BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138464.6 + phase: 0
(250 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83899 similar to GP|1001955|gb|AAA76862.1| unknown protein {Ly... 210 4e-55
BE940998 similar to PIR|A86414|A86 hypothetical protein AAF88122... 34 0.061
TC77664 similar to PIR|A86414|A86414 hypothetical protein AAF881... 31 0.39
TC87013 weakly similar to PIR|T04962|T04962 catechol O-methyltra... 28 2.5
TC85012 weakly similar to PIR|T05754|T05754 S-receptor kinase (E... 28 3.3
TC89709 weakly similar to GP|3868810|dbj|BAA34233.1 SRK23Bol {Br... 27 5.7
BG447960 weakly similar to GP|1272349|gb|A secreted glycoprotein... 27 7.4
TC87939 similar to PIR|T48615|T48615 hypothetical protein F18O22... 27 7.4
TC77491 similar to GP|13937149|gb|AAK50068.1 At1g79380/T8K14_20 ... 27 9.7
>TC83899 similar to GP|1001955|gb|AAA76862.1| unknown protein {Lycopersicon
chilense}, partial (43%)
Length = 670
Score = 210 bits (535), Expect = 4e-55
Identities = 106/106 (100%), Positives = 106/106 (100%)
Frame = +1
Query: 115 MYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRFL 174
MYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRFL
Sbjct: 1 MYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRFL 180
Query: 175 EDPEQYKTCEMIEHIDSSIQRIMALSKKLTATDEQISCDQLYLSKV 220
EDPEQYKTCEMIEHIDSSIQRIMALSKKLTATDEQISCDQLYLSKV
Sbjct: 181 EDPEQYKTCEMIEHIDSSIQRIMALSKKLTATDEQISCDQLYLSKV 318
>BE940998 similar to PIR|A86414|A86 hypothetical protein AAF88122.1
[imported] - Arabidopsis thaliana, partial (28%)
Length = 475
Score = 33.9 bits (76), Expect = 0.061
Identities = 25/107 (23%), Positives = 50/107 (46%), Gaps = 8/107 (7%)
Frame = +1
Query: 107 LAKQVVSSMY----KRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYA 162
+ +++SS+Y ++N+ RL + + + + IA + L E + QMI D +
Sbjct: 43 IVHRLISSLYDTLLEQNLCRLIEPFSRVEIAHIAELIEL-PIDHVERKMSQMILDKKFAG 219
Query: 163 TINQKDGMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
T++Q G + +DP+ T E I ++ + + S K+ A
Sbjct: 220 TLDQGAGCLVIFDDPKTDAIYPATLETISNVGKVVDSLYVRSAKIMA 360
>TC77664 similar to PIR|A86414|A86414 hypothetical protein AAF88122.1
[imported] - Arabidopsis thaliana, partial (95%)
Length = 1597
Score = 31.2 bits (69), Expect = 0.39
Identities = 22/98 (22%), Positives = 44/98 (44%), Gaps = 4/98 (4%)
Frame = +3
Query: 114 SMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKDGMVRF 173
++ ++N+ RL + + + + IA + L E + QMI D + T++Q G +
Sbjct: 1083 TLLEQNLCRLIEPFSRVEIAHIAELIEL-PIDHVERKMSQMILDKKFAGTLDQGAGCLVI 1259
Query: 174 LEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTATD 207
+DP+ T E I ++ + + S K+ D
Sbjct: 1260 FDDPKTDAIYPATLETISNVGKVVDSLFVRSAKIMT*D 1373
>TC87013 weakly similar to PIR|T04962|T04962 catechol O-methyltransferase
homolog T12J5.20 - Arabidopsis thaliana, partial (59%)
Length = 1607
Score = 28.5 bits (62), Expect = 2.5
Identities = 24/88 (27%), Positives = 39/88 (44%), Gaps = 6/88 (6%)
Frame = +3
Query: 31 VIEAYKKYILVSLIRHGQFSTSLPKYASPLAQRNLKLFCQNPCIELAQTYN------NGK 84
