BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137838.7 + phase: 0
(692 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86993 similar to GP|13516481|dbj|BAB40450. AMP-binding protein... 720 0.0
TC87211 similar to GP|13516481|dbj|BAB40450. AMP-binding protein... 705 0.0
TC86992 similar to GP|13516481|dbj|BAB40450. AMP-binding protein... 283 8e-78
BE940923 similar to GP|13516481|dbj AMP-binding protein {Arabido... 181 8e-46
BI269959 similar to GP|20805873|gb| long chain acyl-CoA syntheta... 78 1e-14
TC89975 similar to PIR|G96530|G96530 probable acyl CoA synthetas... 41 0.001
TC77772 similar to GP|22136300|gb|AAM91228.1 unknown protein {Ar... 35 0.074
TC77271 similar to PIR|T07929|T07929 probable long-chain-fatty-a... 35 0.13
TC87627 similar to SP|P37221|MAOM_SOLTU NAD-dependent malic enzy... 29 1.3
BF635065 weakly similar to GP|11037008|gb latent nuclear antigen... 30 4.1
TC92340 similar to PIR|D96805|D96805 probable acyl-CoA synthetas... 30 4.1
TC82622 similar to GP|21739238|emb|CAD40993. OSJNBa0072F16.17 {O... 28 9.0
>TC86993 similar to GP|13516481|dbj|BAB40450. AMP-binding protein
{Arabidopsis thaliana}, partial (65%)
Length = 1523
Score = 720 bits (1858), Expect(2) = 0.0
Identities = 374/377 (99%), Positives = 375/377 (99%)
Frame = +3
Query: 85 AQLTLFGITNIWELVFVLMEQLGSTNG*HTEKQVLLGRQ*VLV*FIMESQRVLALGCILL 144
AQLTLFGITNIWELVFVLMEQLGSTNG*HTEKQVLLGRQ*VLV*FIMESQRVLALGCILL
Sbjct: 336 AQLTLFGITNIWELVFVLMEQLGSTNG*HTEKQVLLGRQ*VLV*FIMESQRVLALGCILL 515
Query: 145 TDLSGSLLIMLALHIHTYRCLYMTLSVLMLSNILLTMLWCKLYFVSLKH*IHC*ATCLKF 204
TDLSGSLLIMLALHIHTYRCLYMTLSVLMLSNILLTMLWCKLYF SLKH*IHC*ATCLKF
Sbjct: 516 TDLSGSLLIMLALHIHTYRCLYMTLSVLMLSNILLTMLWCKLYFASLKH*IHC*ATCLKF 695
Query: 205 QLCVLLW*LEV*MIKFHHFHHQMEFKLFHIQSFLVRDAAIFSPFAPQSLRMLQPFAIQVA 264
QLCVLLW*LEV*MIKFHHFHHQMEFKLFHIQSFLVRDAAIFSPFAPQ+LRMLQPFAIQVA
Sbjct: 696 QLCVLLW*LEV*MIKFHHFHHQMEFKLFHIQSFLVRDAAIFSPFAPQNLRMLQPFAIQVA 875
Query: 265 QLEPQRVPS*PMKTSLQMLPGPL*MKNSTLQTFIYPIFLWHTFMSERIKS*QYILAWQWD 324
QLEPQRVPS*PMKTSLQMLPGPL*MKNSTLQTFIYPIFLWHTFMSERIKS*QYILAWQWD
Sbjct: 876 QLEPQRVPS*PMKTSLQMLPGPL*MKNSTLQTFIYPIFLWHTFMSERIKS*QYILAWQWD 1055
Query: 325 STRGII*N*WMT*QL*DLLSSVAFLAYIIEYMLVLLMLLKHLVV*RRGFSMLLTMLKGRH 384
STRGII*N*WMT*QL*DLLSSVAFLAYIIEYMLVLLMLLKHLVV*RRGFSMLLTMLKGRH
Sbjct: 1056STRGII*N*WMT*QL*DLLSSVAFLAYIIEYMLVLLMLLKHLVV*RRGFSMLLTMLKGRH 1235
Query: 385 YCMARTHLQCGTD*FSTR*KKNLEDEFVLWYQVPHLYRLMSWNF*RFALVGE*LKVME*L 444
