BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138130.21 - phase: 0 /pseudo
(247 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80712 similar to GP|17979410|gb|AAL49847.1 unknown protein {Ar... 509 e-145
TC87418 similar to PIR|T51264|T51264 calcium-dependent protein k... 30 0.66
TC81629 similar to GP|15081654|gb|AAK82482.1 AT4g26750/F10M23_90... 30 0.86
BG587258 homologue to PIR|T50532|T5053 ribosomal protein S27 - A... 29 1.5
TC85234 homologue to PIR|T50532|T50532 ribosomal protein S27 - A... 29 1.5
BQ144271 weakly similar to GP|21322711|em pherophorin-dz1 protei... 29 1.9
TC80054 SP|Q28009|FUS_BOVIN RNA-binding protein FUS (Pigpen prot... 29 1.9
TC78352 homologue to PIR|T14086|T14086 hypothetical protein F9N1... 28 2.5
TC87153 similar to GP|20804774|dbj|BAB92459. hypothetical protei... 28 2.5
TC89567 similar to GP|12328581|dbj|BAB21240. Putative protein ki... 28 2.5
TC86141 similar to GP|20260580|gb|AAM13188.1 unknown protein {Ar... 28 3.3
TC82446 similar to GP|19071882|dbj|BAB85678. small integral memb... 28 3.3
BQ137054 homologue to PIR|T46107|T461 hypothetical protein T25B1... 28 3.3
TC83704 similar to PIR|T05005|T05005 hypothetical protein T19P19... 28 4.3
BQ751277 similar to PIR|T37665|T37 probable t-complex protein 1 ... 28 4.3
BE325816 weakly similar to GP|15028133|gb| unknown protein {Arab... 27 5.6
TC90113 similar to PIR|C86301|C86301 arginine/serine-rich protei... 27 5.6
TC88498 similar to GP|21304712|gb|AAM45475.1 ethylene-responsive... 27 5.6
TC76664 weakly similar to PIR|T08541|T08541 hypothetical protein... 27 5.6
AW697008 similar to GP|2981301|gb|A 25-hydroxyvitamin D3 24-hydr... 27 7.3
>TC80712 similar to GP|17979410|gb|AAL49847.1 unknown protein {Arabidopsis
thaliana}, partial (72%)
Length = 1424
Score = 509 bits (1310), Expect = e-145
Identities = 247/247 (100%), Positives = 247/247 (100%)
Frame = +3
Query: 1 MSTTAQRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPPPPFF 60
MSTTAQRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPPPPFF
Sbjct: 144 MSTTAQRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENFGNSDLPPPPFF 323
Query: 61 TLEDRSDFSPESGILDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEASTSYALRGVQQN 120
TLEDRSDFSPESGILDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEASTSYALRGVQQN
Sbjct: 324 TLEDRSDFSPESGILDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEASTSYALRGVQQN 503
Query: 121 QQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNSLPAG 180
QQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNSLPAG
Sbjct: 504 QQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQRNSLPAG 683
Query: 181 NLNEEEWVTVYGFSPNDTNLVLREFEKCGEILKHVPGPRDANWMHILYQNRSAAQNALNK 240
NLNEEEWVTVYGFSPNDTNLVLREFEKCGEILKHVPGPRDANWMHILYQNRSAAQNALNK
Sbjct: 684 NLNEEEWVTVYGFSPNDTNLVLREFEKCGEILKHVPGPRDANWMHILYQNRSAAQNALNK 863
Query: 241 NGIQING 247
NGIQING
Sbjct: 864 NGIQING 884
>TC87418 similar to PIR|T51264|T51264 calcium-dependent protein kinase-like
- Arabidopsis thaliana, partial (30%)
