BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138580.5 + phase: 0 /pseudo
(448 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 78 9e-33
BQ148771 65 7e-11
BG584442 52 4e-07
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 52 4e-07
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 51 1e-06
AI737603 38 0.007
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro... 33 0.22
BQ751066 33 0.29
TC82948 31 1.1
TC87677 29 4.2
AW585117 28 7.2
TC89930 similar to PIR|T47694|T47694 probable serine/threonine-s... 28 7.2
TC88077 similar to GP|15028127|gb|AAK76687.1 unknown protein {Ar... 28 7.2
BF650593 28 7.2
BE249146 similar to GP|14422289|emb cyclin-dependent kinase inhi... 28 9.4
TC81160 similar to GP|14422289|emb|CAC41617. cyclin-dependent ki... 28 9.4
TC82763 28 9.4
TC86504 similar to GP|15028127|gb|AAK76687.1 unknown protein {Ar... 28 9.4
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 77.8 bits (190), Expect(3) = 9e-33
Identities = 36/77 (46%), Positives = 47/77 (60%)
Frame = +3
Query: 300 GDLEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWRDRWCGDVPLC 359
G L +GGR S WW+ I ++R+G+ EG WF +R VGDG FW D W GDVPL
Sbjct: 147 GWLCEGGRQSSMWWKTICKVREGVGEGVGNWFEENIRMVVGDGRDAFFWYDTWAGDVPLR 326
Query: 360 DRFSRLYDLAVNKSITV 376
++ RL DLA++K V
Sbjct: 327 LKYPRLLDLAMDKECKV 377
Score = 61.2 bits (147), Expect(3) = 9e-33
Identities = 28/44 (63%), Positives = 33/44 (74%)
Frame = +1
Query: 253 GLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGG 296
GLGV FN++LLGKWCWRLL+D+E LW +VL ARYG GG
Sbjct: 31 GLGVGA---FNLSLLGKWCWRLLVDKEGLWHRVLKARYGEEGGG 153
Score = 39.7 bits (91), Expect(3) = 9e-33
Identities = 18/35 (51%), Positives = 22/35 (62%)
Frame = +2
Query: 378 NMFLLGVDVGGEALRWRRRLWAWEEELVEECRVVL 412
+M LG V G A W RRL+ WEEE V EC ++L
Sbjct: 383 DMGRLGWTVDGRAWEWTRRLFVWEEECVRECCILL 487
>BQ148771
Length = 680
Score = 64.7 bits (156), Expect = 7e-11
Identities = 44/140 (31%), Positives = 70/140 (49%), Gaps = 2/140 (1%)
Frame = -3
Query: 182 WLAKSVPFFR--ILLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS* 239
W A + R L KSV+ A+P+Y + P I I+ L KF WG+ E R+
Sbjct: 564 WKANHLSLARRVTLAKSVIEAVPLYPMMTTIIPKACIEEIQKLQRKFVWGDTEVSRRYHA 385
Query: 240 IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGGLED 299
+ W+T+S K GLG+ RL N A + K W + +L +V+ +Y ++ LE+
Sbjct: 384 VGWETMSKPKTIYGLGLRRLDVMNKACIMKLGWSIYSGSNSLCTEVMRGKYQRSE-SLEE 208
Query: 300 GDLEDGGRSCSSWWREIVRI 319
LE + SS W+ +V++
Sbjct: 207 IFLEK--PTDSSLWKALVKL 154
>BG584442
Length = 775
Score = 52.4 bits (124), Expect = 4e-07
Identities = 27/92 (29%), Positives = 50/92 (54%), Gaps = 1/92 (1%)
Frame = +1
Query: 192 ILLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGE-GEDKRKIS*IRWDTLSLRKE 250
+++K L ++ Y +S F + + I+ ++N F W GE+++ + + + L + K
Sbjct: 430 VMIKYALQSISSYVMSIFLLLNSQVDEIEKIMNTFSWVHVGENRKGMHWMS*EKLFVHKN 609
Query: 251 YGGLGVTRLREFNVALLGKWCWRLLLDREALW 282
YGG+G T FN+ +LGK LL+R L+
Sbjct: 610 YGGMGFTDFTTFNIPMLGKQV*SFLLNRTTLF 705
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 52.4 bits (124), Expect = 4e-07
Identities = 35/113 (30%), Positives = 56/113 (48%), Gaps = 9/113 (7%)
Frame = -2
Query: 304 DGGRS----CSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWRDRWCGDVPLC 359
DGG + S WW++++ + + G WF + R+VGDG FW+D W VPL
Sbjct: 808 DGGENWPAYASRWWKDLMSLEE---VGRVRWFPRELIRKVGDGRSSFFWKDAWDSSVPLR 638
Query: 360 DRFSRLYDLAVNKSITVRNMFLLGVDVGGEALRWR---RR--LWAWEEELVEE 407