YCMARTHLQCGTD*FSTR*KKNLEDEFVLWYQVPHLYRLMSWNF*RFALVGE*LKVME*L
Sbjct: 1236YCMARTHLQCGTD*FSTR*KKNLEDEFVLWYQVPHLYRLMSWNF*RFALVGE*LKVME*L 1415
Query: 445 RLLVL*AALITVTDLVV 461
RLLVL*AALITVTDL V
Sbjct: 1416RLLVL*AALITVTDLAV 1466
Score = 88.6 bits (218), Expect(2) = 0.0
Identities = 47/59 (79%), Positives = 50/59 (84%)
Frame = +2
Query: 32 SNPTAGEFFSVLYSQRNCRQESGTCIDPYALL*S**TASLIILKLVHCMIILCAQLTLF 90
S+P+ G VLYSQRNCRQESGTCIDPYALL*S**TASLIILKLVHCMIILC + F
Sbjct: 137 SSPSKG---IVLYSQRNCRQESGTCIDPYALL*S**TASLIILKLVHCMIILCEHYSFF 304
Score = 84.0 bits (206), Expect = 2e-16
Identities = 40/40 (100%), Positives = 40/40 (100%)
Frame = +1
Query: 2 DPTPTAQRRLKAINAHLITAVEDNSSDHLQSNPTAGEFFS 41
DPTPTAQRRLKAINAHLITAVEDNSSDHLQSNPTAGEFFS
Sbjct: 25 DPTPTAQRRLKAINAHLITAVEDNSSDHLQSNPTAGEFFS 144
Score = 27.7 bits (60), Expect(2) = 1.6
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1
Query: 542 RRISLNWHKAS 552
RRISLNWHKAS
Sbjct: 1489 RRISLNWHKAS 1521
Score = 21.6 bits (44), Expect(2) = 1.6
Identities = 10/12 (83%), Positives = 12/12 (99%)
Frame = +3
Query: 532 LAVVSK*LIGRR 543
LAVVSK*LIG++
Sbjct: 1458 LAVVSK*LIGKK 1493
>TC87211 similar to GP|13516481|dbj|BAB40450. AMP-binding protein
{Arabidopsis thaliana}, partial (52%)
Length = 1338
Score = 705 bits (1819), Expect = 0.0
Identities = 369/370 (99%), Positives = 369/370 (99%)
Frame = +1
Query: 323 WDSTRGII*N*WMT*QL*DLLSSVAFLAYIIEYMLVLLMLLKHLVV*RRGFSMLLTMLKG 382
WDSTRGII*N*WMT*QL*DLLSSVAFLAYIIEYMLVLLMLLKHLVV*RRGFSMLLTMLKG
Sbjct: 1 WDSTRGII*N*WMT*QL*DLLSSVAFLAYIIEYMLVLLMLLKHLVV*RRGFSMLLTMLKG 180
Query: 383 RHYCMARTHLQCGTD*FSTR*KKNLEDEFVLWYQVPHLYRLMSWNF*RFALVGE*LKVME 442
RHYCMARTHLQCGTD*FSTR*KKNLEDEFVLWYQVPHLYRLMSWNF*RFALVGE*LKVME
Sbjct: 181 RHYCMARTHLQCGTD*FSTR*KKNLEDEFVLWYQVPHLYRLMSWNF*RFALVGE*LKVME 360
Query: 443 *LRLLVL*AALITVTDLVVTLVLQIQPVR*NL*TFRK*TIHLMISPTPAVKFVSGVPLFF 502
*LRLLVL*AALITVTDLVVTLVLQIQPVR*NL*TFRK*TIHLMISPTPAVKFVSGVPLFF
Sbjct: 361 *LRLLVL*AALITVTDLVVTLVLQIQPVR*NL*TFRK*TIHLMISPTPAVKFVSGVPLFF 540
Query: 503 KVITKMKLKREKSSMKRDGCIPEILGHG*LAVVSK*LIGRRISLNWHKASTLLQRR*KMY 562
KVITKMKLKREKSSMKRDGCIPEILGHG*LAVVSK*LIGRRISLNWHKASTLLQRR*KMY
Sbjct: 541 KVITKMKLKREKSSMKRDGCIPEILGHG*LAVVSK*LIGRRISLNWHKASTLLQRR*KMY 720
Query: 563 MSNAILGHSALYMVIVLILLWYLLSRWILMS*KHGLLPKT*CIMI*HNFAMIQEQRQLFW 622
MSNAIL HSALYMVIVLILLWYLLSRWILMS*KHGLLPKT*CIMI*HNFAMIQEQRQLFW
Sbjct: 721 MSNAILWHSALYMVIVLILLWYLLSRWILMS*KHGLLPKT*CIMI*HNFAMIQEQRQLFW 900
