BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138087.2 + phase: 0 /pseudo
(296 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80259 similar to GP|12322022|gb|AAG51060.1 unknown protein; 11... 238 3e-63
TC81522 similar to PIR|T49916|T49916 hypothetical protein T24H18... 97 6e-21
TC80385 similar to PIR|T49916|T49916 hypothetical protein T24H18... 95 3e-20
TC88880 similar to PIR|T49916|T49916 hypothetical protein T24H18... 71 7e-15
CB065371 similar to PIR|T49916|T499 hypothetical protein T24H18.... 70 1e-12
BF635588 similar to GP|12322022|gb| unknown protein; 11168-13185... 39 1e-07
TC81359 weakly similar to GP|5091614|gb|AAD39602.1| Contains a P... 37 0.009
TC89754 similar to GP|21618273|gb|AAM67323.1 unknown {Arabidopsi... 34 0.078
TC80766 similar to GP|15292967|gb|AAK93594.1 unknown protein {Ar... 31 0.50
TC81023 similar to GP|2190542|gb|AAB60906.1| F5I14.5 gene produc... 31 0.66
TC91772 weakly similar to GP|17380998|gb|AAL36311.1 unknown prot... 30 0.86
BQ151380 homologue to GP|1504034|dbj| KIAA0227 {Homo sapiens}, p... 29 1.9
TC80199 weakly similar to GP|21593112|gb|AAM65061.1 unknown {Ara... 29 1.9
BF639306 similar to GP|12322022|gb| unknown protein; 11168-13185... 29 1.9
BF641573 similar to GP|12324328|gb| putative ATPase; 52924-55985... 28 3.3
TC77789 weakly similar to GP|6671937|gb|AAF23197.1| unknown prot... 28 3.3
TC88061 homologue to SP|Q43117|KPYA_RICCO Pyruvate kinase isozym... 28 4.3
TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog... 28 4.3
BG648345 similar to PIR|T04746|T047 hypothetical protein T16H5.1... 28 5.6
TC89077 weakly similar to GP|21740511|emb|CAD41490. OSJNBa0029H0... 28 5.6
>TC80259 similar to GP|12322022|gb|AAG51060.1 unknown protein; 11168-13185
{Arabidopsis thaliana}, partial (43%)
Length = 1026
Score = 238 bits (606), Expect = 3e-63
Identities = 134/185 (72%), Positives = 141/185 (75%), Gaps = 3/185 (1%)
Frame = +2
Query: 1 MEVQSHQLGR-CILCCLVCF*--SSVRCSYLESLITELGIELRVSDEEHRELLGRVNSD* 57
MEVQ HQL + L F S E LITEL ELRVSD+EHRELLGRVNSD
Sbjct: 371 MEVQIHQLEQDAYSAVLRAFKAQSDALTWEKEGLITELRKELRVSDDEHRELLGRVNSDE 550
Query: 58 IIHRTRDWRETGIYQPPGCTTSQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSS 117
IIHR RDWR+TGIYQPP TTSQPVHDILPSPTVSAS KKPKTSHS GQSLPGLSS
Sbjct: 551 IIHRIRDWRQTGIYQPPRRTTSQPVHDILPSPTVSASRKKPKTSHS-----GQSLPGLSS 715
Query: 118 VKPVQCASTGPTGARHFSNHNSSSNLNAPAEEAQFDPLIGKKVWTRWPEDSHFYEAVITD 177
VKPVQ AST PTGARHF+N NSSSNLNAPA DPLIGK+VWT WPED+HFYEA+ITD
Sbjct: 716 VKPVQYAST-PTGARHFANRNSSSNLNAPA-----DPLIGKRVWTXWPEDNHFYEALITD 877
Query: 178 YNPDE 182
YN E
Sbjct: 878 YNHAE 892
>TC81522 similar to PIR|T49916|T49916 hypothetical protein T24H18.190 -
Arabidopsis thaliana, partial (24%)
Length = 817
Score = 97.4 bits (241), Expect = 6e-21
Identities = 48/77 (62%), Positives = 60/77 (77%)
Frame = +3
Query: 29 ESLITELGIELRVSDEEHRELLGRVNSD*IIHRTRDWRETGIYQPPGCTTSQPVHDILPS 88
ESLITEL ELR+S+EEHRELLGRVN+D +I R R+WR+TG +QP ++ +HD +PS
Sbjct: 414 ESLITELRKELRLSNEEHRELLGRVNADDVIRRIREWRQTGGHQPSVLSSGHVIHDSVPS 593
