BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138464.5 + phase: 0
(235 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79486 weakly similar to GP|16930463|gb|AAL31917.1 AT5g14250/F1... 342 6e-95
TC83341 weakly similar to GP|8978076|dbj|BAA98104.1 gene_id:K14A... 28 0.78
TC77684 similar to GP|21554321|gb|AAM63426.1 NTGP5 {Arabidopsis ... 28 2.3
TC77023 calmodulin 2 [Medicago truncatula] 28 2.3
TC89753 similar to GP|14334556|gb|AAK59686.1 unknown protein {Ar... 28 3.0
TC85794 homologue to SP|Q9SM60|PGMU_PEA Phosphoglucomutase cyto... 28 4.0
TC86501 homologue to GP|6984224|gb|AAF34800.1| 60S ribosomal pro... 28 4.0
BQ157979 similar to PIR|JQ0253|F2RZ0 photosystem II phosphoprote... 27 5.2
TC83003 27 5.2
TC80050 similar to GP|5081557|gb|AAD39440.1| PHAP2B protein {Pet... 27 5.2
AW690832 similar to GP|6056196|gb| unknown protein {Arabidopsis ... 27 6.8
TC83911 homologue to GP|21449351|gb|AAM54083.1 Na+/H+ antiporter... 27 6.8
CB892510 similar to GP|21554325|gb| metal ion transporter {Arabi... 27 6.8
BG457950 similar to GP|4996286|dbj lipoyltransferase {Arabidopsi... 27 6.8
TC83798 weakly similar to GP|10177750|dbj|BAB11063. cytochrome P... 27 8.9
TC82856 homologue to PIR|T49914|T49914 callose synthase catalyti... 27 8.9
AL375169 GP|4959710|gb| Skp1 {Medicago sativa}, partial (18%) 27 8.9
>TC79486 weakly similar to GP|16930463|gb|AAL31917.1 AT5g14250/F18O22_40
{Arabidopsis thaliana}, partial (25%)
Length = 628
Score = 342 bits (877), Expect(2) = 6e-95
Identities = 176/177 (99%), Positives = 176/177 (99%)
Frame = +2
Query: 1 MDPLEPLIAQIQGLSSSSADIARLHTILKHSDETLRSDSSRLYPILQLIDPSIHSLGFLY 60
MDPLEPLIAQIQGLSSSSADIARLHTILKHSDETLRSDSSRLYPILQLIDPSIHSLGFLY
Sbjct: 62 MDPLEPLIAQIQGLSSSSADIARLHTILKHSDETLRSDSSRLYPILQLIDPSIHSLGFLY 241
Query: 61 LLDAFSAASVSNQQQAEEAVPIIARFINACSVEQIRLAPEKFVSVCKRLKDLVTLLEAPI 120
LLDAFSAASVSNQQQAEEAVPIIARFINACSVEQIRLAPEKFVSVCKRLKDLVTLLEAPI
Sbjct: 242 LLDAFSAASVSNQQQAEEAVPIIARFINACSVEQIRLAPEKFVSVCKRLKDLVTLLEAPI 421
Query: 121 RGVAPMFTALRKLQVSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDVFEVDHPRDL 177
RGVAPMFTALRKLQVSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDVFEV HPRDL
Sbjct: 422 RGVAPMFTALRKLQVSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDVFEVXHPRDL 592
Score = 22.7 bits (47), Expect(2) = 6e-95
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +3
Query: 178 YLYCYYG 184
YLYCYYG
Sbjct: 594 YLYCYYG 614
>TC83341 weakly similar to GP|8978076|dbj|BAA98104.1 gene_id:K14A3.4~unknown
protein {Arabidopsis thaliana}, partial (80%)
Length = 1253
Score = 28.1 bits (61), Expect(2) = 0.78
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = -3
Query: 199 RCRICSYISVLHFSYITYCNFFNILDLFFTSF 230
RC I +YI + H + FFNI+ +FF F
Sbjct: 711 RCAIVAYIRLRHVYLLHSLVFFNIIVIFFFLF 616
Score = 20.4 bits (41), Expect(2) = 0.78
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = -2
Query: 174 PRDLYLYCYYGCVAFFFSFELEKRDRCRI 202
P L+L+ C SFEL++ CR+
Sbjct: 778 PALLWLHQMCHC*LHHVSFELDQMCHCRL 692
>TC77684 similar to GP|21554321|gb|AAM63426.1 NTGP5 {Arabidopsis thaliana},