Query: 623 LRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLRGLKRKNTLAKQYLICIAS 682
LRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLRGLKRKNTLAKQYLICIAS
Sbjct: 901 LRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLRGLKRKNTLAKQYLICIAS 1080
Query: 683 SQNPTLLRNP 692
SQNPTLLRNP
Sbjct: 1081SQNPTLLRNP 1110
>TC86992 similar to GP|13516481|dbj|BAB40450. AMP-binding protein
{Arabidopsis thaliana}, partial (23%)
Length = 737
Score = 283 bits (723), Expect(2) = 8e-78
Identities = 149/157 (94%), Positives = 152/157 (95%)
Frame = +1
Query: 32 SNPTAGEFFSVLYSQRNCRQESGTCIDPYALL*S**TASLIILKLVHCMIILCAQLTLFG 91
S+P+ G VLYSQRNCRQESGTCIDPYALL*S**TASLIILKLVHCMIILCAQLTLFG
Sbjct: 235 SSPSKG---IVLYSQRNCRQESGTCIDPYALL*S**TASLIILKLVHCMIILCAQLTLFG 405
Query: 92 ITNIWELVFVLMEQLGSTNG*HTEKQVLLGRQ*VLV*FIMESQRVLALGCILLTDLSGSL 151
ITNIWELVFVLMEQLGSTNG*HTEKQVLLGRQ*VLV*FIMESQRVLALGCILLTDLSGSL
Sbjct: 406 ITNIWELVFVLMEQLGSTNG*HTEKQVLLGRQ*VLV*FIMESQRVLALGCILLTDLSGSL 585
Query: 152 LIMLALHIHTYRCLYMTLSVLMLSNILLTMLWCKLYF 188
LIMLALHIHTYRCLYMTLSVLMLSNIL+TMLWCKLYF
Sbjct: 586 LIMLALHIHTYRCLYMTLSVLMLSNILVTMLWCKLYF 696
Score = 85.9 bits (211), Expect = 5e-17
Identities = 41/41 (100%), Positives = 41/41 (100%)
Frame = +3
Query: 1 MDPTPTAQRRLKAINAHLITAVEDNSSDHLQSNPTAGEFFS 41
MDPTPTAQRRLKAINAHLITAVEDNSSDHLQSNPTAGEFFS
Sbjct: 120 MDPTPTAQRRLKAINAHLITAVEDNSSDHLQSNPTAGEFFS 242
Score = 26.9 bits (58), Expect(2) = 8e-78
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +2
Query: 188 FVSLKH*IHC*AT 200
F SLKH*IHC*AT
Sbjct: 695 FXSLKH*IHC*AT 733
>BE940923 similar to GP|13516481|dbj AMP-binding protein {Arabidopsis
thaliana}, partial (11%)
Length = 541
Score = 181 bits (459), Expect = 8e-46
Identities = 88/88 (100%), Positives = 88/88 (100%)
Frame = +1
Query: 605 IMI*HNFAMIQEQRQLFWLRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLR 664
IMI*HNFAMIQEQRQLFWLRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLR
Sbjct: 121 IMI*HNFAMIQEQRQLFWLRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLR 300
Query: 665 GLKRKNTLAKQYLICIASSQNPTLLRNP 692
GLKRKNTLAKQYLICIASSQNPTLLRNP
Sbjct: 301 GLKRKNTLAKQYLICIASSQNPTLLRNP 384
>BI269959 similar to GP|20805873|gb| long chain acyl-CoA synthetase 6
{Arabidopsis thaliana}, partial (7%)
Length = 267
Score = 77.8 bits (190), Expect = 1e-14
Identities = 46/52 (88%), Positives = 47/52 (89%)
Frame = +2
Query: 435 GE*LKVME*LRLLVL*AALITVTDLVVTLVLQIQPVR*NL*TFRK*TIHLMI 486