Query: 89 PTVSASNKKPKTSHSVP 105
PTVSAS KK K + SVP
Sbjct: 594 PTVSASRKKQKVTPSVP 644
>TC80385 similar to PIR|T49916|T49916 hypothetical protein T24H18.190 -
Arabidopsis thaliana, partial (27%)
Length = 848
Score = 95.1 bits (235), Expect = 3e-20
Identities = 64/146 (43%), Positives = 76/146 (51%), Gaps = 38/146 (26%)
Frame = +2
Query: 29 ESLITELGIELRVSDEEHRELLGRVNSD*IIHRTRDWRETGIYQPPGCTTSQPVHDILPS 88
ESLITEL ELR+S+EEHRELLGRVN+D I R R+WR+TG +Q +T Q +HD +PS
Sbjct: 401 ESLITELRKELRLSNEEHRELLGRVNADDTIRRIREWRQTGGHQTGMLSTGQALHDSIPS 580
Query: 89 PTVSASNKKPKTSHSVP--------PSL------------------------------GQ 110
PTVSAS KK K + VP PS GQ
Sbjct: 581 PTVSASRKKQKITPPVPSRSFGGPSPSFHPQTVTAPHQPSSSAKRGSGPGSKGKKQKPGQ 760
Query: 111 SLPGLSSVKPVQCASTGPTGARHFSN 136
LPG+SSVK Q +GP G N
Sbjct: 761 ILPGVSSVK--QFPPSGPGGKNQVPN 832
>TC88880 similar to PIR|T49916|T49916 hypothetical protein T24H18.190 -
Arabidopsis thaliana, partial (25%)
Length = 954
Score = 70.9 bits (172), Expect(2) = 7e-15
Identities = 51/136 (37%), Positives = 69/136 (50%), Gaps = 13/136 (9%)
Frame = +2
Query: 147 AEEAQFDPLIGKKVWTRWPEDSHFYEAVITDYNPDE*HVHILQG*YSLLEVSLG*YNIT- 205
AE A DPL+GKKV TRWP+D++FYEAVITDYNP + +H L + SL N++
Sbjct: 95 AEGASIDPLVGKKVRTRWPDDNNFYEAVITDYNPVD-RLHNLVYDMGSVNESLEWVNLSE 271
Query: 206 ------------*GYTMGR*RSGHTT*RLTQRARPWS*EILWLGAGRGKGHPRFHSRKEL 253
G SG R R GAGRG+G + S+K+L
Sbjct: 272 ISPEDIQWVGEEPGINHRFGGSGRGVNRSVGRDNV-------PGAGRGRGGTKGQSKKDL 430
Query: 254 MPPQDGIGNRVPNGIK 269
+P Q+GIG + + I+
Sbjct: 431 LPSQNGIGKKALDDIQ 478
Score = 26.6 bits (57), Expect(2) = 7e-15
Identities = 10/28 (35%), Positives = 20/28 (70%)
Frame = +3
Query: 269 KWKGFSLQVIQILWSLRKQSRC*KLLNE 296
+WKG S+Q+I + LRK + *+++++
Sbjct: 507 RWKGCSVQIILMYMKLRKPRKY*RIMSK 590
>CB065371 similar to PIR|T49916|T499 hypothetical protein T24H18.190 -
Arabidopsis thaliana, partial (7%)
Length = 671
Score = 70.1 bits (170), Expect = 1e-12
Identities = 34/35 (97%), Positives = 35/35 (99%)
Frame = -2
Query: 203 NIT*GYTMGR*RSGHTT*RLTQRARPWS*EILWLG 237
+IT*GYTMGR*RSGHTT*RLTQRARPWS*EILWLG
Sbjct: 106 DIT*GYTMGR*RSGHTT*RLTQRARPWS*EILWLG 2
>BF635588 similar to GP|12322022|gb| unknown protein; 11168-13185
{Arabidopsis thaliana}, partial (18%)
Length = 668
Score = 38.9 bits (89), Expect(2) = 1e-07
Identities = 17/19 (89%), Positives = 17/19 (89%)
Frame = +2
Query: 10 RCILCCLVCF*SSVRCSYL 28
RCIL CL CF*SSVRCSYL
Sbjct: 512 RCILRCLACF*SSVRCSYL 568
Score = 33.9 bits (76), Expect(2) = 1e-07
Identities = 15/17 (88%), Positives = 16/17 (93%)
Frame = +1
Query: 40 RVSDEEHRELLGRVNSD 56
RVSD+EHRELLGR NSD
Sbjct: 610 RVSDDEHRELLGRGNSD 660
>TC81359 weakly similar to GP|5091614|gb|AAD39602.1| Contains a PF|00561
alpha/beta hydrolase fold domain. {Arabidopsis
thaliana}, partial (9%)
Length = 436
Score = 37.0 bits (84), Expect = 0.009
Identities = 19/44 (43%), Positives = 26/44 (58%), Gaps = 4/44 (9%)
Frame = +2
Query: 78 TSQPVHDILPSPTVSASNKKP----KTSHSVPPSLGQSLPGLSS 117
TS P+H +LP+PT S+SN P + S PPSL + +SS
Sbjct: 92 TSSPLHPLLPTPTTSSSNPSP*FQSTITSSPPPSLSHTSSTISS 223