partial (56%)
Length = 1151
Score = 28.5 bits (62), Expect = 2.3
Identities = 15/43 (34%), Positives = 23/43 (52%), Gaps = 2/43 (4%)
Frame = +1
Query: 177 LYLYCYYGCVAFFFSFELEKRDRCRI--CSYISVLHFSYITYC 217
LYL C C+ F + ++ CR+ Y++VL F+Y YC
Sbjct: 1021 LYLMC---CILTFLLYLCVMQNVCRLKCVEYLNVLCFAYCLYC 1140
>TC77023 calmodulin 2 [Medicago truncatula]
Length = 999
Score = 28.5 bits (62), Expect = 2.3
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +2
Query: 180 YCYYGCVAFF-FSFELEKRDRCRICSYISVLHFS 212
YCYY + F+ F+ L +C Y VLHFS
Sbjct: 698 YCYYSLLVFYLFALSLCLSSLN*LCPYAFVLHFS 799
>TC89753 similar to GP|14334556|gb|AAK59686.1 unknown protein {Arabidopsis
thaliana}, partial (6%)
Length = 1062
Score = 28.1 bits (61), Expect = 3.0
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +1
Query: 210 HFSYITYCNFFNILDLFFT 228
HF Y YC NIL FFT
Sbjct: 127 HFDYWVYCFVINILSFFFT 183
>TC85794 homologue to SP|Q9SM60|PGMU_PEA Phosphoglucomutase cytoplasmic (EC
5.4.2.2) (Glucose phosphomutase) (PGM). [Garden pea],
complete
Length = 2175
Score = 27.7 bits (60), Expect = 4.0
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = -3
Query: 61 LLDAFSAASVSNQQQAEEAVPIIARFINACSVEQIRLAP 99
+ ++F+AAS + E++PI F + CS+ + +AP
Sbjct: 1900 IFESFNAASTRGASASCESLPIFDGFFSYCSMYSLIVAP 1784
>TC86501 homologue to GP|6984224|gb|AAF34800.1| 60S ribosomal protein L35
{Euphorbia esula}, partial (96%)
Length = 936
Score = 27.7 bits (60), Expect = 4.0
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 179 LYCYYGCVAFFFSFELEKRDRCRICSYISVLHFSY 213
L C G VA+ F F +CSYIS H+S+
Sbjct: 497 LICLSGSVAYAFQF---------LCSYISDFHYSF 574
>BQ157979 similar to PIR|JQ0253|F2RZ0 photosystem II phosphoprotein psbH -
rice chloroplast, partial (41%)
Length = 920
Score = 27.3 bits (59), Expect = 5.2
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -2
Query: 208 VLHFSYITYCNFFNILDLFFTSFS 231
+LH S+ YC F L FF SFS
Sbjct: 754 ILHSSHTLYCLFCCCLSFFFCSFS 683
Score = 27.3 bits (59), Expect = 5.2
Identities = 19/52 (36%), Positives = 23/52 (43%), Gaps = 6/52 (11%)
Frame = -1
Query: 175 RDLYLYC--YYGCVAFFFSFELEKRDRCRICSYIS----VLHFSYITYCNFF 220
R + C YY CVA F F L R S +S L F Y +Y NF+
Sbjct: 587 RSFFFRCVVYYSCVAVFLLFILSYYTSLRYVSCLSSAV*FLCFVYGSYDNFY 432
>TC83003
Length = 500
Score = 27.3 bits (59), Expect = 5.2
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -2
Query: 201 RICSYISVLHFSYITYCNFFNI 222
+I S+IS LHF YC FNI
Sbjct: 289 KIISHISKLHFCLTNYCPNFNI 224
>TC80050 similar to GP|5081557|gb|AAD39440.1| PHAP2B protein {Petunia x
hybrida}, partial (10%)
Length = 931
Score = 27.3 bits (59), Expect = 5.2
Identities = 12/20 (60%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
Frame = +1
Query: 203 CSYISVLHFSYI-TYCNFFN 221
CS I +LHFSY+ T CN N
Sbjct: 574 CSIIRILHFSYLSTKCNLSN 633
>AW690832 similar to GP|6056196|gb| unknown protein {Arabidopsis thaliana},
partial (28%)
Length = 389
Score = 26.9 bits (58), Expect = 6.8
Identities = 21/80 (26%), Positives = 36/80 (44%), Gaps = 1/80 (1%)
Frame = +3