GE*L+VME*LRLLVL*AAL TVTDLVVTLVL IQPV *NL*TFR * IHLMI
Sbjct: 107 GE*LRVME*LRLLVL*AALXTVTDLVVTLVLXIQPVX*NL*TFRX*XIHLMI 262
>TC89975 similar to PIR|G96530|G96530 probable acyl CoA synthetase
[imported] - Arabidopsis thaliana, partial (46%)
Length = 1251
Score = 41.2 bits (95), Expect = 0.001
Identities = 39/113 (34%), Positives = 50/113 (43%), Gaps = 4/113 (3%)
Frame = +2
Query: 542 RRISLNWHKASTLLQRR*KMYMSNAILGHSALYMVIVLILLWYLLSRWILMS*KHGLLP- 600
+RISLN+ K +TLL R K + NA+L H YM V W W+ S K LL
Sbjct: 644 KRISLNYLKENTLL*RILKASICNALLYHRFGYMETVSSRSW-----WLWWSLKERLLRI 808
Query: 601 ---KT*CIMI*HNFAMIQEQRQLFWLRWMLLDAKLNLEVLNLQKRSFWWLNHL 650
T + + + I Q FW+ L NLE L+ K W NHL
Sbjct: 809 GQLSTILQVTFNLYVKI*RQDNTFWMNSKALVRNSNLEDLSSSKLFIW--NHL 961
>TC77772 similar to GP|22136300|gb|AAM91228.1 unknown protein {Arabidopsis
thaliana}, partial (62%)
Length = 2554
Score = 35.4 bits (80), Expect = 0.074
Identities = 32/104 (30%), Positives = 50/104 (47%), Gaps = 8/104 (7%)
Frame = +2
Query: 568 LGHSALYMVIVLILLWYLLSRWILMS*KHGLLPKT*CIMI*HNFAMIQEQRQLFWL---- 623
+G+ M+I LI++ L+ +L+ LL C + +N + QR L W
Sbjct: 509 MGNLINLMLICLIIILGLIL*LVLL-----LLICLIC*LRWNNLIFCRIQRVLIWFLRLC 673
Query: 624 ----RWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RL 663
R +LL + LN NL+ + F +NHL W + C L+Q RL
Sbjct: 674 IRLERLLLLRSFLN-GCCNLEMKLFLMMNHLTWSLECSLQQIRL 802
>TC77271 similar to PIR|T07929|T07929 probable long-chain-fatty-acid--CoA
ligase (EC 6.2.1.3) isoform 2 - rape, partial (94%)
Length = 2486
Score = 34.7 bits (78), Expect = 0.13
Identities = 45/189 (23%), Positives = 84/189 (43%), Gaps = 4/189 (2%)
Frame = +2
Query: 502 FKVITKMKLKREKSSMKRDGCIPEILGHG*LAVVSK*LIGRRISLNWHKASTLLQRR*KM 561
F+ T K+ +++ + DG I L G L V + LIGRRI ++ K + L + ++
Sbjct: 1598 FRGTTNAKISQKRFWLM-DGSIQGTLESGNLMEV*RLLIGRRIYSSFPKENMLPLKTWRI 1774
Query: 562 YMSNAIL----GHSALYMVIVLILLWYLLSRWILMS*KHGLLPKT*CIMI*HNFAMIQEQ 617
+++ +L G + + L+LL ++ + + K + P T + + I Q
Sbjct: 1775 FIAKFLLLNLYGFTETVLRPSLLLLLTPANQHLNIGQKKMVYPWTSILSV-----EILGQ 1939
Query: 618 RQLFWLRWMLLDAKLNLEVLNLQKRSFWWLNHLRWKMVC*LRQ*RLRGLKRKNTLAKQYL 677
+ + + L+ K + + L+ K+S HL W + L+ R RG NT+ +
Sbjct: 1940 KVTYLMSSQRLERKRS*KDLSS*KQSILTQFHLIWNVTLSLQHIRRRGPSCLNTIRMLLM 2119
Query: 678 ICIASSQNP 686
C NP
Sbjct: 2120 TCTKVEINP 2146
>TC87627 similar to SP|P37221|MAOM_SOLTU NAD-dependent malic enzyme 62 kDa
isoform mitochondrial precursor (EC 1.1.1.39) (NAD-ME).
