BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137829.8 - phase: 0
(123 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79942 similar to GP|8927657|gb|AAF82148.1| EST gb|N38213 comes... 231 7e-62
AJ501729 similar to GP|8927657|gb| EST gb|N38213 comes from this... 211 4e-56
TC79943 homologue to GP|8927657|gb|AAF82148.1| EST gb|N38213 com... 204 7e-54
CB892607 similar to GP|8927657|gb| EST gb|N38213 comes from this... 103 2e-36
TC87282 similar to GP|21553648|gb|AAM62741.1 Ser Thr specific pr... 27 2.0
TC93332 similar to PIR|T48267|T48267 probable zinc finger protei... 26 4.4
BI264682 homologue to PIR|C82079|C820 pyruvate dehydrogenase E2... 26 4.4
TC88499 similar to GP|2462826|gb|AAB72161.1| unknown protein {Ar... 25 5.7
TC81886 similar to GP|4115933|gb|AAD03443.1| contains similarity... 25 9.8
TC77406 homologue to SP|O82663|DHSA_ARATH Succinate dehydrogenas... 25 9.8
TC89124 similar to GP|20259643|gb|AAM14339.1 unknown protein {Ar... 25 9.8
AW736496 weakly similar to PIR|G72580|G725 hypothetical protein ... 25 9.8
TC89685 similar to PIR|T51397|T51397 hypothetical protein F14F8_... 25 9.8
TC92720 25 9.8
TC79079 similar to GP|19698893|gb|AAL91182.1 splicing factor pu... 25 9.8
TC88406 similar to GP|8777325|dbj|BAA96915.1 bis(5'-adenosyl)-tr... 25 9.8
>TC79942 similar to GP|8927657|gb|AAF82148.1| EST gb|N38213 comes from this
gene. {Arabidopsis thaliana}, partial (9%)
Length = 879
Score = 231 bits (588), Expect = 7e-62
Identities = 111/114 (97%), Positives = 113/114 (98%)
Frame = +3
Query: 1 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELRKIA 60
MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHEL KIA
Sbjct: 129 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELWKIA 308
Query: 61 QKFEDKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP 114
Q+FE+KIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP
Sbjct: 309 QRFEEKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP 470
>AJ501729 similar to GP|8927657|gb| EST gb|N38213 comes from this gene.
{Arabidopsis thaliana}, partial (3%)
Length = 557
Score = 211 bits (538), Expect = 4e-56
Identities = 104/114 (91%), Positives = 107/114 (93%)
Frame = +1
Query: 1 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELRKIA 60
MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNK+ HLPVSGQEGLHEL KIA
Sbjct: 127 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKM------HLPVSGQEGLHELWKIA 288
Query: 61 QKFEDKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP 114
Q+FE+KIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP
Sbjct: 289 QRFEEKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP 450
>TC79943 homologue to GP|8927657|gb|AAF82148.1| EST gb|N38213 comes from
this gene. {Arabidopsis thaliana}, partial (1%)
Length = 494
Score = 204 bits (519), Expect = 7e-54
Identities = 101/114 (88%), Positives = 103/114 (89%)
Frame = +1
Query: 1 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELRKIA 60
MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHEL KIA
Sbjct: 79 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELWKIA 258
Query: 61 QKFEDKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYP 114
Q+FE+KIYTAATSQ MLTMETKSQGTIANNIPPNQVGPSNQPPYP
Sbjct: 259 QRFEEKIYTAATSQ----------MLTMETKSQGTIANNIPPNQVGPSNQPPYP 390
>CB892607 similar to GP|8927657|gb| EST gb|N38213 comes from this gene.
