Query: 382 FTGKVVEVVSGDCVIVADDSIPYGSPQA---ERRVNLSSIRCPKMGNPRRDEKPAPYARE 438
+ KV V SGDC++V S+ + E+ + LSS+ P++ RR P+A E
Sbjct: 238 YKAKVKAVPSGDCIVVV--SVAANAKLGVLPEKSITLSSLIAPRLA--RRGGVDEPFAWE 405
Query: 439 AKEFLRTRLIGRQVNVQMEYS 459
++EFLR LIG+++ +++Y+
Sbjct: 406 SREFLRKLLIGKEITFRIDYT 468
>TC78529 similar to GP|14030876|gb|AAK52082.1 nuclease {Nicotiana tabacum},
partial (56%)
Length = 618
Score = 37.7 bits (86), Expect = 0.022
Identities = 43/193 (22%), Positives = 86/193 (44%), Gaps = 7/193 (3%)
Frame = +3
Query: 179 GLLAKNKGVPMEALVEQVRDGSTLRIYLLP----EFQFV--QVFVAGI-QAPQMGRRAAP 231
G+ + +P++A + V DG L +Y+ E V V +A + +A RR
Sbjct: 75 GVQYERHTLPVDA--KAVADGDGLTVYVSTADPRESSQVPSNVHIAAVHRAEARSRRNYQ 248
Query: 232 ESVVVPEVTVDTTNGDVPAEPRAPLTSAQRLAVSASAAETSADPFGADAKFFTEMRVLNR 291
E+ + + +D + E L R+ + A +A P+G +AK V +
Sbjct: 249 EADALHKQIIDAGYRMISFENGEILAKKYRIRLRGIDAPENAMPYGKEAKTELTKIVQGK 428
Query: 292 DVRIVLEGVDKFSNLIGSVYYPDGESAKDLALELVENGFAKYVEWSANMMEDEAKKKLKA 351
+R+++ G D++ L+G +Y + ++L L+ K + W + + + +L+
Sbjct: 429 SLRVLIYGEDQYQRLVGDIYC-NNIFVQELMLK-------KGLAW--HYAAYDKRPELET 578
Query: 352 AELEAKKTRLRIW 364
E EA+ R+ +W
Sbjct: 579 WEKEARAKRVGLW 617
>TC88497 similar to GP|11994617|dbj|BAB02754. contains similarity to kinesin
protein~gene_id:MGL6.9 {Arabidopsis thaliana}, partial
(26%)
Length = 1310
Score = 32.7 bits (73), Expect = 0.71
Identities = 22/79 (27%), Positives = 35/79 (43%)
Frame = +2
Query: 422 KMGNPRRDEKPAPYAREAKEFLRTRLIGRQVNVQMEYSRKVGPVDGSAVPPGAVDSRVMD 481
K GNPR+D+ P P + KE L T + + Y ++ V G + +V++
Sbjct: 284 KSGNPRKDQAPNPVPQSNKEVLSTSSLPDSACAEDVYYQR------QEVKTGDMGRKVIE 445
Query: 482 FGSVFVLSSGKADGDDAPS 500
+ S+ AD D PS
Sbjct: 446 NENSLYSSAAAADVDKQPS 502
>TC89551 similar to GP|17887381|gb|AAL40864.1 receptor protein kinase-like
protein {Capsicum annuum}, partial (63%)
Length = 1291
Score = 31.6 bits (70), Expect = 1.6
Identities = 18/47 (38%), Positives = 25/47 (52%), Gaps = 1/47 (2%)
Frame = +2
Query: 528 HRDFEERSNFYDALL-AAEARAISGRKGIHSAKDPPVMHITDLITAS 573
H + +SN+YDA+L E IS KG + +PP + DLI S
Sbjct: 149 HPNTASKSNYYDAILNGVEIFKISDSKGNLAGTNPPPPQVQDLIDPS 289
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.133 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,289,621
Number of Sequences: 36976
Number of extensions: 287870
Number of successful extensions: 1385
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1365
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1383
length of query: 992
length of database: 9,014,727
effective HSP length: 106
effective length of query: 886
effective length of database: 5,095,271
effective search space: 4514410106
effective search space used: 4514410106
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC139708.14