Length = 1206
Score = 30.4 bits (67), Expect = 0.66
Identities = 31/105 (29%), Positives = 44/105 (41%), Gaps = 14/105 (13%)
Frame = +1
Query: 46 RENFGNSD---LPPPPFFTLEDRSD---------FSPESGILDYQMSP--ETKSDHKTPI 91
REN N + LPPPP + + FSP ++ SP T S + TP+
Sbjct: 502 RENSTNEEILQLPPPPSSSHHHHAPTKSSSKFPFFSPSPLPSFFKNSPANSTVSSNSTPL 681
Query: 92 QTPNREFSTPAKGKSEASTSYALRGVQQNQQGSLSLNWWSSPAKS 136
+TP R F P S A +R + + GS+ N + P S
Sbjct: 682 RTPLRIFKRPFPPPSPAK---HIRALLARRHGSVKPNEATIPEGS 807
>TC81629 similar to GP|15081654|gb|AAK82482.1 AT4g26750/F10M23_90
{Arabidopsis thaliana}, partial (19%)
Length = 824
Score = 30.0 bits (66), Expect = 0.86
Identities = 21/79 (26%), Positives = 31/79 (38%)
Frame = +2
Query: 51 NSDLPPPPFFTLEDRSDFSPESGILDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEAST 110
+ D PPPP D+ P DYQ P ++ H+ P PA+ S S
Sbjct: 80 SQDYPPPP------SQDYHPPPPSQDYQPPPPSQDYHQPP----------PARSDSSYSE 211
Query: 111 SYALRGVQQNQQGSLSLNW 129
Y + +Q +L N+
Sbjct: 212 PYNHQQYSPDQSQNLGPNY 268
>BG587258 homologue to PIR|T50532|T5053 ribosomal protein S27 - Arabidopsis
thaliana, partial (98%)
Length = 663
Score = 29.3 bits (64), Expect = 1.5
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +2
Query: 115 RGVQQNQQGSLSLNWWSSPAKSGGEQEEKG 144
RG++Q G + NWW S A G +E+G
Sbjct: 194 RGLRQLSDGFVPANWWQSKANRGVLLQEEG 283
>TC85234 homologue to PIR|T50532|T50532 ribosomal protein S27 - Arabidopsis
thaliana, complete
Length = 813
Score = 29.3 bits (64), Expect = 1.5
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +3
Query: 115 RGVQQNQQGSLSLNWWSSPAKSGGEQEEKG 144
RG++Q G + NWW S A G +E+G
Sbjct: 492 RGLRQLSDGFVPANWWQSKANRGVLLQEEG 581
>BQ144271 weakly similar to GP|21322711|em pherophorin-dz1 protein {Volvox
carteri f. nagariensis}, partial (5%)
Length = 919
Score = 28.9 bits (63), Expect = 1.9
Identities = 28/101 (27%), Positives = 39/101 (37%), Gaps = 7/101 (6%)
Frame = +2
Query: 80 SPETKSDHKTPIQTPNREF--STPAKGKSEASTSYALRGVQQNQQGSLSLNWWS-----S 132
+P T+S P+ PN + +T + + S A RG QQ Q N
Sbjct: 221 TPTTQSKPHPPVPLPNTKVKANT*IQPTPHRTESRAQRGNQQYYQAVRPGNRKGRRK*RE 400
Query: 133 PAKSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPEVQ 173
K G + + KG G P +G P A P +PE Q
Sbjct: 401 RGKGGKKGK*KGEGGPPKGTAMPAAREARWPSKGGKKPEKQ 523
>TC80054 SP|Q28009|FUS_BOVIN RNA-binding protein FUS (Pigpen protein).
[Bovine] {Bos taurus}, partial (4%)
Length = 988
Score = 28.9 bits (63), Expect = 1.9
Identities = 42/181 (23%), Positives = 67/181 (36%), Gaps = 16/181 (8%)
Frame = +1
Query: 6 QRTPRSG-RQSLFFQDLASPVNARRGK----FSSPGQSGASS----SLWRENFGNSDLPP 56
Q+ P+SG Q + +A+ G+ + S GQ A+ ++ +G+S P
Sbjct: 190 QQPPQSGYAQPTSYDQQQGYGSAQEGQTTTNYGSQGQGDATQVPPVQPSQQGYGSSQQPS 369
Query: 57 PPFFTLEDRSDFSPESGI-----LDYQMSPETKSDHKTPIQTPNREFSTPAKGKSEASTS 111
P + P G+ Y P+T+S + P QT + PA G+S+
Sbjct: 370 PNAAKYPPQGAAQPSYGVPPTSQTAYGSQPQTQSGYGPP-QTQKPSGTPPAYGQSQ---- 534
Query: 112 YALRGVQQNQQGSLSLNWWSSPAKSGGEQEEKGRGS--PVEGVVQPGALITLPPPPEVAR 169
SP +GG + S P G QPG + PPP ++
