BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137838.2 - phase: 0
(137 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90544 267 9e-73
TC79477 similar to PIR|S56117|S56117 spermatid-specific protein ... 163 2e-41
BF651131 28 1.1
TC79478 similar to GP|18491179|gb|AAL69492.1 unknown protein {Ar... 28 1.1
BQ151366 similar to GP|20146300|dbj hypothetical protein~similar... 28 1.5
TC85918 similar to GP|13562014|gb|AAK30610.1 fibroin 1 {Plectreu... 27 2.0
BG646216 26 5.7
TC88971 similar to SP|P92792|OM20_SOLTU Mitochondrial import rec... 25 7.4
BG586263 SP|P29388|NU5 NADH-ubiquinone oxidoreductase chain 5 (E... 25 7.4
TC88077 similar to GP|15028127|gb|AAK76687.1 unknown protein {Ar... 25 9.7
>TC90544
Length = 656
Score = 267 bits (683), Expect = 9e-73
Identities = 135/135 (100%), Positives = 135/135 (100%)
Frame = +1
Query: 1 MEFQRRQGHCLSERKGQKRKLDEELPEDRQISSAPPTADERAALLVEVANQVTVLESTFT 60
MEFQRRQGHCLSERKGQKRKLDEELPEDRQISSAPPTADERAALLVEVANQVTVLESTFT
Sbjct: 91 MEFQRRQGHCLSERKGQKRKLDEELPEDRQISSAPPTADERAALLVEVANQVTVLESTFT 270
Query: 61 WNEADRAAAKRATHALADLAKNEEVVNVIVEGGAIPALIKHLQAPPVTDCVQKPLPFEHE 120
WNEADRAAAKRATHALADLAKNEEVVNVIVEGGAIPALIKHLQAPPVTDCVQKPLPFEHE
Sbjct: 271 WNEADRAAAKRATHALADLAKNEEVVNVIVEGGAIPALIKHLQAPPVTDCVQKPLPFEHE 450
Query: 121 VEKGSAFALGLLAVK 135
VEKGSAFALGLLAVK
Sbjct: 451 VEKGSAFALGLLAVK 495
>TC79477 similar to PIR|S56117|S56117 spermatid-specific protein T2
precursor - longfin squid, partial (12%)
Length = 636
Score = 163 bits (412), Expect = 2e-41
Identities = 86/126 (68%), Positives = 103/126 (81%), Gaps = 2/126 (1%)
Frame = +2
Query: 12 SERKGQKRKLDEELPEDRQIS--SAPPTADERAALLVEVANQVTVLESTFTWNEADRAAA 69
SE+KGQKRKL+++ + +Q++ S P T D A+L +V V++L S+F+ NE DRA+A
Sbjct: 77 SEKKGQKRKLEQQEEQFQQVTQISLPLTGDALDAVLSDVDQHVSILLSSFSSNEFDRASA 256
Query: 70 KRATHALADLAKNEEVVNVIVEGGAIPALIKHLQAPPVTDCVQKPLPFEHEVEKGSAFAL 129
KRATHALADLAKNEE+VNVIVEGGA+PALIKHLQ P D VQKPLPFEHEVEKGSAFAL
Sbjct: 257 KRATHALADLAKNEEIVNVIVEGGAVPALIKHLQPPTQNDSVQKPLPFEHEVEKGSAFAL 436
Query: 130 GLLAVK 135
GLLAVK
Sbjct: 437 GLLAVK 454
>BF651131
Length = 665
Score = 28.1 bits (61), Expect = 1.1
Identities = 16/52 (30%), Positives = 24/52 (45%)
Frame = +3
Query: 48 VANQVTVLESTFTWNEADRAAAKRATHALADLAKNEEVVNVIVEGGAIPALI 99
V + T+ E F E ATH+ DL N E +N+ +GG + + I
Sbjct: 291 VGSLPTIQEGEFEKEEQYGHKVDEATHSSLDLDTNIEALNMTEDGGLLDSTI 446
>TC79478 similar to GP|18491179|gb|AAL69492.1 unknown protein {Arabidopsis
thaliana}, partial (39%)
Length = 958
Score = 28.1 bits (61), Expect = 1.1
Identities = 19/44 (43%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +2
Query: 62 NEADRAAAKRATHALADLA-KNEEVVNVIVEGGAIPALIKHLQA 104
N D + A AL LA KN+E N IVE A+P LI L++
Sbjct: 71 NLXDTKVQRAAAGALRTLAFKNDENKNQIVECNALPTLILMLRS 202