[Potato], partial (67%)
Length = 1548
Score = 28.9 bits (63), Expect(2) = 1.3
Identities = 12/32 (37%), Positives = 19/32 (58%)
Frame = -3
Query: 172 LMLSNILLTMLWCKLYFVSLKH*IHC*ATCLK 203
L ++N LT+ K YF+ +H HC + C+K
Sbjct: 550 LCINNPKLTLCTLKSYFIITQHPCHCFSCCIK 455
Score = 20.8 bits (42), Expect(2) = 1.3
Identities = 15/56 (26%), Positives = 24/56 (42%), Gaps = 1/56 (1%)
Frame = -2
Query: 219 KFHHFHHQMEFKLFHIQSFLVRDAAIFSPFAPQSLRMLQPFA-IQVAQLEPQRVPS 273
+F HF HQ + + S + QSL L+ ++V QL+ Q P+
Sbjct: 449 RFQHFQHQQQ---------------LSSAWGSQSLAFLELLQHLEVQQLQHQLFPA 327
>BF635065 weakly similar to GP|11037008|gb latent nuclear antigen {Human
herpesvirus 8}, partial (3%)
Length = 681
Score = 29.6 bits (65), Expect = 4.1
Identities = 15/46 (32%), Positives = 29/46 (62%), Gaps = 3/46 (6%)
Frame = -3
Query: 155 LALHIHTYRCLYMTLSVLMLSNILLTMLWCKLYFVSLKH---*IHC 197
L+ H+ +Y+ ++ L ++ LSN +L L+ +YF+++ H IHC
Sbjct: 607 LSFHVLSYKP*HLQL-IIQLSNYILRQLFLFIYFINIVHLNQLIHC 473
>TC92340 similar to PIR|D96805|D96805 probable acyl-CoA synthetase
62297-59022 [imported] - Arabidopsis thaliana, partial
(52%)
Length = 1110
Score = 29.6 bits (65), Expect = 4.1
Identities = 62/209 (29%), Positives = 88/209 (41%), Gaps = 10/209 (4%)
Frame = +1
Query: 415 YQVPHLYRLMSWNF*RFALVGE*LKVME*LRLLVL*AALITVTDLVVTLVLQIQPVR*NL 474
Y H Y +SW+F V +*+K M RL+++ +LI + L+ L L +
Sbjct: 355 YSAVHQY--LSWSF----PVLQ*VKDMVLPRLVLVGHSLILMIHLLAVLDLLFLAHLLS* 516
Query: 475 *TFRK*TIHLMISPTPAVKFVSGVPLFFKVITKMKLKREKSS---MKR--DGCIPEILGH 529
T K I LM K V + V ++MK KR+K+ MK+ DG G
Sbjct: 517 STGLKVDI*LMTRQCLVAKL*LVVQMSLLVTSRMK-KRQKNHTRLMKKE*DGSTLVT*GG 693
Query: 530 G*LAVVSK*LIGRRISLNWHKASTLLQRR*KMYMSNAILGHS-----ALYMVIVLILLWY 584
V K LI +R LN S R + + +A + L +VIV LLW
Sbjct: 694 FIKMVA*KSLIVKRTLLNCSMESMFPWERLRQLLLSAPMWTI*CCMLILSIVIVWHLLWL 873
Query: 585 LLSRWILMS*KHGLLPKT*CIMI*HNFAM 613
L W K+GL K I + NFA+
Sbjct: 874 LSPHW-----KNGLQIKEFLIQVFQNFAL 945
>TC82622 similar to GP|21739238|emb|CAD40993. OSJNBa0072F16.17 {Oryza
sativa}, partial (78%)
Length = 847
Score = 28.5 bits (62), Expect = 9.0
Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = -2
Query: 147 LSGSLLIMLALHIHTYRCLYMTLSVLMLSNILLTMLWC--KLYFVSLKH*IHC*ATCLKF 204
+S SL+ LHIHT + V ++ +I L +LW + F SL+ + C +C F
Sbjct: 342 ISQSLISCWCLHIHTAFPCWKI*QVQLVHHIGLLILWLS*SIPFSSLRKSLACLYSCRSF 163
Query: 205 Q 205
+
Sbjct: 162 E 160
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.358 0.158 0.552
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,954,975
Number of Sequences: 36976
Number of extensions: 354339
Number of successful extensions: 5507
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 2217
Number of HSP's successfully gapped in prelim test: 264
Number of HSP's that attempted gapping in prelim test: 3127
Number of HSP's gapped (non-prelim): 2747
length of query: 692
length of database: 9,014,727
effective HSP length: 103
effective length of query: 589
effective length of database: 5,206,199
effective search space: 3066451211
effective search space used: 3066451211
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC137838.7