Query: 136 SAEHLTPLHAEFLMLC-LLAKCYKTGLSILDDDVFEVDHPRDLYLYCYYGCVAFFFSFEL 194
S + +TP + EF+ +C L+A Y + + ++ + YL YG F +
Sbjct: 45 SGKMVTPNNKEFMFICFLVALWYSSNIGVI---------LLNKYLISNYG-FKFPIFLTM 194
Query: 195 EKRDRCRICSYISVLHFSYI 214
C I SYIS++ F +
Sbjct: 195 CHMTACAIFSYISIVFFKIV 254
>TC83911 homologue to GP|21449351|gb|AAM54083.1 Na+/H+ antiporter
{Actinosynnema pretiosum subsp. auranticum}, partial
(2%)
Length = 806
Score = 26.9 bits (58), Expect = 6.8
Identities = 19/68 (27%), Positives = 29/68 (41%)
Frame = -2
Query: 119 PIRGVAPMFTALRKLQVSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDVFEVDHPRDLY 178
PIR + + L +++ H +HA F CL + I DD + EV+
Sbjct: 484 PIRLM*RIVHGLVNSNINSLHFVQIHASFSFHCLWMEVTNA**RICDDWLLEVN------ 323
Query: 179 LYCYYGCV 186
Y+GCV
Sbjct: 322 ---YFGCV 308
>CB892510 similar to GP|21554325|gb| metal ion transporter {Arabidopsis
thaliana}, partial (7%)
Length = 687
Score = 26.9 bits (58), Expect = 6.8
Identities = 11/32 (34%), Positives = 16/32 (49%)
Frame = +2
Query: 188 FFFSFELEKRDRCRICSYISVLHFSYITYCNF 219
FFF F + DR +CSY H + +C +
Sbjct: 512 FFFMFSINWHDRKCLCSYGGFPHKCFCYFCKW 607
>BG457950 similar to GP|4996286|dbj lipoyltransferase {Arabidopsis thaliana},
partial (77%)
Length = 686
Score = 26.9 bits (58), Expect = 6.8
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = +1
Query: 196 KRDRCRICSYISVLHFSYI 214
K+DRC CS I HFS+I
Sbjct: 565 KKDRCNWCSDIKCNHFSWI 621
>TC83798 weakly similar to GP|10177750|dbj|BAB11063. cytochrome P450
{Arabidopsis thaliana}, partial (25%)
Length = 762
Score = 26.6 bits (57), Expect = 8.9
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -2
Query: 212 SYITYCNFFNILDLFFTSFSIKC 234
S I+ NFFNIL LF S + C
Sbjct: 107 SLISALNFFNILGLFIISAMVHC 39
>TC82856 homologue to PIR|T49914|T49914 callose synthase catalytic
subunit-like protein - Arabidopsis thaliana, partial
(12%)
Length = 764
Score = 26.6 bits (57), Expect = 8.9
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = +2
Query: 160 GLSILDDDVFEVDHPRDLY--LYCYYGCVAFFFS 191
G L DV+ + H D + L CY+ V F+FS
Sbjct: 272 GEQTLSRDVYRLGHRFDFFRMLSCYFTTVGFYFS 373
>AL375169 GP|4959710|gb| Skp1 {Medicago sativa}, partial (18%)
Length = 466
Score = 26.6 bits (57), Expect = 8.9
Identities = 11/54 (20%), Positives = 24/54 (44%)
Frame = +3
Query: 181 CYYGCVAFFFSFELEKRDRCRICSYISVLHFSYITYCNFFNILDLFFTSFSIKC 234
C F+S + D+C IC + L ++ ++ ++ ++ F +KC
Sbjct: 246 CIVDLCCLFYSSKFRNVDQCYIC*VLVYLLDYFVLSVDYVSLDSVYLYCFELKC 407
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.329 0.142 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,314,440
Number of Sequences: 36976
Number of extensions: 135274
Number of successful extensions: 1210
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 1199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1210
length of query: 235
length of database: 9,014,727
effective HSP length: 93
effective length of query: 142
effective length of database: 5,575,959
effective search space: 791786178
effective search space used: 791786178
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC138464.5