+ F R + + +++ D+ E +RWR RR L+ WE+E + E
Sbjct: 637 ESFPRAFFPYRLLKMGCGDLW----DMNAEGVRWRLYWRRLELFEWEKERLLE 491
Score = 51.6 bits (122), Expect = 6e-07
Identities = 30/70 (42%), Positives = 40/70 (56%), Gaps = 7/70 (10%)
Frame = -3
Query: 252 GGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYG-------VADGGLEDGDLED 304
GGLGV +R NV+LL KW WRLL D+ +LW++VL YG + G + L+D
Sbjct: 951 GGLGVRDIRLVNVSLLAKWWWRLLQDQSSLWKEVLEDIYGPRVSSRTMMVGRIGRLMLQD 772
Query: 305 GGRSCSSWWR 314
GG+ W R
Sbjct: 771 GGKI**VWRR 742
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 50.8 bits (120), Expect = 1e-06
Identities = 66/217 (30%), Positives = 88/217 (40%), Gaps = 3/217 (1%)
Frame = +1
Query: 1 YVVPDVVVKVD*RVRWYRYCLSSCEW*SYR*VSS*TGFTSG*PFISFFIFIGRRFECVDA 60
YV V KVD*RV Y S+CEW S + C D
Sbjct: 379 YVFSGSVEKVD*RVC*YSNNFSACEWESDK--------------------------CSDE 480
Query: 61 SVGRRRNVYGV*CGAHGSSGCVTFTIC**YDSYWE*KLG*CAGTEGWSRFV*SYVGIESK 120
G VY V* SS +T +IC + *KLG ++G + + YV E +
Sbjct: 481 IFGSDSIVY*V*FWGG*SSCGITSSICKRHFVARN*KLGKHPCSKGCTCYFLGYVWFEGE 660
Query: 121 LP*EFSGRG*Y**FVVFRSGFYVGLQSR*NPVSLFGALDWG*SALIIVLGTCC*SY*N*I 180
*E+ G Y F+V GF L+S + + G + + LGT C S+ +*+
Sbjct: 661 FS*EWLGLCEYRSFLVK*GGFCFKLESGQGTIFVLRYAYRGKFSALEFLGTYCESHKS*V 840
Query: 181 VWLAKSVPFFRILLKSVLTALPVY---ALSFFKAPSG 214
WL S F SV ++ SFFKAPSG
Sbjct: 841 DWLE*SFFIFWRPSSSVKVGSDLFVCLCASFFKAPSG 951
>AI737603
Length = 531
Score = 38.1 bits (87), Expect = 0.007
Identities = 18/38 (47%), Positives = 22/38 (57%)
Frame = -2
Query: 306 GRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGA 343
GR SS WR + IR G+ G GWF +RR VG G+
Sbjct: 125 GRRDSSVWRMLSAIRSGVGSGEGGWFEENIRRMVGGGS 12
>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 621
Score = 33.1 bits (74), Expect = 0.22
Identities = 15/34 (44%), Positives = 24/34 (70%)
Frame = +3
Query: 242 WDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLL 275
++ L+ K GGLG+ +EFN+++LGK W+LL
Sbjct: 303 FNNLARHKSVGGLGLQN-QEFNISMLGKQRWKLL 401
>BQ751066
Length = 750
Score = 32.7 bits (73), Expect = 0.29
Identities = 21/68 (30%), Positives = 32/68 (46%)
Frame = -2
Query: 286 LVARYGVADGGLEDGDLEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKI 345
+V+R G +GG+E + G + WR R R GI G G ++R G +
Sbjct: 314 IVSREGEVNGGVEVLRMSRGAKMSVKEWRPFARARLGIRTGVAGQRRDRLKR---TGLER 144
Query: 346 DFWRDRWC 353
+R+RWC
Sbjct: 143 LLYRERWC 120
>TC82948
Length = 705
Score = 30.8 bits (68), Expect = 1.1
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = +3
Query: 236 KIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDRE 279
K+ + W+ + + G LG+ L + N AL K CW +++ +E
Sbjct: 255 KVVKVSWEKVCRPIKEGSLGIRSLSKLNEALNLKLCWDMMISKE 386
>TC87677
Length = 682
Score = 28.9 bits (63), Expect = 4.2
Identities = 11/23 (47%), Positives = 15/23 (64%)
Frame = +3
Query: 269 KWCWRLLLDREALWRKVLVARYG 291
K CW ++L+ +W VL ARYG
Sbjct: 255 K*CWMIILESLIMWWDVLYARYG 323
>AW585117
Length = 514
Score = 28.1 bits (61), Expect = 7.2
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = -3
Query: 267 LGKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSSWWREIVRIRDGICEG 326
LG CW + D + + YG+ G +ED +DG +++RE V +R G
Sbjct: 392 LGLDCWDAVNDGDVFDGEFYFGTYGLFAGCIEDVS-DDGNGDNLNFYREFV*LRRSYVAG 216
Query: 327 -GEG 329
GEG
Sbjct: 215 YGEG 204
>TC89930 similar to PIR|T47694|T47694 probable serine/threonine-specific
protein kinase - Arabidopsis thaliana, partial (53%)
Length = 1449