>TC89754 similar to GP|21618273|gb|AAM67323.1 unknown {Arabidopsis
thaliana}, partial (88%)
Length = 759
Score = 33.9 bits (76), Expect = 0.078
Identities = 21/77 (27%), Positives = 33/77 (42%)
Frame = +2
Query: 66 RETGIYQPPGCTTSQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCAS 125
++T +PP P H +PS T SAS+ P ++H S P +S
Sbjct: 68 QKTRFNKPPPSPIHPPPHQSIPSSTSSASSPSPSSAHHASVSNSHPSPSPPPTSSSPSSS 247
Query: 126 TGPTGARHFSNHNSSSN 142
+ + + S+ SSSN
Sbjct: 248 SPTSWSSPASSTTSSSN 298
>TC80766 similar to GP|15292967|gb|AAK93594.1 unknown protein {Arabidopsis
thaliana}, partial (59%)
Length = 785
Score = 31.2 bits (69), Expect = 0.50
Identities = 18/61 (29%), Positives = 26/61 (42%)
Frame = +3
Query: 87 PSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCASTGPTGARHFSNHNSSSNLNAP 146
PSP + + P +S + PP + P S P Q T P H NH+ S + P
Sbjct: 108 PSPPSTTTPSNPSSSQNPPPPTAVNSPQNSKSHPHQ--QTSPQPQSHPDNHHLKSPMQKP 281
Query: 147 A 147
+
Sbjct: 282 S 284
>TC81023 similar to GP|2190542|gb|AAB60906.1| F5I14.5 gene product
{Arabidopsis thaliana}, partial (72%)
Length = 839
Score = 30.8 bits (68), Expect = 0.66
Identities = 20/74 (27%), Positives = 30/74 (40%)
Frame = +2
Query: 70 IYQPPGCTTSQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCASTGPT 129
+ +PP +S P H SPTV+ S T+H+ S +P + C + P+
Sbjct: 164 VNKPPPPPSSSPPHTENSSPTVTTSTGHKTTNHASSSSTISFVPSSPNFSLFPCLPSPPS 343
Query: 130 GARHFSNHNSSSNL 143
H SS L
Sbjct: 344 PVTHPPQVTSSHYL 385
>TC91772 weakly similar to GP|17380998|gb|AAL36311.1 unknown protein
{Arabidopsis thaliana}, partial (17%)
Length = 1237
Score = 30.4 bits (67), Expect = 0.86
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 155 LIGKKVWTRWPEDSHFYEAVITDYN 179
L+GK+V WP+D+ FY+ V+ ++
Sbjct: 878 LVGKRVKVWWPDDNMFYKGVVDSFD 952
>BQ151380 homologue to GP|1504034|dbj| KIAA0227 {Homo sapiens}, partial (4%)
Length = 1185
Score = 29.3 bits (64), Expect = 1.9
Identities = 14/53 (26%), Positives = 27/53 (50%)
Frame = +3
Query: 77 TTSQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCASTGPT 129
TT P+H L + V + +P+T P + ++ P L+ ++P + +T T
Sbjct: 801 TTQCPIHHTLKNNLVPHARAEPRTIPPHPTAPARATPILTQIRPAKKKTTAQT 959
>TC80199 weakly similar to GP|21593112|gb|AAM65061.1 unknown {Arabidopsis
thaliana}, partial (41%)
Length = 934
Score = 29.3 bits (64), Expect = 1.9
Identities = 26/96 (27%), Positives = 36/96 (37%), Gaps = 10/96 (10%)
Frame = +2
Query: 67 ETGIYQPPGCT-TSQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCAS 125
+T IY PP S P + PSP + + P +S PP + P + P S
Sbjct: 194 DTPIYSPPPIIYNSPPPPIVYPSPPPPSPSPPPIVYYSPPPPSPKKPPSANCPPPPSSPS 373
Query: 126 ---------TGPTGARHFSNHNSSSNLNAPAEEAQF 152
TGP G + + N S + P A F
Sbjct: 374 SSSSPYVYMTGPPGNLYPVDVNFSGSGATPNGRASF 481
>BF639306 similar to GP|12322022|gb| unknown protein; 11168-13185
{Arabidopsis thaliana}, partial (11%)
Length = 594
Score = 29.3 bits (64), Expect = 1.9
Identities = 16/26 (61%), Positives = 17/26 (64%), Gaps = 1/26 (3%)
Frame = +2
Query: 4 QSHQLGR-CILCCLVCF*SSVRCSYL 28
Q HQL + L CF*SSVRCSYL
Sbjct: 482 QIHQLEQDAYSAVLACF*SSVRCSYL 559