Sbjct: 535 --------------------SPNAAGGYGQPGYPPSQPPPSGYSQPGYPPSQPPPSGYSQ 654
Query: 170 P 170
P
Sbjct: 655 P 657
>TC78352 homologue to PIR|T14086|T14086 hypothetical protein F9N11.110 -
Arabidopsis thaliana, partial (65%)
Length = 1179
Score = 28.5 bits (62), Expect = 2.5
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
Frame = +3
Query: 157 ALITLPPPPEVARPEVQRNSL-PAGN-LNEEEWVTV 190
+L LPPPP RP++ + PA N L E W TV
Sbjct: 354 SLPPLPPPPSSTRPDISTSGFGPAPNTLTEPVWDTV 461
>TC87153 similar to GP|20804774|dbj|BAB92459. hypothetical protein~similar
to Arabidopsis thaliana chromosome 1 F10C21_14, partial
(50%)
Length = 920
Score = 28.5 bits (62), Expect = 2.5
Identities = 15/54 (27%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = -3
Query: 30 GKFSSPGQSGAS--SSLWRENFGNSDLPPPPFFTLEDRSDFSPESGILDYQMSP 81
GK ++ G++ + + +W+E LPPPP F +E ++P+ L + P
Sbjct: 681 GKVAAEGKADPTPPNPVWKEPITLMFLPPPPCFGVEGLDLWTPKEARLQLALLP 520
>TC89567 similar to GP|12328581|dbj|BAB21240. Putative protein kinase {Oryza
sativa (japonica cultivar-group)}, partial (21%)
Length = 1003
Score = 28.5 bits (62), Expect = 2.5
Identities = 21/78 (26%), Positives = 39/78 (49%)
Frame = -3
Query: 106 SEASTSYALRGVQQNQQGSLSLNWWSSPAKSGGEQEEKGRGSPVEGVVQPGALITLPPPP 165
S ++ S+A R V ++ SL +N W P + +KG G+ V+G L T+ P
Sbjct: 971 SPSTFSFANRFVASSR--SLKVNQWPQPK*ENSGRPDKGEGA-VKGYEAEECLYTVTVPE 801
Query: 166 EVARPEVQRNSLPAGNLN 183
++ P+ + + P +L+
Sbjct: 800 LLSSPDCECDPPPPISLH 747
>TC86141 similar to GP|20260580|gb|AAM13188.1 unknown protein {Arabidopsis
thaliana}, partial (82%)
Length = 1876
Score = 28.1 bits (61), Expect = 3.3
Identities = 13/32 (40%), Positives = 17/32 (52%)
Frame = -2
Query: 115 RGVQQNQQGSLSLNWWSSPAKSGGEQEEKGRG 146
R VQ+ S + WW +SGGE +GRG
Sbjct: 357 REVQRGWSWS*FVRWWWGVMRSGGEGGREGRG 262
>TC82446 similar to GP|19071882|dbj|BAB85678. small integral membrane
protein of lysosome/late endosome {Gallus gallus},
partial (14%)
Length = 652
Score = 28.1 bits (61), Expect = 3.3
Identities = 17/46 (36%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Frame = +1
Query: 46 RENFGNSDL-PPPPFFTLEDRSDFSPESGILDYQMSPETKSDHKTP 90
R+NF N L PPPP ++ + S +P + + SP TK TP
Sbjct: 31 RDNFSNPGLDPPPPPYSSSESSQSAPPNESTSLR-SPNTKLYSATP 165
>BQ137054 homologue to PIR|T46107|T461 hypothetical protein T25B15.140 -
Arabidopsis thaliana, partial (11%)
Length = 885
Score = 28.1 bits (61), Expect = 3.3
Identities = 13/32 (40%), Positives = 17/32 (52%)
Frame = -3
Query: 115 RGVQQNQQGSLSLNWWSSPAKSGGEQEEKGRG 146
R VQ+ S + WW +SGGE +GRG
Sbjct: 160 REVQRGWSWS*FVRWWWGVMRSGGEGGREGRG 65
>TC83704 similar to PIR|T05005|T05005 hypothetical protein T19P19.70 -
Arabidopsis thaliana, partial (15%)
Length = 716
Score = 27.7 bits (60), Expect = 4.3
Identities = 13/32 (40%), Positives = 18/32 (55%), Gaps = 1/32 (3%)
Frame = +1
Query: 148 PVEGVVQPGALITLPPPPEVAR-PEVQRNSLP 178
P + QP +TLPPPP +++ P R LP
Sbjct: 307 PYQHKEQPPIPVTLPPPPPLSKLPPATREQLP 402
>BQ751277 similar to PIR|T37665|T37 probable t-complex protein 1 epsilon