VI+ KY V + G TS+PK + + + L + CI++ + NGK
Sbjct: 879 VIDVAPKYDGVEHVA-GDMFTSVPKADAAIIKWVLHDWGDEECIQILKNCREAIPKENGK 1055
Query: 85 VAEVEAFVNANAGRFEADNNLGLAKQVV 112
V VEA + G+ ++GL +V
Sbjct: 1056VIIVEAVIEEGEGKHNKYKDVGLMLDMV 1139
>TC85012 weakly similar to PIR|T05754|T05754 S-receptor kinase (EC 2.7.1.-)
M4I22.110 precursor - Arabidopsis thaliana, partial
(17%)
Length = 645
Score = 28.1 bits (61), Expect = 3.3
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -3
Query: 195 RIMALSKKLTATDEQISCDQLYLSKVSVQSFSFGYLNLYIEGLNMFS 241
++ A+ K L + SC Q +KVS S YLNL+++ N FS
Sbjct: 163 KMPAVCKCLVLGLDVFSCRQCCQTKVSSHSHHL*YLNLHMQCKNSFS 23
>TC89709 weakly similar to GP|3868810|dbj|BAA34233.1 SRK23Bol {Brassica
oleracea}, partial (9%)
Length = 975
Score = 27.3 bits (59), Expect = 5.7
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = -2
Query: 201 KKLTATDEQISCDQLYLSKVSVQSFSFGYLNLYIEGLNMFS 241
+ L T+ SC + SK + FGY +LY N FS
Sbjct: 590 RDLVPTERMFSCQSDHQSKANNHFLHFGYPHLYKPYTNSFS 468
>BG447960 weakly similar to GP|1272349|gb|A secreted glycoprotein 3 {Ipomoea
trifida}, partial (17%)
Length = 664
Score = 26.9 bits (58), Expect = 7.4
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = -1
Query: 201 KKLTATDEQISCDQLYLSKVSVQSFSFGYLNLYIEGLNMFS 241
+ L T+ SC + SK + FGY +LY N FS
Sbjct: 265 RDLVPTERMFSCQPDHQSKANNHFLHFGYPHLYKPYTNSFS 143
>TC87939 similar to PIR|T48615|T48615 hypothetical protein F18O22.210 -
Arabidopsis thaliana, partial (69%)
Length = 1612
Score = 26.9 bits (58), Expect = 7.4
Identities = 20/88 (22%), Positives = 38/88 (42%)
Frame = +1
Query: 99 FEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDG 158
F A++ + V S Y+ + +L T I + + E + HVL +I DG
Sbjct: 610 FNAEDRFCHGFEEVLSRYRELVPQLRLAGPTSFAPIIEMAITIVEQSEGQYHVLLIIADG 789
Query: 159 EIYATINQKDGMVRFLEDPEQYKTCEMI 186
++ +++ G + ++ KT E I
Sbjct: 790 QVTRSVDVDHGQL----SAQEKKTVEAI 861
>TC77491 similar to GP|13937149|gb|AAK50068.1 At1g79380/T8K14_20
{Arabidopsis thaliana}, partial (81%)
Length = 1537
Score = 26.6 bits (57), Expect = 9.7
Identities = 20/93 (21%), Positives = 41/93 (43%)
Frame = +2
Query: 99 FEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDG 158
F AD++ + V + Y++ + L+ + T I + + + HVL ++ DG
Sbjct: 497 FHADDSSCHGFEEVLACYQKIVPNLSLSGPTSYAPVIEAAMDIVEKTHGQFHVLVIVADG 676
Query: 159 EIYATINQKDGMVRFLEDPEQYKTCEMIEHIDS 191
++ N + G + P++ KT + I S
Sbjct: 677 QVTRYGNNEGGKL----SPQEEKTIKAIADASS 763
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.133 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,519,838
Number of Sequences: 36976
Number of extensions: 74077
Number of successful extensions: 376
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 376
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 376
length of query: 250
length of database: 9,014,727
effective HSP length: 94
effective length of query: 156
effective length of database: 5,538,983
effective search space: 864081348
effective search space used: 864081348
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC138464.6