{Arabidopsis thaliana}, partial (1%)
Length = 782
Score = 103 bits (256), Expect(2) = 2e-36
Identities = 51/66 (77%), Positives = 57/66 (86%)
Frame = +3
Query: 9 NQGAEPNMDTSDWRGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELRKIAQKFEDKIY 68
NQGAE NMD SDWRGQ ESRQRIVNKI++TLK HL VSG+EGLHEL KIA +FEDKI+
Sbjct: 87 NQGAELNMDPSDWRGQFPAESRQRIVNKILETLKSHLLVSGEEGLHELWKIA*RFEDKIF 266
Query: 69 TAATSQ 74
T+ATSQ
Sbjct: 267 TSATSQ 284
Score = 64.3 bits (155), Expect(2) = 2e-36
Identities = 31/43 (72%), Positives = 36/43 (83%), Gaps = 2/43 (4%)
Frame = +1
Query: 74 QSDYLRKISLKMLTMETKSQ--GTIANNIPPNQVGPSNQPPYP 114
QSDYL+KIS KMLTMET+SQ +ANN+P NQVGPSN+PP P
Sbjct: 385 QSDYLQKISSKMLTMETRSQVQNPVANNLPSNQVGPSNEPPNP 513
>TC87282 similar to GP|21553648|gb|AAM62741.1 Ser Thr specific protein
kinase-like protein {Arabidopsis thaliana}, partial
(27%)
Length = 1338
Score = 26.9 bits (58), Expect = 2.0
Identities = 17/62 (27%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = -3
Query: 1 MVTNNWRPNQGAEPNMDTSDWRGQLQPESRQRIVNK---IMDTLKRHLPVSGQEGLHELR 57
+V WR Q A N+ S W G + +++++N +M + H+ S +EG
Sbjct: 310 VVGGKWRRTQEAHENLRRSKWGG--GAKKKKKVLNYQW*VMGSDMTHITYSKREGFKNNT 137
Query: 58 KI 59
KI
Sbjct: 136 KI 131
>TC93332 similar to PIR|T48267|T48267 probable zinc finger protein -
Arabidopsis thaliana, partial (20%)
Length = 666
Score = 25.8 bits (55), Expect = 4.4
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +2
Query: 39 DTLKRHLPVSGQEGLH 54
DT RH+ V GQEG+H
Sbjct: 200 DTFARHVGVIGQEGVH 247
>BI264682 homologue to PIR|C82079|C820 pyruvate dehydrogenase E2 component
dihydrolipoamide acetyltransferase VC2413 [imported] -
Vibrio, partial (2%)
Length = 317
Score = 25.8 bits (55), Expect = 4.4
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = +1
Query: 97 ANNIPPNQVGPSNQPPYPGLHLRA 120
A +PP+Q GP + P+PG H R+
Sbjct: 169 AKTVPPSQAGPLFR-PWPGTHGRS 237
>TC88499 similar to GP|2462826|gb|AAB72161.1| unknown protein {Arabidopsis
thaliana}, partial (31%)
Length = 1900
Score = 25.4 bits (54), Expect = 5.7
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = +1
Query: 22 RGQLQPESRQRIVNKIMDTLKRHLPVSGQEGLHELRKIAQKFEDKIYTAATSQSDYLRK 80
R QL ++R+ K++ VS Q + E RK + ED++ A +++Y+R+
Sbjct: 130 RLQLLEAEKKRVHAKVLQARHVAKSVSHQREI-ERRKKKDELEDRLQRAKRQRAEYIRQ 303
>TC81886 similar to GP|4115933|gb|AAD03443.1| contains similarity to human
RNA polymerase II complex component SRB7 (GB:U52960),
partial (36%)
Length = 591
Score = 24.6 bits (52), Expect = 9.8
Identities = 13/54 (24%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +1
Query: 63 FEDKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNIPPNQVGPS--NQPPYP 114
F K+ S +++L + + + GT+ + PPN++ P+ PP+P
Sbjct: 85 FHHKVVLVMDIISQLQEQVNL-IANLALNTVGTLQRDAPPNRLSPNYPEPPPHP 243
>TC77406 homologue to SP|O82663|DHSA_ARATH Succinate dehydrogenase
[ubiquinone] flavoprotein subunit mitochondrial (EC
1.3.5.1) (FP), partial (65%)
Length = 1546