Score = 28.1 bits (61), Expect = 7.2
Identities = 37/125 (29%), Positives = 53/125 (41%), Gaps = 5/125 (4%)
Frame = +2
Query: 256 VTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSSWWRE 315
+ RLR N+ +L WC R R L +LV Y +A+G L+ ED S W +
Sbjct: 1022 IGRLRHRNLVMLLGWCRR----RGDL---LLVYDY-MANGSLDKYLFEDSEYVLS--WEQ 1171
Query: 316 IVRIRDGICEG----GEGWFGACVRRRV-GDGAKIDFWRDRWCGDVPLCDRFSRLYDLAV 370
+I G+ G EG+ + R V +DF + GD L +RLY+
Sbjct: 1172 RFKIIKGVASGLLYLHEGYEQVVIHRDVKASNVLLDFELNGRLGDFGL----ARLYEHGA 1339
Query: 371 NKSIT 375
N T
Sbjct: 1340 NPGTT 1354
>TC88077 similar to GP|15028127|gb|AAK76687.1 unknown protein {Arabidopsis
thaliana}, partial (27%)
Length = 1130
Score = 28.1 bits (61), Expect = 7.2
Identities = 16/55 (29%), Positives = 19/55 (34%), Gaps = 3/55 (5%)
Frame = +3
Query: 302 LEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWR---DRWC 353
L +G R SSW C GW + GA + FWR WC
Sbjct: 843 LTEGWRISSSWCTWAFSTNSSTCANALGWLDV---KSYSCGASLSFWRRSYKSWC 998
>BF650593
Length = 486
Score = 28.1 bits (61), Expect = 7.2
Identities = 15/42 (35%), Positives = 20/42 (46%)
Frame = +1
Query: 281 LWRKVLVARYGVADGGLEDGDLEDGGRSCSSWWREIVRIRDG 322
LW + LV +YG L G + R S WW++I I G
Sbjct: 88 LWFRALVNKYG-----LNRGSITIENRGVSLWWKDICYIDFG 198
>BE249146 similar to GP|14422289|emb cyclin-dependent kinase inhibitor 3
{Arabidopsis thaliana}, partial (12%)
Length = 638
Score = 27.7 bits (60), Expect = 9.4
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1
Query: 248 RKEYGGLGVTRLREFNVALLGKWC 271
RKE G G +RLRE +V LG +C
Sbjct: 277 RKESGSPGKSRLRECSVEKLGNFC 348
>TC81160 similar to GP|14422289|emb|CAC41617. cyclin-dependent kinase
inhibitor 3 {Arabidopsis thaliana}, partial (38%)
Length = 805
Score = 27.7 bits (60), Expect = 9.4
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +3
Query: 248 RKEYGGLGVTRLREFNVALLGKWC 271
RKE G G +RLRE +V LG +C
Sbjct: 270 RKESGSPGKSRLRECSVEKLGNFC 341
>TC82763
Length = 1376
Score = 27.7 bits (60), Expect = 9.4
Identities = 22/72 (30%), Positives = 31/72 (42%)
Frame = +1
Query: 252 GGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSS 311
GG+ + LR FN+A+ W L + + VL +YG LE S S
Sbjct: 271 GGIRIRNLRFFNIAVR**-SWTLKTETNSFHFMVLFHKYG--------SWLEGVNCSSSL 423
Query: 312 WWREIVRIRDGI 323
WWR + I G+
Sbjct: 424 WWRYLSSIDTGM 459
>TC86504 similar to GP|15028127|gb|AAK76687.1 unknown protein {Arabidopsis
thaliana}, partial (30%)
Length = 1121
Score = 27.7 bits (60), Expect = 9.4
Identities = 16/55 (29%), Positives = 19/55 (34%), Gaps = 3/55 (5%)
Frame = +2
Query: 302 LEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWR---DRWC 353
L +G R SSW C GW + GA + FWR WC
Sbjct: 119 LTEGWRISSSWCTWAFSTNSSTCANALGWLDV---KSYSCGASLSFWRRSYRSWC 274
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.353 0.160 0.608
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,009,632
Number of Sequences: 36976
Number of extensions: 255275
Number of successful extensions: 3484
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1972
Number of HSP's successfully gapped in prelim test: 157
Number of HSP's that attempted gapping in prelim test: 1424
Number of HSP's gapped (non-prelim): 2261
length of query: 448
length of database: 9,014,727
effective HSP length: 99
effective length of query: 349
effective length of database: 5,354,103
effective search space: 1868581947
effective search space used: 1868581947
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC138580.5