subunit - fission yeast (Schizosaccharomyces pombe),
partial (39%)
Length = 785
Score = 27.7 bits (60), Expect = 4.3
Identities = 14/44 (31%), Positives = 22/44 (49%)
Frame = -1
Query: 6 QRTPRSGRQSLFFQDLASPVNARRGKFSSPGQSGASSSLWRENF 49
+R PR+ S+ A+ RR S+P + S+S WR N+
Sbjct: 305 RRAPRNAEGSVSTACRAATTT*RRRSSSTPSSARDSNSCWRHNY 174
>BE325816 weakly similar to GP|15028133|gb| unknown protein {Arabidopsis
thaliana}, partial (7%)
Length = 673
Score = 27.3 bits (59), Expect = 5.6
Identities = 21/60 (35%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
Frame = +3
Query: 104 GKSEASTSYALRGVQQNQQGSLSLNWWSSPAKSGGE-------QEEKGRGSPVEGVVQPG 156
GK+++ ++ R + NQQ + AKSGG QEE+GR S G V PG
Sbjct: 72 GKTDSGSNLGKR-TEPNQQNN---------AKSGGNNEERTHHQEERGRKSDSSGSVAPG 221
>TC90113 similar to PIR|C86301|C86301 arginine/serine-rich protein
[imported] - Arabidopsis thaliana, partial (70%)
Length = 1259
Score = 27.3 bits (59), Expect = 5.6
Identities = 35/123 (28%), Positives = 51/123 (41%), Gaps = 10/123 (8%)
Frame = +1
Query: 135 KSGGEQEEKGRGSPVEGVVQPGALITLPPPPEVARPE-VQRNSLPAGNLNEEEWVTVYGF 193
KS E +GR P LPPPP+ RP R P + E + V
Sbjct: 151 KSSAEPARRGRSPP------------LPPPPQSKRPSPPPRKPSP---VRESLVLHVEKL 285
Query: 194 SPNDTNLVLRE-FEKCGEIL-------KHVPGPRDANWMHILYQNRSAAQNA-LNKNGIQ 244
S N L+E F GE++ + V P+ ++H ++ R A+ A L +G Q
Sbjct: 286 SRNVNEGHLKEIFSNFGEVVSVELVMDRAVNLPKGYGYVH--FKTRGEAEKALLYMDGAQ 459
Query: 245 ING 247
I+G
Sbjct: 460 IDG 468
>TC88498 similar to GP|21304712|gb|AAM45475.1 ethylene-responsive element
binding protein 1 {Glycine max}, partial (45%)
Length = 902
Score = 27.3 bits (59), Expect = 5.6
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 68 FSPESGILDYQMSPETKSDHKTPIQTPNREFSTP 101
F S + YQ SP KSD+++ RE +TP
Sbjct: 144 FDDSSFLTSYQPSPSPKSDNESDTIVEAREVNTP 245
>TC76664 weakly similar to PIR|T08541|T08541 hypothetical protein F27B13.40
- Arabidopsis thaliana, partial (38%)
Length = 1860
Score = 27.3 bits (59), Expect = 5.6
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +3
Query: 60 FTLEDRSDFSPESGILDYQMSPETKSD 86
F + +R+ S SGIL SPETKS+
Sbjct: 960 FLINNRNKISRRSGILRRVFSPETKSE 1040
>AW697008 similar to GP|2981301|gb|A 25-hydroxyvitamin D3 24-hydroxylase
{Gallus gallus}, partial (3%)
Length = 807
Score = 26.9 bits (58), Expect = 7.3
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = -2
Query: 145 RGSPVEGVVQPGALITLPPPPEVARP 170
R +P + VV L +PPPP ARP
Sbjct: 758 RAAPCDPVVIHSPLNAIPPPPPSARP 681
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.129 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,809,530
Number of Sequences: 36976
Number of extensions: 116263
Number of successful extensions: 593
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 593
length of query: 247
length of database: 9,014,727
effective HSP length: 94
effective length of query: 153
effective length of database: 5,538,983
effective search space: 847464399
effective search space used: 847464399
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC138130.21