Score = 24.6 bits (52), Expect = 9.8
Identities = 20/57 (35%), Positives = 32/57 (56%)
Frame = +3
Query: 30 RQRIVNKIMDTLKRHLPVSGQEGLHELRKIAQKFEDKIYTAATSQSDYLRKISLKML 86
+QRI++ M R L SG++ + + RKI + IY S+ D+L +SLK+L
Sbjct: 306 QQRILHPEM*FQDR*LWRSGKDVV*DPRKITSIYT*IIYLQMCSRRDFL--VSLKLL 470
>TC89124 similar to GP|20259643|gb|AAM14339.1 unknown protein {Arabidopsis
thaliana}, partial (87%)
Length = 649
Score = 24.6 bits (52), Expect = 9.8
Identities = 13/49 (26%), Positives = 25/49 (50%)
Frame = +3
Query: 52 GLHELRKIAQKFEDKIYTAATSQSDYLRKISLKMLTMETKSQGTIANNI 100
GLH + + ++I T Q D+L ++ + M+ K+Q + NN+
Sbjct: 252 GLHTQVRRLKNVAEEIGTEMKYQKDFLEQLQMTMI----KAQAGVKNNL 386
>AW736496 weakly similar to PIR|G72580|G725 hypothetical protein APE1926 -
Aeropyrum pernix (strain K1), partial (20%)
Length = 645
Score = 24.6 bits (52), Expect = 9.8
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = -2
Query: 100 IPPNQVGPSNQPPYPGLH 117
+PPN S PPY G H
Sbjct: 377 VPPNSDRVSRAPPYSGYH 324
>TC89685 similar to PIR|T51397|T51397 hypothetical protein F14F8_60 -
Arabidopsis thaliana, partial (10%)
Length = 771
Score = 24.6 bits (52), Expect = 9.8
Identities = 16/74 (21%), Positives = 29/74 (38%)
Frame = +1
Query: 27 PESRQRIVNKIMDTLKRHLPVSGQEGLHELRKIAQKFEDKIYTAATSQSDYLRKISLKML 86
P QR++ ++ L +H P+ + H ++Q E + A S + L + M
Sbjct: 469 PVEYQRVILLMLKALLQHTPMDAAQSPHIYAIVSQLVESTLCWEALSVLEALLQSCSSMT 648
Query: 87 TMETKSQGTIANNI 100
G+ N I
Sbjct: 649 GSHPHDIGSFENGI 690
>TC92720
Length = 808
Score = 24.6 bits (52), Expect = 9.8
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +1
Query: 98 NNIPPNQVGPSNQPPYPGLHLRAL 121
N++PP QPPYP L L L
Sbjct: 112 NSLPPAAATQQLQPPYPYLSLPRL 183
>TC79079 similar to GP|19698893|gb|AAL91182.1 splicing factor putative
{Arabidopsis thaliana}, partial (17%)
Length = 1114
Score = 24.6 bits (52), Expect = 9.8
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +1
Query: 102 PNQVGPSNQPPYPGLHL 118
PN +G PP PG+H+
Sbjct: 58 PNSIGLPMPPPRPGMHM 108
>TC88406 similar to GP|8777325|dbj|BAA96915.1
bis(5'-adenosyl)-triphosphatase-like protein
{Arabidopsis thaliana}, partial (94%)
Length = 879
Score = 24.6 bits (52), Expect = 9.8
Identities = 15/39 (38%), Positives = 20/39 (50%)
Frame = -3
Query: 79 RKISLKMLTMETKSQGTIANNIPPNQVGPSNQPPYPGLH 117
RK+ L + T QG+ ++ PNQV PS P LH
Sbjct: 613 RKVFCIPLPLVTSLQGSYLSSPCPNQVFPSIPFPSHPLH 497
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.130 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,348,190
Number of Sequences: 36976
Number of extensions: 33369
Number of successful extensions: 167
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of query: 123
length of database: 9,014,727
effective HSP length: 84
effective length of query: 39
effective length of database: 5,908,743
effective search space: 230440977
effective search space used: 230440977
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC137829.8