>BF641573 similar to GP|12324328|gb| putative ATPase; 52924-55985
{Arabidopsis thaliana}, partial (7%)
Length = 656
Score = 28.5 bits (62), Expect = 3.3
Identities = 23/70 (32%), Positives = 30/70 (42%), Gaps = 5/70 (7%)
Frame = -2
Query: 89 PTVSASNKKPKTSHSVPPS----LGQSLPGLSSVK-PVQCASTGPTGARHFSNHNSSSNL 143
PTVS+ N P S S PP + S P + + PVQC S+ N N
Sbjct: 544 PTVSSLNP*PLRSTSAPPDAHSPVSASPPAIETANAPVQCP----------SHPNQPDNY 395
Query: 144 NAPAEEAQFD 153
PA++ FD
Sbjct: 394 Y*PAKQEPFD 365
>TC77789 weakly similar to GP|6671937|gb|AAF23197.1| unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 1149
Score = 28.5 bits (62), Expect = 3.3
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = +1
Query: 79 SQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCAST 126
S P+ PSPT S++ S+S PS SLP S P Q S+
Sbjct: 268 SSPLAPSSPSPTASSTLALTDQSNSKKPSNPSSLPSSPSSPPQQLHSS 411
>TC88061 homologue to SP|Q43117|KPYA_RICCO Pyruvate kinase isozyme A
chloroplast precursor (EC 2.7.1.40). [Castor bean]
{Ricinus communis}, partial (84%)
Length = 1765
Score = 28.1 bits (61), Expect = 4.3
Identities = 19/65 (29%), Positives = 27/65 (41%)
Frame = +1
Query: 74 PGCTTSQPVHDILPSPTVSASNKKPKTSHSVPPSLGQSLPGLSSVKPVQCASTGPTGARH 133
P + S P +PT S+ P TSHS + LS+ +P S T RH
Sbjct: 112 PSTSNSSPPQPQTSTPTPPTSHLPPSTSHS-------AASPLSAPQPPT*QSPTTTAPRH 270
Query: 134 FSNHN 138
+H+
Sbjct: 271 HRHHH 285
>TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog -
cucumber, partial (49%)
Length = 1486
Score = 28.1 bits (61), Expect = 4.3
Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = +3
Query: 71 YQPPGCTTSQPVHDILPSPTVSASNKKPKT-SHSVPPSLGQSLPGLSSV 118
+QPP LP PT + P + SH VPPS SLP S++
Sbjct: 15 HQPPKPAAKTCQQPPLPPPTPPHHHPAPPSPSHPVPPSKHSSLPTPSAL 161
>BG648345 similar to PIR|T04746|T047 hypothetical protein T16H5.10 -
Arabidopsis thaliana, partial (4%)
Length = 788
Score = 27.7 bits (60), Expect = 5.6
Identities = 16/46 (34%), Positives = 21/46 (44%)
Frame = -2
Query: 96 KKPKTSHSVPPSLGQSLPGLSSVKPVQCASTGPTGARHFSNHNSSS 141
KKP +PP +++ GL + C S P S HNSSS
Sbjct: 367 KKPIIDSVLPPDSSKTIQGLR*IISANCFSLKPVSE---SKHNSSS 239
>TC89077 weakly similar to GP|21740511|emb|CAD41490. OSJNBa0029H02.6 {Oryza
sativa}, partial (5%)
Length = 1472
Score = 27.7 bits (60), Expect = 5.6
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +2
Query: 149 EAQFDPLIGKKVWTRWPEDSHFYEAVITDYN 179
E+ + LIG +V WP D FY+ + Y+
Sbjct: 512 ESDAEDLIGCRVKVWWPLDKKFYKGTVQSYD 604
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.335 0.147 0.499
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,150,561
Number of Sequences: 36976
Number of extensions: 178346
Number of successful extensions: 1697
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 1641
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1692
length of query: 296
length of database: 9,014,727
effective HSP length: 95
effective length of query: 201
effective length of database: 5,502,007
effective search space: 1105903407
effective search space used: 1105903407
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC138087.2