>BQ151366 similar to GP|20146300|dbj hypothetical protein~similar to Oryza
sativa OSJNBa0058E19.20, partial (9%)
Length = 1137
Score = 27.7 bits (60), Expect = 1.5
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +1
Query: 15 KGQKRKLDEELPEDRQISSAPPTADER 41
+GQ R+ +EE +D APP DER
Sbjct: 430 RGQARRAEEEKTQDGPEREAPPRKDER 510
>TC85918 similar to GP|13562014|gb|AAK30610.1 fibroin 1 {Plectreurys tristis},
partial (8%)
Length = 4117
Score = 27.3 bits (59), Expect = 2.0
Identities = 17/74 (22%), Positives = 32/74 (42%)
Frame = -1
Query: 12 SERKGQKRKLDEELPEDRQISSAPPTADERAALLVEVANQVTVLESTFTWNEADRAAAKR 71
S K +K +L+ E+ +++ P + + E + ++EST NE AAA
Sbjct: 2656 SAEKEEKPELEPLATEEPKVTKEPEKESQEKSEEAEQPKAIAIVESTVEANEEKTAAAIS 2477
Query: 72 ATHALADLAKNEEV 85
+ ++ E V
Sbjct: 2476 EETKIIEVEPTETV 2435
>BG646216
Length = 532
Score = 25.8 bits (55), Expect = 5.7
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = -1
Query: 111 VQKPLPFEHEVEKGSAFALGLLAV 134
+Q P+P E E+EK S LG + +
Sbjct: 298 IQSPIPVETEIEKSSKEKLGSMRI 227
>TC88971 similar to SP|P92792|OM20_SOLTU Mitochondrial import receptor
subunit TOM20 (Translocase of outer membrane 20 kDa
subunit). [Potato], partial (97%)
Length = 956
Score = 25.4 bits (54), Expect = 7.4
Identities = 18/69 (26%), Positives = 31/69 (44%)
Frame = -3
Query: 50 NQVTVLESTFTWNEADRAAAKRATHALADLAKNEEVVNVIVEGGAIPALIKHLQAPPVTD 109
N T+ T NE A + ++ + N+ + ++IVE + LQ PP+
Sbjct: 801 NGYTMKNITTVKNEMISGGANQM*FSVTNNVSNQ-ITSLIVEVEEVVVAGSLLQIPPM*Q 625
Query: 110 CVQKPLPFE 118
C Q+ LP +
Sbjct: 624 CQQQGLPIQ 598
>BG586263 SP|P29388|NU5 NADH-ubiquinone oxidoreductase chain 5 (EC 1.6.5.3).
[Mouse-ear cress] {Arabidopsis thaliana}, partial (18%)
Length = 788
Score = 25.4 bits (54), Expect = 7.4
Identities = 9/17 (52%), Positives = 10/17 (57%)
Frame = +2
Query: 101 HLQAPPVTDCVQKPLPF 117
HL PP DCV P+ F
Sbjct: 374 HLSKPPAPDCVLTPIQF 424
>TC88077 similar to GP|15028127|gb|AAK76687.1 unknown protein {Arabidopsis
thaliana}, partial (27%)
Length = 1130
Score = 25.0 bits (53), Expect = 9.7
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +1
Query: 86 VNVIVEGGAIPALIKHLQAPPVTDCVQKP 114
+++ V G ++PA +KH + PP V P
Sbjct: 985 ISLGVGGPSMPAALKHPRTPPTNPSVDYP 1071
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.131 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,497,299
Number of Sequences: 36976
Number of extensions: 32732
Number of successful extensions: 170
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of query: 137
length of database: 9,014,727
effective HSP length: 86
effective length of query: 51
effective length of database: 5,834,791
effective search space: 297574341
effective search space used: 297574341
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC137838.2